Heat stress response and heat stress transcription factors

Heat stress severely affects the annual agricultural production. Heat stress transcription factors (HSFs) represent a critical regulatory juncture in the heat stress response (HSR) of plants. The HsfA1-dependent pathway has been explored well, but the regulatory mechanism of the HsfA1-independent pathway is still under-investigated. In the present research, HsfA4, an important gene of the HsfA1-independent pathway, was isolated from lilies (Lilium longiflorum) using the RACE method, which encodes 435 amino acids. LlHsfA4 contains a typical domain of HSFs and belongs to the HSF A4 family, according to homology comparisons and phylogenetic analysis. LlHsfA4 was mainly expressed in leaves and was induced by heat stress and H2O2 using qRT-PCR and GUS staining in transgenic Arabidopsis. LlHsfA4 had transactivation activity and was located in the nucleus and cytoplasm through a yeast one hybrid system and through transient expression in lily protoplasts. Over expressing LlHsfA4 in Arabidopsis enhanced its basic thermotolerance, but acquired thermotolerance was not achieved. Further research found that heat stress could increase H2O2 content in lily leaves and reduced H2O2 accumulation in transgenic plants, which was consistent with the up-regulation of HSR downstream genes such as Heat stress proteins (HSPs), Galactinol synthase1 (GolS1), WRKY DNA binding protein 30 (WRKY30), Zinc finger of Arabidopsis thaliana 6 (ZAT6) and the ROS-scavenging enzyme Ascorbate peroxidase 2 (APX2). In conclusion, these results indicate that LlHsfA4 plays important roles in heat stress response through regulating the ROS metabolism in lilies.

Download Full-text

Genomic analyses of heat stress transcription factors (HSFs) in simulated drought stress response and storage root deterioration after harvest in cassava

Molecular Biology Reports ◽

10.1007/s11033-020-05673-3 ◽

2020 ◽

Vol 47 (8) ◽

pp. 5997-6007

Author(s):

Jian Zeng ◽

Chunlai Wu ◽

Cheng Wang ◽

Fengfeng Liao ◽

Jiajia Mo ◽

...

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Drought Stress ◽

Stress Response ◽

Storage Root ◽

Genomic Analyses ◽

Simulated Drought ◽

And Storage ◽

Heat Stress Transcription Factors

Download Full-text

Transcriptional Basis for Differential Thermosensitivity of Seedlings of Various Tomato Genotypes

Genes ◽

10.3390/genes11060655 ◽

2020 ◽

Vol 11 (6) ◽

pp. 655

Author(s):

Yangjie Hu ◽

Sotirios Fragkostefanakis ◽

Enrico Schleiff ◽

Stefan Simm

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Stress Response ◽

Elevated Temperatures ◽

Heat Stress Response ◽

Atp Production ◽

Different Temperatures ◽

Related Plant ◽

Shock Proteins ◽

Tomato Genotypes

Transcriptional reprograming after the exposure of plants to elevated temperatures is a hallmark of stress response which is required for the manifestation of thermotolerance. Central transcription factors regulate the stress survival and recovery mechanisms and many of the core responses controlled by these factors are well described. In turn, pathways and specific genes contributing to variations in the thermotolerance capacity even among closely related plant genotypes are not well defined. A seedling-based assay was developed to directly compare the growth and transcriptome response to heat stress in four tomato genotypes with contrasting thermotolerance. The conserved and the genotype-specific alterations of mRNA abundance in response to heat stress were monitored after exposure to three different temperatures. The transcripts of the majority of genes behave similarly in all genotypes, including the majority of heat stress transcription factors and heat shock proteins, but also genes involved in photosynthesis and mitochondrial ATP production. In turn, genes involved in hormone and RNA-based regulation, such as auxin- and ethylene-related genes, or transcription factors like HsfA6b, show a differential regulation that associates with the thermotolerance pattern. Our results provide an inventory of genes likely involved in core and genotype-dependent heat stress response mechanisms with putative role in thermotolerance in tomato seedlings.

Download Full-text

Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways

BMC Genomics ◽

10.1186/1471-2164-8-125 ◽

2007 ◽

Vol 8 (1) ◽

pp. 125 ◽

Cited By ~ 313

Author(s):

William R Swindell ◽

Marianne Huebner ◽

Andreas P Weber

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Stress Response ◽

Heat Shock ◽

Heat Shock Proteins ◽

Transcriptional Profiling ◽

Heat Stress Response ◽

Shock Proteins

Download Full-text

Heat Stress Responses and Thermotolerance in Maize

International Journal of Molecular Sciences ◽

10.3390/ijms22020948 ◽

2021 ◽

Vol 22 (2) ◽

pp. 948

Author(s):

Zhaoxia Li ◽

Stephen H. Howell

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Stress Response ◽

Heat Shock ◽

Stress Responses ◽

Rna Splicing ◽

Optimal Growth ◽

Heat Stress Response ◽

Genes Encoding ◽

Heat Shock Responses

High temperatures causing heat stress disturb cellular homeostasis and impede growth and development in plants. Extensive agricultural losses are attributed to heat stress, often in combination with other stresses. Plants have evolved a variety of responses to heat stress to minimize damage and to protect themselves from further stress. A narrow temperature window separates growth from heat stress, and the range of temperatures conferring optimal growth often overlap with those producing heat stress. Heat stress induces a cytoplasmic heat stress response (HSR) in which heat shock transcription factors (HSFs) activate a constellation of genes encoding heat shock proteins (HSPs). Heat stress also induces the endoplasmic reticulum (ER)-localized unfolded protein response (UPR), which activates transcription factors that upregulate a different family of stress response genes. Heat stress also activates hormone responses and alternative RNA splicing, all of which may contribute to thermotolerance. Heat stress is often studied by subjecting plants to step increases in temperatures; however, more recent studies have demonstrated that heat shock responses occur under simulated field conditions in which temperatures are slowly ramped up to more moderate temperatures. Heat stress responses, assessed at a molecular level, could be used as traits for plant breeders to select for thermotolerance.

Download Full-text

The plant MBF1 protein family: a bridge between stress and transcription

Journal of Experimental Botany ◽

10.1093/jxb/erz525 ◽

2020 ◽

Vol 71 (6) ◽

pp. 1782-1791 ◽

Cited By ~ 1

Author(s):

Fabiola Jaimes-Miranda ◽

Ricardo A Chávez Montes

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Abiotic Stress ◽

Stress Response ◽

Stress Responses ◽

Protein Family ◽

Molecular Function ◽

Developmental Processes ◽

Heat Stress Response ◽

Amount Of Information

Abstract The Multiprotein Bridging Factor 1 (MBF1) proteins are transcription co-factors whose molecular function is to form a bridge between transcription factors and the basal machinery of transcription. MBF1s are present in most archaea and all eukaryotes, and numerous reports show that they are involved in developmental processes and in stress responses. In this review we summarize almost three decades of research on the plant MBF1 family, which has mainly focused on their role in abiotic stress responses, in particular the heat stress response. However, despite the amount of information available, there are still many questions that remain about how plant MBF1 genes, transcripts, and proteins respond to stress, and how they in turn modulate stress response transcriptional pathways.

Download Full-text

A Heat Stress Responsive NAC Transcription Factor Heterodimer Plays Key Roles in Rice Grain Filling

10.1101/2020.02.08.939728 ◽

2020 ◽

Author(s):

Ye Ren ◽

Zhouquan Huang ◽

Hao Jiang ◽

Zhuo Wang ◽

Fengsheng Wu ◽

...

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Heat Stress ◽

Stress Response ◽

Stress Responses ◽

Transcriptional Regulatory Network ◽

Grain Filling ◽

Nac Transcription Factor ◽

Rice Grain ◽

Heat Stress Response

AbstractHigh temperature often leads to the failure of grain filling in rice (Oryza sativa) to cause yield loss, while the mechanism is not well elucidated yet. Here, we report that two seed-specific NAM/ATAF/CUC domain transcription factors, ONAC127 and ONAC129, are responsive to heat stress and involved in the grain filling process of rice. ONAC127 and ONAC129 are dominantly expressed in the pericarp and can form a heterodimer during rice grain filling. CRISPR/Cas9 induced mutants and overexpression lines were then generated to investigate the functions of these two transcription factors. Interestingly, both knock-out and overexpression plants showed incomplete grain filling and shrunken grains, which became more severe under heat stress. Transcriptome analysis revealed that ONAC127 and ONAC129 mainly regulate stimulus response and nutrient transport. ChIP-seq analysis identified that the direct targets of ONAC127 and ONAC129 in developing rice seeds include monosaccharide transporter OsMST6, sugar transporter OsSWEET4, calmodulin-like protein OsMSR2 and AP2/ERF factor OsEATB. These results suggest that ONAC127 and ONAC129 may regulate grain filling through affecting sugar transportation and abiotic stress responses. Overall, this study demonstrates a transcriptional regulatory network involving ONAC127 and ONAC129 and coordinating multiple pathways to modulate seed development and heat stress response at rice reproductive stage.HighlightA NAC transcription factor heterodimer plays vital roles in heat stress response and sugar transportation at rice grain filling stage.

Download Full-text