scholarly journals The Heat Stress Transcription Factor LlHsfA4 Enhanced Basic Thermotolerance through Regulating ROS Metabolism in Lilies (Lilium Longiflorum)

2022 ◽  
Vol 23 (1) ◽  
pp. 572
Author(s):  
Chengpeng Wang ◽  
Yunzhuan Zhou ◽  
Xi Yang ◽  
Bing Zhang ◽  
Fuxiang Xu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Stress Proteins ◽  
Lilium Longiflorum ◽  
Ros Scavenging ◽  
Heat Stress Response ◽  
Ros Metabolism ◽  
Heat Stress Proteins ◽  
Dependent Pathway ◽  
Heat Stress Transcription Factors

Heat stress severely affects the annual agricultural production. Heat stress transcription factors (HSFs) represent a critical regulatory juncture in the heat stress response (HSR) of plants. The HsfA1-dependent pathway has been explored well, but the regulatory mechanism of the HsfA1-independent pathway is still under-investigated. In the present research, HsfA4, an important gene of the HsfA1-independent pathway, was isolated from lilies (Lilium longiflorum) using the RACE method, which encodes 435 amino acids. LlHsfA4 contains a typical domain of HSFs and belongs to the HSF A4 family, according to homology comparisons and phylogenetic analysis. LlHsfA4 was mainly expressed in leaves and was induced by heat stress and H2O2 using qRT-PCR and GUS staining in transgenic Arabidopsis. LlHsfA4 had transactivation activity and was located in the nucleus and cytoplasm through a yeast one hybrid system and through transient expression in lily protoplasts. Over expressing LlHsfA4 in Arabidopsis enhanced its basic thermotolerance, but acquired thermotolerance was not achieved. Further research found that heat stress could increase H2O2 content in lily leaves and reduced H2O2 accumulation in transgenic plants, which was consistent with the up-regulation of HSR downstream genes such as Heat stress proteins (HSPs), Galactinol synthase1 (GolS1), WRKY DNA binding protein 30 (WRKY30), Zinc finger of Arabidopsis thaliana 6 (ZAT6) and the ROS-scavenging enzyme Ascorbate peroxidase 2 (APX2). In conclusion, these results indicate that LlHsfA4 plays important roles in heat stress response through regulating the ROS metabolism in lilies.

Heat stress response and heat stress transcription factors

1998 ◽  
Vol 23 (4) ◽  
pp. 313-329 ◽  
Author(s):  
Klaus-Dieter Scharf ◽  
Ingo Höhfeld ◽  
Lutz Nover
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Stress Response ◽  
Heat Stress Response ◽  
Heat Stress Transcription Factors

Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors

2004 ◽  
Vol 29 (4) ◽  
pp. 471-487 ◽  
Author(s):  
Sanjeev Kumar Baniwal ◽  
Kapil Bharti ◽  
Kwan Yu Chan ◽  
Markus Fauth ◽  
Arnab Ganguli ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Stress Response ◽  
Heat Stress Response ◽  
Heat Stress Transcription Factors

Mining Heat Stress Induced Genes Encoding Heat Shock Proteins (HSPs) and Heat Stress Transcription Factors (HSFs) in Strawberry (Fragaria x ananassa)

2013 ◽  
Vol 446-447 ◽  
pp. 936-941
Author(s):  
Lee Fong Lin ◽  
Shih Yi Liao ◽  
Jui Hung Yen ◽  
Shu Jiau Chiou ◽  
Cheng Yu Lee ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Proteins ◽  
Extreme Temperature ◽  
Rt Pcr ◽  
Reading Frame ◽  
Genes Encoding ◽  
Stress Responsive Genes ◽  
Heat Stress Proteins ◽  
Shock Proteins ◽  
Heat Stress Transcription Factors

Plants have developed a variety of responses to extreme temperature situation in order to minimize damage and maintain cellular homeostasis. Recent research has revealed the involvement of heat stress proteins in thermotolerance in Arabidopsis. However, it is still obscure in strawberry plants. To identify heat stress responsive genes in strawberry (Fragaria x ananassa), we constructed a Transcriptome for strawberry and it was compared to Unigene annotated in several available proteomics, including Nr, COG, KEGC, and Swissport. Nineteen candidates with complete open reading frame (ORF) of 191 predicted genes encoding HSPs or HSFs were further analyzed. RT-PCR assays and heat stress induction analysis for these potential HSPs and HSFs were performed. Our data demonstrate that the prediction of potential heat stress responsive genes from our created Transcriptome for HSPs and HSFs, respectively, correlates well with the subsequent experimental results, indicating the Transcriptome we constructed could be translated into other scientific use.

scholarly journals iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11509
Author(s):  
Jing Wang ◽  
Chengliang Liang ◽  
Sha Yang ◽  
Jingshuang Song ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Proteomic Analysis ◽  
Negative Impact ◽  
Bioinformatic Analysis ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Ros Scavenging ◽  
Heat Stress Response ◽  
Quantitative Proteomic Analysis

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

Single Gene Consistently Associated with Heat Stress Response in Three Distinct Chicken Lines

2017 ◽  
Author(s):  
Xi Lan ◽  
John C. F. Hsieh ◽  
Carl J. Schmidt ◽  
Qing Zhu ◽  
Susan J. Lamont
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Single Gene ◽  
Heat Stress Response

The Heat Stress Response and Diabetes: More Room for Mitochondrial Implication

2016 ◽  
Vol 22 (18) ◽  
pp. 2619-2639 ◽  
Author(s):  
Biljana Miova ◽  
Maja Dimitrovska ◽  
Suzana Dinevska-Kjovkarovska ◽  
Juan V. Esplugues ◽  
Nadezda Apostolova
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Stress Response

scholarly journals Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bin Huang ◽  
Zhinuo Huang ◽  
Ruifang Ma ◽  
Jialu Chen ◽  
Zhijun Zhang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Shock ◽  
Gene Family ◽  
Regulatory Elements ◽  
Moso Bamboo ◽  
Naphthalene Acetic Acid ◽  
Plant Responses ◽  
Heat Stress Response ◽  
Regulatory Pathways

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

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