Intensification of the breeding process in wheat (T. aestivum L.) by using in vitro methods and characterization of the new developed genotypes

2003 ◽  
Vol 31 (3-4) ◽  
pp. 355-361
Author(s):  
Venetsiya R. Milkova ◽  
P. I. Ivanov ◽  
I. Todorov
Keyword(s):  
In Vitro Methods

scholarly journals Comparative Assessment of In Vitro and In Silico Methods for Aerodynamic Characterization of Powders for Inhalation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1831
Author(s):  
Jelisaveta Ignjatović ◽  
Tijana Šušteršič ◽  
Aleksandar Bodić ◽  
Sandra Cvijić ◽  
Jelena Đuriš ◽  
...  
Keyword(s):  
In Silico ◽  
Aerodynamic Performance ◽  
Dry Powders ◽  
In Vitro Methods ◽  
Discrete Phase Modeling ◽  
Discrete Phase ◽  
Computational Fluid Dynamics Cfd ◽  
In Silico Methods

In vitro assessment of dry powders for inhalation (DPIs) aerodynamic performance is an inevitable test in DPI development. However, contemporary trends in drug development also implicate the use of in silico methods, e.g., computational fluid dynamics (CFD) coupled with discrete phase modeling (DPM). The aim of this study was to compare the designed CFD-DPM outcomes with the results of three in vitro methods for aerodynamic assessment of solid lipid microparticle DPIs. The model was able to simulate particle-to-wall sticking and estimate fractions of particles that stick or bounce off the inhaler’s wall; however, we observed notable differences between the in silico and in vitro results. The predicted emitted fractions (EFs) were comparable to the in vitro determined EFs, whereas the predicted fine particle fractions (FPFs) were generally lower than the corresponding in vitro values. In addition, CFD-DPM predicted higher mass median aerodynamic diameter (MMAD) in comparison to the in vitro values. The outcomes of different in vitro methods also diverged, implying that these methods are not interchangeable. Overall, our results support the utility of CFD-DPM in the DPI development, but highlight the need for additional improvements in these models to capture all the key processes influencing aerodynamic performance of specific DPIs.

Beta-secretase as a target for Alzheimer’s disease drug discovery: an overview of in vitro methods for characterization of inhibitors

2011 ◽  
Vol 400 (7) ◽  
pp. 1979-1996 ◽  
Author(s):  
Francesca Mancini ◽  
Angela De Simone ◽  
Vincenza Andrisano
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Drug Discovery ◽  
In Vitro Methods ◽  
Beta Secretase

scholarly journals Insights into a Protein-Nanoparticle System by Paramagnetic Perturbation NMR Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5187
Author(s):  
Yamanappa Hunashal ◽  
Cristina Cantarutti ◽  
Sofia Giorgetti ◽  
Loredana Marchese ◽  
Federico Fogolari ◽  
...  
Keyword(s):  
Conformational Exchange ◽  
Resonance Spectroscopy ◽  
Equilibrium Conditions ◽  
In Vitro Methods ◽  
Spin Resonance ◽  
Potential Applications ◽  
Nanoparticle System ◽  
Nitroxide Probe

Background: The interaction between proteins and nanoparticles is a very relevant subject because of the potential applications in medicine and material science in general. Further interest derives from the amyloidogenic character of the considered protein, β2-microglobulin (β2m), which may be regarded as a paradigmatic system for possible therapeutic strategies. Previous evidence showed in fact that gold nanoparticles (AuNPs) are able to inhibit β2m fibril formation in vitro. Methods: NMR (Nuclear Magnetic Resonance) and ESR (Electron Spin Resonance) spectroscopy are employed to characterize the paramagnetic perturbation of the extrinsic nitroxide probe Tempol on β2m in the absence and presence of AuNPs to determine the surface accessibility properties and the occurrence of chemical or conformational exchange, based on measurements conducted under magnetization equilibrium and non-equilibrium conditions. Results: The nitroxide perturbation analysis successfully identifies the protein regions where protein-protein or protein-AuNPs interactions hinder accessibility or/and establish exchange contacts. These information give interesting clues to recognize the fibrillation interface of β2m and hypothesize a mechanism for AuNPs fibrillogenesis inhibition. Conclusions: The presented approach can be advantageously applied to the characterization of the interface in protein-protein and protein-nanoparticles interactions.

Anti-amnesic activity screening of the seed ethanol extracts of Turkish Paeonia taxa by in vitro methods

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
D Sevim ◽  
FS Senol ◽  
I Orhan ◽  
B Şener ◽  
E Kaya
Keyword(s):  
In Vitro Methods ◽  
Activity Screening ◽  
Ethanol Extracts

Analysis of antidepressant properties of some Papaver species by in vivo and in vitro methods

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
OML Bayazeid ◽  
F Yalcin ◽  
M İlhan ◽  
H Karahan ◽  
E Kupeli-Akkol ◽  
...  
Keyword(s):  
In Vitro Methods

Biological and physico-chemical characterization of ultra high diluted Hydrastis canadensis and in vitro studies on mammalian cells

2017 ◽  
Vol 262 (02) ◽  
pp. 2-76
Author(s):  
N Chandrakar ◽  
MK Temgire ◽  
SG Kane ◽  
AK Suresh ◽  
J Bellare
Keyword(s):  
Mammalian Cells ◽  
Chemical Characterization ◽  
In Vitro Studies ◽  
Hydrastis Canadensis ◽  
Physico Chemical

Assays for Phospholipid-Dependent Formation of Thrombin and Xa: A Potential Method for Quantifying Lupus Anticoagulant Activity

1991 ◽  
Vol 66 (04) ◽  
pp. 453-458 ◽  
Author(s):  
John T Brandt
Keyword(s):  
Specific Activity ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Clinical Importance ◽  
Potential Method ◽  
Quantitative Expression ◽  
Increased Risk ◽  
Apparent Association

SummaryLupus anticoagulants (LAs) are antibodies which interfere with phospholipid-dependent procoagulant reactions. Their clinical importance is due to their apparent association with an increased risk of thrombo-embolic disease. To date there have been few assays for quantifying the specific activity of these antibodies in vitro and this has hampered attempts to purify and characterize these antibodies. Methods for determining phospholipid-dependent generation of thrombin and factor Xa are described. Isolated IgG fractions from 7 of 9 patients with LAs were found to reproducibly inhibit enzyme generation in these assay systems, permitting quantitative expression of inhibitor activity. Different patterns of inhibitory activity, based on the relative inhibition of thrombin and factor Xa generation, were found, further substantiating the known heterogeneity of these antibodies. These systems may prove helpful in further purification and characterization of LAs.

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