Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns

2019 ◽  
Vol 14 (4) ◽  
pp. 355-364
Author(s):  
Salima Sadeghi ◽  
Kwai Lin Thong ◽  
Lay Ching Chai
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Incubation Temperature ◽  
Detection Efficiency ◽  
Selective Media ◽  
Pathogenic Vibrio ◽  
Enrichment Step

scholarly journals Isolation and selection of bacteria against shrimp pathogenic Vibrio parahaemolyticus from shrimp pond water on Duyen Hai district, Tra Vinh province

2020 ◽  
Vol 10 (1) ◽  
pp. 43-52
Author(s):  
Tran Vu Phuong ◽  
Dang Thi Ngoc Thanh ◽  
Cao Ngoc Diep
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Pond Water ◽  
Shrimp Farming ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Shrimp Pond ◽  
Pathogenic Vibrio ◽  
The Status

Antibiotic has frequently been used in the shrimp-farming process in Vietnam. This leads to the status that antibiotic-resistant bacteria and products do not receive in the market. Bacteria had the resistant ability to pathogenic bacteria in water, and they have an important role in sustainable aquaculture. This study aimed to isolate and select good bacterial strains against Vibrio parahaemolyticus, pathogenic bacteria, on shrimp from 8 samples of shrimp pond water at 3 villages Ngu Lac, Phuoc An and Long Toan of Duyen Hai district, Tra Vinh province on NB agar medium. As a result, fifty-nine bacterial isolates were isolated and 10/59 isolates (16.95%) were identified as resistant to Vibrio parahaemolyticus by the well diffusion method. In 10 isolates, there were 7 isolates had good resistance to select for PCR technique and sequencing. The result indicated that these seven strains, including DH1m, DH2f, DH4d, DH8i, DH8m, DH8n, belonged to Bacilli and DH1n strain belonged to Streptomyces sp.

scholarly journals Longitudinal Study of Total and Pathogenic Vibrio parahaemolyticus (tdh+ and/or trh+) in Two Natural Extraction Areas of Mytilus chilensis in Southern Chile

2021 ◽  
Vol 12 ◽  
Author(s):  
Cristina Bacian ◽  
Cristobal Verdugo ◽  
Katherine García ◽  
Josu Perez-Larruscain ◽  
Ignacio de Blas ◽  
...  
Keyword(s):  
Water Temperature ◽  
Vibrio Parahaemolyticus ◽  
River Estuary ◽  
Environmental Parameters ◽  
Economic Losses ◽  
Pcr Analysis ◽  
Potential Hazard ◽  
Detection Rates ◽  
Pathogenic Vibrio ◽  
Interaction Factor

Vibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. Although different studies have focused on its pattern of variation over time, knowledge about the environmental factors driving the dynamics of this pathogen, within the Chilean territory, is still lacking. This study determined the prevalence of total and pathogenic V. parahaemolyticus strains (tdh and/or trh genes) in mussels (Mytilus chilensis) collected from two natural growing areas between 2017 and 2018, using selective agar and PCR analysis. V. parahaemolyticus was detected in 45.6% (93/204) of pooled samples from the Valdivia River Estuary. The pathogenic strains carrying the tdh and/or trh gene were detected in 11.8% (24/204): tdh in 9.8% (20/204), trh in 0.5% (1/204), and 1.5% (3/204) presented both genes. In Reloncaví Fjord, V. parahaemolyticus was detected in 14.4% (30/209) of the samples, pathogenic V. parahaemolyticus carrying the trh gene was detected in 0.5% (1/209) of the samples, while the tdh gene was not detected in the samples from this area. The total count of mauve-purple colonies typical of V. parahaemolyticus on CHROMagar was positively associated by multivariate analysis with area, water temperature, and salinity. Similarly, V. parahaemolyticus detection rates by PCR had a positive correlation with the area and water temperature. The chances of detecting total V. parahaemolyticus in the Valdivia River Estuary are significantly higher than in the Reloncaví Fjord, but inversely, during spring-summer months, the interaction factor between the area and temperature indicated that the chances of detecting V. parahaemolyticus are higher in the Reloncaví Fjord. Interestingly, this period coincides with the season when commercial and natural-growing shellfish are harvested. On the other hand, pathogenic V. parahaemolyticus tdh+ was significantly correlated with an increase of water temperature. These environmental parameters could be used to trigger a warning on potential hazard, which would influence human health and economic losses in aquaculture systems.

Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance

2020 ◽  
Vol 160 ◽  
pp. 111551
Author(s):  
Sreejith V. Narayanan ◽  
Toms C. Joseph ◽  
Shaheer Peeralil ◽  
Reshmi Koombankallil ◽  
Murugadas Vaiyapuri ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vibrio Parahaemolyticus ◽  
Shrimp Aquaculture ◽  
High Genetic Diversity ◽  
Pathogenic Vibrio ◽  
Tropical Shrimp

Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters

2008 ◽  
Vol 128 (2) ◽  
pp. 354-361 ◽  
Author(s):  
Salina Parveen ◽  
Kumidini A. Hettiarachchi ◽  
John C. Bowers ◽  
Jessica L. Jones ◽  
Mark L. Tamplin ◽  
...  
Keyword(s):  
Chesapeake Bay ◽  
Vibrio Parahaemolyticus ◽  
Seasonal Distribution ◽  
Pathogenic Vibrio

Complete genome sequence of virulent bacteriophage SHOU24, which infects foodborne pathogenic Vibrio parahaemolyticus

2014 ◽  
Vol 159 (11) ◽  
pp. 3089-3093 ◽  
Author(s):  
Lin Yuan ◽  
Zelin Cui ◽  
Yanchun Wang ◽  
Xiaokui Guo ◽  
Yong Zhao
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Vibrio

Presence of pathogenic Vibrio Parahaemolyticus in waters and seafood from the Tunisian Sea

2013 ◽  
Vol 29 (8) ◽  
pp. 1341-1348 ◽  
Author(s):  
Sadok Khouadja ◽  
Elisabetta Suffredini ◽  
Matteo Spagnoletti ◽  
Luciana Croci ◽  
Mauro M. Colombo ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pathogenic Vibrio

Evaluation of an Alkaline Phosphatase–Labeled Oligonucleotide Probe for the Detection and Enumeration of the Thermostable-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus

2006 ◽  
Vol 69 (11) ◽  
pp. 2770-2772 ◽  
Author(s):  
JESSICA L. NORDSTROM ◽  
RACHEL RANGDALE ◽  
MICHAEL C. L. VICKERY ◽  
ANDREA M. B. PHILLIPS ◽  
SHELLEY L. MURRAY ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Vibrio Parahaemolyticus ◽  
Oligonucleotide Probe ◽  
The United States ◽  
Bacterial Strains ◽  
Specific Detection ◽  
Colony Hybridization ◽  
The United Kingdom ◽  
Pathogenic Vibrio ◽  
Reliable Methods

Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase–labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.

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