Occurrence of pathogenic Vibrio parahaemolyticus
 in seafood distribution channels and their antibiotic resistance profiles in S. Korea

Vibrio parahaemolyticus is a Gram-negative bacterium that is a natural inhabitant of the marine habitat. V. parahaemolyticus is a human foodborne pathogen linked to the consumption of contaminated raw and undercooked seafood. V. parahaemolyticus pathogenicity has been linked to the presence of two virulence gene that is thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh). The emergence of antibiotic resistant strain of V. parahaemolyticus is a menace to public health. V. parahaemolyticus is linked to several foodborne diseases in Asian countries including Japan, China and Taiwan and has been acknowledged as the major cause of human gastroenteritis in the United States. The emergence of pathogenic Vibrio species in shellfish in Malaysia requires persistent monitoring and public enlightenment on food safety. Several detection methods based on its virulence factors are used in detecting V. parahaemolyticus. This review will provide an insight on V. parahaemolyticus, its pathogenicity, antibiotic resistance, foodborne outbreaks and detection methods.

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Draft Genome Sequence of the Shrimp Pathogen Vibrio parahaemolyticus ST17.P5-S1, Isolated in Peninsular Malaysia

Microbiology Resource Announcements ◽

10.1128/mra.01053-18 ◽

2018 ◽

Vol 7 (11) ◽

Cited By ~ 2

Author(s):

Sridevi Devadas ◽

Subha Bhassu ◽

Tze Chiew Christie Soo ◽

Fatimah M. Yusoff ◽

Mohamed Shariff

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Vibrio Parahaemolyticus ◽

East Coast ◽

Draft Genome ◽

Antibiotic Resistance Genes ◽

Peninsular Malaysia ◽

Penaeus Vannamei ◽

Content Type ◽

Acute Hepatopancreatic Necrosis Disease

We sequenced the genome of Vibrio parahaemolyticus strain ST17.P5-S1, isolated from Penaeus vannamei cultured in the east coast of Peninsular Malaysia. The strain contains several antibiotic resistance genes and a plasmid encoding the Photorhabdus insect-related (Pir) toxin-like genes, pirAvp and pirBvp, associated with acute hepatopancreatic necrosis disease (AHPND).

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Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns

Journal of Consumer Protection and Food Safety ◽

10.1007/s00003-019-01237-1 ◽

2019 ◽

Vol 14 (4) ◽

pp. 355-364

Author(s):

Salima Sadeghi ◽

Kwai Lin Thong ◽

Lay Ching Chai

Keyword(s):

Vibrio Parahaemolyticus ◽

Incubation Temperature ◽

Detection Efficiency ◽

Selective Media ◽

Pathogenic Vibrio ◽

Enrichment Step

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Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review

Fisheries and Aquatic Sciences ◽

10.1186/s41240-018-0081-4 ◽

2018 ◽

Vol 21 (1) ◽

Cited By ~ 13

Author(s):

Kunbawui Park ◽

Jong Soo Mok ◽

Ji Young Kwon ◽

A Ra Ryu ◽

Song Hee Kim ◽

...

Keyword(s):

Antibiotic Resistance ◽

Vibrio Parahaemolyticus ◽

Food Borne

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Isolation and selection of bacteria against shrimp pathogenic Vibrio parahaemolyticus from shrimp pond water on Duyen Hai district, Tra Vinh province

ENGINEERING AND TECHNOLOGY ◽

10.46223/hcmcoujs.tech.en.10.1.359.2020 ◽

2020 ◽

Vol 10 (1) ◽

pp. 43-52

Author(s):

Tran Vu Phuong ◽

Dang Thi Ngoc Thanh ◽

Cao Ngoc Diep

Keyword(s):

Vibrio Parahaemolyticus ◽

Pathogenic Bacteria ◽

Pond Water ◽

Shrimp Farming ◽

Diffusion Method ◽

Resistant Bacteria ◽

Bacterial Strains ◽

Shrimp Pond ◽

Pathogenic Vibrio ◽

The Status

Antibiotic has frequently been used in the shrimp-farming process in Vietnam. This leads to the status that antibiotic-resistant bacteria and products do not receive in the market. Bacteria had the resistant ability to pathogenic bacteria in water, and they have an important role in sustainable aquaculture. This study aimed to isolate and select good bacterial strains against Vibrio parahaemolyticus, pathogenic bacteria, on shrimp from 8 samples of shrimp pond water at 3 villages Ngu Lac, Phuoc An and Long Toan of Duyen Hai district, Tra Vinh province on NB agar medium. As a result, fifty-nine bacterial isolates were isolated and 10/59 isolates (16.95%) were identified as resistant to Vibrio parahaemolyticus by the well diffusion method. In 10 isolates, there were 7 isolates had good resistance to select for PCR technique and sequencing. The result indicated that these seven strains, including DH1m, DH2f, DH4d, DH8i, DH8m, DH8n, belonged to Bacilli and DH1n strain belonged to Streptomyces sp.

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Longitudinal Study of Total and Pathogenic Vibrio parahaemolyticus (tdh+ and/or trh+) in Two Natural Extraction Areas of Mytilus chilensis in Southern Chile

Frontiers in Microbiology ◽

10.3389/fmicb.2021.621737 ◽

2021 ◽

Vol 12 ◽

Author(s):

Cristina Bacian ◽

Cristobal Verdugo ◽

Katherine García ◽

Josu Perez-Larruscain ◽

Ignacio de Blas ◽

...

Keyword(s):

Water Temperature ◽

Vibrio Parahaemolyticus ◽

River Estuary ◽

Environmental Parameters ◽

Economic Losses ◽

Pcr Analysis ◽

Potential Hazard ◽

Detection Rates ◽

Pathogenic Vibrio ◽

Interaction Factor

Vibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. Although different studies have focused on its pattern of variation over time, knowledge about the environmental factors driving the dynamics of this pathogen, within the Chilean territory, is still lacking. This study determined the prevalence of total and pathogenic V. parahaemolyticus strains (tdh and/or trh genes) in mussels (Mytilus chilensis) collected from two natural growing areas between 2017 and 2018, using selective agar and PCR analysis. V. parahaemolyticus was detected in 45.6% (93/204) of pooled samples from the Valdivia River Estuary. The pathogenic strains carrying the tdh and/or trh gene were detected in 11.8% (24/204): tdh in 9.8% (20/204), trh in 0.5% (1/204), and 1.5% (3/204) presented both genes. In Reloncaví Fjord, V. parahaemolyticus was detected in 14.4% (30/209) of the samples, pathogenic V. parahaemolyticus carrying the trh gene was detected in 0.5% (1/209) of the samples, while the tdh gene was not detected in the samples from this area. The total count of mauve-purple colonies typical of V. parahaemolyticus on CHROMagar was positively associated by multivariate analysis with area, water temperature, and salinity. Similarly, V. parahaemolyticus detection rates by PCR had a positive correlation with the area and water temperature. The chances of detecting total V. parahaemolyticus in the Valdivia River Estuary are significantly higher than in the Reloncaví Fjord, but inversely, during spring-summer months, the interaction factor between the area and temperature indicated that the chances of detecting V. parahaemolyticus are higher in the Reloncaví Fjord. Interestingly, this period coincides with the season when commercial and natural-growing shellfish are harvested. On the other hand, pathogenic V. parahaemolyticus tdh+ was significantly correlated with an increase of water temperature. These environmental parameters could be used to trigger a warning on potential hazard, which would influence human health and economic losses in aquaculture systems.

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