The comparison of patellar tendon?bone autografting and free flexor-tendon autografting in infraspinatus defect of the shoulder: biomechanical and histological evaluation in a sheep model

2004 ◽  
Vol 12 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Mehmet A. Altay ◽  
Muhittin Sener ◽  
Celal Baki ◽  
Ahmet U. Turhan ◽  
Umit Cobanoglu
Keyword(s):  
Patellar Tendon ◽  
Flexor Tendon ◽  
Histological Evaluation ◽  
Sheep Model

scholarly journals Local and systemic inflammation after implantation of a novel iron based porous degradable bone replacement material in sheep model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bernd Wegener ◽  
Maik Behnke ◽  
Stefan Milz ◽  
Volkmar Jansson ◽  
Christian Redlich ◽  
...  
Keyword(s):  
Mechanical Stability ◽  
Bone Substitutes ◽  
Histological Evaluation ◽  
Control Group ◽  
Blood Levels ◽  
Porous Iron ◽  
Sheep Model ◽  
Bone Replacement ◽  
Alloy Particles ◽  
Iron Based

AbstractDespite the high potential of healthy bone to regenerate, the reconstruction of large bone defects remains a challenge. Due to the lack of mechanical stability of existing bone substitutes, recently developed degradable metallic alloys are an interesting alternative providing higher load-bearing capabilities. Degradable iron-based alloys therefore might be an attractive innovation. To test the suitability of a newly-designed iron-based alloy for such applications, an animal experiment was performed. Porous iron-based degradable implants with two different densities and a control group were tested. The implants were positioned in the proximal tibia of Merino sheep. Over a period of 6 and 12 months, blood and histological parameters were monitored for signs of inflammation and degradation. In the histological evaluation of the implants` environment we found degraded alloy particles, but no inflammatory reaction. Iron particles were also found within the popliteal lymph nodes on both sides. The serum blood levels of phosphorus, iron and ferritin in the long term groups were elevated. Other parameters did not show any changes. Iron-based degradable porous bone replacement implants showed a good biocompatibility in this experiment. For a clinical application, however, the rate of degradation would have to be significantly increased. Biocompatibility would then have to be re-evaluated.

scholarly journals Combined Nucleus Pulposus Augmentation and Annulus Fibrosus Repair Prevents Intervertebral Disc Degeneration After Discectomy

Neurosurgery ◽  
2019 ◽  
Vol 66 (Supplement_1) ◽  
Author(s):  
Sertac Kirnaz ◽  
Stephen Sloan ◽  
Christoph Wipplinger ◽  
Franziska Anna Schmidt ◽  
Roger Hartl ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Annulus Fibrosus ◽  
Combined Treatment ◽  
Disc Height ◽  
Histological Evaluation ◽  
Sheep Model ◽  
Mri Scans ◽  
The Individual

Abstract INTRODUCTION The objective of the current study is to assess the efficacy of combined annulus fibrosus (AF) using a high-density collagen (HDC) gel and nucleus pulposus (NP) repair using a hyaluronic acid (HA) gel in an in Vivo sheep model. METHODS We performed an anterolateral, retroperitoneal prepsoas approach to access the IVDs L1-6 in a total of 8 skeletally mature Finn sheep. IVDs were randomized into 5 groups: (1) intact, (2) injured via 3 × 10 mm box annulotomy and removal of 200 mg of NP, (3) injury and HDC gel patch for AF repair, (4) injury and injection of a HA gel into the NP, and (5) injury and HDC AF repair and NP HA replacement. At 6 wk postoperatively, sheep were sacrificed and underwent postmortem 3T-MRI scans as well as gross anatomical and histological evaluation. Disc height index (DHI) analysis and Pfirrmann grading (PG) were performed on each segment using MR images. RESULTS Intact control discs were not degenerated and had an average PG of 1 while injured, and untreated discs had a significant degeneration with an average PG of 3. Discs receiving the combined injection and collagen AF patch individually showed fewer signs of degeneration than injured alone, and the combined treatment resulted in the least amount of degeneration with PG not significantly different from the intact controls. DHI confirmed the trends seen in the PG, where injured discs lost 20% of the intact disc height, the individual NP and AF repairs restored 5% to 10% of intact disc height, and the combined repairs preserved 90% of the intact disc height. CONCLUSION PG and DHI results demonstrate that individual NP and AF repairs are able to prevent disc degeneration better than no treatment at all; however, the greatest preservation of disc health was seen with combined AF and NP repairs.

Hybrid Chitosan Membranes Tested in Sheep for Guided Tissue Regeneration

2007 ◽  
Vol 361-363 ◽  
pp. 1265-1268 ◽  
Author(s):  
P.P. Cortez ◽  
Yuki Shirosaki ◽  
C.M. Botelho ◽  
M.J. Simões ◽  
F. Gartner ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Tissue Regeneration ◽  
Chitosan Solution ◽  
Clinical Applications ◽  
Histological Evaluation ◽  
Sheep Model ◽  
The Third ◽  
Chitosan Membranes

Previous in vitro studies confirmed an improved cytocompatibility of chitosan-silicate hybrid membranes over chitosan membranes. The main goal of this study was to assess the in vivo histocompatibility of both membranes through subcutaneous implantations at different time periods, 1 week, 1, 2 and 3 months, using a sheep model. Chitosan membranes elicited an exuberant inflammatory response and were consequently rejected. The hybrid chitosan membranes were not rejected and the degree of inflammatory response decreased gradually until the third month of implantation. Histological evaluation also showed that these membranes can be resorbed in vivo. This study demonstrates that the incorporation of silicate into the chitosan solution improves its histocompatibility, indicating that the hybrid chitosan-silicate membranes are suitable candidates to be used in clinical applications.

scholarly journals Evaluation of hydroxyapatite ceramic vertebral spacers with different porosities and their binding capability to the vertebral body: an experimental study in sheep

2007 ◽  
Vol 6 (5) ◽  
pp. 431-437 ◽  
Author(s):  
Manabu Ito ◽  
Yoshihisa Kotani ◽  
Yoshihiro Hojo ◽  
Kuniyoshi Abumi ◽  
Tsuyoshi Kadosawa ◽  
...  
Keyword(s):  
Bone Ingrowth ◽  
Histological Study ◽  
Biomechanical Testing ◽  
Histological Evaluation ◽  
Micro Computed Tomography ◽  
Hydroxyapatite Ceramic ◽  
Sheep Model ◽  
Spinal Reconstruction ◽  
Computed Tomography Scans ◽  
The Ideal

Object The aim of this study was to evaluate the degree of bone ingrowth and bonding stiffness at the surface of hydroxyapatite ceramic (HAC) spacers with different porosities in an animal model and to discuss the ideal porous characteristics of these spacers for anterior spinal reconstruction. Methods Twenty-one adult sheep (age 1–2 years, mean weight 70 kg) were used in this experiment. Surgery consisted of anterior lumbar interbody fusion at L2–3 and L4–5, insertion of an HAC spacer (10 × 13 × 24 mm) with three different porosities (0, 3, and 15%), and single-rod anterior instrumentation. At 4 and 6 months postoperatively, the lumbar spines were harvested. Bonding conditions at the bone–HAC spacer interface were evaluated using neuroimages and biomechanically. A histological evaluation was also conducted to examine the state of bone ingrowth at the surface of the HAC spacer. Biomechanical testing showed that the bonding strength of HAC at 6 months postoperatively was 0.047 MPa in 0% porosity spacers, 0.39 MPa in 3%, and 0.49 MPa in 15% porosity spacers. The histological study showed that there was a soft-tissue layer at the surface of the HAC spacer with 0% porosity. Direct bonding was observed between bone and spacers with 3 or 15% porosity. Micro–computed tomography scans showed direct bonding between the bone and HAC with 3 or 15% porosity. No direct bonding was observed in HAC with 0% porosity. Conclusions Dense (0%) HAC anterior vertebral spacers did not achieve direct bonding to the bone in the sheep model. The HAC vertebral spacers with 3 or 15% porosity showed proof of direct bonding to the bone at 6 months postoperatively. The higher porosity HAC spacer showed better bonding stiffness to the bone.

Histological evaluation of the effects of 5-fluorouracil on partially divided flexor tendon injuries in rabbits

2016 ◽  
Vol 40 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Shkelzen B. Duci ◽  
Hysni M. Arifi ◽  
Hasan R. Ahmeti ◽  
Suzana Manxhuka-Kerliu ◽  
Agon Y . Mekaj ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Tendon Injuries ◽  
Histological Evaluation ◽  
Flexor Tendon Injuries

scholarly journals Serial superficial digital flexor tendon biopsies for diagnosing and monitoring collagenase-induced tendonitis in horses

2013 ◽  
Vol 33 (6) ◽  
pp. 710-718 ◽  
Author(s):  
José C. de Lacerda Neto ◽  
Juliana M.R. de Freitas ◽  
Franco M. Poggiani ◽  
Deborah P.M. Dias ◽  
Kamila Gravena ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Clinical Signs ◽  
Histological Evaluation ◽  
Tissue Samples ◽  
Biopsy Technique ◽  
Superficial Digital Flexor Tendon ◽  
The Us ◽  
Before And After ◽  
Reliable Parameter ◽  
The Right

The purpose of this investigation was to demonstrate the feasibility of a biopsy technique by performing serial evaluations of tissue samples of the forelimb superficial digital flexor tendon (SDFT) in healthy horses and in horses subjected to superficial digital flexor tendonitis induction. Eight adult horses were evaluated in two different phases (P), control (P1) and tendonitis-induced (P2). At P1, the horses were subjected to five SDFT biopsies of the left forelimb, with 24 hours (h) of interval. Clinical and ultrasonographic (US) examinations were performed immediately before the tendonitis induction, 24 and 48 h after the procedure. The biopsied tendon tissues were analyzed through histology. P2 evaluations were carried out three months later, when the same horses were subjected to tendonitis induction by injection of bacterial collagenase into the right forelimb SDFT. P2 clinical and US evaluations, and SDFT biopsies were performed before, and after injury induction at the following time intervals: after 24, 48, 72 and 96 h, and after 15, 30, 60, 90, 120 and 150 days. The biopsy technique has proven to be easy and quick to perform and yielded good tendon samples for histological evaluation. At P1 the horses did not show signs of localised inflammation, pain or lameness, neither SDFT US alterations after biopsies, showing that the biopsy procedure per se did not risk tendon integrity. Therefore, this procedure is feasible for routine tendon histological evaluations. The P2 findings demonstrate a relation between the US and histology evaluations concerning induced tendonitis evolution. However, the clinical signs of tendonitis poorly reflected the microscopic tissue condition, indicating that clinical presentation is not a reliable parameter for monitoring injury development. The presented method of biopsying SDFT tissue in horses enables the serial collection of material for histological analysis causing no clinical signs and tendon damage seen by US images. Therefore, this technique allows tendonitis to be monitored and can be considered an excellent tool in protocols for evaluating SDFT injury.

scholarly journals Arthroscopic Tenotomy of the Long Head of the Biceps Tendon and Section of the Anterior Joint Capsule Produce Moderate Osteoarthritic Changes in an Experimental Sheep Model

2021 ◽  
Vol 18 (14) ◽  
pp. 7471
Author(s):  
Umile Giuseppe Longo ◽  
Francisco Forriol ◽  
Vincenzo Candela ◽  
Salvatore Maria Tecce ◽  
Sergio De Salvatore ◽  
...  
Keyword(s):  
Shoulder Joint ◽  
Glenohumeral Joint ◽  
Biceps Tendon ◽  
Joint Capsule ◽  
Quantitative Mri ◽  
Degenerative Changes ◽  
Histological Evaluation ◽  
Sheep Model ◽  
Long Head ◽  
Development And Evolution

Osteoarthritis (OA) of the glenohumeral (GH) joint is a common cause of shoulder pain, resulting in considerable invalidity. Unfortunately, the study of its pathogenesis is challenging. Models of OA are necessary to identify specific targets for therapy and to be able to interfere with the development and evolution of OA. This study aims to assess the effect of an arthroscopic tenotomy of the long head of the biceps tendon (LHBT) and section of the anterior glenohumeral joint capsule on the ovine glenohumeral joint. In addition, the authors aim to validate and evaluate the reliability of a modified semi-quantitative MRI score to assess joint degeneration in a sheep’s shoulder. Eight skeletally mature sheep received an arthroscopic tenotomy of the LHBT and section of the anterior joint capsule and were euthanized four months after surgery. All animals tolerated the surgery well, and no complication was recorded for six weeks. Moderate degenerative changes to the ovine shoulder joint were found on MRI and histological evaluation. The arthroscopic tenotomy of the LHBT and the anterior glenohumeral joint capsule section caused moderate degenerative changes to the ovine shoulder joint.

A long-term serial histological evaluation of the patellar tendon in humans after harvesting its central third

2005 ◽  
Vol 13 (5) ◽  
pp. 398-404 ◽  
Author(s):  
Michael Svensson ◽  
Jüri Kartus ◽  
Lars Rostgård Christensen ◽  
Tomas Movin ◽  
Nikos Papadogiannakis ◽  
...  
Keyword(s):  
Patellar Tendon ◽  
Histological Evaluation

Do different tendons exhibit the same response following chronic exposure to statins?

2017 ◽  
Vol 95 (4) ◽  
pp. 333-339
Author(s):  
L.P. Oliveira ◽  
C.P. Vieira ◽  
P.P. Marques ◽  
E.R. Pimentel
Keyword(s):  
Extracellular Matrix ◽  
Statin Therapy ◽  
Patellar Tendon ◽  
Chronic Exposure ◽  
Wistar Rats ◽  
Flexor Tendon ◽  
Tendon Injuries ◽  
Collagen I ◽  
The Past ◽  
And Control

Over the past few years, a number of cases of tendon injuries associated with statin therapy have been reported. In this study, we assessed whether statins can affect the extracellular matrix (ECM) of the deep digital flexor tendon (DDFT) and patellar tendon (PT). Wistar rats were assigned to groups treated with atorvastatin (A20, A80), treated with simvastatin (S20, S80), and control. Zymography, Western blotting for collagen I, non-collagenous proteins (NCP), glycosaminoglycans (GAGs), and hydroxyproline quantifications were performed. DDFT findings: NCP were increased in A20 and A80; higher concentration of hydroxyproline was found in S80; levels of GAGs was increased in all statin-treated groups; collagen I was increased in S80 and pro-MMP-2 activity was reduced in A80, S20, and S80. PT findings: NCP were reduced in A20, A80, and S80; GAGs was reduced in A80 and S20; collagen I was increased in A20 and pro-MMP-2 activity was reduced in the S20. Both the statins provoked marked changes in both tendons. All these changes may make the tendons more prone to microdamage and ruptures. Therefore, a better understanding of the behavior of the tendon ECM components under statin therapy may provide important insights into the mechanisms behind statin-induced tendon injuries.

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