Quantification of phosphatidylethanol 16:0/18:1, 18:1/18:1, and 16:0/16:0 in venous blood and venous and capillary dried blood spots from patients in alcohol withdrawal and control volunteers

To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%;P=.008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%;P<.001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.

Download Full-text

A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots

Parasites & Vectors ◽

10.1186/s13071-015-0889-z ◽

2015 ◽

Vol 8 (1) ◽

Cited By ~ 15

Author(s):

Jansen Fernandes Medeiros ◽

Tatiana Amaral Pires Almeida ◽

Lucyane Bastos Tavares Silva ◽

Jose Miguel Rubio ◽

James Lee Crainey ◽

...

Keyword(s):

Field Trial ◽

Venous Blood ◽

Dried Blood Spots ◽

Blood Samples ◽

Blood Spots

Download Full-text

An Assessment of Dried Blood-Spot Technology for Identifying Iron Deficiency

Blood ◽

10.1182/blood.v92.5.1807 ◽

1998 ◽

Vol 92 (5) ◽

pp. 1807-1813 ◽

Cited By ~ 38

Author(s):

James D. Cook ◽

Carol H. Flowers ◽

Barry S. Skikne

Keyword(s):

Iron Deficiency ◽

Transferrin Receptor ◽

Venous Blood ◽

Dried Blood Spots ◽

Epidemiologic Studies ◽

Capillary Blood ◽

Deficiency Anemia ◽

Blood Samples ◽

Blood Spots ◽

American Society

Abstract The present study was undertaken to assess the feasibility of using ferritin and transferrin receptor measurements on dried capillary blood spots to identify iron deficiency (ID) in public health surveys. Measurements on serum and blood spots prepared from venous blood were performed in 71 healthy subjects, 41 of whom were iron-replete and 30 who had ID, either without (n = 20) or with (n = 10) anemia. Parallel measurements were performed on hemolyzed whole blood and washed hemolyzed red blood cells to assess the erythrocyte contribution of ferritin and transferrin receptor to dried blood samples. The concentration of ferritin in dried blood samples was threefold higher than serum assays due to the release of ferritin from hemolyzed erythrocytes, which diminished the usefulness of ferritin measurements for detecting ID. On the other hand, there was negligible erythrocyte contribution to the measurement of transferrin receptor in dried blood spots. The most sensitive parameter in dried blood spots was the ratio of receptor/ferritin, which was suitable for identifying iron-deficiency anemia (IDA), but less reliable than serum assays for detecting milder ID without anemia. We conclude that tandem measurements of serum ferritin and transferrin receptor in dried blood spots can be used to facilitate the identification of IDA in epidemiologic studies. © 1998 by The American Society of Hematology.

Download Full-text

Value of Phenylalanine in Amniotic Fluid Detected by MS/MS for Screening of ma ternal PKU in the Second-Trimester

10.21203/rs.3.rs-42259/v1 ◽

2020 ◽

Author(s):

yanyun wang ◽

yun Sun ◽

yu-guo Wang ◽

tao Jiang

Keyword(s):

Amniotic Fluid ◽

Reference Values ◽

Venous Blood ◽

Dried Blood Spots ◽

Unknown Etiology ◽

Case Group ◽

Multiple Gestation ◽

Second Trimester ◽

Maternal Phenylketonuria ◽

Blood Spots

Abstract Background: Phenylketonuria is the most common inherited metabolism disease in China. This study aimed to identify a new, sensitive, simple, and easy method of screening maternal Phenylketonuria in the fetal stage.Methods: Samples of amniotic fluid, dried blood spots, and blood collected on the same day were obtained from women in the second-trimester (16–28 weeks). Women had to meet the following criteria: (i) isolated high risk of quadruple marker screening or cell-free DNA screening, (ii) women who gave birth to at least one child with intellectual disability of unknown etiology, and (iii) women who gave birth to a child who died young from unknown etiology. The exclusion criteria were as follows: clinically evident chorioamnionitis, multiple gestation, and laboratory signs of infection in the amniotic fluid sample. Phenylalanine levels were measured using tandem mass spectrometry. Women who met criterion (i) served as controls whose results were used to set reference values of Phenylalanine in amniotic fluid. Women who met criteria (ii) and (iii) served as the case group whose results were used to check for maternal Phenylketonuria. The Spearman rank correlation test was used to analyze the correlation of Phenylalanine in amniotic fluid and in venous blood. Results: We analyzed 365 samples of amniotic fluid. Among them, 345 were included in the control group, with reference values of Phenylalanine in amniotic fluid of 10.79–48.47 µmol/L. Twenty cases were included in the case group. One woman in the case group was diagnosed with hyperphenylalaninemia whose fetus was diagnosed with maternal Phenylketonuria by comprehensive analysis of Phenylalanine in dried blood spots and in amniotic fluid, head circumference in fetal ultrasound, and a previous history of adverse pregnancy. The correlation between Phenylalanine concentrations in venous blood and in amniotic fluid was weak.Conclusions: Phenylalanine can be readily measured in amniotic fluid. Relative to controls, amniotic fluid levels of Phenylalanine was increased in maternal Phenylketonuria. These results can support the use of Phenylalanine as a screening tool for maternal Phenylketonuria. The significance of these change requires further study.

Download Full-text

Differences of Phenylalanine Concentrations in Dried Blood Spots and in Plasma: Erythrocytes as a Neglected Component for This Observation

Metabolites ◽

10.3390/metabo11100680 ◽

2021 ◽

Vol 11 (10) ◽

pp. 680

Author(s):

Dorothea Haas ◽

Jana Hauke ◽

Kathrin V. Schwarz ◽

Lucia Consalvi ◽

Friedrich K. Trefz ◽

...

Keyword(s):

Mass Spectrometry ◽

Venous Blood ◽

Plasma Concentrations ◽

Dried Blood Spots ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Tandem Mass ◽

Blood Samples ◽

Blood Spots ◽

Edta Plasma

Monitoring phenylalanine (Phe) concentrations is critical for the management of phenylketonuria (PKU). This can be done in dried blood spots (DBS) or in EDTA plasma derived from capillary or venous blood. Different techniques are used to measure Phe, the most common being flow-injection analysis tandem mass spectrometry (FIA-MS-MS) and ion exchange chromatography (IEC). Significant differences have been reported between Phe concentrations in various sample types measured by different techniques, the cause of which is not yet understood. We measured Phe concentrations in 240 venous blood samples from 199 patients with hyperphenylalaninemia in dried blood spots, EDTA plasma and erythrocytes by FIA-MS-MS and IEC. Phe concentrations were significantly lower in erythrocytes than in plasma leading to about 19% lower Phe DBS concentrations compared with plasma independent from the method used for quantification. As most therapy recommendations for PKU patients are based on plasma concentrations reliable conversion of DBS into plasma concentrations is necessary. Variances of Phe concentrations in plasma and DBS are not linear but increases with higher concentrations indicating heteroscedasticity. We therefore suggest the slope of the 75th percentile from quantile regression as a correction factor.

Download Full-text

Simultaneous quantification by double rocket immunoelectrophoresis of apolipoproteins A-I and B in blood spotted on filter paper.

Clinical Chemistry ◽

10.1093/clinchem/34.12.2452 ◽

1988 ◽

Vol 34 (12) ◽

pp. 2452-2455 ◽

Cited By ~ 5

Author(s):

S Micic ◽

J Arends ◽

B Nørgaard-Pedersen ◽

K Christoffersen ◽

G E Andersen

Keyword(s):

Familial Hypercholesterolemia ◽

Filter Paper ◽

Low Density Lipoprotein ◽

Venous Blood ◽

Sodium Dodecyl ◽

Density Lipoprotein ◽

Dried Blood Spots ◽

Apo B ◽

Blood Spots ◽

Simultaneous Quantification

Abstract We describe double rocket immunoelectrophoresis for simultaneous quantification of apolipoprotein A-I (apo A-I) and B (apo B) in blood on filter paper. The apolipoproteins from blood spots on filter paper were eluted with detergents (sodium dodecyl sulfate and Triton X-100). The eluates were subjected to electrophoresis on agarose gel containing antisera against both apolipoproteins. Within- and between-assay CVs for apo B/A-I ratios were less than 5.5% and 7.2%, respectively. The apo B/A-I ratio was influenced by length and temperature of storage. In results for 121 venous blood samples, the apo B/A-I ratios in dried blood spots correlated well with those in serum (r = 0.92) and correlated somewhat with the ratios for low-density lipoprotein/high-density lipoprotein cholesterol in serum (r = 0.87). Of these specimens, 68 were from patients with known familial hypercholesterolemia, all of whom had an apo B/A-I ratio greater than 0.90. We think this method will be of value for detecting familial hypercholesterolemia and possibly familial hyperapobeta- and hypoalphalipoproteinemia.

Download Full-text

Malaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.14-0614 ◽

2015 ◽

Vol 92 (3) ◽

pp. 573-577 ◽

Cited By ~ 17

Author(s):

Lydie Canier ◽

Nguon Chea ◽

Malen Ken ◽

Philippe Bosman ◽

Nimol Khim ◽

...

Keyword(s):

Venous Blood ◽

Dried Blood Spots ◽

Pcr Detection ◽

Blood Samples ◽

Low Transmission ◽

Blood Spots

Download Full-text

Reply to Verougstraete et al. Comment on HbA 1c determination from HemaSpot blood collection devices: comparison of home‐prepared dried blood spots with standard venous blood analysis

Diabetic Medicine ◽

10.1111/dme.14296 ◽

2020 ◽

Vol 37 (9) ◽

pp. 1614-1615 ◽

Cited By ~ 1

Author(s):

J. M. Hall ◽

C. F. Fowler ◽

F. Barrett ◽

R. W. Humphry ◽

S. M. MacRury

Keyword(s):

Venous Blood ◽

Dried Blood Spots ◽

Blood Analysis ◽

Blood Collection ◽

Blood Spots

Download Full-text

Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research

PLoS ONE ◽

10.1371/journal.pone.0255237 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0255237

Author(s):

Arline T. Geronimus ◽

John Bound ◽

Colter Mitchell ◽

Aresha Martinez-Cardoso ◽

Linnea Evans ◽

...

Keyword(s):

Telomere Length ◽

Mononuclear Cells ◽

Venous Blood ◽

Dried Blood Spots ◽

Social Characteristics ◽

Parameter Estimates ◽

Fresh Blood ◽

Critical Question ◽

Blood Spots ◽

Specimen Type

Background Telomere length (TL) in peripheral blood mononuclear cells (PBMC) from fresh venous blood is increasingly used to estimate molecular impacts of accumulated social adversity on population health. Sometimes, TL extracted from saliva or dried blood spots (DBS) are substituted as less invasive and more scalable specimen collection methods; yet, are they interchangeable with fresh blood? Studies find TL is correlated across tissues, but have not addressed the critical question for social epidemiological applications: Do different specimen types show the same association between TL and social constructs? Methods We integrate expertise in social epidemiology, molecular biology, and the statistical impact of measurement error on parameter estimates. Recruiting a diverse sample of 132 Metro-Detroit women, we measure TL for each woman from fresh blood PBMC, DBS, and saliva. Using regression methods, we estimate associations between social characteristics and TL, comparing estimates across specimen types for each woman. Results Associations between TL and social characteristics vary by specimen type collected from the same woman, sometimes qualitatively altering estimates of the magnitude or direction of a theorized relationship. Being Black is associated with shorter TL in PBMC, but longer TL in saliva or DBS. Education is positively associated with TL in fresh blood, but negatively associated with TL using DBS. Conclusion Findings raise concerns about the use of TL measures derived from different tissues in social epidemiological research. Investigators need to consider the possibility that associations between social variables and TL may be systematically related to specimen type, rather than be valid indicators of socially-patterned biopsychosocial processes.

Download Full-text