Integrated silica membrane–based nucleic acid purification, amplification, and visualization platform for low-cost, rapid detection of foodborne pathogens

Reducing the risk of Salmonella contamination in pet food is critical for both companion animals and humans, and its importance is reflected by the substantial increase in the demand for pathogen testing. Accurate and rapid detection of foodborne pathogens improves food safety, protects the public health, and benefits food producers by assuring product quality while facilitating product release in a timely manner. Traditional culture-based methods for Salmonella screening are laborious and can take 5 to 7 days to obtain definitive results. In this study, we developed two methods for the detection of low levels of Salmonella in pet food using real-time PCR: (i) detection of Salmonella in 25 g of dried pet food in less than 14 h with an automated magnetic bead–based nucleic acid extraction method and (ii) detection of Salmonella in 375 g of composite dry pet food matrix in less than 24 h with a manual centrifugation-based nucleic acid preparation method. Both methods included a preclarification step using a novel protocol that removes food matrix–associated debris and PCR inhibitors and improves the sensitivity of detection. Validation studies revealed no significant differences between the two real-time PCR methods and the standard U.S. Food and Drug Administration Bacteriological Analytical Manual (chapter 5) culture confirmation method.

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Assessment Criteria and Approaches for Rapid Detection Methods To Be Used in the Food Industry

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-138 ◽

2014 ◽

Vol 77 (4) ◽

pp. 670-690 ◽

Cited By ~ 11

Author(s):

MARTIN WIEDMANN ◽

SIYUN WANG ◽

LAURIE POST ◽

KENDRA NIGHTINGALE

Keyword(s):

Nucleic Acid ◽

Foodborne Pathogens ◽

Rapid Detection ◽

Food Industry ◽

Pathogen Detection ◽

Assessment Criteria ◽

Detection Methods ◽

Advantages And Disadvantages ◽

Specific Focus

The number of commercially available kits and methods for rapid detection of foodborne pathogens continues to increase at a considerable pace, and the diversity of methods and assay formats is reaching a point where it is very difficult even for experts to weigh the advantages and disadvantages of different methods and to decide which methods to choose for a certain testing need. Although a number of documents outline quantitative criteria that can be used to evaluate different detection methods (e.g., exclusivity and inclusivity), a diversity of criteria is typically used by industry to select specific methods that are used for pathogen detection. This article is intended to provide an overall outline of criteria that the food industry can use to evaluate new rapid detection methods, with a specific focus on nucleic acid–based detection methods.

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A glucose meter interface for point-of-care gene circuit-based diagnostics

Nature Communications ◽

10.1038/s41467-020-20639-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Evan Amalfitano ◽

Margot Karlikow ◽

Masoud Norouzi ◽

Katariina Jaenes ◽

Seray Cicek ◽

...

Keyword(s):

Nucleic Acid ◽

Synthetic Biology ◽

Molecular Diagnostics ◽

Point Of Care ◽

Low Cost ◽

Gene Circuit ◽

Reporter Systems ◽

Glucose Meter ◽

Pandemic Response ◽

Glucose Output

AbstractRecent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.

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Sunflower-Like Nanostructure with Built-In Hotspots for Alpha-Fetoprotein Detection

Molecules ◽

10.3390/molecules26041197 ◽

2021 ◽

Vol 26 (4) ◽

pp. 1197

Author(s):

Xiaoyu Zhao ◽

Aonan Zhu ◽

Yaxin Wang ◽

Yongjun Zhang ◽

Xiaolong Zhang

Keyword(s):

Rapid Detection ◽

Clinical Medicine ◽

Electromagnetic Coupling ◽

Low Cost ◽

Surface Enhanced Raman Spectroscopy ◽

Alpha Fetoprotein ◽

Low Concentrations ◽

Surface Enhanced Raman ◽

Active Substrate ◽

Array Structures

In the present study, a sunflower-like nanostructure array composed of a series of synaptic nanoparticles and nanospheres was manufactured through an efficient and low-cost colloidal lithography technique. The primary electromagnetic field contribution generated by the synaptic nanoparticles of the surface array structures was also determined by a finite-difference time-domain software to simulate the hotspots. This structure exhibited high repeatability and excellent sensitivity; hence, it was used as a surface-enhanced Raman spectroscopy (SERS) active substrate to achieve a rapid detection of ultra-low concentrations of Alpha-fetoprotein (AFP). This study demonstrates the design of a plasmonic structure with strong electromagnetic coupling, which can be used for the rapid detection of AFP concentration in clinical medicine.

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An impedance immunosensor based on low-cost microelectrodes and specific monoclonal antibodies for rapid detection of avian influenza virus H5N1 in chicken swabs

Biosensors and Bioelectronics ◽

10.1016/j.bios.2014.09.037 ◽

2015 ◽

Vol 67 ◽

pp. 546-552 ◽

Cited By ~ 51

Author(s):

Jianhan Lin ◽

Ronghui Wang ◽

Peirong Jiao ◽

Yuntao Li ◽

Yanbin Li ◽

...

Keyword(s):

Influenza Virus ◽

Monoclonal Antibodies ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Rapid Detection ◽

Low Cost ◽

Virus H5n1 ◽

Avian Influenza Virus H5n1 ◽

Impedance Immunosensor

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Biosensor-based Rapid Detection for Harmful Foodborne Pathogens

2020 7th International Conference on Biomedical and Bioinformatics Engineering ◽

10.1145/3444884.3444906 ◽

2020 ◽

Author(s):

Peitong Xu

Keyword(s):

Foodborne Pathogens ◽

Rapid Detection

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Application of nanomaterials in microbial-cell biosensor constructions

Chemical Papers ◽

10.2478/s11696-014-0602-2 ◽

2015 ◽

Vol 69 (1) ◽

Cited By ~ 9

Author(s):

Jana Šefčovičová ◽

Jan Tkac

Keyword(s):

Carbon Nanotubes ◽

Gold Nanoparticles ◽

Rapid Detection ◽

Food Industry ◽

Low Cost ◽

Microbial Cell ◽

Direct Connection ◽

Qualitative Detection ◽

Analytical Tools ◽

Cell Biosensor

AbstractMicrobial cell biosensors, where cells are in direct connection with a transducer enabling quantitative and qualitative detection of an analyte, are very promising analytical tools applied mainly for assays in the environmental field, food industry or biomedicine. Microbial cell biosensors are an excellent alternative to conventional analytical methods due to their specificity, rapid detection and low cost of analysis. Nowadays, nanomaterials are often used in the construction of biosensors to improve their sensitivity and stability. In this review, the combination of microbial and other individual cells with different nanomaterials (carbon nanotubes, graphene, gold nanoparticles, etc.) for the construction of biosensors is described and their applications are provided as well.

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