Assessment Criteria and Approaches for Rapid Detection Methods To Be Used in the Food Industry

2014 ◽  
Vol 77 (4) ◽  
pp. 670-690 ◽  
Author(s):  
MARTIN WIEDMANN ◽  
SIYUN WANG ◽  
LAURIE POST ◽  
KENDRA NIGHTINGALE
Keyword(s):  
Nucleic Acid ◽  
Foodborne Pathogens ◽  
Rapid Detection ◽  
Food Industry ◽  
Pathogen Detection ◽  
Assessment Criteria ◽  
Detection Methods ◽  
Advantages And Disadvantages ◽  
Specific Focus

The number of commercially available kits and methods for rapid detection of foodborne pathogens continues to increase at a considerable pace, and the diversity of methods and assay formats is reaching a point where it is very difficult even for experts to weigh the advantages and disadvantages of different methods and to decide which methods to choose for a certain testing need. Although a number of documents outline quantitative criteria that can be used to evaluate different detection methods (e.g., exclusivity and inclusivity), a diversity of criteria is typically used by industry to select specific methods that are used for pathogen detection. This article is intended to provide an overall outline of criteria that the food industry can use to evaluate new rapid detection methods, with a specific focus on nucleic acid–based detection methods.

Real-Time Nucleic Acid–Based Detection Methods for Pathogenic Bacteria in Food

2004 ◽  
Vol 67 (4) ◽  
pp. 823-832 ◽  
Author(s):  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Pathogen Detection ◽  
Food Microbiology ◽  
Detection Methods ◽  
Target Sequence ◽  
Bacterial Enumeration

Quality assurance in the food industry in recent years has involved the acceptance and implementation of a variety of nucleic acid–based methods for rapid and sensitive detection of food-associated pathogenic bacteria. Techniques such as polymerase chain reaction have greatly expedited the process of pathogen detection and have in some cases replaced traditional methods for bacterial enumeration in food. Conventional PCR, albeit sensitive and specific under optimized conditions, obligates the user to employ agarose gel electrophoresis as the means for endpoint analysis following sample processing. For the last few years, a variety of real-time PCR chemistries and detection instruments have appeared on the market, and many of these lend themselves to applications in food microbiology. These approaches afford a user the ability to amplify DNA or RNA, as well as detect and confirm target sequence identity in a closed-tube format with the use of a variety of fluorophores, labeled probes, or both, without the need to run gels. Such real-time chemistries also offer greater sensitivity than traditional gel visualization and can be semiquantitative and multiplexed depending on the specific experimental objectives. This review emphasizes the current systems available for real-time PCR–based pathogen detection, the basic mechanisms and requirements for each, and the prospects for development over the next few years in the food industry.

scholarly journals Rapid detection methods of microbial pathogens in foods – a short survey

2021 ◽  
Vol 8 (1) ◽  
pp. 5-22
Author(s):  
Barbora Vidová ◽  
Andrej Godány ◽  
Ernest Šturdík
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Pathogen Detection ◽  
Microbial Pathogens ◽  
Detection Methods ◽  
Laboratory Methods ◽  
Microbial Pathogen ◽  
Short Survey ◽  
Wide Range ◽  
The World

During harvesting, processing and handling operations foods may become contaminated with a wide range of microorganisms. This paper is presented as a short survey of recent used laboratory methods for foods microbial pathogen detection, briefly summarizing rapid, specific and sensitive methods useful for foods testing based on immunochemical and nucleic acid technologies. As the world becomes more concerned with safe foods, the demand for rapid detecting will only increase.

scholarly journals Prospects for rapid bioluminescent detection methods in the food industry – a review

2011 ◽  
Vol 23 (No. 3) ◽  
pp. 85-92 ◽  
Author(s):  
P. Dostálek ◽  
T. Brányik
Keyword(s):  
Historical Development ◽  
Food Industry ◽  
Food Samples ◽  
Detection Methods ◽  
Rapid Methods ◽  
Detection Techniques ◽  
Atp Assay ◽  
Advantages And Disadvantages ◽  
Bacterial Bioluminescence ◽  
Fluorescent Method

This review surveys rapid bioluminescent detection techniques applied in food industry and discusses the historical development of the rapid methods. These techniques are divided into two groups: methods based on bioluminescent adenosine triphosphate (ATP) assay, and on bacterial bioluminescence. The advantages and disadvantages of these methods are described. The article provides the bibliography of fluorescent method applications in food samples.    

Rapid Detection of Salmonella in Pet Food: Design and Evaluation of Integrated Methods Based on Real-Time PCR Detection

2012 ◽  
Vol 75 (2) ◽  
pp. 347-352 ◽  
Author(s):  
PRIYA BALACHANDRAN ◽  
MARIA FRIBERG ◽  
V. VANLANDINGHAM ◽  
K. KOZAK ◽  
AMANDA MANOLIS ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Companion Animals ◽  
Magnetic Bead ◽  
Acid Extraction ◽  
Food Matrix ◽  
Pet Food

Reducing the risk of Salmonella contamination in pet food is critical for both companion animals and humans, and its importance is reflected by the substantial increase in the demand for pathogen testing. Accurate and rapid detection of foodborne pathogens improves food safety, protects the public health, and benefits food producers by assuring product quality while facilitating product release in a timely manner. Traditional culture-based methods for Salmonella screening are laborious and can take 5 to 7 days to obtain definitive results. In this study, we developed two methods for the detection of low levels of Salmonella in pet food using real-time PCR: (i) detection of Salmonella in 25 g of dried pet food in less than 14 h with an automated magnetic bead–based nucleic acid extraction method and (ii) detection of Salmonella in 375 g of composite dry pet food matrix in less than 24 h with a manual centrifugation-based nucleic acid preparation method. Both methods included a preclarification step using a novel protocol that removes food matrix–associated debris and PCR inhibitors and improves the sensitivity of detection. Validation studies revealed no significant differences between the two real-time PCR methods and the standard U.S. Food and Drug Administration Bacteriological Analytical Manual (chapter 5) culture confirmation method.

scholarly journals Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review

Biosensors ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 58
Author(s):  
Athmar A. Ali ◽  
Ammar B. Altemimi ◽  
Nawfal Alhelfi ◽  
Salam A. Ibrahim
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Rapid Detection ◽  
Food Industry ◽  
Food Products ◽  
Biological Detection ◽  
Novel Approach ◽  
Industrial Use ◽  
Biochemical Signals ◽  
Food Bacteria

The use of biosensors is considered a novel approach for the rapid detection of foodborne pathogens in food products. Biosensors, which can convert biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. The objective of this review was to present the effectiveness of various forms of sensing technologies for the detection of foodborne pathogens in food products, as well as the criteria for industrial use of this technology. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in the food industry are summarized. This review also focuses in detail on the application of the most widely used biosensor types in food safety.

Isothermal Target and Probe Amplification Assay for the Real-Time Rapid Detection of Staphylococcus aureus

2015 ◽  
Vol 78 (4) ◽  
pp. 723-727 ◽  
Author(s):  
HYEWON SHIN ◽  
MINHWAN KIM ◽  
EUNJU YOON ◽  
GYOUNGWON KANG ◽  
SEUNGYU KIM ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Rapid Detection ◽  
Food Poisoning ◽  
Detection Methods ◽  
Economic Losses ◽  
Fluorescence Detector ◽  
The Real ◽  
Robust Detection ◽  
Advantages And Disadvantages

Staphylococcus aureus, the species most commonly associated with staphylococcal food poisoning, is one of the most prevalent causes of foodborne disease in Korea and other parts of the world, with much damage inflicted to the health of individuals and economic losses estimated at $120 million. To reduce food poisoning outbreaks by implementing prevention methods, rapid detection of S. aureus in foods is essential. Various types of detection methods for S. aureus are available. Although each method has advantages and disadvantages, high levels of sensitivity and specificity are key aspects of a robust detection method. Here, we describe a novel real-time isothermal target and probe amplification (iTPA) method that allows the rapid and simultaneous amplification of target DNA (the S. aureus nuc gene) and a fluorescence resonance energy transfer–based signal probe under isothermal conditions at 61°C or detection of S. aureus in real time. The assay was able to specifically detect all 91 S. aureus strains tested without nonspecific detection of 51 non–S. aureus strains. The real-time iTPA assay detected S. aureus at an initial level of 101 CFU in overnight cultures of preenriched food samples (kiwi dressing, soybean milk, and custard cream). The advantage of this detection system is that it does not require a thermal cycler, reducing the cost of the real-time PCR and its footprint. Combined with a miniaturized fluorescence detector, this system can be developed into a simplified quantitative hand-held real-time device, which is often required. The iTPA assay was highly reliable and therefore may be used as a rapid and sensitive means of identifying S. aureus in foods.

scholarly journals Establishment and Application of Reverse Transcription Recombinase-Aided Amplification as A Rapid Detection Method for SARS-COV-2

2020 ◽  
Author(s):  
Lele Ai ◽  
Wei Liu ◽  
Fuqiang Ye ◽  
Chenxi Ding ◽  
Han Dai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection Methods ◽  
Influenza B Virus ◽  
Influenza B ◽  
The Novel ◽  
Efficient Detection ◽  
Novel Coronavirus

Abstract Background: By the end of August 2020, >23 million cases and 800,000 deaths were attributed to SARS-CoV-2 in >200 countries. The improvement of simple, rapid, and efficient detection methods is of great significance for the early detection, timely isolation, and protection of susceptible populations. This study aimed to provide an alternative method for the rapid detection of viral nucleic acid.Methods: This study provided a rapid nucleic acid detection method mediated by recombinant enzyme based on the novel coronavirus (SARS-CoV-2). Primers and probes were designed based on the N gene sequence of coronavirus. The method was performed at 39 °C, the detection time was short (<20 min), and the detection limit was up to 101 copies/mL.Results: The primer-probe did not show any cross-reaction with adenovirus, Zika virus, influenza B virus, and chikungunya virus, with good specificity. A total of 106 clinical throat swab samples were compared by reverse transcription recombinase-aided amplification (RT-RAA) and commercial reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR); the results were identical.Conclusions: The novel coronavirus RT-RAA method established in this study had high sensitivity, strong specificity, simple operation, and fast detection speed, and hence, is suitable for the rapid detection of novel coronavirus under the current epidemic situation.

scholarly journals Research Progress in Determination Methods of Formaldehyde Content in Indoor Air

2021 ◽  
Vol 2 (4) ◽  
Author(s):  
Bowei Yao ◽  
Hairui Wang ◽  
Wei Zhang ◽  
Lili Feng
Keyword(s):  
Indoor Air ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection Methods ◽  
Research Progress ◽  
Formaldehyde Content ◽  
Advantages And Disadvantages ◽  
Determination Methods ◽  
Phenol Reagent ◽  
Detection Principle

The detection methods of formaldehyde content in indoor air, including traditional laboratory detection methods (AHMT spectrophotometry, phenol reagent spectrophotometry, acetyl acetone spectrophotometry and gas chromatography) and rapid detection methods (electrochemical sensor method, photoelectric spectrophotometry, etc.), were introduced and described. This paper systematically analyzes and compares the detection principle, applicable environment medium, detection flux and the advantages and disadvantages of each detection method. The future detection methods of formaldehyde content in indoor air were prospected.

Bacteriocins, a natural weapon against bacterial contamination for greater safety and preservation of food

2020 ◽  
Vol 21 ◽  
Author(s):  
Virginia Fuochi ◽  
Rosalia Emma ◽  
Pio Maria Furneri
Keyword(s):  
Foodborne Pathogens ◽  
Food Industry ◽  
Bacterial Contamination ◽  
Chemical Compounds ◽  
Antimicrobial Activities ◽  
Food Samples ◽  
Antibacterial Peptides ◽  
Advantages And Disadvantages ◽  
Spoilage Bacteria ◽  
Natural Preservatives

: Nowadays, consumers have become increasingly attentive to human health and the use of more natural products. Consequently, the demand for natural preservatives in the food industry is more frequent. This has led to an intense research to discover new antimicrobial compounds of natural origin which could effectively fight foodborne pathogens. This research aims to safeguard the health of consumers and, above all, to avoid potentially harmful chemical compounds. Lactobacillus is a bacterial genus belonging to the Lactic Acid Bacteria and many strains are defined GRAS, generally recognized as safe. These strains are able to produce substances with antibacterial activity against food spoilage bacteria and contaminating pathogens: the bacteriocins. The aim of this review was to focus on this genus and their capability to produce antibacterial peptides. The review collected all the information of the last few years about bacteriocins produced by Lactobacillus strains, isolated from clinical or food samples, with remarkable antimicrobial activities useful for being exploited in the food field. In addition, the advantages and disadvantages of their use, and the possible ways of improvement for industrial application were described.

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