scholarly journals Synthesis and application of stationary phase for DNA-affinity chromatographic analysis of unmodified and antisense oligonucleotide

2021 ◽  
Author(s):  
Sylwia Studzińska ◽  
Ewelina Zawadzka ◽  
Szymon Bocian ◽  
Michał Szumski
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Affinity Chromatography ◽  
Antisense Oligonucleotide ◽  
Antisense Oligonucleotides ◽  
Stationary Phases ◽  
Exchange Chromatography ◽  
Oligonucleotide Hybridization ◽  
Immobilized Stationary Phase ◽  
Pentanedioic Acid

AbstractThe goal of the research was the synthesis and application of an oligonucleotide immobilized stationary phase for the analysis of unmodified and antisense oligonucleotides. The method for attaching these molecules to aminopropyl silica modified with pentanedioic acid was developed. Each step of the synthesis was carefully controlled with the application of spectroscopic, elemental, and chromatographic analyses. The oligonucleotide-based stationary phase was applied for the retention studies. Unmodified oligonucleotides of different complementarity to the molecule attached as a stationary phase, as well as antisense oligonucleotides, were tested. The comparative study upon complex optimization of oligonucleotide analysis in different liquid chromatography modes was performed. Results have shown that this stationary phase may be applied for oligonucleotide analysis in hydrophilic interaction liquid chromatography and ion exchange chromatography, but no unique sequence-based selectivity was obtained. Contrary results were observed for affinity chromatography, which allowed for specific separation of the complementary strands based on hydrogen bonding and stacking interactions, where the temperature was the main factor influencing the selectivity of the separation. Furthermore, the oligonucleotide-based stationary phase may be applied for comparative antisense oligonucleotide hybridization studies to a specific RNA sequence. All of the results have shown that affinity chromatography with oligonucleotide-based stationary phases is a powerful technique for the specific base recognition of polynucleotides. Graphical abstract

Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations

2016 ◽  
Vol 8 (31) ◽  
pp. 6038-6045 ◽  
Author(s):  
Yadi Wang ◽  
M. Farooq Wahab ◽  
Zachary S. Breitbach ◽  
Daniel W. Armstrong
Keyword(s):  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Stationary Phase ◽  
Mixed Mode ◽  
High Efficiency ◽  
Stationary Phases ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Stationary phases composed of native cyclofructan 6 (CF6) and benzoic acid modified CF6 were synthesized and evaluated for hydrophilic interaction liquid chromatography (HILIC).

scholarly journals Current Trends in Protein Purification : A Review

2020 ◽  
pp. 279-310
Author(s):  
Angela Boxi ◽  
Isha Parikh ◽  
Radhika B S ◽  
Shryli K S
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Affinity Chromatography ◽  
Protein Purification ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Current Trends ◽  
Comparative Information

The present review is based on papers published between 1990 and 2020 and gives Comparative information about the most common protein purification techniques Gel-Filtration, Chromatography, Ion-Exchange Chromatography, Electrophoresis, Affinity Chromatography, and Dialysis, High-Pressure Liquid Chromatography. and their applications.

scholarly journals Comparison of Various Chromatographic Systems for Identification of Vortioxetine in Bulk Drug Substance, Human Serum, Saliva, and Urine Samples by HPLC-DAD and LC-QTOF-MS

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2483 ◽  
Author(s):  
Anna Petruczynik ◽  
Karol Wróblewski ◽  
Krzysztof Wojtanowski ◽  
Tomasz Mroczek ◽  
Dariusz Juchnowicz ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Human Serum ◽  
Psychotropic Drugs ◽  
Biological Samples ◽  
Stationary Phases ◽  
Log P ◽  
Therapeutic Drug ◽  
Organic Modifiers ◽  
Drug Determination

Background: Determination of psychotropic drugs in clinical study is significant, and the establishment of methodologies for these drugs in biological matrices is essential for patients’ safety. The search for new methods for their detection is one of the most important challenges of modern scientific research. The methods for analyzing of psychotropic drugs and their metabolites in different biological samples should be based on combining a very efficient separation technique including high-performance liquid chromatography (HPLC), with a sensitive detection method and effectively sample preparation methods. Objective: Retention, peaks symmetry and system efficiency of vortioxetine on Hydro RP, Polar RP, HILIC A (with silica stationary phase), HILIC-B (with aminopropyl stationary phase), and ACE HILIC-N (with polyhydroxy stationary phase and SCX columns were investigated. Various mobile phases containing methanol or acetonitrile as organic modifiers and different additives were also applied to obtained optimal retention, peaks shape, and systems efficiency. The best chromatographic procedure was used for simultaneous analysis of vortioxetine and its metabolites in human serum, urine and saliva samples. Methods: Analysis of vortioxetine was performed in various chromatographic systems: Reversed phase (RP) systems on alkylbonded or phenyl stationary phases, hydrophilic interaction liquid chromatography (HILIC), and ion-exchange chromatography (IEC). Based on the dependence of log k vs the concentration of the organic modifier, log kw values for vortioxetine in various chromatographic systems were determined and compared with calculated log P values. Solid phase extraction (SPE) method was applied for sample pre-treatment before HPLC analysis. HPLC-QTOF-MS method was applied for confirmation of presence of vortioxetine and some its metabolites in biological samples collected from psychiatric patient. Conclusions: Differences were observed in retention parameters with a change of the applied chromatographic system. The various properties of stationary phases resulted in differences in vortioxetine retention, systems’ efficiency, and peaks’ shape. Lipophilicity parameters were also determined using different HPLC conditions. The most optimal systems were chosen for the analysis of vortioxetine in biological samples. Both serum and urine or saliva samples collected from patients treated with vortioxetine can be used for the drug determination. For the first time, vortioxetine was detected in patient’s saliva. Obtained results indicate on possibility of application of saliva samples, which collection are non-invasive and painless, for determination and therapeutic drug monitoring in patients.

scholarly journals Separation of Inorganic Anions and Phenolic Compounds Using Tetraethylene Oxide-Bonded Stationary Phases in Capillary Liquid Chromatography

2019 ◽  
Vol 19 (1) ◽  
pp. 191
Author(s):  
Roza Linda ◽  
Mohamad Rafi ◽  
Lee Wah Lim ◽  
Toyohide Takeuchi
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Stationary Phase ◽  
Stationary Phases ◽  
Capillary Liquid Chromatography ◽  
Inorganic Anions ◽  
Tetraethylene Glycol ◽  
Modified Silica ◽  
Relative Standard ◽  
Capillary Liquid

Tetraethylene glycol monomethyl ether (TEGMM) and tetraethylene glycol (TEG) modified silica were synthesized and used in capillary liquid chromatography as stationary phases. The stationary phases were prepared by chemically bonding TEGMM or TEG on silica via reaction with 3-glycidyloxypropyltrimethoxysilane. The present stationary phases were successfully used for the separation of several inorganic anions and phenolic compounds. The TEG-modified silica stationary phase had a better selectivity and higher retention for five anions compared to TEGMM-modified silica stationary phase. The repeatability of retention time for the five anions was satisfactory on both stationary phases. By using 2 mM sodium chloride as eluent, the relative standard deviation values were in ranging from 0.68–3.21 and 2.00–2.16% for analytes in the TEG-and TEGMM-bonded stationary phase, respectively. It was found that the TEG-bonded stationary phase had hydrophilic properties due to the presence of the hydroxyl group at the end of the TEG chains.

Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5594-5599 ◽  
Author(s):  
Shunli Ji ◽  
Feifang Zhang ◽  
Shengjie Wu ◽  
Bingcheng Yang ◽  
Xinmiao Liang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Polyvinyl Alcohol ◽  
Stationary Phase ◽  
High Performance ◽  
Stationary Phases ◽  
Physical Method ◽  
Facile Preparation

A facile physical method to prepare polyvinyl alcohol coating-based silica stationary phase for HPLC was proposed.

Chiral separation and a molecular modeling study of eight azole antifungals on the cellulose tris(3,5-dichlorophenylcarbamate) chiral stationary phase

2018 ◽  
Vol 42 (16) ◽  
pp. 13421-13429 ◽  
Author(s):  
Bolin Zhu ◽  
Fan Zhao ◽  
Jia Yu ◽  
Zhaokun Wang ◽  
Yongbo Song ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Molecular Modeling ◽  
Stationary Phase ◽  
Chiral Separation ◽  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Normal Phase ◽  
Azole Antifungals ◽  
Phase Liquid

Four immobilized polysaccharide-based chiral stationary phases have been examined for their enantioselectivity on azole analytes using normal phase liquid chromatography.

scholarly journals The Development of Silica Hydride Stationary Phases for High-Performance Liquid Chromatography from Conception to Commercialization

Separations ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 27 ◽  
Author(s):  
Maria Matyska ◽  
Joseph Pesek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Surface Modification ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Silica Surface ◽  
Stationary Phases ◽  
Retention Mechanism ◽  
Silica Hydride ◽  
Synthetic Approaches

The development of a stationary phase material for high-performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization, and applications. There are several synthetic approaches available to create a silica hydride surface. Modification of the Si–H moiety on the silica surface can be accomplished through the use of a hydrosilation reaction. Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods. Further insights into the retention mechanism are provided through chromatographic measurements. The ultimate utility of any chromatographic stationary phase material is determined by its success in solving challenging analytical problems. A broad range of applications is reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases.

scholarly journals Supramolecular Chromatographic Separation of C60 and C70 Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography

2021 ◽  
Vol 22 (11) ◽  
pp. 5726
Author(s):  
Subbareddy Mekapothula ◽  
A. D. Dinga Wonanke ◽  
Matthew A. Addicoat ◽  
David J. Boocock ◽  
John D. Wallis ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Pressure Liquid Chromatography ◽  
Column Chromatography ◽  
Stationary Phases ◽  
In Silico Studies ◽  
Flash Column Chromatography ◽  
New Stationary Phase ◽  
Fullerene Mixture

A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C60 and C70 fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound C-butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C60- and C70-fullerene mixture more effectively than an RP-C18 stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography.

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