Investigation on secondary metabolite content and antioxidant activity of commercial saffron powder

2016 ◽  
Vol 242 (6) ◽  
pp. 987-993 ◽  
Author(s):  
Eleonora Urbani ◽  
Francesca Blasi ◽  
Maria Stella Simonetti ◽  
Claudia Chiesi ◽  
Lina Cossignani
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolite ◽  
Metabolite Content ◽  
Saffron Powder

scholarly journals The Influence of Seasonality on Secondary Metabolite Profiles and Neuroprotective Activities of Moss Hypnum cupressiforme Extracts: In Vitro and In Silico Study

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 123
Author(s):  
Tanja M. Lunić ◽  
Marija R. Mandić ◽  
Mariana M. Oalđe Pavlović ◽  
Aneta D. Sabovljević ◽  
Marko S. Sabovljević ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Hypnum Cupressiforme ◽  
Tnf Α ◽  
In Silico Study ◽  
Metabolite Profiles ◽  
Metabolite Content

Numerous representatives of mosses, including Hypnum cupressiforme, have been used to alleviate different inflammation-related conditions. However, the mode of action underlying this anti-inflammatory potential has been poorly understood. Moreover, the influence of seasonality on the chemical composition and biological activity of mosses is generally overlooked. This study aimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondary metabolite composition and biological activities of ethyl acetate H. cupressiforme extracts. Antioxidant activity was measured using β-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assays were carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts. Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and by docking analysis. The highest content of secondary metabolites and antioxidant activity were observed in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, and IL-6, alleviating the inflammatory potential of H2O2 and LPS in microglial and neuronal cells. Strong inhibitory effects on acetylcholinesterase and tyrosinase were observed in vitro. Docking analyses revealed high-affinity interactions of secondary metabolites present in H. cupressiforme with important enzyme residues. Altogether, these results reveal the neuroprotective potential and the significance of seasonal fluctuations on secondary metabolite content and biological activities in moss H. cupressiforme.

scholarly journals POTENSI BEBERAPA JENIS RUMPUT LAUT DI ACEH (STUDI KASUS: SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN)

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Adean Mayasri
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Seaweed Species ◽  
Dpph Method ◽  
Metabolite Content

One of the sea potentials in Aceh is seaweed. Seaweed contains secondary metabolites and antioxidants that are very well consumed because it can act as an antidote/protector from the free radicals. This study aimed to determine the secondary metabolite content and antioxidant activity of seaweed in Aceh.The seaweed that has been macerated then screened for phytochemicals and tested for antioxidant activity by using the DPPH method. The seaweed species found were Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina. The results of this study indicated that Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina seaweeds were contained by alkaloids and steroids. Flavonoid and saponins were only detected in the types of seaweed Gracillaria verrucosa and Sargassum sp. It was also found that the higher the concentration of the extract, the higher the percentage of IC50 against free radicals. The Sargassum sp. has a stable percentage of inhibition at a 2-10 ppm concentration, so it is recommended in this study to be considered cultivated in Aceh.

scholarly journals Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract

2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Properties ◽  
Linear Connection ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Control Group

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

scholarly journals Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

2021 ◽  
Vol 869 (1) ◽  
pp. 012010
Author(s):  
S Agustina ◽  
S Bella ◽  
S Karina ◽  
I Irwan ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Column Chromatography ◽  
Extraction Method ◽  
Sea Cucumber ◽  
Sea Cucumbers ◽  
Marine Chemistry ◽  
Holothuria Atra ◽  
Metabolite Content

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

scholarly journals Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba

2021 ◽  
pp. 23-32
Author(s):  
Verly Dotulong ◽  
Djuhria Wonggo ◽  
Lita A. D. Y. Montolalu
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Activities ◽  
Extraction Methods ◽  
Water Fraction ◽  
Sonneratia Alba ◽  
Dpph Method ◽  
Young Leaves

The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate  fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol  (3.43±0.25 µg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.

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