Acid Resistance Contributes to the High-Pressure Carbon Dioxide Resistance of Escherichia coli K-12

2014 ◽  
Vol 70 (1) ◽  
pp. 1-5
Author(s):  
Soichi Furukawa ◽  
Junji Shimazaki ◽  
Kazumichi Kawaharada ◽  
Tsukasa Matsuda ◽  
Hiroki Aoyagi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
High Pressure ◽  
Acid Resistance ◽  
K 12

scholarly journals Effect of high-pressure carbon dioxide processing on the inactivation of aerobic mesophilic bacteria and Escherichia coli in human milk

2017 ◽  
Vol 16 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Ana Claudia Berenhauser ◽  
Douglas Soares ◽  
Norton Komora ◽  
Juliano De Dea Lindner ◽  
Elane Schwinden Prudêncio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
High Pressure ◽  
Human Milk ◽  
Mesophilic Bacteria

scholarly journals The Small Noncoding DsrA RNA Is an Acid Resistance Regulator in Escherichia coli

2004 ◽  
Vol 186 (18) ◽  
pp. 6179-6185 ◽  
Author(s):  
Richard A. Lease ◽  
Dorie Smith ◽  
Kathleen McDonough ◽  
Marlene Belfort
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Resistance Genes ◽  
Acid Resistance ◽  
Dna Arrays ◽  
E Coli ◽  
Specific Mrnas ◽  
Steady State Levels ◽  
Control Translation ◽  
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ABSTRACT DsrA RNA is a small (87-nucleotide) regulatory RNA of Escherichia coli that acts by RNA-RNA interactions to control translation and turnover of specific mRNAs. Two targets of DsrA regulation are RpoS, the stationary-phase and stress response sigma factor (σs), and H-NS, a histone-like nucleoid protein and global transcription repressor. Genes regulated globally by RpoS and H-NS include stress response proteins and virulence factors for pathogenic E. coli. Here, by using transcription profiling via DNA arrays, we have identified genes induced by DsrA. Steady-state levels of mRNAs from many genes increased with DsrA overproduction, including multiple acid resistance genes of E. coli. Quantitative primer extension analysis verified the induction of individual acid resistance genes in the hdeAB, gadAX, and gadBC operons. E. coli K-12 strains, as well as pathogenic E. coli O157:H7, exhibited compromised acid resistance in dsrA mutants. Conversely, overproduction of DsrA from a plasmid rendered the acid-sensitive dsrA mutant extremely acid resistant. Thus, DsrA RNA plays a regulatory role in acid resistance. Whether DsrA targets acid resistance genes directly by base pairing or indirectly via perturbation of RpoS and/or H-NS is not known, but in either event, our results suggest that DsrA RNA may enhance the virulence of pathogenic E. coli.

scholarly journals Accurate flow cytometric monitoring of Escherichia coli subpopulations on solid food treated with high pressure carbon dioxide

2014 ◽  
Vol 117 (2) ◽  
pp. 440-450 ◽  
Author(s):  
S. Tamburini ◽  
P. Foladori ◽  
G. Ferrentino ◽  
S. Spilimbergo ◽  
O. Jousson
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
High Pressure ◽  
Solid Food ◽  
Flow Cytometric

scholarly journals Availability of Glutamate and Arginine during Acid Challenge Determines Cell Density-Dependent Survival Phenotype ofEscherichia coli Strains

2001 ◽  
Vol 67 (10) ◽  
pp. 4914-4918 ◽  
Author(s):  
Shenghui Cui ◽  
Jianghong Meng ◽  
Arvind A. Bhagwat
Keyword(s):  
Escherichia Coli ◽  
Cell Density ◽  
Acid Resistance ◽  
Arginine Decarboxylase ◽  
Ofescherichia Coli ◽  
Acid Sensitivity ◽  
Density Dependent ◽  
Survival Pathways ◽  
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Acid Challenge

ABSTRACT The cell density-dependent acid sensitivity phenotypes ofEscherichia coli strains K-12 and O157:H7 were examined with reference to three possible mechanisms of acid resistance. There was no evidence of any diffusible substance released from dead cells which could influence the cell density-dependent acid survival phenotype. Instead, cell density-dependent acid survival phenotype was associated with induction of glutamate- and arginine-decarboxylase acid survival pathways and concomitant availability of glutamate and arginine during acid challenge.

scholarly journals Global Analysis of Posttranscriptional Regulation by GlmY and GlmZ in Enterohemorrhagic Escherichia coli O157:H7

2015 ◽  
Vol 83 (4) ◽  
pp. 1286-1295 ◽  
Author(s):  
Charley C. Gruber ◽  
Vanessa Sperandio
Keyword(s):  
Escherichia Coli ◽  
Rna Sequencing ◽  
Posttranscriptional Regulation ◽  
Global Analysis ◽  
Acid Resistance ◽  
Enterohemorrhagic Escherichia Coli ◽  
Global Changes ◽  
Content Type ◽  
Enterocyte Effacement ◽  
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EnterohemorrhagicEscherichia coli(EHEC) is a significant human pathogen and is the cause of bloody diarrhea and hemolytic-uremic syndrome. The virulence repertoire of EHEC includes the genes within the locus of enterocyte effacement (LEE) that are largely organized in five operons,LEE1toLEE5, which encode a type III secretion system, several effectors, chaperones, and regulatory proteins. In addition, EHEC also encodes several non-LEE-encoded effectors and fimbrial operons. The virulence genes of this pathogen are under a large amount of posttranscriptional regulation. The small RNAs (sRNAs) GlmY and GlmZ activate the translation of glucosamine synthase (GlmS) inE. coliK-12, and in EHEC they destabilize the 3′ fragments of theLEE4andLEE5operons and promote translation of the non-LEE-encoded effector EspFu. We investigated the global changes of EHEC gene expression governed by GlmY and GlmZ using RNA sequencing and gene arrays. This study extends the known effects of GlmY and GlmZ regulation to show that they promote expression of the curli adhesin, repress the expression of tryptophan metabolism genes, and promote the expression of acid resistance genes and the non-LEE-encoded effector NleA. In addition, seven novel EHEC-specific sRNAs were identified using RNA sequencing, and three of them—sRNA56, sRNA103, and sRNA350—were shown to regulate urease, fimbria, and the LEE, respectively. These findings expand the knowledge of posttranscriptional regulation in EHEC.

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