Zearalenone Adsorbent Based on a Lyophilized Indigenous Bacterial Lactobacillus plantarum Strain as Feed Additive for Pigs: A Preliminary Study In Vivo

2021 ◽  
Vol 78 (5) ◽  
pp. 1807-1812
Author(s):  
María F. Vega ◽  
Susana N. Diéguez ◽  
Belén Riccio ◽  
María O. Tapia ◽  
Silvia N. González
Keyword(s):  
Lactobacillus Plantarum ◽  
Feed Additive ◽  
Preliminary Study

scholarly journals Partial Decellularization for Segmental Tracheal Scaffold Tissue Engineering: A Preliminary Study in Rabbits

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 866
Author(s):  
Luong Huu Dang ◽  
Yuan Tseng ◽  
How Tseng ◽  
Shih-Han Hung
Keyword(s):  
Mechanical Stability ◽  
Vital Staining ◽  
Potential Method ◽  
Term Survival ◽  
Long Term Survival ◽  
Cartilage Cells ◽  
Preliminary Study ◽  
Epithelial Layers

In this study, we developed a new procedure for the rapid partial decellularization of the harvested trachea. Partial decellularization was performed using a combination of detergent and sonication to completely remove the epithelial layers outside of the cartilage ring. The post-decellularized tracheal segments were assessed with vital staining, which showed that the core cartilage cells remarkably remained intact while the cells outside of the cartilage were no longer viable. The ability of the decellularized tracheal segments to evade immune rejection was evaluated through heterotopic implantation of the segments into the chest muscle of rabbits without any immunosuppressive therapy, which demonstrated no evidence of severe rejection or tissue necrosis under H&E staining, as well as the mechanical stability under stress-pressure testing. Finally, orthotopic transplantation of partially decellularized trachea with no immunosuppression treatment resulted in 2 months of survival in two rabbits and one long-term survival (2 years) in one rabbit. Through evaluations of posttransplantation histology and endoscopy, we confirmed that our partial decellularization method could be a potential method of producing low-immunogenic cartilage scaffolds with viable, functional core cartilage cells that can achieve long-term survival after in vivo transplantation.

scholarly journals Feasibility of balloon-based endobiliary radiofrequency ablation under cholangioscopy guidance in a swine model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tadahisa Inoue ◽  
Hiromu Kutsumi ◽  
Mayu Ibusuki ◽  
Masashi Yoneda
Keyword(s):  
Radiofrequency Ablation ◽  
Adverse Events ◽  
Swine Model ◽  
Biliary Strictures ◽  
Tissue Samples ◽  
Ablation Area ◽  
Mucosal Changes ◽  
The Mean ◽  
Preliminary Study

AbstractAlthough endobiliary radiofrequency ablation (RFA) has demonstrated considerable potential for the treatment of biliary strictures, conventional catheter RFA has several limitations. This study aimed to evaluate the feasibility of a novel cholangioscopy (CS)-guided balloon-based RFA procedure in vivo using a swine model. CS-guided balloon-RFA was performed under endoscopic retrograde cholangiography guidance at target temperatures of 60 ℃ or 70 ℃, which were maintained for 60 s. We evaluated the technical feasibility, adverse events, and histological effects associated with the procedure. Twelve sites were ablated in seven miniature pigs. The CS-guided balloon-RFA procedure was technically successful in all cases without any hindrance. Mucosal changes could be detected during RFA, and the ablation area was identified on CS. Necropsy was performed in four pigs on the same day as the procedure: the tissue samples showed coagulative necrosis, and the entire internal circumference of the bile duct was uniformly ablated. The mean lengths of the ablation area in the samples ablated at 60 °C and 70 °C were 20.64 and 22.18 mm, respectively, while the mean depths were 3.46 and 5.07 mm, respectively. The other three pigs were reared and euthanized and autopsied 35 days after the procedure. The site to be ablated had replaced the granulation tissue and fibrotic changes. No adverse events were observed in any case. CS-guided balloon-RFA appears to be a promising option for treating biliary strictures. This preliminary study could pave the way for the evaluation of this procedure in future human clinical trials.

scholarly journals Preliminary study on Se-enriched Lentinula edodes mycelium as a proposal of new feed additive in selenium deficiency

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233456 ◽  
Author(s):  
Bożena Muszyńska ◽  
Ewelina Szacawa ◽  
Dorota Bederska-Łojewska ◽  
Katarzyna Dudek ◽  
Bartosz Pomierny ◽  
...  
Keyword(s):  
Lentinula Edodes ◽  
Selenium Deficiency ◽  
Feed Additive ◽  
Preliminary Study

scholarly journals Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

2005 ◽  
Vol 13 (3) ◽  
pp. 702 ◽  
Author(s):  
Michal Heger ◽  
Johan F. Beek ◽  
Karin Stenback ◽  
Dirk J. Faber ◽  
Martin J.C. van Gemert ◽  
...  
Keyword(s):  
Spectral Imaging ◽  
Tissue Interactions ◽  
Preliminary Study

Preliminary study of in vivo autofluorescence of nasopharyngeal carcinoma and normal tissue

2000 ◽  
Vol 26 (5) ◽  
pp. 432-440 ◽  
Author(s):  
Jianan Y. Qu ◽  
Po Wing ◽  
Zhijian Huang ◽  
Dora Kwong ◽  
Jonathan Sham ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Normal Tissue ◽  
Preliminary Study

scholarly journals Evaluación in vivo de Lactobacillus plantarum como alternativa al uso de antibióticos en lechones

2013 ◽  
pp. 3648-3657 ◽  
Author(s):  
Henry Jurado-Gámez ◽  
Cristina Ramírez T ◽  
Javier Martínez B
Keyword(s):  
Lactobacillus Plantarum

RESUMENObjetivos. Evaluar el efecto in vivo de Lactobacillus plantarum como alternativa al uso de antibióticos en lechones. Materiales y métodos. 50 lechones fueron distribuidos en 5 tratamientos (n=10). (T0: sin probiótico; T1: con L. plantarum 1 H1; T2: con L. plantarum 1 H2; T3: con probiótico comercial; T4: sin probiótico comercial). Las cepas fueron identificadas molecularmente. Para la elaboración de los inóculos se utilizaron 10 g/L azúcar blanco; 15 g/L leche de soya; 150 g/L suero de leche; 15 g/L salvado de trigo y se analizó la viabilidad a temperatura ambiente y refrigeración. El efecto de los inóculos probióticos se evaluó en ganancia de peso, sobrevivencia y presentación de diarrea. La determinación del contenido de inmunoglobulina A se hizo por turbidimetría; colesterol total y BUN por espectofotometría. El recuento de polimorfonucleares neutrófilos mediante extendido de sangre. Resultados. Las cepas correspondieron molecularmente a Lactobacillus plantarum. La producción de los inóculos, para el caso de L. plantarum 1 H1 y L. plantarum 1 H2 se almacenaron por 21 y 12 días a temperatura de refrigeración manteniendo viabilidades altas. Los animales sometidos a los tratamientos T1 y T2 no presentaron episodios de diarrea y la mayor ganancia de peso vivo final, así como las concentraciones más altas de IgA, polimorfonucleares neutrófilos, colesterol total y BUN fueron menores en T1 y T2. Se comprobó la adherencia de L. plantarum 1 H1 y L. plantarum 1 H2 en el intestino grueso. Conclusiones. La utilización de probióticos con L. plantarum 1 mostraron un efecto positivo en la salud, sobrevivencia y ganancia de peso de los lechones tratados.

scholarly journals Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

2020 ◽  
Vol 76 (03) ◽  
pp. 6356-2020 ◽  
Author(s):  
KATARZYNA PONIEDZIAŁEK-KEMPNY ◽  
BARBARA GAJDA ◽  
IWONA RAJSKA ◽  
LECHOSŁAW GAJDA ◽  
ZDZISŁAW SMORĄG
Keyword(s):  
Case Report ◽  
In Vitro Fertilization ◽  
Control Group ◽  
First Birth ◽  
Vitro Fertilization ◽  
Research Material ◽  
Preliminary Study ◽  
Experimental Group

The aim of the study was to examine the in vivo viability of in vitro-produced (IVP) porcine embryos obtained from oocytes matured with thymosin. The research material for this study consisted of immature pig oocytes obtained from ovaries after slaughter and ejaculated semen obtained from one boar. The immature oocytes were cultured in vitro until the metaphase II stage in a medium supplemented with thymosin (TMS). The presumptive zygotes obtained were cultured in vitro for 4-40 hours. The presumptive zygotes and 2-4-cell embryos were evaluated in vivo after transferring them to synchronized recipients. After the transfer of embryos from the experimental group into 2 recipients (50 embryos into each gilt) and the transfer of 50 embryos from the control group into 1 recipient, both gilts that had received embryos obtained by in vitro fertilization of oocytes matured with TMS became pregnant and delivered a total of 16 live piglets. After the transfer of embryos from the control group, no pregnancy was achieved. In conclusion, the results of our preliminary study suggest that the maturation of pig oocytes with thymosin supports the in vivo survival of in vitro produced embryos. It is important to note, that this was the first birth of piglets obtained after transfer of IVP embryos in Poland.

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