Co-cultivation Approach to Decipher the Influence of Nitrogen-Fixing Cyanobacterium on Growth and N Uptake in Rice Crop

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Himani Priya ◽  
Dolly W. Dhar ◽  
Ranjit Singh ◽  
Sudhir Kumar ◽  
Raju Dhandapani ◽  
...  
Keyword(s):  
N Uptake ◽  
2019 ◽  
Vol 47 (2) ◽  
pp. 156-162
Author(s):  
Reginawanti Hindersah ◽  
Neni Rostini ◽  
Agustinus Marthin Kalay ◽  
Dan Arief Harsono

Exopolysaccharide (EPS) produced by nitrogen-fixing bacteria Azotobacter protect nitrogenase from oxygen. In legume,EPS plays a role in the immobilization of rhizobia to the roots. The objective of this experiment was to study the effect of EPSAzotobacter and organic matter on increasing number of nodules and biomass of soybeans grown in Inceptisols and Ultisols;as well as nitrogen-fixing bacteria population in soybean rhizosphere. The experiment was set up in a completely randomizedblock design with five replications to test combined treatments of two doses of crude EPS and organic matter. Nodule number,shoot dry weight and nitrogen uptake, as well as Azotobacter and Rhizobium population in soybean grown in Inceptisolsfollowing crude EPS and compost application, were higher than those grown in Ultisols. The application of EPS and compostIn Ultisols did not affect the number of nodule and other traits, but in Inceptisols, adding 6.25 g of compost and 20 mL of EPSto each plant increased the number of nodules and shoot weight at 42 days after planting. However, the highest N uptake wasdemonstrated by soybean received 10 mL and 20 mL EPS along with 12.5 g compost.


1999 ◽  
Vol 50 (6) ◽  
pp. 945 ◽  
Author(s):  
S. E. Ockerby ◽  
A. L. Garside ◽  
S. W. Adkins

In a previous paper, we reported that prior crops either increased or decreased the yield of paddy rice (Oryza sativa L.) and altered its response to fertiliser N. We considered that rice yield responses to prior crop might have reflected the uptake of crop residue N and the efficiency of its use to produce grain. Experiments consisted of dry-season grain or legume crops, or fallow, followed by wet-season rice (cv. Lemont); and wet-season grain or legume crops, or fallow, followed by dry-season rice. Urea at one-third of the rate required for optimum rice yield was applied at 3 stages of rice crop growth: sowing, permanent flood, and/or panicle initiation. Soil N supplied 4.1 to 6.5 g N/m2 to the rice crop, depending on the season. Rice also recovered 0 to 0.25 of the N in the residue of a prior maize crop and 0.23 to 0.57 of the N in grain legume residues or a legume green manure crop; the fraction was greater if fertiliser N was not applied. Increased N uptake was the major contributor to heavier yield. The relationship between grain yield and crop N content was mostly linear, and thus physiological efficiency of N use for rice grain production was essentially constant across the range of environments provided by fertiliser N and cropping system treatments in this study. In experiments where fertiliser N was applied, there were small effects of prior cereal and legume cropping treatments on physiological efficiency. In contrast, without fertiliser N application, physiological efficiency was increased by prior cereal and legume crops, which likely resulted from a greater congruence between the N demand of the rice crop, and the N supply from the soil and incorporated residue, when compared with a fallow treatment.


Soil Research ◽  
1989 ◽  
Vol 27 (4) ◽  
pp. 685 ◽  
Author(s):  
PE Bacon ◽  
LG Lewin ◽  
JW McGarity ◽  
EH Hoult ◽  
D Alter

The fate of 15N-labelled fertilizer applied to rice (Oryza sativa L) was studied in microplots established within two field experiments comprising a range of stubble levels, stubble management techniques, N application rates and times. The first experiment investigated uptake of soil and fertilizer N in plots where application of 0 or 100 kg N ha-1 to the previous rice crop had produced 11.5 and 16.1 t ha-1 of stubble respectively. The stubble was then treated in one of four ways-burn (no till); burn then cultivated; incorporated in autumn or incorporated at sawing. Microplots within these large plots received 60 kg ha-1 of 5% 15N enriched urea at sowing, just prior to permanent flood (PF), or just after panicle initiation (PI) of the second crop. The second experiment was undertaken within a field in which half of the plots had stubble from the previous three rice crops burned, while the other plots had all stubble incorporated. In the fourth successive rice crop, the two stubble management systems were factorially combined with three N rates (0, 70 or 140 kg N ha-1) and three application times (PF, PI or a 50 : 50 split between PF and PI). Nitrogen uptake and retention in the soil were studied within 15N-labelled microplots established within each of these large plots. Only 4% of the 15N applied at sowing in the first experiment was recovered in the rice crop, while delaying N application to PF or PI increased this to an average of 20% and 44% respectively over the two experiments. The doubling of N application rate doubled fertilizer N uptake and also increased uptake of soil N at maturity by 12 kgN ha-1. Three years of stubble incorporation increased average uptake of fertilizer and soil N in the second experiment by 5 and 12 kg N ha-1 respectively. In both experiments, the soil was the major source of N, contributing 66-96% of total N uptake. On average, in the fourth crop, 20% of fertilizer N was in the grain, 12% in the straw and 3% in the roots, while 23% was located in the top 300 mm of soil. A further 3% was in the soil below 300 mm. The remaining 39% was lost, presumably by denitrification.


3 Biotech ◽  
2014 ◽  
Vol 5 (4) ◽  
pp. 523-529 ◽  
Author(s):  
K. Bhuvaneshwari ◽  
Pawan Kumar Singh
Keyword(s):  

Agrologia ◽  
2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Mieke Rochimi Setiawati ◽  
Pujawati Suryatmana ◽  
Yuliati Machfud ◽  
Yori Tridendra

The research to determine the effect of Azolla pinnata dry weight and nitrogen-fixing endophytic bacteria on the chemical properties of soil and plants and the growth of corn plants on Inseptisols from Jatinangor. This experiment was carried out in the greenhouse of the Faculty of Agriculture, Padjadjaran University, Jatinangor, West Java. The experiment design was Randomized Block Design (RDB) in factorial pattern consisted of two factors and three replications. The first factor was dosage of Azolla pinnata compost consisted of four levels (0 g pot-1, 12.5 g pot-1, 25 g pot-1, 37.5 g pot-1). The second factor was nitrogen-fixing endophytic bacteriaconsisted three levels: without nitrogen-fixing endophytic bacteria, just used nitrogen-fixing endophytic bacteria Acinetobacter sp., used nitrogen-fixing endophytic bacteria Pseudomonas sp. The results showed that there were interactions between Azolla pinnata and N2-fixing endophytic bacteria on N concentration and N uptake of corn plants. Applicaton Azolla pinnata compost with a dosage of 37.5 g pot-1 equivalent to 6 tons ha-1 can produce the highest dry weight of corn plants. Both nitrogen-fixing endophytic bacteria and azolla compost have not been able to increase the total N of Jatinangor Insepticols.Keywords: Azolla pinnata, nitrogen-fixing endophytic bacteria, Acinetobacter sp.,   Pseudomonas sp., corn plant


2012 ◽  
pp. 1-25
Author(s):  
Michelle Castillo ◽  
Cezar Mamaril ◽  
Erlinda Paterno ◽  
Pompi Sta. Cruz ◽  
Pearl Sanchez ◽  
...  

Two field experiments were conducted in two sites with different soil properties to determine the appropriate management of rice residues coupled with cultural practices, including land preparation and fertilization to improve the nitrogen use efficiency and productivity of irrigated lowland rice. The study showed that in general, mowing of rice stubbles and either plowing or rotavating the soil did not affect the plant nutrient uptake (PNU), grain and straw yields, and yield components. In 2010 dry season, the highest PNU, yield, and return on investment (ROI) were obtained from the Minus-one Element Technique (MOET)-based fertilization compared to Nutrient Manager (NM)-based. In contrast, NM-based treatments had higher agronomic (AEN) and physiological efficiency of fertilizer nitrogen (PEN) due to higher level of N applied using the MOET compared to NM-based fertilization. In 2010 wet season, the incorporation of rice straw alone increased the uptake of P and K, similar to the crop supplied with additional inorganic fertilizer. Inorganic fertilizer treatments with or without additional chicken manure (CM) had higher grain and straw yields, yield components specifically number of tillers and panicles compared to CM and control treatments. The AEN of NSIC Rc212 was highest in plots with RIF, while CM produced the highest PEN due to higher percentage utilization or absorption of N in treatment with the least amount of N applied. Supplemental inorganic fertilizers in addition to rice residues and CM are indispensable to increase the rice yield. Incorporation of 4-5 t ha-1 rice straw for one cropping season of rice is not sufficient to significantly increase the N uptake and yield of the subsequent rice crop. The 2 month- fallow period after incorporation of straw and stubbles prior to transplanting did not affect rice yields suggesting that this length of time was sufficient to decompose the incorporated rice residues. The application of organic materials like rice straw and chicken manure was also instrumental in attaining adequate level of ROI. Therefore, continuous recycling and incorporation of rice straw in the soil during fallow period is not detrimental to the subsequent rice crop and contributes to the soil nutrient reserves which may lead to the improvement of yield and income in the long run.


2001 ◽  
Vol 41 (1) ◽  
pp. 61 ◽  
Author(s):  
Y. S. Cho ◽  
Z. R. Choe ◽  
S. E. Ockerby

Direct sowing of rice into drained soil is a relatively new farming practice, replacing transplanting, being developed for use in External Low Input Sustainable Agriculture in Korea. Field experiments were conducted to investigate the importance of tillage, sowing rate, and top-dressing of fertiliser nitrogen (N) to rice growth and grain yield in a direct-sown, rice–vetch cropping system. From 1993 to 1997, Chinese milk vetch (Astragalus sinicus L. Vetch) was grown during the winter season (October–May) at Uiryong, Korea. Until 1995 transplanted rice (Oryza sativa L.) was grown during the summer season, but in 1996 and 1997 the direct-sowing method was used. In 1996, the treatments applied to rice were either tillage or no-tillage of the paddy before the rice crop was sown, combined with 2 rates (0 or 2 g/m2) of fertiliser N top-dressed onto the paddy just after the panicle initiation stage. In 1997, the treatments were either tillage or no-tillage of the paddy before the rice crop, combined with 2 rice sowing rates (400 or 800 seeds/m2), and 3 rates (0, 2 or 4 g/m2) of fertiliser N top-dressed just after the panicle initiation stage. Rice yielded 243–435 g/m2 in 1996 and 493–678 g/m2 in 1997, the lower yield in 1996 was attributed to fewer established seedlings and productive panicles. There was no effect of tillage treatment. Across years, yield was correlated with shoot N content, and grain N-use efficiency exceeded 50 g grain/g shoot N content. Rice residues contained about 5 g N/m2 and vetch residues released 6–8 g N/m2 during the 4 weeks after submergence, therefore the release of N from residues was potentially sufficient to supply the rice crop demand. In both years, however, the yield of unfertilised rice was N limited, so increasing the shoot N content appeared to be the most important issue to be resolved in sustaining yield in External Low Input Sustainable Agriculture. A low rate of top-dressed fertiliser N (2–4 g N/m2) increased yield by increasing the number of panicles and spikelets, and to a lesser extent, 1000-grain weight. Growing rice using no-tillage practices did not limit the yield of rice, except in 1996 when no fertiliser N was applied and the N uptake by rice was low. A high sowing rate combined with top-dressed fertiliser N in 1997 reduced yield through crop lodging and an increase in the number of unproductive tillers. This problem may be overcome by the use of a variety less susceptible to lodging.


Agronomie ◽  
2002 ◽  
Vol 22 (7-8) ◽  
pp. 777-787 ◽  
Author(s):  
Graeme D. Schwenke ◽  
Warwick L. Felton ◽  
David F. Herridge ◽  
Dil F. Khan ◽  
Mark B. Peoples

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