Comparative analysis of heterochromatin distribution in wild and cultivated Abelmoschus species based on fluorescent staining methods

PROTOPLASMA ◽  
2014 ◽  
Vol 252 (2) ◽  
pp. 657-664 ◽  
Author(s):  
Keisham Merita ◽  
Joseph John Kattukunnel ◽  
Shrirang Ramchandra Yadav ◽  
Kangila Venkataramana Bhat ◽  
Satyawada Rama Rao
Keyword(s):  
Comparative Analysis ◽  
Fluorescent Staining ◽  
Staining Methods

scholarly journals A Comparative analysis of Conventional Staining methods versus Fluorescent staining in detection of pulmonary tuberculosis

2021 ◽  
Vol 39 ◽  
pp. S93-S94
Author(s):  
Jamullamudi Bhaskar Mary Ranadheer ◽  
B.V. Sivamma ◽  
P. Ratna kumari ◽  
Yona Manchikalapati
Keyword(s):  
Comparative Analysis ◽  
Pulmonary Tuberculosis ◽  
Fluorescent Staining ◽  
Staining Methods

scholarly journals CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Fajarudin Ahmad ◽  
Yuyu Suryasari Poerba
Keyword(s):  
Genetic Study ◽  
Cell Spreading ◽  
Enzymatic Digestion ◽  
Chromosome Count ◽  
Root Tip ◽  
Chromosome Counting ◽  
Fluorescent Staining ◽  
Dapi Staining ◽  
Slide Preparation ◽  
Staining Methods

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting. 

Conservation of feline semen: Part II: Cold-induced damages on spermatozoal fertilizing ability

2003 ◽  
Vol 5 (5) ◽  
pp. 257-263 ◽  
Author(s):  
G.C Luvoni ◽  
E Kalchschmidt ◽  
G Marinoni
Keyword(s):  
Special Reference ◽  
Functional Status ◽  
Limiting Factor ◽  
Fluorescent Staining ◽  
Epididymal Sperm ◽  
Freeze Thaw ◽  
Functional Integrity ◽  
Sperm Characteristics ◽  
Fertilizing Ability ◽  
Staining Methods

During the conservation of feline semen, the freeze–thaw procedure in particular is responsible for inducing severe spermatozoal damage, which diminishes fertilizing ability. Therefore, cold-induced damage represents a limiting factor for the conservation of semen, particularly semen from felids, which are often affected by teratospermia. In this article, feline sperm characteristics are reported, with special reference to motility and morphology, which are more likely to be affected by conservation protocols; and moreover, the causes of cold-induced damages are described. Attention has been focused on methods to evaluate functional integrity of spermatozoa, and those applied to cat semen are reviewed. Among these, a rather recently developed technique involves fluorescent staining methods, and in particular chlortetracycline. The chlortetracycline assay applied to cryopreserved cat epididymal sperm shows that it is suitable to evaluate the functional status of cat sperm.

scholarly journals THE USE OF BASIC POLYSACCHARIDES IN HISTOCHEMISTRY AND CYTOCHEMISTRY; II. THE DEMONSTRATION OF ACIDIC POLYSACCHARIDES IN TISSUE SECTIONS USING FLUORESCEIN-LABELLED DEACETYLATED CHITIN

1962 ◽  
Vol 10 (1) ◽  
pp. 14-18 ◽  
Author(s):  
SIDNEY P. KENT ◽  
E. EDWARD EVANS
Keyword(s):  
Connective Tissue ◽  
Acridine Orange ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Fluorescent Staining ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Acidic Polysaccharides ◽  
Staining Methods ◽  
Formalin Fixed

A method for demonstrating acidic polysaccharides in formalin fixed parafin embedded tissue sections using fluorescein labelled deacetylated chitin is described. Epithelial and connective tissue acidic polysaccharides have been studied in a number of organs. The distribution of acidic polysaccharides as seen with fluorescein-labelled deacetylated chitin is similar to results obtained with alcian blue-periodic acid Schiff and fluorescein-labelled Aspergillus polysaccharide. Previously reported fluorescent staining methods for acidic polysaccharides such as iron-hematoxylin-acridine orange and atabrine as well as fluorescein-labelled deacetylated chitin may prove to be useful adjuncts to the light microscopic methods of demonstrating mucins. Other possible uses of deacetylated chitin, a colorless macro-cation, in histochemistry and cytochemistry are noted.

scholarly journals COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE

2018 ◽  
Vol 11 (4) ◽  
pp. 110
Author(s):  
Neelu Sree P ◽  
Terin J ◽  
Himadri Dutta ◽  
Kalyani M
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sputum Sample ◽  
Diagnostic Value ◽  
Fluorescent Staining ◽  
Concentration Technique ◽  
Before And After ◽  
Auramine O ◽  
Staining Methods ◽  
Staining Techniques ◽  
Microscopy Examination

 Objectives: The objectives of the study were to find out the presence of Mycobacterium tuberculosis in sputum samples using Ziehl-Neelsen (ZN), Kinyoun’s, and (auramine) fluorescent staining and to compare the three staining techniques with and without Petroff’s concentration and to find out the most preferable staining of M. tuberculosis. Methods: Sputum sample was collected and concentrated by 4% NaOH (Petroff’s concentration). Microscopic examination of the sample was done before concentration and after concentration by ZN staining, Kinyoun’s staining, and fluorescent staining (Auramine). Grading of acid-fast bacilli (AFB) by three staining was done before and after concentration according to RNTCP guidelines.Results: Total of 452 sputum samples were collected and subjected to microscopy examination by ZN, Kinyoun’s, and fluorescent staining methods to compare the presence or absence of AFB with or without concentration. Among the 452 sputum samples, total of 67 (15.6%) sputum samples were positive for the presence of AFB. Majority 40.3% (n=21) of TB positive patients were observed in the age group 51–60 years. The results of Auramine-O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100% and specificity 95.6%.Conclusion: The use of fluorescent staining significantly increases the diagnostic value of the smear, particularly where there are low-density bacilli which may escape detection on ZN stained smears.

scholarly journals ON THE HISTOCHEMISTRY OF AZO GROUP-FREE THIAZOLE DYES

1967 ◽  
Vol 15 (3) ◽  
pp. 172-180 ◽  
Author(s):  
GABRIEL KELIÉNYI
Keyword(s):  
Chemical Composition ◽  
Dye Molecules ◽  
Fluorescent Staining ◽  
Number Of Components ◽  
Related Substances ◽  
Azo Group ◽  
Thioflavine S ◽  
Staining Methods ◽  
Physicochemical Methods ◽  
Thioflavine T

Analysis of primuline, thioflavine S and thioflavine T acid and basic azo group-free thiazole dyes showed that they were built up from a number of components which were characterized by physicochemical methods. It was found that the isolated components, as well as related substances of known chemical composition, have characteristic staining properties. Factors involved in the staining mechanism of the dyes and of components—dye concentration, pH, aggregation of the dye molecules—were investigated and their roles are discussed. Selectivity of these fluorescent staining methods was also studied.

scholarly journals Bacterial Community Structure and Their Activity in Tropical Rivers Determined by Fluorescent Staining Methods.

1999 ◽  
Vol 22 (12) ◽  
pp. 1001-1004
Author(s):  
Takehiko KENZAKA ◽  
Nobuyasu YAMAGUCHI ◽  
Kazuma FUJIMOTO ◽  
Hiroshi TADOKORO ◽  
Masao NASU
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Tropical Rivers ◽  
Fluorescent Staining ◽  
Staining Methods

scholarly journals Reversibility of membrane permeabilization upon pulsed electric field treatment in Lactobacillus plantarum WCFS1

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
E. M. J. Vaessen ◽  
R. A. H. Timmermans ◽  
M. H. Tempelaars ◽  
M. A. I. Schutyser ◽  
H. M. W. den Besten
Keyword(s):  
Electric Field ◽  
Lactobacillus Plantarum ◽  
Staining Method ◽  
Membrane Integrity ◽  
Pulsed Electric Field ◽  
Cellular Membrane ◽  
Membrane Permeabilization ◽  
Fluorescent Staining ◽  
Treatment Conditions ◽  
Staining Methods

AbstractPulsed electric field (PEF) treatment, or electroporation, can be used to load molecules into cells. The permeabilizing effect of the PEF treatment on the cellular membrane can be either reversible or irreversible depending on the severity of the PEF treatment conditions. The influence of PEF on the reversibility of membrane permeabilization in Lactobacillus plantarum WCFS1 by two different fluorescent staining methods was investigated in this study. Whereas staining with propidium iodide (PI) before and after PEF treatment indicated small reversible permeabilized fractions of maximum 14%, the use of a double staining method with PI and SYTOX Green suggested larger reversible permeabilized fractions up to 40% of the population. This difference shows that the choice for a fluorescent staining method affects the conclusions drawn regarding reversibility of membrane permeabilization. Additionally, the effect of PEF treatment conditions on membrane integrity was compared, indicating a relation between critical electric field strength, cell size and membrane permeabilization. Overall this study showed the possibilities and limitations of fluorescent membrane integrity staining methods for PEF studies.

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