Mechanical bending-induced tension wood formation with reduced lignin biosynthesis in Liriodendron tulipifera

2009 ◽  
Vol 55 (6) ◽  
pp. 401-408 ◽  
Author(s):  
Hyunjung Jin ◽  
Mi Kwon
Keyword(s):  
Tension Wood ◽  
Lignin Biosynthesis ◽  
Liriodendron Tulipifera ◽  
Wood Formation ◽  
Mechanical Bending

scholarly journals RNA-Seq analysis of differential gene expression in Betula luminifera xylem during the early stages of tension wood formation

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5427 ◽  
Author(s):  
Miaomiao Cai ◽  
Huahong Huang ◽  
Fei Ni ◽  
Zaikang Tong ◽  
Erpei Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tension Wood ◽  
Molecular Mechanisms ◽  
Southern China ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Cellulose Synthesis ◽  
Early Stages ◽  
Transcriptional Changes ◽  
Betula Luminifera

Background Betula luminifera H. Winkler, which is widely distributed in southern China, is an economically important broadleaf tree species. However, little genomic information of B. luminifera is available, and little is known about the molecular mechanisms of wood formation in this species. Meanwhile, few efforts have focused on investigating the early transcriptional changes during tension wood formation in woody plants. Results A reference transcriptome dataset was first generated containing 45,700 Unigenes, and 35,135 (76.9%) Unigenes were annotated by a BLAST similarity search against four public databases. Then, based on an anatomical investigation, the global gene expression changes during the early stages of tension wood formation were analyzed. Gene expression profiling showed that a total of 13,273 Unigenes were differentially regulated during the early stages of tension wood formation. Most genes involved in cellulose and lignin biosynthesis were highlighted to reveal their biological importance in tension wood formation. In addition, the transcription levels of many genes involved in the auxin response pathway were significantly changed during the early stages of tension wood formation. Furthermore, 18 TFs co-expressed with key enzymes of cellulose synthesis were identified. Conclusions Our results revealed the transcriptional changes associated with TW formation and identified potential key genes in the regulation of this process. These results will help to dissect the molecular mechanism of wood formation and provide key candidate genes for marker-assisted selection in B. luminifera.

scholarly journals TheEucalyptuslinker histone variant EgH1.3 cooperates with the transcription factor EgMYB1 to control lignin biosynthesis during wood formation

2016 ◽  
Vol 213 (1) ◽  
pp. 287-299 ◽  
Author(s):  
Marçal Soler ◽  
Anna Plasencia ◽  
Romain Larbat ◽  
Cécile Pouzet ◽  
Alain Jauneau ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Histone Variant

scholarly journals Cloning and Functional Analysis of Lignin Biosynthesis Genes Cf4CL and CfCCoAOMT in Cryptomeria fortunei

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 619 ◽  
Author(s):  
Zhenhao Guo ◽  
Hui Hua ◽  
Jin Xu ◽  
Jiaxing Mo ◽  
Hui Zhao ◽  
...  
Keyword(s):  
Gene Editing ◽  
Bioinformatics Analysis ◽  
Southern China ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Primary Component ◽  
Vascular Cambium ◽  
Timber Species ◽  
Wood Production ◽  
Lignin Synthesis

Cryptomeria fortunei, also known as the Chinese cedar, is an important timber species in southern China. The primary component of its woody tissues is lignin, mainly present in secondary cell walls. Therefore, continuous lignin synthesis is crucial for wood formation. In this study, we aimed to discover key genes involved in lignin synthesis expressed in the vascular cambium of C. fortunei. Through transcriptome sequencing, we detected expression of two genes, 4CL and CCoAOMT, known to be homologous to enzymes involved in the lignin synthesis pathway. We studied the function of these genes through bioinformatics analysis, cloning, vascular cambium expression analysis, and transgenic cross-species functional validation studies. Our results show that Cf4CL and CfCCoAOMT do indeed function in the pathway of lignin synthesis and likely perform this function in C. fortunei. They are prime candidates for future (gene-editing) studies aimed at optimizing C. fortunei wood production.

scholarly journals Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots

Biomolecules ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 156 ◽  
Author(s):  
Wan-Long Su ◽  
Na Liu ◽  
Li Mei ◽  
Jie Luo ◽  
Yi-Jie Zhu ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Theoretical Basis ◽  
Reference Data ◽  
Profile Analysis ◽  
Expression Patterns ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Boron Deficiency ◽  
Rna Seq ◽  
Lignin Accumulation

To uncover the transcriptomic mechanism of lignin accumulation caused by boron deficiency (BD), Nanlin895 (Populus × euramericana “Nanlin895”) was subjected to control (CK, 0.25 mg·L−1) and BD (0 mg·L−1) treatments for 3 days. RNA-Seq was carried out to survey the expression patterns of the lignin-regulated biosynthetic genes in response to BD. The results showed that 5946 genes were identified as differentially expressed genes (DEGs), 2968 (44.2%) of which were upregulated and 3318 (55.8%) of which were downregulated in response to BD. Among them, the expression of lignin monomer biosynthetic (PAL, CCR, CAD, COMT, F5H, PER/LAC) and modulated genes, for example, transcription factors (MYBs) and hormone signal regulating genes (GIDs, histidine kinase 1, coronatine-insensitive protein 1), were upregulated, and some hormone signal regulating genes, such as AUXs and BR-related (sterol methyltransferases), were downregulated under BD treatment. There are also some genes that were screened as candidates for an association with wood formation, which will be used for the further analysis of the function of lignin formation. These results provide an important theoretical basis and reference data in plant for further research on the mechanism of lignin accumulation under BD.

Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar

2015 ◽  
Vol 14 (8) ◽  
pp. 3188-3203 ◽  
Author(s):  
Mélanie Mauriat ◽  
Jean-Charles Leplé ◽  
Stéphane Claverol ◽  
Jérôme Bartholomé ◽  
Luc Negroni ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Tension Wood ◽  
Wood Formation

ROLES OF AUXIN AND GIBBERELLIN IN GRAVITY-INDUCED TENSION WOOD FORMATION IN AESCULUS TURBINATA SEEDLINGS

IAWA Journal ◽  
2004 ◽  
Vol 25 (3) ◽  
pp. 337-347 ◽  
Author(s):  
Sheng Du ◽  
Hiroki Uno ◽  
Fukuju Yamamoto
Keyword(s):  
Tension Wood ◽  
Auxin Transport ◽  
Histological Analysis ◽  
Synergistic Effects ◽  
Wood Formation ◽  
Wood Fibers ◽  
Lower Side ◽  
Inhibitory Effects ◽  
Auxin Action ◽  
Ga Biosynthesis

The lowest nodes of 6-week-old Aesculus turbinata seedlings were treated with uniconazole-P, an inhibitor of gibberellin (GA) biosynthesis, or a mixture of uniconazole-P and GA3 in acetone solution. To the seedling stems, an inhibitor of auxin transport (NPA) or inhibitors of auxin action (raphanusanin or MBOA) were applied in lanolin paste. The seedlings were tilted at a 45° angle and kept for 10 weeks before histological analysis. Decreases in both normal and tension wood formation followed the application of uniconazole-P. The application of GA3 together with uniconazole-P partially negated the effect of uniconazole-P alone. The application of NPA inhibited tension wood formation at, above, and below the lanolin-treated portions. The treatment of raphanusanin or MBOA also resulted in decreases in tension wood formation at the treated portions. The inhibitory effects of these chemicals applied on the upper side of tilted stems or around the entire stem were greater than on the lower side. The application of uniconazole-P in combination with raphanusanin, MBOA or NPA showed synergistic effects on the inhibition of tension wood formation. The results suggest that both auxin and GA regulate the quantitative production of tension wood fibers and are essential to tension wood formation.

Ethylene Evolution Changes in TiltedFraxinus mandshuricaRupr. var.japonicaMaxim. Seedlings in Relation to Tension Wood Formation

2009 ◽  
Vol 51 (7) ◽  
pp. 707-713 ◽  
Author(s):  
Sha Jiang ◽  
Ke Xu ◽  
Na Zhao ◽  
Shu-Xin Zheng ◽  
Yan-Ping Ren ◽  
...  
Keyword(s):  
Tension Wood ◽  
Wood Formation ◽  
Ethylene Evolution

scholarly journals Expression of three phenylpropanoid pathway genes in Scots pine (Pinus sylvestris L.) in open-pollinated families with differing relative wood densities during early and late wood formation

2015 ◽  
Vol 64 (1-6) ◽  
pp. 148-159 ◽  
Author(s):  
K. Kanberga-Silina ◽  
A. Jansons ◽  
Dainis Rungis
Keyword(s):  
Gene Expression ◽  
Wood Density ◽  
Scots Pine ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Wood Formation ◽  
Phenylpropanoid Pathway ◽  
Structural Development ◽  
Late Wood ◽  
Forestry Production

Abstract Wood volume and quality are the most important aspects of commercial forestry production, and studies of wood formation are important in order to increase the value and efficiency of forestry production. The phenylpropanoid pathway produces various compounds with diverse functions both for plant defence against biotic and abiotic stress as well as structural development. One of the main roles is monolignol production for lignin biosynthesis, which is a crucial aspect of wood formation. For this study three candidate genes involved in lignin biosynthesis were selected: phenylalanine ammonialyase (PAL1), cinnamyl alcohol dehydrogenase (CAD) and cinnamoyl-CoA reductase (CCR). Candidate gene expression was analysed in selected individuals with high and low wood density from open-pollinated Scots pine families during early wood (EW) and late wood (LW) formation and correlation between expression of these genes, total lignin content, and wood density was determined. Wood density values for analysed trees were similar within tree families but differed significantly between families with high and low wood density (p=1,06E-20). Wood density was slightly negatively correlated with lignin content (r=-0.36, p=0.038), but only in individuals in the high density wood group. In trees with low wood density, expression of the CAD gene was significantly lower in late wood formation compared to early wood (p=0.00179). In trees with high wood density, expression of the PAL1 gene was five times higher during early wood formation compared to late wood formation. A positive correlation was detected between PAL1 and CCR gene expression during early wood formation (r=0.804) and late wood formation (r=0.466).

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