scholarly journals Diversity of carbapenem-resistant Klebsiella pneumoniae ST14 and emergence of a subgroup with KL64 capsular locus in the Arabian Peninsula

2021 ◽  
Author(s):  
Shaimaa F. Mouftah ◽  
Tibor Pál ◽  
Paul G. Higgins ◽  
Akela Ghazawi ◽  
Youssef Idaghdour ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Klebsiella Pneumoniae ◽  
United Arab Emirates ◽  
Core Genome ◽  
Arabian Peninsula ◽  
Illumina Miseq ◽  
Carbapenem Resistant ◽  
Distinct Cluster ◽  
Recent Emergence ◽  
Miseq Platform

AbstractTo understand the reasons of successful spread of carbapenem-resistant Klebsiella pneumoniae ST14 (CRKP-ST14) in countries of the Arabian Peninsula, the resistome, capsular locus, carbapenemase carrying plasmid types, and core genome of isolates from the region were compared to global isolates. Thirty-nine CRKP-ST14 strains isolated from 13 hospitals in the United Arab Emirates, Bahrain, and Saudi Arabia were selected for whole genome sequencing on Illumina MiSeq platform based on the variety of carbapenemase genes carried and plasmids bearing these genes. Their resistome, capsular locus, and core genome MLST were compared to 173 CRKP-ST14 genomes available in public databases. The selected 39 CRKP-ST14 produced either NDM-1, OXA-48, OXA-162, OXA-232, KPC-2, or co-produced NDM-1 and an OXA-48-like carbapenemase. cgMLST revealed three clusters: 16 isolates from five UAE cities (C1), 11 isolates from three UAE cities and Bahrain (C2), and 5 isolates from Saudi Arabia (C3), respectively, and seven singletons. Resistance gene profile, carbapenemase genes, and their plasmid types were variable in both C1 and C2 clusters. The majority of CRKP-ST14 had KL2, but members of the C2 cluster and two further singletons possessed KL64 capsular locus. Based on cgMLST comparison of regional and global isolates, CRKP-ST14 with KL64 from four continents formed a distinct cluster, suggesting a recent emergence and spread of this variant. Our findings confirmed clonal transmission coupled with likely horizontal gene transfer in carbapenem-resistant Klebsiella pneumoniae ST14. Dissemination of this genetically flexible, highly resistant clone warrants further monitoring.

scholarly journals Antimicrobial Resistance and Genomic Characterization of OXA-48- and CTX-M-15-Co-Producing Hypervirulent Klebsiella pneumoniae ST23 Recovered from Nosocomial Outbreak

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 862
Author(s):  
Elvira Shaidullina ◽  
Andrey Shelenkov ◽  
Yuri Yanushevich ◽  
Yulia Mikhaylova ◽  
Dmitriy Shagin ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Illumina Miseq ◽  
Type I ◽  
Nosocomial Outbreak ◽  
Recent Emergence ◽  
Genomic Characterization ◽  
Global Threat ◽  
High Level ◽  
Sepsis And Septic Shock

Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate, MAR14-456, representative of a nosocomial outbreak in Moscow, Russia, that was recovered from a postoperative wound in a patient who later developed multiple abscesses, fatal sepsis, and septic shock. Broth microdilution testing revealed decreased susceptibility of MAR14-456 to carbapenems (MICs 0.5–2 mg/L) and a high-level resistance to most β-lactams, β-lactam-β-lactamase-inhibitor combinations, and non-β-lactam antibiotics, except ceftazidime-avibactam, amikacin, tigecycline, and colistin. Whole-genome sequencing using Illumina MiSeq and ONT MinION systems allowed to identify and completely assemble two conjugative resistance plasmids, a typical ‘European’ epidemic IncL/M plasmid that carries the gene of OXA-48 carbapenemase, and an IncFIIK plasmid that carries the gene of CTX-M-15 ESBL and other resistance genes. MLST profile, capsular, lipopolysaccharide, virulence genes encoded on chromosome and IncHI1B/FIB plasmid, and the presence of apparently functional type I-E* CRISPR-Cas system were all characteristic of hvKp ST23, serotype K1-O1v2. Phylogenetic analysis showed the closest relatedness of MAR14-456 to ST23 isolates from China. This report highlights the threat of multiple resistance acquisition by hvKp strain and its spread as a nosocomial pathogen.

scholarly journals Comparative Pathogenomics of Aeromonas veronii from Pigs in South Africa: Dominance of the Novel ST657 Clone

2020 ◽  
Vol 8 (12) ◽  
pp. 2008
Author(s):  
Yogandree Ramsamy ◽  
Koleka P. Mlisana ◽  
Daniel G. Amoako ◽  
Akebe Luther King Abia ◽  
Mushal Allam ◽  
...  
Keyword(s):  
South Africa ◽  
Orthologous Gene ◽  
Gene Clusters ◽  
Illumina Miseq ◽  
Aeromonas Veronii ◽  
Carbapenem Resistant ◽  
Kwazulu Natal ◽  
Miseq Platform ◽  
Differential Ability ◽  
Potential Pathogenicity

The pathogenomics of carbapenem-resistant Aeromonas veronii (A. veronii) isolates recovered from pigs in KwaZulu-Natal, South Africa, was explored by whole genome sequencing on the Illumina MiSeq platform. Genomic functional annotation revealed a vast array of similar central networks (metabolic, cellular, and biochemical). The pan-genome analysis showed that the isolates formed a total of 4349 orthologous gene clusters, 4296 of which were shared; no unique clusters were observed. All the isolates had similar resistance phenotypes, which corroborated their chromosomally mediated resistome (blaCPHA3 and blaOXA-12) and belonged to a novel sequence type, ST657 (a satellite clone). Isolates in the same sub-clades clustered according to their clonal lineages and host. Mobilome analysis revealed the presence of chromosome-borne insertion sequence families. The estimated pathogenicity score (Pscore ≈ 0.60) indicated their potential pathogenicity in humans. Furthermore, these isolates carried several virulence factors (adherence factors, toxins, and immune evasion), in different permutations and combinations, indicating a differential ability to establish infection. Phylogenomic and metadata analyses revealed a predilection for water environments and aquatic animals, with more recent reports in humans and food animals across geographies, making A. veronii a potential One Health indicator bacterium.

scholarly journals Molecular Characterization of Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae in the Countries of the Gulf Cooperation Council: Dominance of OXA-48 and NDM Producers

2014 ◽  
Vol 58 (6) ◽  
pp. 3085-3090 ◽  
Author(s):  
Hosam M. Zowawi ◽  
Anna L. Sartor ◽  
Hanan H. Balkhy ◽  
Timothy R. Walsh ◽  
Sameera M. Al Johani ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
United Arab Emirates ◽  
Gulf Cooperation Council ◽  
Mechanisms Of Resistance ◽  
Antibiotic Resistant ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Resistant Genes ◽  
Gcc Countries

ABSTRACTThe molecular epidemiology and mechanisms of resistance of carbapenem-resistantEnterobacteriaceae(CRE) were determined in hospitals in the countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, United Arab Emirates, Oman, Qatar, Bahrain, and Kuwait. Isolates were subjected to PCR-based detection of antibiotic-resistant genes and repetitive sequence-based PCR (rep-PCR) assessments of clonality. Sixty-two isolates which screened positive for potential carbapenemase production were assessed, and 45 were found to produce carbapenemase. The most common carbapenemases were of the OXA-48 (35 isolates) and NDM (16 isolates) types; 6 isolates were found to coproduce the OXA-48 and NDM types. No KPC-type, VIM-type, or IMP-type producers were detected. Multiple clones were detected with seven clusters of clonally relatedKlebsiella pneumoniae. Awareness of CRE in GCC countries has important implications for controlling the spread of CRE in the Middle East and in hospitals accommodating patients transferred from the region.

An updated catalogue of the Histeridae (Insecta: Coleoptera) of the Arabian Peninsula, with biogeographical remarks

Zootaxa ◽  
2006 ◽  
Vol 1157 (1) ◽  
pp. 1 ◽  
Author(s):  
FABIO PENATI ◽  
PIERPAOLO VIENNA
Keyword(s):  
Saudi Arabia ◽  
United Arab Emirates ◽  
Arabian Peninsula ◽  
Transitional Zone ◽  
Distributional Pattern ◽  
Mediterranean Species ◽  
Public And Private ◽  
Palearctic Species ◽  
First Time ◽  
Private Collections

The histerid fauna of the Arabian Peninsula is evaluated. As a result of the study of specimens recently collected in Oman by the scientific expeditions of the Museum of Natural History and Territory of Calci (University of Pisa, Italy), and compiling new data from 15 public and private collections, and literature records, the total number of Histeridae known from the Arabian Peninsula now stands at 67 species, without taking into account dubious species and undetermined taxa (9).Of these 67 species, in total 18 are reported for the first time: 4 for the Arabian Peninsula [Teretrius (Neotepetrius) parasita Marseul, Saprinus (Saprinus) flexuosofasciatus Motschulsky, Chalcionellus hauseri (Schmidt), Hypocacculus (Nessus) hosseinius (Théry)], 9 for Oman [Pachylister luctuosus (Marseul), Hister castus Lewis, Atholus bimaculatus Linnaeus, Atholus scutellaris (Erichson), Saprinus (Saprinus) splendens (Paykull), Hypocacculus (Colpellus) praecox (Erichson), Hypocacculus (Hypocacculus) harmonicus (Marseul), Hypocacculus (Hypocacculus) metallescens (Erichson), Hypocaccus (Hypocaccus) fochi (Auzat)], 2 for Yemen [Saprinus (Saprinus) tenuistrius tenuistrius Marseul, Chalcionellus tunisius (Marseul)] and 3 for Kuwait [Saprinus (Saprinus) moyses Marseul, Pholioxenus mesopotamicus Olexa, Paravolvulus syphax (Reitter)].Histeridae are now known for four national states (Saudi Arabia, Yemen, Oman and Kuwait), while there are no records for Qatar or United Arab Emirates. An updated comprehensive catalogue of the histerid fauna of the Arabian Peninsula is provided.Analysis of faunal affinities shows that it mostly consists of Palearctic species (24 species, equal to 35.9%), followed by Afrotropical and Afrotropico-Mediterranean species (22 species, 32.8%), and cosmopolitan/subcosmopolitan species (7 species, 10.4%); endemics stand at 5 species (7.5%), while species of undefined chorology at 9 (13.4%). On the whole these numbers fit well the largely accepted biogeographical assumption that indicates the Arabian Peninsula as a transitional zone between the Regions Palearctic, Afrotropic and, to a lesser degree, Indo-Malay (e.g., Büttiker 1979). Moreover, a distinctive distributional pattern has been recognised: “Afrotropical” Histeridae are almost exclusively present in the southern and south-western parts of the peninsula, while “Palearctic” Histeridae are mostly present in the inner central and south-eastern parts of the peninsula.Finally, the synonymy Saprinus (Saprinus) osiris Marseul, 1862 = Saprinus (Saprinus) ornatus Erichson, 1834 stated by Müller (1933), but neglected by Mazur (1976, 1984, 1994, 1997), is considered valid.

scholarly journals Comparative analysis of Clinial Carbapenem- Resistant (CR), Hypermucoviscous (HM) and CR-HM Klebsiella pneumoniae isolates in China

2020 ◽  
Author(s):  
Jun-Ying Zhu ◽  
Guang-Yu Wang ◽  
Qing Wei ◽  
Zhen Shen ◽  
Qiong Li ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Virulence Genes ◽  
Carbapenem Resistance ◽  
Resistance Rate ◽  
Molecular Characteristics ◽  
Carbapenem Resistant ◽  
Recent Emergence ◽  
String Test

Abstract Background: Although carbapenem-resistant Klebsiella pneumoniae (CRKP) and hypermucoviscous K. pneumoniae (HMKP) were largely non-overlapping, the recent emergence of CR-HMKP has raised great alarm in the world. We compared the molecular characteristics of CRKP, HMKP and CR-HMKP isolates.Results: 220 cases of K. pneumoniae isolates was collected and identified between Jan 2015 and Dec 2016 from Renji Hospital. Carbapenem resistance test and string test were performed to screen CRKP, HMKP and CR-HMKP isolates. All the CRKP, HMKP and CR-HMKP isolates were investigated for capsular genotyping, virulence genes and resistance genes by PCR and DNA sequencing. Multilocus sequence typing (MLST) was used to characterize isolates sequence types (STs). Serum killing assay and mouse lethality assay were respectively performed to confirm the virulence of the isolates in vitro and in vivo. Of 220 K. pneumoniae，71 HMKP, 84 CRKP and 8 CR-HMKP were identified. Resistance rate to carbapenems was significantly higher in CRKP than HMKP and CR-HMKP. For MLST and serotyping, ST23 (26.8%)，K1 (33.8%) and K2 (23.9%) serotypes were the most common in HMKP isolates while ST11 (84.5%, 100%) and K-nontypable (91.6%, 100%) were the predominant types in CRKP and CR-HMKP isolates. The existence of virulence genes rmpA, magA and iutA was significantly higher in HMKP while the prevalence of resistance gene blaKPC-2 was higher in CRKP and CR-HMKP. Virulence test in vivo and in vitro both showed the lower virulence of CRKP and CR-HMKP compared to HMKP.Conclusions: In spite of low virulence, the emergence of CR-HMKP indicates a confluence of hypermucoviscous phenotype and carbapenem resistance. Furthermore, the similar molecular characteristics between CRKP and CR-HMKP suggested that CR-HMKP might evolve from CRKP.

The New Old Imperialism in the Arabian Peninsula

2020 ◽  
Vol 10 (1) ◽  
pp. 101-116
Author(s):  
Steven C. Caton
Keyword(s):  
Saudi Arabia ◽  
Cold War ◽  
United Arab Emirates ◽  
Coming Out ◽  
Arabian Peninsula ◽  
Marxist Theory ◽  
Proxy War ◽  
The Face ◽  
Hardt And Negri ◽  
The Cold War

Abstract This essay argues that the horrific war in Yemen (2015–present) waged by Saudi Arabia and the United Arab Emirates against the Houthis who control the northern part of the country is not really a Sunni-Schi’a conflict or a proxy war against Iran or a replay of the Cold War—all of which have been put forward to explain it—but rather is better understood as the actions of belligerent imperialist powers located in the Arabian Peninsula, acting in their own right (rather than as puppets of Western powers). Such an explanation, however, flies in the face of what we have understood imperialism to be historically. This essay looks at two understandings of imperialism, one coming out of Marxist theory and another out of a Foucauldian understanding of power as developed by Hardt and Negri in their book Empire (2000), and then goes on to show that both are helpful, though in different ways, in explaining the Yemen war as an imperialist intervention.

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