scholarly journals Inhibitory effects of ultrasound irradiation on Staphylococcus epidermidis biofilm

2021 ◽  
Author(s):  
Harumi Koibuchi ◽  
Yasutomo Fujii ◽  
Yusuke Sato’o ◽  
Takashi Mochizuki ◽  
Toshiyuki Yamada ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bloodstream Infections ◽  
Ultrasound Irradiation ◽  
Bottom Surface ◽  
Bacterial Biofilms ◽  
Inhibitory Effects ◽  
Lower Intensity ◽  
Remarkable Effect ◽  
Continuous Waves

Abstract Purpose We aimed to investigate whether low-intensity continuous and pulsed wave ultrasound (US) irradiation can inhibit the formation of Staphylococcus epidermidis biofilms, for potential application in the treatment of catheter-related bloodstream infections (CRBSI). Methods S. epidermidis biofilms that formed on the bottom surfaces of 6-well plates were irradiated on the bottom surface using the sound cell incubator system for different intervals of time. Results US irradiation with continuous waves for 24 h notably inhibited biofilm formation (p < 0.01), but the same US irradiation for 12 h had no remarkable effect. Further, double US irradiation with pulsed waves for 20 min inhibited biofilm formation by 33.6%, nearly two-fold more than single US irradiation, which reduced it by 17.9%. Conclusion US irradiation of a lower intensity (ISATA = 6–29 mW/cm2) than used in a previous study and lower than recommended by the Food and Drug Administration shows potential for preventing CRBSI caused by bacterial biofilms.

scholarly journals Depolymerization of β-1,6-N-Acetyl-d-Glucosamine Disrupts the Integrity of Diverse Bacterial Biofilms

2005 ◽  
Vol 187 (1) ◽  
pp. 382-387 ◽  
Author(s):  
Yoshikane Itoh ◽  
Xin Wang ◽  
B. Joseph Hinnebusch ◽  
James F. Preston ◽  
Tony Romeo
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Enzymatic Hydrolysis ◽  
Pseudomonas Fluorescens ◽  
Yersinia Pestis ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Biofilms ◽  
First Time ◽  
Hydrolysis Of

ABSTRACT Polymeric β-1,6-N-acetyl-d-glucosamine (poly-β-1,6-GlcNAc) has been implicated as an Escherichia coli and Staphylococcus epidermidis biofilm adhesin, the formation of which requires the pgaABCD and icaABCD loci, respectively. Enzymatic hydrolysis of poly-β-1,6-GlcNAc, demonstrated for the first time by chromatography and mass spectrometry, disrupts biofilm formation by these species and by Yersinia pestis and Pseudomonas fluorescens, which possess pgaABCD homologues.

scholarly journals Small Molecules Produced by Commensal Staphylococcus epidermidis Disrupt Formation of Biofilms by Staphylococcus aureus

2019 ◽  
Vol 86 (5) ◽  
Author(s):  
Thaís Glatthardt ◽  
Juliana Curityba de Mello Campos ◽  
Raiane Cardoso Chamon ◽  
Thiago Freitas de Sá Coimbra ◽  
Giulia de Almeida Rocha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bloodstream Infections ◽  
Proteinase K ◽  
Antibiofilm Activity ◽  
Skin Microbiome ◽  
Chronic Infections ◽  
Content Type ◽  
Treatment And Prevention

ABSTRACT The microbiota influences host health through several mechanisms, including protecting it from pathogen colonization. Staphylococcus epidermidis is one of the most frequently found species in the skin microbiota, and its presence can limit the development of pathogens such as Staphylococcus aureus. S. aureus causes diverse types of infections ranging from skin abscesses to bloodstream infections. Given the increasing prevalence of S. aureus drug-resistant strains, it is imperative to search for new strategies for treatment and prevention. Thus, we investigated the activity of molecules produced by a commensal S. epidermidis isolate against S. aureus biofilms. We showed that molecules present in S. epidermidis cell-free conditioned media (CFCM) caused a significant reduction in biofilm formation in most S. aureus clinical isolates, including all 4 agr types and agr-defective strains, without any impact on growth. S. epidermidis molecules also disrupted established S. aureus biofilms and reduced the antibiotic concentration required to eliminate them. Preliminary characterization of the active compound showed that its activity is resistant to heat, protease inhibitors, trypsin, proteinase K, and sodium periodate treatments, suggesting that it is not proteinaceous. RNA sequencing revealed that S. epidermidis-secreted molecules modulate the expression of hundreds of S. aureus genes, some of which are associated with biofilm production. Biofilm formation is one of the main virulence factors of S. aureus and has been associated with chronic infections and antimicrobial resistance. Therefore, molecules that can counteract this virulence factor may be promising alternatives as novel therapeutic agents to control S. aureus infections. IMPORTANCE S. aureus is a leading agent of infections worldwide, and its main virulence characteristic is the ability to produce biofilms on surfaces such as medical devices. Biofilms are known to confer increased resistance to antimicrobials and to the host immune responses, requiring aggressive antibiotic treatment and removal of the infected surface. Here, we investigated a new source of antibiofilm compounds, the skin microbiome. Specifically, we found that a commensal strain of S. epidermidis produces molecules with antibiofilm activity, leading to a significant decrease of S. aureus biofilm formation and to a reduction of previously established biofilms. The molecules potentiated the activity of antibiotics and affected the expression of hundreds of S. aureus genes, including those associated with biofilm formation. Our research highlights the search for compounds that can aid us in the fight against S. aureus infections.

scholarly journals Lucilia sericata Chymotrypsin Disrupts Protein Adhesin-Mediated Staphylococcal Biofilm Formation

2012 ◽  
Vol 79 (4) ◽  
pp. 1393-1395 ◽  
Author(s):  
Llinos G. Harris ◽  
Yamni Nigam ◽  
James Sawyer ◽  
Dietrich Mack ◽  
David I. Pritchard
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Lucilia Sericata ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Formation Mechanisms ◽  
Chronic Infections ◽  
Content Type ◽  
Effective Activity

ABSTRACTStaphylococcus aureusandStaphylococcus epidermidisbiofilms cause chronic infections due to their ability to form biofilms. The excretions/secretions ofLucilia sericatalarvae (maggots) have effective activity for debridement and disruption of bacterial biofilms. In this paper, we demonstrate how chymotrypsin derived from maggot excretions/secretions disrupts protein-dependent bacterial biofilm formation mechanisms.

scholarly journals Involvement of the Iron-Regulated Loci hts and fhuC in Biofilm Formation and Survival of Staphylococcus epidermidis within the Host

2022 ◽  
Author(s):  
Fernando Oliveira ◽  
Tânia Lima ◽  
Alexandra Correia ◽  
Ana Margarida Silva ◽  
Cristina Soares ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bloodstream Infections ◽  
Central Line ◽  
Nosocomial Pathogens ◽  
Content Type ◽  
Iron Restriction

Staphylococcus epidermidis is one of the most important nosocomial pathogens and a major cause of central line-associated bloodstream infections. Once in the bloodstream, this bacterium must surpass severe iron restriction in order to survive and establish infection.

scholarly journals Comparative Genomic and Pan-Genomic Characterization of Staphylococcus epidermidis From Different Sources Unveils the Molecular Basis and Potential Biomarkers of Pathogenic Strains

2021 ◽  
Vol 12 ◽  
Author(s):  
Shudan Lin ◽  
Bianjin Sun ◽  
Xinrui Shi ◽  
Yi Xu ◽  
Yunfeng Gu ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Ocular Trauma ◽  
Bloodstream Infections ◽  
Common Species ◽  
Comparative Genomic ◽  
Whole Genomes ◽  
Genomic Characterization ◽  
Different Sources ◽  
Potential Biomarkers

Coagulase-negative Staphylococcus (CoNS) is the most common pathogen causing traumatic endophthalmitis. Among which, Staphylococcus epidermidis is the most common species that colonizes human skin, eye surfaces, and nasal cavity. It is also the main cause of nosocomial infection, specially foreign body-related bloodstream infections (FBR-BSIs). Although some studies have reported the genome characteristics of S. epidermidis, the genome of ocular trauma-sourced S. epidermidis strain and a comprehensive understanding of its pathogenicity are still lacking. Our study sequenced, analyzed, and reported the whole genomes of 11 ocular trauma-sourced samples of S. epidermidis that caused traumatic endophthalmitis. By integrating publicly available genomes, we obtained a total of 187 S. epidermidis samples from healthy and diseased eyes, skin, respiratory tract, and blood. Combined with pan-genome, phylogenetic, and comparative genomic analyses, our study showed that S. epidermidis, regardless of niche source, exhibits two founder lineages with different pathogenicity. Moreover, we identified several potential biomarkers associated with the virulence of S. epidermidis, including essD, uhpt, sdrF, sdrG, fbe, and icaABCDR. EssD and uhpt have high homology with esaD and hpt in Staphylococcus aureus, showing that the genomes of S. epidermidis and S. aureus may have communicated during evolution. SdrF, sdrG, fbe, and icaABCDR are related to biofilm formation. Compared to S. epidermidis from blood sources, ocular-sourced strains causing intraocular infection had no direct relationship with biofilm formation. In conclusion, this study provided additional data resources for studies on S. epidermidis and improved our understanding of the evolution and pathogenicity among strains of different sources.

scholarly journals Correction to: Inhibitory effects of ultrasound irradiation on Staphylococcus epidermidis biofilm

2021 ◽  
Author(s):  
Harumi Koibuchi ◽  
Yasutomo Fujii ◽  
Yusuke Sato’o ◽  
Takashi Mochizuki ◽  
Toshiyuki Yamada ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Ultrasound Irradiation ◽  
Inhibitory Effects

scholarly journals Biofilm Formation on Tracheostomy Tubes

2002 ◽  
Vol 81 (9) ◽  
pp. 659-661 ◽  
Author(s):  
William A. Jarrett ◽  
Julie Ribes ◽  
Jose M. Manaligod
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Bacterial Biofilms ◽  
Specific Material ◽  
Tracheostomy Tubes ◽  
Implanted Devices ◽  
Scanning Electron

An increased awareness of biofilms and their mechanisms has led to a better understanding of bacterial infections that occur following the placement of tracheostomy tubes and other implanted devices and prostheses. One aspect of biofilm formation that is still subject to debate is whether the specific material that is used to manufacture a tube has any bearing in the incidence of infection. We conducted a test of four different tube materials—polyvinyl chloride, silicone, stainless steel, and sterling silver—to ascertain how bacterial biofilms form on tracheostomy tubes and to determine if there is a material-dependent difference in biofilm formation. Scanning electron microscopy demonstrated that Pseudomonas aeruginosa and Staphylococcus epidermidis both formed bacterial biofilms on tracheostomy tubes in vitro. We also found that there was no difference in susceptibility to biofilm formation among the four tube materials tested.

scholarly journals Comparative genomic and pan-genomic characteristion of Staphylococcus epidermidis from different sources unveils the molecular basis and potential biomarkers of pathogenic strains

2021 ◽  
Author(s):  
Shudan Lin ◽  
Bianjin Sun ◽  
Xinrui Shi ◽  
Yi Xu ◽  
Yunfeng Gu ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Ocular Trauma ◽  
Bloodstream Infections ◽  
Common Species ◽  
Comparative Genomic ◽  
Whole Genomes ◽  
Genomic Analyses ◽  
Different Sources ◽  
Potential Biomarkers

Coagulase-negative Staphylococcus (CoNS) is the most common pathogen causing traumatic endophthalmitis, Staphylococcus epidermidis is the most common species which colonizes human skin, eye surfaces and nasal cavity and is the main cause of nosocomial infection, specially foreign body-related bloodstream infections (FBR-BSIs). Although some studies have reported the genome characteristics of S. epidermidis, a comprehensive understanding of its pathogenicity and the genome of ocular trauma-sourced strains is still lacking. In this study, we sequenced, analyzed and reported the whole genomes of 11 ocular trauma-sourced samples of S. epidermis that caused traumatic endophthalmitis. By integrating publicly available genomes, we obtained a total of 187 S. epidermis samples from healthy and diseased eyes, skin, respiratory tract and blood. Combined with pangenome, phylogenetic and comparative genomic analyses, our study supported that S. epidermidis, regardless of niche source, exhibits two founder lineages with different pathogenicities. Moreover, we identified potential biomarkers associated with the virulence of S. epidermis, namely, essD, uhpt, sdrF, sdrG, fbe and icaABCDR. The essD and uhpt genes have high homology with esaD and hpt in Staphylococcus aureus, showing that the genomes of S. epidermidis and S. aureus may have communicated during evolution, while the sdrF, sdrG, fbe, and icaABCDR genes are related to biofilm formation. Compared to S. epidermidis from blood sources, ocular-sourced strains causing intraocular infection had no direct relationship with biofilm formation. In conclusion, this study not only provided additional data resources for studies on S. epidermidis is, but also improved understanding of the evolution and pathogenicity of different source strains.

scholarly journals Activity of Natural Polyether Ionophores: Monensin and Salinomycin against Clinical Staphylococcus epidermidis Strains

2015 ◽  
Vol 64 (3) ◽  
pp. 273-278 ◽  
Author(s):  
Joanna Stefańska ◽  
Karolina Stępień ◽  
Adam Huczyński ◽  
Stefan Tyski
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Coagulase Negative Staphylococcus ◽  
Bacterial Strains ◽  
Natural Substances ◽  
Planktonic Bacteria ◽  
Abiotic Surfaces ◽  
Extracellular Slime

Staphylococcus epidermidis, a coagulase-negative Staphylococcus, is the most important pathogen responsible for chronic nosocomial infections. These bacteria produce extracellular slime and form biofilms on various biotic and abiotic surfaces. Bacterial biofilms are very resistant to standard antimicrobial therapy and difficult to eradicate, so it is important to search for new more effective anti-biofilm agents, for example in the group of natural substances. The aim of the study was to examine the activity of two ionophores-salinomycin and monensin against clinical S. epidermidis strains, using MIC/MBC method and biofilm formation inhibition assay. Bacterial strains were tested also for slime production using Congo Red Agar. Both tested ionophore antibiotics showed the highest activity against planktonic bacteria of clinical as well as standard S. epidermidis strains and effectively inhibited the formation of bacterial biofilm.

scholarly journals Fluid Flow Induces Biofilm Formation in Staphylococcus epidermidis Polysaccharide Intracellular Adhesin-Positive Clinical Isolates

2012 ◽  
Vol 78 (16) ◽  
pp. 5890-5896 ◽  
Author(s):  
Westbrook M. Weaver ◽  
Vladana Milisavljevic ◽  
Jeff F. Miller ◽  
Dino Di Carlo
Keyword(s):  
Fluid Flow ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Hospital Costs ◽  
Bloodstream Infections ◽  
Clinical Isolates ◽  
Content Type ◽  
Biofilm Structure

ABSTRACTStaphylococcus epidermidisis a common cause of catheter-related bloodstream infections, resulting in significant morbidity and mortality and increased hospital costs. The ability to form biofilms plays a crucial role in pathogenesis; however, not all clinical isolates form biofilms under normalin vitroconditions. Strains containing theicaoperon can display significant phenotypic variation with respect to polysaccharide intracellular adhesin (PIA)-based biofilm formation, including the induction of biofilms upon environmental stress. Using a parallel microfluidic approach to investigate flow as an environmental signal forS. epidermidisbiofilm formation, we demonstrate that fluid shear alone induces PIA-positive biofilms of certain clinical isolates and influences biofilm structure. These findings suggest an important role of the catheter microenvironment, particularly fluid flow, in the establishment ofS. epidermidisinfections by PIA-dependent biofilm formation.

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