scholarly journals Shotgun metagenomics evaluation of soil fertilization effect on the rhizosphere viral community of maize plants

2021 ◽  
Author(s):  
Nwabunwanne Lilian Nwokolo ◽  
Matthew Chekwube Enebe
Keyword(s):  
Soil Fertilization ◽  
Shotgun Metagenomics ◽  
Viral Community ◽  
Fertilization Effect ◽  
Maize Plants

scholarly journals Shotgun Metagenomics evaluation of soil fertilization effect on the rhizosphere viral community of maize plants

2021 ◽  
Author(s):  
Nwabunwanne Lilian Nwokolo ◽  
MATTHEW Chekwube ENEBE
Keyword(s):  
Soil Nutrient ◽  
Fertilizer Application ◽  
Progressive Increase ◽  
Organic Manure ◽  
Inorganic Fertilizer ◽  
Soil Fertilization ◽  
Shotgun Metagenomics ◽  
Viral Community ◽  
Fertilized Soil ◽  
Abundance And Diversity

Abstract The need for sustainability in food supply has led to progressive increase in soil nutrient enrichment. Fertilizer application affect both biological and abiotic processes in the soil, of which bacterial community that support viral multiplication are equally influenced. Soil viral community composition and dynamics are affected by soil fertilization with less exploration on organic and inorganic fertilizer application. In this study, we evaluated the influence of soil fertilization on the maize rhizosphere viral community growing in Luvisolic soil. The highest abundance of bacteriophages were detected in soil treated with high compost manure (Cp8), low inorganic fertilizer (N1), low compost (Cp4) and control (Cn0). Our result showed higher frequency of Myoviridae (47%), Podoviridae (46%) and Siphoviridae (90%) in high organic manure (Cp8) fertilized compared to others. While Inoviridae (98%) and Microviridae (74%) were the most abundant phage families in low organic (Cp4) fertilized soil. This demonstrate that soil fertilization with organic manure increases the abundance and diversity of viruses in the soil due to its soil conditioning effects.

scholarly journals Foliar and soil fertilization effect on seed yield and protein content of soybean

2015 ◽  
Vol 17 (1) ◽  
pp. 67-72 ◽  
Author(s):  
MA Mannan
Keyword(s):  
Protein Content ◽  
Foliar Spray ◽  
Soybean Seed ◽  
Growth Yield ◽  
Growth And Yield ◽  
Growth Stages ◽  
Soybean Variety ◽  
Soil Fertilization ◽  
Fertilization Effect ◽  
Better Than

A field experiment was carried out at Agronomy Farm of Patuakhali Science and Technology University, Dumki, Patuakhali, from December 2011 to March 2012, to study the effects of nutrient foliar spray on soybean growth, yield and protein content. Soybean variety Shohag was used as the test crop. N, NPK, NPKS and NPKMg were sprayed and applied in the soil at vegetative and pod filling stages. Soil fertilizations were done as recommended dose, and no soil and foliar fertilization were considered as control. Plants were sprayed at the rate of 100 mg/L of water corresponding to each nutrient. The experimental design was a split plot with three replications. Result indicated that nutrient foliar spray, either singly or in combination, enhanced the growth and yield of the soybean as well as protein content in soybean seed, at the two growth stages compared to soil fertilization. However, spraying nutrients during pod filling stage was better than vegetative spraying stage in all characters studied. The highest amount of protein content in soybean seed and grain yield were obtained by spraying NPKMg.Bangladesh Agron. J. 2014, 17(1): 67-72

scholarly journals Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Matthew Chekwube Enebe ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Nitrogen Cycling ◽  
Carbon Cycling ◽  
Inorganic Fertilizer ◽  
High Dose ◽  
Soil Fertilization ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Cycling ◽  
Fertilized Soil ◽  
Catabolic Genes ◽  
Maize Plants

AbstractSoil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

scholarly journals Effect of Foliar and Soil Fertilization with New Products Based on Calcinated Bones on Selected Physiological Parameters of Maize Plants

2020 ◽  
Vol 10 (7) ◽  
pp. 2579 ◽  
Author(s):  
Natalia Matlok ◽  
Małgorzata Szostek ◽  
Piotr Antos ◽  
Grażyna Gajdek ◽  
Józef Gorzelany ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Gas Exchange ◽  
Physiological Parameters ◽  
Initial Growth ◽  
Gas Exchange Parameters ◽  
Soil Fertilization ◽  
Chlorophyll Fluorescence Parameters ◽  
Maize Plants ◽  
The Impact ◽  
Exchange Parameters

This manuscript presents the effect of foliar and soil fertilizer produced from thermally processed bone waste on the initial growth and development of maize plants. The developed fertilizers were tested in three different doses in a pot experiment. Because nutrient deficiency interferes with plant physiological processes, the impact of the developed fertilizers on gas exchange parameters, relative chlorophyll content, and chlorophyll fluorescence parameters were assessed. Based on the conducted research, it was found that fertilization with developed foliar and soil fertilizers increased the relative content of chlorophyll in maize leaves and increased the value of gas exchange parameters and chlorophyll fluorescence. All determined parameters of gas exchange and chlorophyll fluorescence showed a positive correlation depending on the dose of foliar fertilization used (average value r = 0.8414). In turn, the soil fertilization that was utilized during the experiment significantly correlated only with the content of chlorophyll (r = 0.6965). The tested fertilizers improvement of the physiological parameters of the plants, which indicates the fertilizing efficiency of the tested fertilizers.

scholarly journals Revealing the active microbiome connected with the rhizosphere soil of maize plants in Ventersdorp, South Africa

2021 ◽  
Vol 9 ◽  
Author(s):  
Olubukola Babalola ◽  
Rebaona Molefe ◽  
Adenike Amoo
Keyword(s):  
South Africa ◽  
Plant Growth ◽  
Microbial Communities ◽  
Rhizosphere Soil ◽  
Agricultural Sustainability ◽  
Shotgun Metagenomics ◽  
Maize Rhizosphere ◽  
Structural Composition ◽  
Functional Capabilities ◽  
Maize Plants

We conducted shotgun metagenomics sequencing of the maize rhizosphere and bulk soils in Ventersdorp, South Africa. Information on the structural composition and functional capabilities of microbial communities in the maize rhizosphere are provided by the data. Characterising the functional potentials of rhizosphere microbiomes gives an opportunity to link the microbiome to plant growth and health and provides the possibility of discovering new plant-beneficial genes that could enhance agricultural sustainability.

scholarly journals Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

2020 ◽  
Author(s):  
Matthew Chekwube Enebe ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Nitrogen Cycling ◽  
Carbon Cycling ◽  
Inorganic Fertilizer ◽  
Soil Fertilization ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Cycling ◽  
Fertilized Soil ◽  
Maize Plants ◽  
And Control ◽  
Compost Manure

Abstract Soil microbes performs important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this experimental study, we evaluated the fundamental effects of soil fertilization with organic (compost manure) and inorganic fertilizer on the abundances and distribution of carbon and nitrogen cycling genes within the rhizospheric regions of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do higher compost and lower inorganic fertilizer treatments. This observation was significantly different from those of higher doses of inorganic fertilizer and lower compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by lower compost and higher inorganic fertilized soil. The other treatments only selected peculiar carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control while Bacillales are promoted by low compost and higher inorganic fertilizer and this indicated that only microbes capable of tolerating the stress of higher dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lower nitrogen gas emission but increases carbon dioxide evolution in the agricultural soil.

scholarly journals Metagenomics Assessment of Soil Fertilization on the Chemotaxis and Disease Suppressive Genes Abundance in the Maize Rhizosphere

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 535
Author(s):  
Matthew Enebe ◽  
Olubukola Babalola
Keyword(s):  
Soil Fertility ◽  
Gene Diversity ◽  
Inorganic Fertilizer ◽  
Soil Fertilization ◽  
Inorganic Substances ◽  
Functional Gene Diversity ◽  
Maize Plants ◽  
High Doses ◽  
Disease Suppressive Soil ◽  
Compost Manure

Soil fertility is a function of the level of organic and inorganic substances present in the soil, and it influences the activities of soil-borne microbes, plant growth performance and a host of other beneficial ecological functions. In this metagenomics study, we evaluated the response of maize microbial functional gene diversity involved in chemotaxis, antibiotics, siderophores, and antifungals producing genes within the rhizosphere of maize plants under compost, inorganic fertilizer, and unfertilized conditions. The results show that fertilization treatments at higher compost manure and lower inorganic fertilizer doses as well as maize plants itself in the unfertilized soil through rhizosphere effects share similar influences on the abundance of chemotaxis, siderophores, antifungal, and antibiotics synthesizing genes present in the samples, while higher doses of inorganic fertilizer and lower compost manure treatments significantly repress these genes. The implication is for a disease suppressive soil to be achieved, soil fertilization with high doses of compost manure fertilizer treatments as well as lower inorganic fertilizer should be used to enrich soil fertility and boost the abundance of chemotaxis and disease suppressive genes. Maize crops also should be planted sole or intercropped with other crops to enhance the rhizosphere effect of these plants in promoting the expression and abundance of these beneficial genes in the soil.

scholarly journals Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

2021 ◽  
Author(s):  
Matthew Chekwube Enebe ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Nitrogen Cycling ◽  
Carbon Cycling ◽  
Inorganic Fertilizer ◽  
High Dose ◽  
Soil Fertilization ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Cycling ◽  
Fertilized Soil ◽  
Catabolic Genes ◽  
Maize Plants

Abstract Soil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

scholarly journals The Influence of Soil Fertilization on the Distribution and Diversity of Phosphorus Cycling Genes and Microbes Community of Maize Rhizosphere Using Shotgun Metagenomics

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1022
Author(s):  
Matthew Chekwube Enebe ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Inorganic Nitrogen ◽  
Phosphorus Cycling ◽  
Inorganic Fertilizer ◽  
Nitrogen Fertilizers ◽  
High Dose ◽  
Phosphorus Cycle ◽  
Plant Materials ◽  
Soil Fertilization ◽  
Shotgun Metagenomics ◽  
Compost Manure

Biogeochemical cycling of phosphorus in the agro-ecosystem is mediated by soil microbes. These microbes regulate the availability of phosphorus in the soil. Little is known about the response of functional traits of phosphorus cycling microbes in soil fertilized with compost manure (derived from domestic waste and plant materials) or inorganic nitrogen fertilizers at high and low doses. We used a metagenomics investigation study to understand the changes in the abundance and distribution of microbial phosphorus cycling genes in agricultural farmlands receiving inorganic fertilizers (120 kg N/ha, 60 kg N/ha) or compost manure (8 tons/ha, 4 tons/ha), and in comparison with the control. Soil fertilization with high level of compost (Cp8) or low level of inorganic nitrogen (N1) fertilizer have nearly similar effects on the rhizosphere of maize plants in promoting the abundance of genes involved in phosphorus cycle. Genes such as ppk involved in polyphosphate formation and pstSABC (for phosphate transportation) are highly enriched in these treatments. These genes facilitate phosphorus immobilization. At a high dose of inorganic fertilizer application or low compost manure treatment, the phosphorus cycling genes were repressed and the abundance decreased. The bacterial families Bacillaceae and Carnobacteriaceae were very abundant in the high inorganic fertilizer (N2) treated soil, while Pseudonocardiaceae, Clostridiaceae, Cytophagaceae, Micromonosporaceae, Thermomonosporaceae, Nocardiopsaceae, Sphaerobacteraceae, Thermoactinomycetaceae, Planococcaceae, Intrasporangiaceae, Opitutaceae, Acidimicrobiaceae, Frankiaceae were most abundant in Cp8. Pyrenophora, Talaromyces, and Trichophyton fungi were observed to be dominant in Cp8 and Methanosarcina, Methanobrevibacter, Methanoculleus, and Methanosphaera archaea have the highest percentage occurrence in Cp8. Moreover, N2 treatment, Cenarchaeum, Candidatus Nitrososphaera, and Nitrosopumilus were most abundant among fertilized soils. Our findings have brought to light the basis for the manipulation of rhizosphere microbial communities and their genes to improve availability of phosphorus as well as phosphorus cycle regulation in agro-ecosystems.

Nutrient transfer between the root zones of soybean and maize plants connected by a common mycorrhizal mycelium

1991 ◽  
Vol 82 (3) ◽  
pp. 423-432 ◽  
Author(s):  
Gabor J. Bethlenfalvay ◽  
Maria G. Reyes-Solis ◽  
Susan B. Camel ◽  
Ronald Ferrera-Cerrato
Keyword(s):  
Nutrient Transfer ◽  
Root Zones ◽  
Maize Plants
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