scholarly journals Tissue reaction after subcutaneous implantation of a membrane composed of bacterial cellulose embedded with hydroxyapatite

2015 ◽  
Vol 1 (2) ◽  
2018 ◽  
Vol 166 (2) ◽  
pp. 287-292
Author(s):  
A. S. Sotnichenko ◽  
R. Z. Nakokhov ◽  
E. A. Gubareva ◽  
E. V. Kuevda ◽  
I. S. Gumenyuk

2019 ◽  
Vol 7 (4) ◽  
pp. 630-639 ◽  
Author(s):  
Lai C. ◽  
S. J. Zhang ◽  
L. Y. Sheng ◽  
T. F. Xi

The aim of this study was to investigate the influence of poly(lactide-co-glycolide) (PLGA) and polydopamine (PDA) as coating materials on the tensile strength, surface performance, in vitro cell behavior and the in vivo material-tissue reaction of bacterial cellulose (BC) membranes.


Biomaterials ◽  
1993 ◽  
Vol 14 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Yukihiko Kinoshita ◽  
Takeshi Kuzuhara ◽  
Mitsuhiro Kirigakubo ◽  
Masaru Kobayashi ◽  
Kaizou Shimura ◽  
...  

1990 ◽  
Vol 24 (6) ◽  
pp. 689-703 ◽  
Author(s):  
K. Anselme ◽  
C. Bacques ◽  
G. Charriere ◽  
D. J. Hartmann ◽  
D. Herbage ◽  
...  

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Ismário Silva Meneses ◽  
Ricardo Luiz Cavalcanti de Albuquerque Júnior ◽  
Felipe de Souza Matos ◽  
Aline Aragão Pereira Macedo ◽  
Adriano Antunes de Souza Araújo ◽  
...  

This study aimed to analyze the tissue reaction caused by carvacrol paste associated or not with laser photobiomodulation (LPBM) at λ660 nm in the subcutaneous tissue of rats. Sixty Wistar rats were divided into four groups and they received the following interventions: subcutaneous implantation of empty polyethylene tubes (CTR), implantation of tubes containing carvacrol paste (CVC), implantation of empty tubes and LPBM (LLLT), and implantation of tubes containing carvacrol paste and LPBM (CVCLT). The animals were euthanized at three, eight, and 15 days after surgery. The inflammatory reaction and fibroplasia were analyzed histomorphometrically. Significant differences among the groups were determined by ANOVA and Tukey's test (p<0.05). In the 3-day period, the CVCLT group had low inflammatory infiltration (p<0.01). In the 8- and 15-day periods, the LLLT and CVCLT groups presented a low amount of lymphocytic inflammatory infiltrate (p<0.01 and p<0.05). Regarding the formation of fibrous tissue, the CVC group had the highest formation of type III collagen in the 8-day period (p<0.001). In the 15-day period, the CVCLT group had a lower formation of type I collagen than the CTR and LLLT groups (p<0.05). The use of the carvacrol paste associated with photobiomodulation optimizes the inflammatory period and tissue repair.


2019 ◽  
Vol 88 (4) ◽  
pp. 451-471 ◽  
Author(s):  
Ioana Maria Bodea ◽  
Giorgiana Mihaela Cătunescu ◽  
Teodor Florian Stroe ◽  
Sonia Alexandra Dîrlea ◽  
Florin Ioan Beteg

Tissue engineering promotes tissue regeneration through biomaterials that have excellent properties and have the potential to replace tissues. Many studies show that bacterial cellulose (BC) might ensure tissue regeneration and substitution, being used for the bioengineering of hard, cartilaginous and soft tissues. Bacterial cellulose is extensively used as wound dressing material and results show that BC is a promising tissue scaffold (bone, cardiovascular, urinary tissue). It can be combined with polymeric and non-polymeric compounds to acquire antimicrobial, cell-adhesion and proliferation properties. To ensure proper tissue regeneration, the material has to be: biocompatible, with minimum tissue reaction and biodegradability; bio-absorbable, to promote tissue development, cellular interaction and grow; resistant to support the weight of the newly formed tissue. Its versatile structure, physical and biochemical properties can be adjusted by adapting the bacteria culturing conditions. The main biomedical applications seem to be as hard (bone, dental), fibrocartilaginous (meniscal) and soft tissue (skin, cardiovascular, urinary) substituents. This paper reviews the current state of knowledge, challenges and future applications of BC and its biomedical potential in veterinary medicine. It was focused on the main uses in regeneration and scaffold tissue replacement and, although BC showed promising results, there is a lack of successful results of BC use in clinical practice. Most studies were performed only at experimental level and further research is needed for BC to enter clinical veterinary practice.


2011 ◽  
Vol 22 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Esther Ruiz De Castañeda ◽  
Léa A. B. Silva ◽  
Patrícia Gaton-Hernández ◽  
Alberto Consolaro ◽  
Encarnación Gonzalez Rodriguez ◽  
...  

The aim of this study was to evaluate the tissue compatibility of a silorane-based resin system (FiltekTM Silorane) and a methacrylate-based nanoparticle resin (FiltekTM Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice. One hundred and thirty five male isogenic BALB/c mice were randomly assigned to 12 experimental and 3 control groups, according to the implanted material and the experimental period of 7, 21 and 63 days. At the end of each period, the animals were killed and the tubes with the surrounding tissues were removed and processed for microscopic analysis. Samples were subjected to a descriptive and a semi-quantitative analyses using a 4-point scoring system (0-3) to evaluate the collagen fiber formation and inflammatory infiltrate. Data were statistically analyzed using the Kruskal Wallis test (?=0.05). The results showed that there was no significant difference between the experimental and control groups considering the three evaluation periods (p>0.05). The silorane-based and the methacrylate-based nanoparticle resins presented similar tissue response to that of the empty tube (control group) after subcutaneous implantation in isogenic mice.


2021 ◽  
Vol 22 (14) ◽  
pp. 7623
Author(s):  
Ignacio Stöwe ◽  
Jens Pissarek ◽  
Pia Moosmann ◽  
Annica Pröhl ◽  
Sven Pantermehl ◽  
...  

(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus was to analyze the (ultra-) structure and the purification effort. (2) Methods: Established methodologies such as the histological material analysis and the conduct of the subcutaneous implantation model in Wistar rats were applied. Moreover, established methods combining histological, immunohistochemical, and histomorphometrical procedures were applied to analyze the tissue reactions to the vessel graft materials, including the induction of pro- and anti-inflammatory macrophages to test the immune response. (3) Results: The results showed that the PAP implants induced a special cellular infiltration and host tissue integration based on its three different parts based on the different layers of the donor tissue. Thereby, these material parts induced a vascularization pattern that branches to all parts of the graft and altogether a balanced immune tissue reaction in contrast to the control material. (4) Conclusions: PAP implants seemed to be advantageous in many aspects: (i) cellular infiltration and host tissue integration, (ii) vascularization pattern that branches to all parts of the graft, and (iii) balanced immune tissue reaction that can result in less scar tissue and enhanced integrative healing patterns. Moreover, the unique trans-implant vascularization can provide unprecedented anti-infection properties that can avoid material-related bacterial infections.


Author(s):  
G.E. Visscher ◽  
R. L. Robison ◽  
G. J. Argentieri

The use of various bioerodable polymers as drug delivery systems has gained considerable interest in recent years. Among some of the shapes used as delivery systems are films, rods and microcapsules. The work presented here will deal with the techniques we have utilized for the analysis of the tissue reaction to and actual biodegradation of injectable microcapsules. This work has utilized light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques. The design of our studies has utilized methodology that would; 1. best characterize the actual degradation process without artifacts introduced by fixation procedures and 2. allow for reproducible results.In our studies, the gastrocnemius muscle of the rat was chosen as the injection site. Prior to the injection of microcapsules the skin above the sites was shaved and tattooed for later recognition and recovery. 1.0 cc syringes were loaded with the desired quantity of microcapsules and the vehicle (0.5% hydroxypropylmethycellulose) drawn up. The syringes were agitated to suspend the microcapsules in the injection vehicle.


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