3D printed transwell-integrated nose-on-chip model to evaluate effects of air flow-induced mechanical stresses on mucous secretion

2022 ◽  
Vol 24 (1) ◽  
Author(s):  
Zachary Brooks ◽  
Kanghyun Kim ◽  
Kai Zhao ◽  
Tarun Goswami ◽  
Saber Hussain ◽  
...  
Keyword(s):  
Air Flow ◽  
Mechanical Stresses ◽  
Mucous Secretion ◽  
3D Printed ◽  
On Chip

scholarly journals 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
John-Alexander Preuss ◽  
Janina Bahnemann ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Single Cells ◽  
Lab On A Chip ◽  
Cost Effective ◽  
Optical Manipulation ◽  
Hydrodynamic Focusing ◽  
Trapping Region ◽  
Dual Beam ◽  
3D Printed ◽  
On Chip

Abstract3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.

scholarly journals Epitaxial Graphene Sensors Combined with 3D-Printed Microfluidic Chip for Heavy Metals Detection

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2393 ◽  
Author(s):  
Maria Francesca Santangelo ◽  
Ivan Shtepliuk ◽  
Daniel Filippini ◽  
Donatella Puglisi ◽  
Mikhail Vagin ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Density Functional ◽  
Topological Analysis ◽  
Decomposition Analysis ◽  
Epitaxial Graphene ◽  
Phase Detection ◽  
Lab On Chip ◽  
Covalent Interaction ◽  
3D Printed ◽  
On Chip

In this work, we investigated the sensing performance of epitaxial graphene on Si-face 4H-SiC (EG/SiC) for liquid-phase detection of heavy metals (e.g., Pb and Cd), showing fast and stable response and low detection limit. The sensing platform proposed includes 3D-printed microfluidic devices, which incorporate all features required to connect and execute lab-on-chip (LOC) functions. The obtained results indicate that EG exhibits excellent sensing activity towards Pb and Cd ions. Several concentrations of Pb2+ solutions, ranging from 125 nM to 500 µM, were analyzed showing Langmuir correlation between signal and Pb2+ concentrations, good stability, and reproducibility over time. Upon the simultaneous presence of both metals, sensor response is dominated by Pb2+ rather than Cd2+ ions. To explain the sensing mechanisms and difference in adsorption behavior of Pb2+ and Cd2+ ions on EG in water-based solutions, we performed van-der-Waals (vdW)-corrected density functional theory (DFT) calculations and non-covalent interaction (NCI) analysis, extended charge decomposition analysis (ECDA), and topological analysis. We demonstrated that Pb2+ and Cd2+ ions act as electron-acceptors, enhancing hole conductivity of EG, due to charge transfer from graphene to metal ions, and Pb2+ ions have preferential ability to binding with graphene over cadmium. Electrochemical measurements confirmed the conductometric results, which additionally indicate that EG is more sensitive to lead than to cadmium.

scholarly journals 3D Printed Electrophoretic Lab-on-chip for DNA Separation

2016 ◽  
Vol 168 ◽  
pp. 1454-1457 ◽  
Author(s):  
K. Adamski ◽  
W. Kubicki ◽  
R. Walczak
Keyword(s):  
Dna Separation ◽  
Lab On Chip ◽  
3D Printed ◽  
On Chip

scholarly journals 3D Printed Microfluidic Devices for Solid-Phase Extraction and On-Chip Fluorescent Labeling of Preterm Birth Risk Biomarkers

2020 ◽  
Vol 92 (18) ◽  
pp. 12322-12329 ◽  
Author(s):  
Anna V. Bickham ◽  
Chao Pang ◽  
Benjamin Q. George ◽  
David J. Topham ◽  
Jacob B. Nielsen ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Microfluidic Devices ◽  
Fluorescent Labeling ◽  
Phase Extraction ◽  
3D Printed ◽  
On Chip ◽  
Risk Biomarkers

Inkjet 3D printed modular microfluidic chips for on-chip gel electrophoresis

2019 ◽  
Vol 29 (5) ◽  
pp. 057001 ◽  
Author(s):  
Rafał Walczak ◽  
Krzysztof Adamski ◽  
Wojciech Kubicki
Keyword(s):  
Gel Electrophoresis ◽  
Microfluidic Chips ◽  
3D Printed ◽  
On Chip

scholarly journals Epitaxial Graphene Sensors Combined with 3D Printed Microfluidic Chip for Heavy Metals Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 982 ◽  
Author(s):  
Maria Francesca Santangelo ◽  
Ivan Shtepliuk ◽  
Donatella Puglisi ◽  
Daniel Filippini ◽  
Rositsa Yakimova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Microfluidic Chip ◽  
Chemical Interaction ◽  
High Sensitivity ◽  
Epitaxial Graphene ◽  
Phase Detection ◽  
Lab On Chip ◽  
Sensing Platform ◽  
3D Printed ◽  
On Chip

Two-dimensional materials may constitute key elements in the development of a sensing platform where extremely high sensitivity is required, since even minimal chemical interaction can generate appreciable changes in the electronic state of the material. In this work, we investigate the sensing performance of epitaxial graphene on Si-face 4H-SiC (EG/SiC) for liquid-phase detection of heavy metals (e.g., Pb). The integration of preparatory steps needed for sample conditioning is included in the sensing platform, exploiting fast prototyping using a 3D printer, which allows direct fabrication of a microfluidic chip incorporating all the features required to connect and execute the Lab-on-chip (LOC) functions. It is demonstrated that interaction of Pb2+ ions in water-based solutions with the EG enhances its conductivity exhibiting a Langmuir correlation between signal and Pb2+ concentration. Several concentrations of Pb2+ solutions ranging from 125 nM to 500 µM were analyzed showing good stability and reproducibility over time.

Effect of Corner Underfill Voids on Chip Scale Package (CSP) Performance Under Mechanical Loading

2002 ◽  
Author(s):  
Sridhar Canumalla ◽  
Santosh Shetty ◽  
Nael Hannan
Keyword(s):  
Numerical Simulation ◽  
Solder Joint ◽  
Mechanical Loading ◽  
Critical Role ◽  
Mechanical Stresses ◽  
Chip Scale Package ◽  
Simulation Results ◽  
On Chip

Abstract The critical impact of corner voids on the anticipated reliability of CSP subjected to mechanical stresses is demonstrated in this paper. Experimental and numerical simulation results indicate that the presence of a corner underfill void subjects the solder joint to stress values approaching that of a non-underfilled CSP. In the absence of underfill voids, the reliability in drop testing improved when the CSP was underfilled, and the improvement was inversely proportional to the modulus of the underfill in the range studied. Results indicate that underfill quality could potentially play a critical role in determining reliability in the field for products subjected to mechanical loading.

Three-dimensional simulation and experimental investigation of three-dimensional printed guiding devices on lattice-apron compact spinning

2020 ◽  
pp. 004051752098258
Author(s):  
Malik YH Saty ◽  
Nicholus Tayari Akankwasa ◽  
Jun Wang
Keyword(s):  
Experimental Investigation ◽  
Air Flow ◽  
Flow Analysis ◽  
Three Dimensional ◽  
Flow Characteristics ◽  
Yarn Properties ◽  
3D Printed ◽  
Dimensional Simulation ◽  
Compact Spinning ◽  
Set Up

The compact spinning system with a lattice apron utilizes air-flow dynamics to condense fibers in a bunch and enhance the yarn properties. One of the main challenges with this method is the lack of a comprehensive understanding of the air-flow field's effect in the condensing zone. This work presents a numerical and experimental investigation of the effects of three-dimensional (3D) printed guiding devices on the air-flow characteristics and yarn properties. Firstly, the 3D numerical model of the compact spinning system was set up based on the compact spinning machine geometrical dimensions. Secondly, different 3D prototypes were developed, simulated, and analyzed using computational fluid dynamics based on ANSYS software. The prototypes (A-type, B-type, and C-type), selected according to the simulation results, were then 3D printed to enable further experimental investigation. Air-flow analysis results in the air-suction flume area exhibiting a very high negative pressure, and the centerline zone was characterized by high velocity. Experimental results revealed that the three yarns spun with guiding devices had better strength, hairiness, and evenness than those spun without a guiding device. The model developed can be further improved and utilized for commercial purposes and is anticipated to improve compact spun yarn properties significantly.

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