Intraindividual variability over time in plasma biomarkers of inflammation and effects of long-term storage

Many radioactive material shipping packages incorporate cane fiberboard overpacks for thermal insulation and impact resistance. Mechanical, thermal and physical properties have been measured on cane fiberboard following thermal aging in several temperature/humidity environments. Several of the measured properties change significantly over time in the more severe environments, while other properties are relatively constant. These properties continue to be tracked, with the goal of developing a model for predicting a service life under long-term storage conditions.

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Effect of Long-term Storage on Sterile Status of Devices in Surgical Packs

Infection Control ◽

10.1017/s0195941700066261 ◽

1987 ◽

Vol 8 (7) ◽

pp. 289-293 ◽

Cited By ~ 21

Author(s):

N. Ariene Klapes ◽

Velvl W. Greene ◽

Ann C. Langholz ◽

Cindy Hunstiger

Keyword(s):

Stainless Steel ◽

Laminar Flow ◽

Long Term Storage ◽

Laminar Flow Hood ◽

Storage Times ◽

And Storage ◽

Term Storage ◽

Over Time

AbstractWe investigated the effect of the following on the sterile integrity of surgical packs: four wrapping materials (two-ply reusable, nonbarrier wovens, both new and previously used; disposable, barrier nonwovens; and polypropylene peel pouches), dustcovers, two storage locations, and storage times ranging from 2 to 50 weeks. Two hundred sixty-three packs containing stainless steel coupons were prepared, wrapped, sterilized, and stored. Half of the packs were dustcovered prior to storage. At monthly intervals for a year, packs of each type were opened in a laminar flow hood, and the coupons inoculated into trypticase soy broth. The coupon contamination probabilities were 0.019 for reusable, woven packs; 0.017 for disposable, nonwoven packs; and 0.016 for peel pouches. These differences were not significant. The probability of finding a contaminated coupon in any pack after 50 weeks was 0.018. No trend toward increased probability of contamination over time was observed for any of the pack types studied.

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Effect of ageing on magnetic and electrical properties of Ti–substituted La-Sr manganites

EPJ Web of Conferences ◽

10.1051/epjconf/201818506002 ◽

2018 ◽

Vol 185 ◽

pp. 06002 ◽

Cited By ~ 1

Author(s):

A. Badelin ◽

Z. Datskaya ◽

S. Estemirova ◽

V. Karpasyuk ◽

D. Merkulov

Keyword(s):

Curie Point ◽

Storage Period ◽

X Ray Diffraction ◽

Electrical Parameters ◽

Long Term Storage ◽

Ti Content ◽

Term Storage ◽

Over Time ◽

Error Of Measurement

The changes of structural, magnetic and electrical parameters of ceramic La0.8-xSr0.2+xMn1-xTixO3+γ (0.025 ≤ x ≤ 0.150) manganites during long-term storage at room temperature and after thermal cycling were investigated. The unit cell volume (V) of all manganites decreased over time. Most considerable decrease of V and broadening of X-ray diffraction lines width were observed in the samples with high quantity of titanium. During the storage period magnetization (σ) and Curie point (Tc) of manganites with relatively low Ti content decreased, while the compositions with high values of “x” exhibited some trend of σ and Tc increase. The resistance of the samples with x < 0.100 increased over time. Fluctuations of magnetization of all thermal-aged samples were within the error of measurement, and Curie point of manganites with x = 0.150 showed the rise. From the data obtained it might be inferred that ageing phenomena in Ti-substituted manganites can be associated with oxidation processes, the transfer of Ti4+ to vacant sites in rare-earth sublattice and formation of microinhomogeneities.

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Quality assessment of RNA in long-term storage: The All Our Families biorepository

PLoS ONE ◽

10.1371/journal.pone.0242404 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0242404

Author(s):

Nikki L. Stephenson ◽

Kylie K. Hornaday ◽

Chelsea T. A. Doktorchik ◽

Andrew W. Lyon ◽

Suzanne C. Tough ◽

...

Keyword(s):

Population Based ◽

Rate Of Change ◽

Population Based Study ◽

Acid Yield ◽

Rna Integrity ◽

Long Term Storage ◽

Term Storage ◽

Over Time

Background The All Our Families (AOF) cohort study is a longitudinal population-based study which collected biological samples from 1948 pregnant women between May 2008 and December 2010. As the quality of samples can decline over time, the objective of the current study was to assess the association between storage time and RNA (ribonucleic acid) yield and purity, and confirm the quality of these samples after 7–10 years in long-term storage. Methods Maternal whole blood samples were previously collected by trained phlebotomists and stored in four separate PAXgene Blood RNA Tubes (PreAnalytiX) between 2008 and 2011. RNA was isolated in 2011 and 2018 using PAXgene Blood RNA Kits (PreAnalytiX) as per the manufacturer’s instruction. RNA purity (260/280), as well as RNA yield, were measured using a Nanodrop. The RNA integrity number (RIN) was also assessed from 5–25 and 111–130 months of storage using RNA 6000 Nano Kit and Agilent 2100 BioAnalyzer. Descriptive statistics, paired t-test, and response feature analysis using linear regression were used to assess the association between various predictor variables and quality of the RNA isolated. Results Overall, RNA purity and yield of the samples did not decline over time. RNA purity of samples isolated in 2011 (2.08, 95% CI: 2.08–2.09) were statistically lower (p<0.000) than samples isolated in 2018 (2.101, 95% CI: 2.097, 2.104), and there was no statistical difference between the 2011 (13.08 μg /tube, 95% CI: 12.27–13.89) and 2018 (12.64 μg /tube, 95% CI: 11.83–13.46) RNA yield (p = 0.2964). For every month of storage, the change in RNA purity is -0.01(260/280), and the change in RNA yield between 2011 and 2018 is -0.90 μ g / tube. The mean RIN was 8.49 (95% CI:8.44–8.54), and it ranged from 7.2 to 9.5. The rate of change in expected RIN per month of storage is 0.003 (95% CI 0.002–0.004), so while statistically significant, these results are not relevant. Conclusions RNA quality does not decrease over time, and the methods used to collect and store samples, within a population-based study are robust to inherent operational factors which may degrade sample quality over time.

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Long term storage of miRNA at room and elevated temperatures in a silica sol–gel matrix

RSC Advances ◽

10.1039/d1ra04719a ◽

2021 ◽

Vol 11 (50) ◽

pp. 31505-31510

Author(s):

Rajat Chauhan ◽

Theodore S. Kalbfleisch ◽

Chinmay S. Potnis ◽

Meenakshi Bansal ◽

Mark W. Linder ◽

...

Keyword(s):

Room Temperature ◽

Elevated Temperatures ◽

Sol Gel ◽

Silica Sol ◽

Rapid Degradation ◽

Global Impact ◽

Long Term Storage ◽

Term Storage ◽

Over Time

Storage of biospecimens in their near native environment at room temperature can have a transformative global impact, however, this remains an arduous challenge to date due to the rapid degradation of biospecimens over time.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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