scholarly journals Stripe rust resistance genes in a set of Ethiopian bread wheat cultivars and breeding lines

Euphytica ◽  
2020 ◽  
Vol 216 (2) ◽  
Author(s):  
Zeray Siyoum Gebreslasie ◽  
Shuo Huang ◽  
Gangming Zhan ◽  
Ayele Badebo ◽  
Qingdong Zeng ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Shaanxi Province ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Wheat Lines

AbstractStripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one the most important diseases of wheat in Ethiopia and worldwide. To identify resistance genes, 90 bread wheat lines and 10 cultivars were tested at the seedling stage against one Pst race from Ethiopia and six races from China as well as evaluated for the stripe rust response in an inoculated field nursery at Yangling, Shaanxi province and in a naturally infected field in Jiangyou, Sichuan, China. Resistance genes were postulated using molecular assays for Yr9, Yr17, Yr18, Yr26, Yr29, Yr36, Yr44 and Yr62. Of the 100 entries tested, 16 had all stage resistance to all races. Molecular markers were positive for Yr9 in five genotypes, Yr17 in 21 genotypes, Yr18 in 27 genotypes, Yr26 in ten genotypes, Yr29 in 22 genotypes, Yr36 in 12 genotypes, Yr44 in 30 genotypes, and Yr62 in 51 genotypes. No line had Yr5, Yr8, Yr10 or Yr15. Complete or all stage resistance was observed in genotypes carrying gene combinations Yr9 + Yr18 + Yr44 + Yr62, Yr29 + Yr62 + Yr26 and Yr9 + Yr17 + Yr26 + Yr44 + Yr62. The results are helpful for developing wheat cultivars with effective and more durable resistance to stripe rust both in China and Ethiopia.

Identification of Stripe Rust Resistance Loci in U.S. Spring Wheat Cultivars and Breeding Lines Using Genome-Wide Association Mapping and Yr Gene Markers

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2181-2192 ◽  
Author(s):  
Lu Liu ◽  
Meinan Wang ◽  
Zhiwu Zhang ◽  
Deven R. See ◽  
Xianming Chen
Keyword(s):  
Stripe Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
The United States ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Genome Wide

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a major threat to wheat production worldwide, especially in the United States. To identify loci for effective stripe rust resistance in U.S. wheat, a genome-wide association study (GWAS) was conducted using a panel of 616 spring wheat cultivars and breeding lines. The accessions in this panel were phenotyped for stripe rust response in the greenhouse at seedling stage with five predominant and highly virulent races of Pst and in different field environments at adult-plant stage in 2017 and 2018. In total, 2,029 single-nucleotide polymorphism markers that cover the whole genome were generated with genotyping by multiplexed sequencing and used in GWAS. In addition, 23 markers of previously reported resistance genes or quantitative trait loci (QTLs) were used to genotype the population. This spring panel was grouped into three subpopulations based on principal component analysis. A total of 37 genes or QTLs including 10 potentially new QTLs for resistance to stripe rust were detected by GWAS and linked marker tests. The frequencies of the resistance genes or QTLs in various nurseries were determined, indicating different intensities of these genes or QTLs used in breeding programs of different regions. These resistance loci and the information on their markers, effectiveness, and distributions should be useful for improving stripe rust resistance in wheat cultivars.

scholarly journals Development, Validation, and Re-selection of Wheat Lines with Pyramided Genes Yr64 and Yr15 Linked on the Short Arm of Chromosome 1B for Resistance to Stripe Rust

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Yanmin Qie ◽  
Yan Liu ◽  
Meinan Wang ◽  
Xing Li ◽  
Deven R. See ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Genotype Data ◽  
Wheat Cultivars ◽  
Wheat Line ◽  
Individual Gene ◽  
High Level ◽  
Wheat Lines ◽  
Selection Of

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. The disease is most preferably managed by developing and growing cultivars with high-level, durable resistance. To achieve high-level and long-lasting resistance, we developed a wheat line, RIL-Yr64/Yr15, by pyramiding Yr64 and Yr15, both on the chromosome 1BS and providing high resistance to all tested Pst races. To validate RIL-Yr64/Yr15 possessing both genes, we crossed it to Avocet S (AvS). The F4 RILs from this cross were phenotyped with Pst races under controlled greenhouse conditions and also under natural Pst infection in the field. The population was genotyped with SSR markers previously reported to be linked to the resistance gene loci and with additional SSR and SNP-KASP markers along chromosome 1B. Both phenotype and genotype data confirmed the copresence of Yr64 and Yr15 in RIL-Yr64/Yr15, and the high-resolution linkage map dissected the chromosomal regions and traced their origins. New lines possessing these genes were selected from the F5 population of cross AvS × RIL-Yr64/Yr15 by marker-assisted selection. These lines with the two highly effective genes should be more useful than individual gene lines for developing high-level, durable resistant wheat cultivars.

scholarly journals Effective Resistance to Wheat Stripe Rust in a Region with High Disease Pressure

Plant Disease ◽  
2014 ◽  
Vol 98 (7) ◽  
pp. 891-897 ◽  
Author(s):  
B. Bai ◽  
J. Y. Du ◽  
Q. L. Lu ◽  
C. Y. He ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Hot Spot ◽  
Infection Type ◽  
Durable Resistance ◽  
Gansu Province ◽  
Stripe Rust Resistance ◽  
Adult Plant

Stripe rust is a major fungal disease of wheat. It frequently becomes epidemic in southeastern Gansu province, a stripe rust hot spot in China. Evaluations of wheat germplasm response are crucial for developing cultivars to control the disease. In total, 57 wheat cultivars and lines from Europe and other countries, comprising 36 cultivars with documented stripe rust resistance genes and 21 with unknown genes, were tested annually with multiple races of Puccinia striiformis f. sp. tritici in the field at Tianshui in Gansu province from 1993 to 2013. Seven wheat lines were highly resistant, with infection type (IT) 0 during the entire period; 16 were moderately resistant (IT 0;-2); and 26 were moderately susceptible (IT 0;-4), with low maximum disease severity compared with the susceptible control Huixianhong. ‘Strampelli’ and ‘Libellula’, with three and five quantitative trait loci, respectively, for stripe rust resistance have displayed durable resistance in this region for four decades. Ten cultivars, including ‘Lantian 15’, ‘Lantian 26’, and ‘Lantian 31’, with stripe rust resistance derived from European lines, were developed in our breeding program and have made a significant impact on controlling stripe rust in southeastern Gansu. Breeding resistant cultivars with multiple adult-plant resistance genes seems to be a promising strategy in wheat breeding for managing stripe rust in this region and other hot spots.

scholarly journals Seedling and Slow Rusting Resistance to Stripe Rust in Chinese Common Wheats

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1302-1312 ◽  
Author(s):  
Z. F. Li ◽  
X. C. Xia ◽  
X. C. Zhou ◽  
Y. C. Niu ◽  
Z. H. He ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Gene Pyramiding ◽  
Stripe Rust Resistance ◽  
Slow Rusting ◽  
Cropping Seasons ◽  
Wheat Lines ◽  
Rust Resistance Genes

Identification of seedling and slow stripe rust resistance genes is important for gene pyramiding, gene deployment, and developing slow-rusting wheat cultivars to control the disease. A total of 98 Chinese lines were inoculated with 26 pathotypes of Puccinia striiformis f. sp. tritici for postulation of stripe rust resistance genes effective at the seedling stage. A total of 135 wheat lines were planted at two locations to characterize their slow rusting responses to stripe rust in the 2003-2004 and 2004-2005 cropping seasons. Genes Yr2, Yr3a, Yr4a, Yr6, Yr7, Yr9, Yr26, Yr27, and YrSD, either singly or in combinations, were postulated in 72 lines, whereas known resistance genes were not identified in the other 26 accessions. The resistance genes Yr9 and Yr26 were found in 42 and 19 accessions, respectively. Yr3a and Yr4a were detected in two lines, and four lines may contain Yr6. Three lines were postulated to possess YrSD, one carried Yr27, and one may possess Yr7. Thirty-three lines showed slow stripe rusting resistance at two locations in both seasons.

scholarly journals Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

2016 ◽  
Vol 40 (3) ◽  
pp. 279-287 ◽  
Author(s):  
Hassan Mahmoud Emara ◽  
Ayman Feisal Omar ◽  
Moustafa Mahmoud El-Shamy ◽  
Mona Elsaid Mohamed
Keyword(s):  
Powdery Mildew ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Nile Delta ◽  
Durable Resistance ◽  
Wheat Cultivars ◽  
Wheat Diseases ◽  
Environmentally Safe ◽  
Wheat Lines

ABSTRACT Powdery mildew of wheat (Triticum spp.) caused by Blumeria graminis f.sp. tritici (DC) E.O. Speer Em. Marchal is one of the most important bread wheat diseases in Egypt. All the Egyptian common bread wheat cultivars are susceptible to that disease at seedling and adult stages. Breeding of resistant cultivars is the most economical and environmentally safe method to eliminate the disease and reduce crop losses. Combinations of two or more effective resistance genes may lead to better, more durable resistance to that disease. Eight Pm genes i.e. Pm2, Pm6, Pm12, Pm16, Pm24, Pm35, Pm36 and Pm37 out of 21 powdery mildew monogenic wheat lines (Pm) were resistant to 42 individual isolates of powdery mildew collected from different governorates in the Nile Delta area, Egypt, at seedling and adult stages. Only four DNA specific SSR markers (Xgwm337, Xcfd7 linked to Pm24, Pm35 and Xgwm332, Xwmc790) linked to Pm37 resistance genes were selected to detect these genes in 13 Egyptian common bread wheat cultivars. This study reveals the absence of Pm24, Pm35 and Pm37 in all the 13 Egyptian bread wheat cultivars. These results gave evidence that the Egyptian cultivars are not having resistance genes and need to further incorporate one, two or more resistant genes in a single genotype as all commercial cultivars defeated by the pathogen.

scholarly journals Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars From the Huang-Huai-Hai Region of China

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1763-1770
Author(s):  
Liang Huang ◽  
Xing Zhi Xiao ◽  
Bo Liu ◽  
Li Gao ◽  
Guo Shu Gong ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Molecular Testing ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Yr Genes

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

Genome-wide Wheat 55K SNP-based Mapping of Stripe Rust Resistance Loci in Wheat Cultivar Shaannong 33 and Their Alleles Frequencies in Current Chinese Wheat Cultivars and Breeding Lines

Plant Disease ◽  
2020 ◽  
Author(s):  
Shuo Huang ◽  
Shengjie Liu ◽  
Yibo Zhang ◽  
Yanzhou Xie ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Seedling Resistance ◽  
Resistant Parent ◽  
Breeding Lines ◽  
Chinese Wheat

Wheat cultivar Shannong 33 (SN33) has remained highly resistant to stripe rust in the field since its release in 2009. To unravel the genetic architecture of stripe rust resistance, seedlings of 161 recombinant inbred lines (RILs) from the cross Avocet S × SN33 were evaluated with two isolates (PST-Lab.1 and PST-Lab.2) of the stripe rust pathogen (Puccinia striiformis f. sp. tritici) in the greenhouse, and the RILs were evaluated in naturally and/or artificially inoculated field sites during two cropping seasons. The RILs and parents were genotyped with the wheat 55K single nucleotide polymorphism (SNP) array. Three genomic regions conferring seedling resistance were mapped on chromosomes 1DS, 2AS, and 3DS, and four consistent quantitative trait loci (QTL) for adult-plant resistance (APR) were detected on 1BL, 2AS, 3DL, and 6BS. The 2AS locus conferring all-stage resistance was identified as the resistant gene Yr17 located on 2NS translocation. The QTL identified on 1BL and 6BS likely correspond to Yr29 and Yr78, respectively. An APR QTL on 3DL explaining 5.8–12.2% of the phenotypic variation is likely to be new. Molecular marker detection assays with the 2NS segment (Yr17), Yr29, Yr78, and QYrsn.nwafu-3DL on a panel of 420 current Chinese wheat cultivars and breeding lines indicated that these genes were present in 11.4%, 7.6%, 14.8%, and 7.4% entries, respectively. The interactions among these genes/QTL were additive suggesting their potential value in enhancing stripe rust resistance breeding materials as observed in the resistant parent. In addition, we also identified two leaf necrosis genes, Ne1 and Ne2, however, the F1 plants from cross Avocet S × SN33 survived indicating that SN33 probably has another allele of Ne1 which allows to harvest seeds.

scholarly journals Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China

Plant Disease ◽  
2015 ◽  
Vol 99 (6) ◽  
pp. 754-760 ◽  
Author(s):  
D. J. Han ◽  
Q. L. Wang ◽  
X. M. Chen ◽  
Q. D. Zeng ◽  
J. H. Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Sichuan Basin ◽  
Puccinia Striiformis ◽  
Field Tests ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Breeding Lines ◽  
Wheat Production ◽  
Stripe Rust Resistance Gene ◽  
The Sichuan Basin

Stripe rust, caused by Puccinia striiformis f. tritici, is one of the most destructive diseases of wheat in the world. The Sichuan Basin is one of the most important regions of wheat production and stripe rust epidemics in China. Stripe rust resistance gene Yr26 (the same gene as Yr24) has been widely used in wheat breeding programs and in many cultivars grown in this region since the gene was discovered in the early 1990s. Virulence to Yr26 has increased in frequency since its first detection in 2008. The objective of this study was to assess the vulnerability of the wheat cultivars and breeding lines in the Sichuan Basin to Yr26-virulent races. In total, 85 wheat accessions were tested with Yr26-avirulent races CYR32, CYR33, and Su11-4 and two Yr26-virulent races, V26/CM42 and V26/Gui22. DNA markers for Yr26 were used to determine the presence and absence of Yr26 in the wheat accessions. Of the 85 wheat accessions, only 5 were resistant and 19 susceptible to all races tested, and the remaining 61 were resistant to at least one or more races tested in seedling stage. In all, 65 (76.5%) accessions were susceptible to the emerging Yr26-virulent race V26/Gui22. In field tests, susceptible accessions increased from 31.8% in a nursery inoculated with predominant and Yr26-avirulent races to 61.2% in the nursery inoculated with the predominant races mixed with V26/Gui22. Based on the results of the molecular marker and race tests, 33 (38.8%) accessions were determined to have Yr26, showing that the Yr26 virulence is a major threat to wheat production in the Sichuan Basin and potentially in other regions of China.

Sign in / Sign up

Export Citation Format

Share Document