Memory regulation in feeding habit transformation to dead prey fish of Chinese perch (Siniperca chuatsi)

2021 ◽  
Author(s):  
Linjie Shi ◽  
Jiao Li ◽  
Xu-Fang Liang ◽  
Shan He ◽  
Yaqi Dou ◽  
...  
Keyword(s):  
Feeding Habit ◽  
Prey Fish ◽  
Siniperca Chuatsi ◽  
Chinese Perch ◽  
Dead Prey

Feeding habit transition induced by social learning through CaMKII signaling in Chinese perch (Siniperca chuatsi)

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736211
Author(s):  
Hui Liang ◽  
Shan He ◽  
Xu-Fang Liang ◽  
Hai-Lin Lu ◽  
Ke Chen
Keyword(s):  
Social Learning ◽  
Feeding Habit ◽  
Siniperca Chuatsi ◽  
Chinese Perch

scholarly journals Social Learning of Acquiring Novel Feeding Habit in Mandarin Fish (Siniperca chuatsi)

2019 ◽  
Vol 20 (18) ◽  
pp. 4399 ◽  
Author(s):  
Jian Peng ◽  
Ya-Qi Dou ◽  
Hui Liang ◽  
Shan He ◽  
Xu-Fang Liang ◽  
...  
Keyword(s):  
Social Learning ◽  
Protein Kinase ◽  
Food Intake ◽  
Feeding Habits ◽  
Feeding Habit ◽  
Prey Fish ◽  
Mandarin Fish ◽  
Live Prey ◽  
Learning Group ◽  
Dead Prey

Social learning plays important roles in gaining new foraging skills and food preferences. However, the potential role and molecular mechanism of social learning in acquiring new feeding habits is less clear in fish. In the present study, we examined the success rate of feeding habit domestication from live prey fish to dead prey fish, as well as the food intake of dead prey fish in mandarin fish with or without feeders of dead prey fish as demonstrators. Here, we found that mandarin fish can learn from each other how to solve novel foraging tasks, feeding on dead prey fish. In addition, the analysis of gene expressions and signaling pathways of learning through Western blotting and transcriptome sequencing shows that the expression of the c-fos, fra2, zif268, c/ebpd and sytIV genes were significantly increased, and the anorexigenic pomc and leptin a expressions were decreased in fish of the learning group. The phosphorylation levels of protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) in the learning group were significantly higher than those of the control group, while the phosphorylation level of S6 ribosomal protein (S6) was lower. With the inhibitors of PKA and CaMKII signaling and the chromatin immunoprecipitation (ChIP) assay, we further found that the social learning of new feeding habits in mandarin fish could be attributed to the activation of the CaMKII signaling pathway and then the stimulation of the expression of the c-fos gene, which might be an important transcriptional factor to inhibit the expression of the anorexigenic gene pomc, resulting in the food intake of dead prey fish in mandarin fish. Altogether, our results support the hypothesis that social learning could facilitate the acquisition of novel feeding habits in fish, and it considerably increases the rate of subsequent individual food intake and domestication through the interaction between the learning gene c-fos and the appetite control gene pomc.

scholarly journals Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1003
Author(s):  
Xia Luo ◽  
Yinjie Niu ◽  
Xiaozhe Fu ◽  
Qiang Lin ◽  
Hongru Liang ◽  
...  
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Brain Cell ◽  
Vaccine Production ◽  
Mandarin Fish ◽  
Siniperca Chuatsi ◽  
Stirred Bioreactor ◽  
Viral Vaccine ◽  
Chinese Perch ◽  
Large Scale Culture

Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production.

scholarly journals Transcriptome sequencing and metabolome analysis of food habits domestication from live prey fish to artificial diets in mandarin fish (Siniperca chuatsi)

2020 ◽  
Author(s):  
Shan He ◽  
Jun-Jie You ◽  
Xu-Fang Liang ◽  
Zhi-Lu Zhang ◽  
Yan-Peng Zhang
Keyword(s):  
Dna Methylation ◽  
Molecular Mechanism ◽  
Food Habits ◽  
Artificial Diets ◽  
Prey Fish ◽  
Mandarin Fish ◽  
Metabolome Analysis ◽  
Live Prey ◽  
Siniperca Chuatsi ◽  
Retinol Metabolism

Abstract Background As economical traits, food habits domestication can reduce production cost in aquaculture. However, the molecular mechanism underlying food habits domestication has remained elusive. Mandarin fish (Siniperca chuatsi) only feed on live prey fish and refuse artificial diets. In the present study, we domesticated mandarin fish to feed on artificial diets. The two groups were obtained, the fish did not eat artificial diets or ate artificial diets during all of the three domestication processes, named Group W or X, respectively. Results Using transcriptome and metabolome analysis, we investigated the differentially expressed genes and metabolites between the two groups, and found three common pathways related to food habit domestication, including retinol metabolism, glycerolipid metabolism, and biosynthesis of unsaturated fatty acids pathways. Furthermore, the western blotting and bisulfite sequencing PCR analysis were performed. The gene expression of TFIIF and histone methyltransferases ezh1 were significantly increased and decreased in the fish of Group X, respectively. The total DNA methylation levels of TFIIF gene and tri-methylation of histone H3 at lysine 27 (H3K27me3) were significantly higher and lower in the fish of Group X, respectively. Conclusion It was speculated that mandarin fish which could feed on artificial diets, might be attributed to the lower expression of ezh1, resulting in the decreased level of H3K27me3 and increased level of DNA methylation of TFIIF gene. The high expression of TFIIF gene might up-regulate the expression of genes in retinol metabolism, glycerolipid metabolism and glycerophosphoric metabolism pathways. Our study indicated the relationship between the methylation of DNA and histone and food habits domestication, which might be a novel molecular mechanism of food habits domestication in animals.

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