High molecular weight neoglycoconjugates for solid phase assays

2005 ◽  
Vol 22 (1-2) ◽  
pp. 43-51 ◽  
Author(s):  
Nadezhda V. Shilova ◽  
Oxana E. Galanina ◽  
Tatyana V. Pochechueva ◽  
Alexander A. Chinarev ◽  
Vasily A. Kadykov ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Solid Phase

An Immunoprecipitation Assay for High Molecular Weight Alkaline Phosphatase in Human Serum

1989 ◽  
Vol 26 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Gerald A Maguire ◽  
Halima Adnan
Keyword(s):  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Liver Disease ◽  
High Molecular Weight ◽  
Solid Phase ◽  
Bone Alkaline Phosphatase ◽  
Intestinal Alkaline Phosphatase ◽  
Alkaline Phosphatases ◽  
Molecular Weight Form ◽  
Hepatic Malignancies

The serum of patients with obstructive liver disease may contain a high molecular weight form of alkaline phosphatase (high Mr alkaline phosphatase). The presence of this form of alkaline phosphatase is associated with hepatic malignancies. We have investigated the use of anti-alkaline phosphatase monoclonal antibodies which do not bind high Mr alkaline phosphatase in assays for high Mr alkaline phosphatase. Direct immunoprecipitation of liver and bone alkaline phosphatase with solid phase anti-liver alkaline phosphatase antibody (which also reacts with bone alkaline phosphatase) and measurement of the residual supernatant alkaline phosphatase activity led to a precise assay. Intestinal alkaline phosphatase interfered in this assay which, consequently, was of little use in the differential diagnosis of liver disease. Indirect precipitation of liver, bone, placental and intestinal alkaline phosphatase by soluble anti-liver alkaline phosphatase (which reacts with liver and bone alkaline phosphatases), soluble anti-intestinal alkaline phosphatase (which reacts with placental and intestinal alkaline phosphatases) and solid phase anti-mouse IgG led to an assay which, although less precise, showed more promise of being useful clinically.

Solid-phase polycondensation for producing polyethylene terephthalate with a high molecular weight

1974 ◽  
Vol 5 (5) ◽  
pp. 493-495
Author(s):  
M. I. Simonova ◽  
E. M. Aizenshtein ◽  
V. V. Shevchenko
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Polyethylene Terephthalate ◽  
Solid Phase

Properties of high molecular weight polycarbonate synthesized by using the solid phase polymerization method.

2000 ◽  
Vol 57 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Hiroshi HACHIYA ◽  
Haruyuki YONEDA ◽  
Yuko MATSUDA ◽  
Kazuyuki YOSHIDA ◽  
Kunihiko TAKEDA
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Solid Phase ◽  
Polymerization Method

scholarly journals High molecular weight humic-like substances in carboneous aerosol of Ulaanbaatar city

2018 ◽  
Vol 19 (45) ◽  
pp. 5-11 ◽  
Author(s):  
Shurkhuu Tserenpil ◽  
Xing Jung Fan ◽  
Atindra Sapkota ◽  
Enkhmaa Chinzorig ◽  
Jian Zhong Song ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Carbon Content ◽  
High Molecular Weight ◽  
Solid Phase ◽  
Extraction Methods ◽  
Water Soluble ◽  
Total Carbon ◽  
Total Carbon Content ◽  
Different Seasons ◽  
Soluble Organic Fraction

Total carbon content of the atmospheric suspended particulate matters consisted of as high as 89-93% organic carbon (OC) in Ulaanbaatar aerosol without showing seasonal variation. However, limited aerosol measurements have been conducted on these OC rich aerosols particularly for high molecular weight constituents. In order to address the gap above, abundance of high molecular weight humic-like substances (HULIS) in total suspended particulates (TSP) from Ulaanbaatar atmospheric aerosol were determined for the first time. HULIS molecular structure was characterised for different seasons using carbon content and UV absorbance measurements coupled with solid phase extraction methods. Although, HULIS contributions to water soluble organic fraction of the winter and summer aerosols were similar HULIS carbon concentration was higher in winter samples (9-37 mg·L-1) than in summer (2-6 mg·L-1). Consequently quantity of aromatic moieties and degree of aromaticity varied between seasons.

Gel Entrapment of Antibody: A New Strategy for Facilitating Both Manual and Automated Radioimmunoassay

1973 ◽  
Vol 19 (12) ◽  
pp. 1339-1344 ◽  
Author(s):  
Stuart J Updike ◽  
John D Simmons ◽  
Douglas H Grant ◽  
Judith A Magnuson ◽  
Theodore L Goodfriend
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Room Temperature ◽  
Solid Phase ◽  
Binding Activity ◽  
Angiotensin I ◽  
Chromatographic Columns ◽  
New Strategy ◽  
Sample Application ◽  
Solid Phase Binding

Abstract A technique of radioimmunoassay is presented that eliminates pipetting and centrifugation, and excludes interferences by high-molecular-weight materials from the incubation and separation steps. A solid-phase binding reagent is prepared by first entrapping antibody in polyacrylamide gel. This gel is then fragmented, sieved, dried with ethanol or lyophilized, and placed in miniature disposable chromatographic columns. Application of the sample to the intra-gel column compartment is determined by the water regain of the gel. This pipetless method of sample application depends on reproducible aliquots of dry gel particles in every column. A method for preloading radiolabeled hormone and standard hormone into the column is also described. This technique has been successfully applied to the assay of angiotensin I and insulin. Dry antibody—gel stored at room temperature for 26 months has not shown loss of binding activity.

scholarly journals Serum platelet-reactive IgG of autoimmune thrombocytopenic purpura patients is not F(ab')2 mediated and a function of storage

Blood ◽  
1992 ◽  
Vol 80 (12) ◽  
pp. 3164-3172
Author(s):  
S Karpatkin ◽  
J Xia ◽  
J Patel ◽  
GJ Thorbecke
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Volume Fraction ◽  
Solid Phase ◽  
Void Volume ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombocytopenic Purpura ◽  
Autoimmune Thrombocytopenic Purpura ◽  
Control Subjects ◽  
Igg Binding

Serum platelet-reactive and glycoprotein (GP) IIb-GPIIIa-reactive IgG and F(ab')2 was examined in 39 patients with classic autoimmune thrombocytopenic purpura (ATP), two patients with anti-PLA1 antibody and 25 control subjects in an enzyme-linked immunosorbent assay. IgG was purified by diethyl aminoethyl chromatography and centrifuged at 100,000g before testing of the supernatant. Significant IgG binding (threefold to fourfold control IgG binding) was noted with 8 of 17 ATP patients' IgG, 2 anti-PLA1 IgGs, and 2 ATP patients with multiple platelet transfusions. However, F(ab')2 fragments of nine of nine positive ATP IgGs were nonreactive; F(ab')2 from the two anti-PLA1 and two multiply transfused ATP IgGs were as reactive as their intact IgG. Antiplatelet or anti-GPIIb-GPIIIa reactivity of ATP IgG could be adsorbed to fixed platelets or solid-phase GPIIb-GPIIIa and eluted with 0.1 mol/L glycine, pH 2.5. However, binding of IgG to GPIIb-GPIIIa could not be inhibited with F(ab')2 of ATP IgG or Fc fragments of control subjects. When platelet- or GPIIb-GPIIIa-reactive ATP IgG was applied to a Sephacryl 300 gel filtration column, no reactivity was noted in the 7S region, whereas anti-PLA1 localized to this region. Antiplatelet or anti-GPIIb-GPIIIa reactivity was noted in the void volume and accompanied by a high molecular weight protein region. An immunoblot of the void volume fraction with goat antihuman IgG (gamma chain) antibody showed high molecular weight bands greater than 250 Kd, which after reduction converted to a 55-Kd heavy-chain band. Fresh samples of ATP and control IgG processed within 1 to 2 days of blood withdrawal had no reactivity for GPIIb-GPIIIa. After storage at -20 degrees C for greater than 3 months, 5 of 19 ATP IgG became reactive, whereas 16 of 16 controls were nonreactive. Thus, platelet-reactive IgG of ATP sera appears to be caused by the development of IgG aggregates held together by disulfide bonds that develop on storage, and is not F(ab')2 mediated.

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