Arbuscular mycorrhiza improved growth performance in Macadamia tetraphylla L. grown under water deficit stress involves soluble sugar and proline accumulation

2012 ◽  
Vol 69 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Suravoot Yooyongwech ◽  
Nuttawuth Phaukinsang ◽  
Suriyan Cha-um ◽  
Kanyaratt Supaibulwatana
Keyword(s):  
Growth Performance ◽  
Arbuscular Mycorrhiza ◽  
Water Deficit ◽  
Soluble Sugar ◽  
Proline Accumulation ◽  
Water Deficit Stress

Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress

2016 ◽  
Vol 43 (10) ◽  
pp. 939 ◽  
Author(s):  
Asma Jday ◽  
Kilani Ben Rejeb ◽  
Ines Slama ◽  
Kaouthar Saadallah ◽  
Marianne Bordenave ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Water Deficit ◽  
Stress Responses ◽  
Lipid Membrane ◽  
Proline Accumulation ◽  
Shoot Biomass ◽  
Water Deficit Stress ◽  
Leaf Water Content ◽  
Protein Levels ◽  
Cakile Maritima

Nitric oxide (NO) – an endogenous signalling molecule in plants and animals – mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima Scop. seedlings under water deficit stress using sodium nitroprusside (SNP) as NO donor and as a pre-treatment before the application of stress. Water deficit stress was applied by withholding water for 14 days. Growth, leaf water content (LWC), osmotic potential (ψs), chlorophyll, malondialdehyde (MDA), electrolyte leakage (EL), proline and Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) protein levels were determined. Enzyme activities involved in antioxidant activities (superoxide dismutase (SOD) and catalase (CAT)) were measured upon withholding water. The results showed that shoot biomass production was significantly decreased in plants subjected to water deficit stress alone. However, in water deficit stressed plants pre-treated with SNP, growth activity was improved and proline accumulation was significantly increased. Proline accumulation was concomitant with the stimulation of its biosynthesis as shown by the accumulation of P5CS proteins. Nevertheless, no significant change in ProDH protein levels was observed. Besides plants showed lower water deficit-induced lipid membrane degradation and oxidative stress after the pretreatment with 100 µM SNP. This behaviour was related to the increased activity of SOD and CAT. Thus, we concluded that NO increased C. maritima drought tolerance and mitigated damage associated with water deficit stress by the regulation of proline metabolism and the reduction of oxidative damage.

scholarly journals Physiological and Antioxidant Parameters in Two Lycoris Species as Influenced by Water Deficit Stress

HortScience ◽  
2015 ◽  
Vol 50 (11) ◽  
pp. 1702-1708 ◽  
Author(s):  
Sheng Xu ◽  
Mingmin Jiang ◽  
Jiangyan Fu ◽  
Lijian Liang ◽  
Bing Xia ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Water Deficit ◽  
Enzyme Activities ◽  
Water Status ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Net Photosynthesis ◽  
Water Deficit Stress ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase

From a field experiment, the changes in morphophysiological characters and antioxidant enzyme activities were studied in two Lycoris species (Lycoris radiata and Lycoris aurea) subjected to 16 days of water deficit stress. With the increase of water deficit stress processing time, leaf relative water content (RWC), membrane stability index (MSI), net photosynthesis (Pn), stomatal conductance (gS), transpiration rate (E), and chlorophyll (Chl) content decreased in both studied species. The water use efficiency (WUE) showed an opposite tendency between the two species under water deficit stress, where WUE of L. aurea decreased moderately and WUE of L. aurea increased somehow. Intercellular CO2 concentration (Ci) in L. aurea and L. radiata decreased in respond to water deficit stress at early stages of stress treatment, then increased throughout the rest of the stress period, and reached levels higher than those in well-watered plants at the end of the treatment. In addition, there was a significant increment in soluble sugar content and proline accumulation under water deficit stress in both species, and L. radiata showed a much more accumulation. The activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) increased in both plants subjected to water deficit stress while declined as the stress time increased. In L. aurea, catalase (CAT) showed a sustained increment, but it responded later and after a transient increase declined again in L. radiata under water deficit stress. In conclusion, L. radiata was more tolerant to water deficit stress than L. aurea as evidenced by its relatively higher water status, higher levels of proline, soluble sugar and pigments, and stronger photoprotection. Moreover, relatively higher antioxidant enzyme activities and lower levels of thiobarbituric acid reactive substances (TBARS) in L. radiata were also associated with its better protection against water deficit stress-induced oxidative damage.

scholarly journals Biochemical Response and Nutrient Uptake of Two Arbuscular Mycorrhiza-Inoculated Chamomile Varieties Under Different Water Potentials

2021 ◽  
Author(s):  
Fatemeh Ebrahimi ◽  
Amin Salehi ◽  
Mohsen Movahedi Dehnavi ◽  
Amin Mirshekari ◽  
Mohammad Hamidian ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Arbuscular Mycorrhiza ◽  
Water Deficit ◽  
Crop Production ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Water Deficit Stress ◽  
Water Potentials ◽  
Randomized Design ◽  
Total Dry Weight

Abstract BackgroundWater-deficit stress is one of the most important sources of damage to crop production worldwide. Adopting appropriate varieties using soil microorganisms such as arbuscular mycorrhiza(AM) can significantly reduce theadverseeffectsofwater deficiency.This study is aimed to evaluate the role of Funneliformismosseaeon nutrients uptake and some physiological traits of two chamomile varieties namely Bodgold (Bod) and Soroksári(Sor) under water-deficit stress. The pot experiment was performed in a hydroponic system within a completely randomized design considering four replications. Three levels of water-deficit stress (PEG 6000) were taken into account at water potentials of -0.4 and -0.8MPa. The second factor was AM inoculation.ResultsWater-deficit stress significantly reduced the uptake of macro-nutrients (N, P, and K) and micro-nutrients (Fe, Cu, Mn, and Zn) in the shoots and roots. Moreover, the level of osmolytes (total soluble sugars and proline) and the activity of antioxidant enzymes in the shoots of both varieties increased under water-deficit stress. In the case of Sor variety, the level of these compounds was more satisfactory. AM improved plant nutrition uptake and osmolyte contents while enhancing antioxidant enzymes and reducing theadverseeffectsofwater-deficit stress. Under water-deficit stress, the growth and total dry weight improved upon AM inoculation. ConclusionsIn general, inoculation of chamomile with AM balanced the uptake of nutrients increased the level of osmolytes, antioxidant enzymes, and hence improved plant characteristics under water-deficit stress in both varieties, however, it was more effective in reducing stress damages in Sor variety.

scholarly journals Biochemical Response and Nutrient Uptake of Two Arbuscular Mycorrhiza-Inoculated Chamomile Varieties under Different Water Potentials

2021 ◽  
Author(s):  
Fatemeh Ebrahimi ◽  
Amin Salehi ◽  
Mohsen Movahedi Dehnavi ◽  
Amin Mirshekari ◽  
Mohammad Hamidian ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Arbuscular Mycorrhiza ◽  
Water Deficit ◽  
Crop Production ◽  
Soluble Sugars ◽  
Am Fungi ◽  
Dry Weight ◽  
Water Deficit Stress ◽  
Randomized Design ◽  
Total Dry Weight

Abstract BackgroundWater-deficit stress is one of the most important sources of damage to crop production worldwide. Adopting appropriate varieties using soil microorganisms such as arbuscular mycorrhiza(AM) fungi can significantly reduce the adverse effects of water deficiency. This study is aimed to evaluate the role of Funneliformis mosseae on nutrients uptake and some physiological traits of two chamomile varieties namely Bodgold (Bod) and Soroksári (Sor) under water-deficit stress. The pot experiment was performed in a completely randomized design with three factors: water-deficit stress (PEG 6000) was applied along with Hoagland solution at three levels (0, -0.4 and -0.8 MPa), two German chamomile varieties (Bodgold (Bod) and Soroksari (Sor)) and AM inoculation (Funneliformis mosseae species (fungal and non-fungal)) at four replications in perlite substrate. ResultsWater-deficit stress significantly reduced the uptake of macro-nutrients (N, P, and K) and micro-nutrients (Fe, Cu, Mn, and Zn) in the shoots and roots. Moreover, the level of osmolytes (total soluble sugars and proline) and the activity of antioxidant enzymes in the shoots of both varieties increased under water-deficit stress. In the case of Sor variety, the level of these compounds was more satisfactory. AM improved plant nutrition uptake and osmolyte contents while enhancing antioxidant enzymes and reducing the adverse effects of water-deficit stress. Under water-deficit stress, the growth and total dry weight improved upon AM inoculation. ConclusionsIn general, inoculation of chamomile with AM balanced the uptake of nutrients increased the level of osmolytes, antioxidant enzymes, and hence improved plant characteristics under water-deficit stress in both varieties, however, it was more effective in reducing stress damages in Sor variety.

scholarly journals Evaluation of the Morpho-Physiological, Biochemical and Molecular Responses of Contrasting Medicago truncatula Lines under Water Deficit Stress

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2114
Author(s):  
Loua Haddoudi ◽  
Sabrine Hdira ◽  
Mohsen Hanana ◽  
Irene Romero ◽  
Imen Haddoudi ◽  
...  
Keyword(s):  
Water Deficit ◽  
Medicago Truncatula ◽  
Soluble Sugar ◽  
Membrane Integrity ◽  
Growth And Yield ◽  
Water Deficit Stress ◽  
Molecular Responses ◽  
Forage Crop ◽  
Cell Membrane Integrity ◽  
Tolerant Line

Medicago truncatula is a forage crop of choice for farmers, and it is a model species for molecular research. The growth and development and subsequent yields are limited by water availability mainly in arid and semi-arid regions. Our study aims to evaluate the morpho-physiological, biochemical and molecular responses to water deficit stress in four lines (TN6.18, JA17, TN1.11 and A10) of M. truncatula. The results showed that the treatment factor explained the majority of the variation for the measured traits. It appeared that the line A10 was the most sensitive and therefore adversely affected by water deficit stress, which reduced its growth and yield parameters, whereas the tolerant line TN6.18 exhibited the highest root biomass production, a significantly higher increase in its total protein and soluble sugar contents, and lower levels of lipid peroxidation with greater cell membrane integrity. The expression analysis of the DREB1B gene using RT-qPCR revealed a tissue-differential expression in the four lines under osmotic stress, with a higher induction rate in roots of TN6.18 and JA17 than in A10 roots, suggesting a key role for DREB1B in water deficit tolerance in M. truncatula.

scholarly journals Physiological Evaluation of Wheat Genotypes for Tolerance to Water Deficit Stress

2018 ◽  
Vol 20 (2) ◽  
pp. 37-52
Author(s):  
MS Rana ◽  
MA Hasan ◽  
MM Bahadur ◽  
MR Islam
Keyword(s):  
Grain Yield ◽  
Water Deficit ◽  
Flag Leaf ◽  
Soluble Sugar ◽  
Water Deficit Stress ◽  
Dry Matter Accumulation ◽  
Growth Duration ◽  
Wheat Genotypes ◽  
Leaf Chlorophyll ◽  
Biological Yield

Four wheat genotypes (BARI Gom 25, E 28, BAW 1170, BAW1140) were grown under well water and water deficit stress condition to evaluate the sensitivity of physiological traits of wheat resulting reduced grain yield under water deficit stress. Wheat genotypes showed greater stability of flag leaf chlorophyll, greater ability to retain water in leaf, higher level of proline in flag leaf and kernel, higher level of soluble sugar in flag leaf and greater ability to keep the canopy cooler compared to sensitive genotype under water deficit condition. Greater spike dry matter accumulation at peak, longer grain growth duration, better yield components such as spikes m-2 and grains spike-1 under water deficit stress contributed to better tolerance of BARI Gom 25, E 28 and BAW 1170. The order of tolerance based on grain yield was BAW 1170 > BARI Gom 25 > E 28 > BAW 1140 and the order of tolerance based on above ground biological yield was BAW 1170 > E 28 > BARI Gom 25 > BAW 1140.Bangladesh Agron. J. 2017, 20(2): 37-52

scholarly journals Abscisic acid-induced nitric oxide and proline accumulation in independent pathways under water-deficit stress during seedling establishment in Medicago truncatula

2014 ◽  
Vol 65 (8) ◽  
pp. 2161-2170 ◽  
Author(s):  
Elisabeth Planchet ◽  
Isabelle Verdu ◽  
Julien Delahaie ◽  
Caroline Cukier ◽  
Clément Girard ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Abscisic Acid ◽  
Water Deficit ◽  
Medicago Truncatula ◽  
Seedling Establishment ◽  
Proline Accumulation ◽  
Water Deficit Stress

Differential performance of two forage species, Medicago truncatula and Sulla carnosa, under water-deficit stress and recovery

2013 ◽  
Vol 64 (3) ◽  
pp. 254 ◽  
Author(s):  
Aida Rouached ◽  
Inès Slama ◽  
Walid Zorrig ◽  
Asma Jdey ◽  
Caroline Cukier ◽  
...  
Keyword(s):  
Water Deficit ◽  
Medicago Truncatula ◽  
Osmotic Potential ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Membrane Integrity ◽  
Shoot Biomass ◽  
Water Deficit Stress ◽  
Leaf Osmotic Potential ◽  
Forage Species

The response patterns during water deficit stress and subsequent recovery of two forage species, Medicago truncatula and Sulla carnosa, were studied. After germination and pre-treatment, seedlings were individually cultivated for two months under two irrigation modes: 100% and 33% of field capacity. Measured parameters were plant growth, water relations, leaf osmotic potential, lipid peroxidation, and leaf inorganic (Na+ and K+) and organic (proline and soluble sugars) solute contents, as well as delta-1-pyrroline-5-carboxylate synthase (P5CS) and proline dehydrogenase (PDH) activities. Our results showed that under control conditions, and in contrast to roots, no significant differences were observed in shoot biomass production between the two species. However, when subjected to water-deficit stress, M. truncatula appeared to be more tolerant than S. carnosa (reduction by 50 and 70%, respectively). In the two studied species, water-deficit stress led to an increase in root/shoot ratio and leaf proline and soluble sugar contents, and a decrease in leaf osmotic potential. Enzymatic assay revealed that in the two species, P5CS activity was stimulated whereas that of PDH was inhibited under stress conditions. Despite greater accumulation of proline, sugar, and potassium in leaves of S. carnosa, M. truncatula was more tolerant to water deficit. This was essentially due to its capacity to control tissue hydration and water-use efficiency, in addition to its greater ability to protect membrane integrity. Following stress relief, M. truncatula and S. carnosa showed partial re-establishment of growth capacity.

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