Effect of Impurities on the Freezing Point of Zinc

2017 ◽  
Vol 38 (3) ◽  
Author(s):  
Jianping Sun ◽  
Steffen Rudtsch ◽  
Yalu Niu ◽  
Lin Zhang ◽  
Wei Wang ◽  
...  

The technique is described of achieving the highest accuracy of measurement with platinum-resistance thermometers at the freezing point of zinc and the boiling point of sulphur. The two points are compared in a series of measurements and it is found that the zinc point is some three or four times more reproducible than the sulphur point. It is concluded that the substitution of the zinc point for the sulphur point as a primary fixed point of the International Temperature Scale would lead to a greater precision in the definition of the scale. The value of the freezing point of zinc is found to be 419∙5055 ± 0∙002°C.


2014 ◽  
Vol 35 (6-7) ◽  
pp. 1134-1146 ◽  
Author(s):  
Jianping Sun ◽  
Steffen Rudtsch ◽  
Jintao Zhang ◽  
Xincai Wu ◽  
Xiaolong Deng ◽  
...  

Author(s):  
E.T. O’Toole ◽  
G.P. Wray ◽  
J.R. Kremer ◽  
J.R. Mcintosh

Ultrarapid freezing and cryomicroscopy of frozen hydrated material makes it possible to visualize samples that have never been exposed to chemical fixatives, dehydration, or stains. In principle, freezing and cryoimaging methods avoid artifacts associated with chemical fixation and processing and allow one to visualize the specimen in a condition that is close to its native state. Here we describe a way to use a high voltage electron microscope (HVEM) for the cryoimaging of frozen hydrated PTK1 cells.PTK1 cells were cultured on formvar-coated, carbon stabilized gold grids. After three days in culture, the grids were removed from the culture medium and blotted in a humidity chamber at 35° C. In some instances, the grids were rinsed briefly in 0.16 M ammonium acetate buffer (pH 7.2) prior to blotting. After blotting, the grids were transferred to a plunging apparatus and plunged into liquid ethane held directly above its freezing point. The plunging apparatus consists of a vertical slide rail that guides the fall of a mounted pair of forceps that clamp the specimen. The forceps are surrounded by a plexiglass humidity chamber mounted over a dewar of liquid nitrogen containing an ethane chamber. After freezing, the samples were transferred to liquid nitrogen and viewed in a JEOL JEM 1000 equipped with a top entry cold stage designed and built by Mr. George Wray (Univ. Colorado). The samples were routinely exposed to electron doses of 1 e/Å2/sec, and viewed at a temperature of −150° C. A GATAN video system was used to enhance contrast and to estimate the correct amount of underfocus needed to obtain phase contrast at various magnifications. Low dose micrographs were taken using two second exposures of Kodak 4463 film. The state of the solid water in the specimen was determined by diffraction using a 30/μm field limiting aperture and a camera length of 1 meter.


Author(s):  
A. Vovkohon ◽  
V. Nadtochiy ◽  
G. Kalinina ◽  
O. Hrebelnyk ◽  
N. Fedoruk ◽  
...  

The article highlights comparative research results of milk quality indices obtained from the milking in specialized milking halls with such milking units as «Parallel», «Carousel» or in stalls with the milking unit «Molokoprovid». The fat and protein mass fraction, dry matter and fat-free dry matter, density, titratable and active acidity, heat resistance and freezing point have been determined according to the accepted techniques. The electrical conductivity of milk has been determined by using the analytical device MD-20 MAS-D-TEC. The total amount of milk bacteria has been determined by reductase reduction test and by seeding method in Petri dish. The milk quality has been investigated by the fermentation and rennet fermentation tests. The higher indices of the fat mass fraction, the protein mass fraction and the dry substance concentration of milk, obtained in specialized milking halls, have been established. This is not statistically significant. Milk, obtained from the milking unit «Molokoprovid», has higher index of titratable acidity, lower thermal stability in comparison with milk, obtained from specialized milking halls with milking units «Parallel» and «Carousel». It has been determined that there is the bacteria insemination increase in milk received from milking cows in stalls in comparison with milk, obtained from milking in specialized halls. Milk, obtained from the milking unit «Carousel», indicates the subclinical form of mastitis in cows or «Carousel» operation violationif there is in 1,8 mS/cm conductivity increase above average index 4,6 mS/cm. Key words: technology, quality and safety of milk, milking, milking unit, milking hall, bacterial insemination.


2019 ◽  
Vol 7 (1) ◽  
pp. 12-22
Author(s):  
Ratu Mutia Fajarani ◽  
Yopi Handoyo ◽  
Raden Hengki Rahmanto

Cooling is the best preservation method than others because the food that has been cooled will remain fresh and will not experience a change in taste, color and aroma, besides all the activities that cause decay will stop so that the cooled food will last longer. (Hartanto, 1984). With the proper cooling engine planning, it can help with spatial adjustments, adjustments to loading, estimation of the power to be used, and budget plans. That is what is commonly called the cooling load calculation. Calculation of cooling load needs to be carried out before planning. This is necessary because the magnitude of the pending load is very influential on the selection of the cooling engine so that the freezing point for preserving food can be accurate. Pendiginan burden is influenced by external and internal factors. With the experimental method, it is obtained the results of the external cooling load as the external cooling load is 11.6 kW, the inner cooling load is 138.8 kW and the performance work coefficient (COP) is 2.


2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.


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