scholarly journals Anti-inflammatory activity of palmitoylethanolamide ameliorates osteoarthritis induced by monosodium iodoacetate in Sprague–Dawley rats

2021 ◽  
Vol 29 (5) ◽  
pp. 1475-1486
Author(s):  
Jae In Jung ◽  
Hyun Sook Lee ◽  
Young Eun Jeon ◽  
So Mi Kim ◽  
Su Hee Hong ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Acid Amide ◽  
Treatment Strategies ◽  
Leukotriene B4 ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Monosodium Iodoacetate ◽  
Anti Inflammatory

AbstractNovel treatment strategies are urgently required for osteoarthritis (OA). Palmitoylethanolamide (PEA) is a naturally occurring fatty acid amide with analgesic and anti-inflammatory effects. We aimed to examine its effect on OA and elucidate the molecular mechanism of actions in monosodium iodoacetate (MIA)-induced OA Sprague–Dawley rats. The experimental animals were divided into normal control group (injected with saline + treated with phosphate-buffered saline (PBS), NOR), control group (injected with MIA + treated with PBS, CON), 50 or 100 mg/kg body weight (BW)/day PEA-treated group (injected with MIA + treated with 50 or 100 mg of PEA/kg BW/day, PEA50 or PEA100), and positive control group (injected with MIA + treated with 6 mg of diclofenac/kg BW/day, DiC). The changes in blood parameters, body parameters, gene expression of inflammatory mediators and cytokines, knee thickness, and joint tissue were observed. Oral administration of PEA had no adverse effects on the BW, liver, or kidneys. PEA reduced knee joint swelling and cartilage degradation in MIA-induced OA rats. The serum levels of leukotriene B4, nitric oxide, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and prostaglandin E2 considerably reduced in the PEA100 group compared with those in the CON group. In the synovia of knee joints, the mRNA expression of iNOS, 5-Lox, Cox-2, Il-1β, Tnf-α, and Mmp-2, -3, -9, and -13 apparently increased with MIA administration. Meanwhile, Timp-1 mRNA expression apparently decreased in the CON group but increased to the normal level with PEA treatment. Thus, PEA can be an effective therapeutic agent for OA.

scholarly journals The Anti-Inflammatory Activity of Palmitoylethanolamide Ameliorates Osteoarthritis Induced by Monosodium Iodoacetate in Sprague Dawley Rats

2021 ◽  
Author(s):  
Jae In Jung ◽  
Hyun Sook Lee ◽  
Young Eun Jeon ◽  
So Mi Kim ◽  
Su Hee Hong ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Acid Amide ◽  
Leukotriene B4 ◽  
Joint Disease ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Monosodium Iodoacetate ◽  
Anti Inflammatory

Abstract Novel treatment strategies are urgently required for osteoarthritis (OA), a degenerative joint disease. Palmitoylethanolamide (PEA) is a naturally occurring fatty acid amide with analgesic and anti-inflammatory effects. We aimed to examine its effect on OA and molecular mechanism of actions in monosodium iodoacetate (MIA)-induced OA Sprague Dawley rats. The experimental animals were divided into five groups: normal control group (injected with saline + treated with phosphate-buffered saline (PBS), NOR), control group (injected with MIA + treated with PBS, CON), 50 or 100 mg/kg body weight (BW)/day PEA-treated group (injected with MIA + treated with 50 or 100 mg of PEA/kg BW/day, PEA50 or PEA 100), and positive control group (injected with MIA + treated with 6 mg of diclofenac/kg BW/day, DiC). Changes in blood and body parameters, gene expression of inflammatory mediators and cytokines, knee thickness, and joint tissue were observed. We found no adverse effects of oral administration of PEA on BW, liver, or kidneys. PEA reduced knee joint swelling and cartilage degradation in MIA-induced OA rats. The serum levels of leukotriene B4, nitric oxide, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and prostaglandin E2 considerably reduced in the PEA100 group compared to those in the CON group. In the synovia of knee joints, mRNA expression of iNOS, 5-Lox, Cox-2, Il-1β, Tnf-α, Mmp-2, -3, -9, and − 13 noticeably reduced with MIA administration. Meanwhile, Timp-1 mRNA expression noticeably decreased in the CON group but increased to the normal level with PEA treatment. Thus, we demonstrated that PEA can be used as an effective therapeutic agent for OA.

scholarly journals Anti-inflammatory activity of aqueous extracts of Ove Costa Rican medicinalplants in Sprague-Dawley rats

2015 ◽  
pp. 723-727
Author(s):  
Beatriz Badilla ◽  
Gerardo Mora ◽  
Luis Jorge Poveda
Keyword(s):  
Costa Rican ◽  
Inflammatory Activity ◽  
Control Group ◽  
Aqueous Extracts ◽  
Paw Edema ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Lethal Doses

The anti-inflamrnatory properties of Loasa specic¡sa and Loasa triphylla (Loasaceae), Urtica leptuphylla and Urera baccifera (Urticaceae), and Chaptalia nutans (Astemceae) were studied using the carregeenan induced mt paw edema model. Aqueous extmcts of each plant were made according to the ethnoboranical use. The hippocratic assay was made with female mts; the dose used was 500 mglkg i.p. andthe control group received 0.5 mi of n.s.s .. A11 the lmimals treated showed hipothermia, and those treated with the extmcts of Chaptalia nutans, Urera baccifera and Urtica lept/lPhylla showed an increased colinergic activity. Acute toxicities of !he aqueous extmcts were studied in mice an tbe mean lethal doses ranged between 1.0226 and 1.2022 glkg. The extracts of Urera baccifera, Chaptalia nutans, Loasa speciosa and Loasa triphylla (500 mglkg i.p.) showed an antiinflarnmatory activity compamble with that of indomethacin. The extracts of U. baccifera and C. nutans, which showed the gI"tatest anti-inflamrtJatory activity, did not show it when used orally (500 mg/k:g p.o.).

scholarly journals Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 623
Author(s):  
Liza Meutia Sari ◽  
Zaki Mubarak ◽  
Dina Keumala Sari
Keyword(s):  
Triamcinolone Acetonide ◽  
Positive Control ◽  
Flowering Plant ◽  
Positive Control Group ◽  
Control Group ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sprague Dawley Rats ◽  
Tnf Α

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into five groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), and F3 (treatment with 80% areca nut:20% chrysanthemum). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2 and positive control group (Dependent t-test, p<0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p<0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test). Similar results were seen in the collagenases assay. Conclusions: A balanced combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in rats through the increase of TNF-α and collagen deposition.

Gold Nanoparticles with Dexmedetomidine Regulate GSK-3β to Reduce Neurocognitive Effects in Anesthetized Rats

2021 ◽  
Vol 21 (12) ◽  
pp. 6205-6211
Author(s):  
Xiaoxia Zhang ◽  
Zumin Xing ◽  
Jiyuan Li ◽  
Shuyi Tang ◽  
Yiwen Zhang
Keyword(s):  
Gold Nanoparticles ◽  
Mrna Expression ◽  
Intraperitoneal Injection ◽  
Control Group ◽  
Sprague Dawley ◽  
Rt Pcr ◽  
Anesthetized Rats ◽  
Sprague Dawley Rats ◽  
Gsk 3Β ◽  
Neurocognitive Effect

The aim of this study was to explore the neurocognitive effects of dexmedetomidine-loaded gold nanoparticles (AuNPs-dexmedetomidine) on anesthetized rats. Sixty Sprague Dawley rats (age, 2–3 weeks; weight, 250–280 g) were randomly divided into three groups (n = 20): the control group and two groups that received intraperitoneal injection of AuNPs-dexmedetomidine at 50 and 100 μg/kg each. Western blotting and RT-PCR were used to determine the protein and mRNA expression of GSK-3β, respectively. Compared with that in the control group, GSK-3β expression in AuNP-dexmedetomidine groups increased (P < 0.05). The protein expression of GSK-3β was higher and mRNA expression was significantly lower in the 100 μg/kg AuNP-dexmedetomidine group (P < 0.05). AuNPs-dexmedetomidine reduced the neurocognitive effect on anesthetized rats through the regulation of the GSK-3β signaling pathway.

scholarly journals Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 623
Author(s):  
Liza Meutia Sari ◽  
Zaki Mubarak ◽  
Dina Keumala Sari
Keyword(s):  
Triamcinolone Acetonide ◽  
Positive Control ◽  
Flowering Plant ◽  
Positive Control Group ◽  
Control Group ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sprague Dawley Rats ◽  
Tnf Α

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into five groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), and F3 (treatment with 80% areca nut:20% chrysanthemum). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2, F3, and positive control group (Dependent t-test, p<0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p<0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test, p<0.05). In the collagenases assay, a high concentration of areca nut (F3) induced the abundance of collagen during the ulcer healing process. Conclusions: The combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in Sprague-Dawley rats through the increase of TNF-α and collagen deposition.

scholarly journals The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

2019 ◽  
Author(s):  
Jie Guo ◽  
Xiaolu Cao ◽  
Xianmin Hu ◽  
Shulan Li ◽  
Jun Wang
Keyword(s):  
Oxidative Stress ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Tunel Staining ◽  
High Dose ◽  
Sprague Dawley ◽  
Curcumin Treatment ◽  
Concurrent Administration ◽  
Tnf Α ◽  
Anti Inflammatory

Abstract Background: As a chemical extensively used in industrial areas as well as formed during heating of carbohydrate-rich food and tobacco, acrylamide (ACR) has been known as well-established neurotoxic pollutant. Although the precise mechanism is unclear, enhanced apoptosis, oxidative stress and inflammation have been demonstrated to contribute to the ACR-induced neurotoxicity. In this study, we assessed the possible anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin, the most active component in a popular spice known as turmeric, on the neurotoxicity caused by ACR in rats. Methods: Curcumin at the dose of 50 and 100 mg/kg was orally given to ACR- intoxicated Sprague-Dawley rats exposed by ACR at 40mg/kg for 4 weeks. All rats were subjected to behavioral analysis. The HE staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) staining were used to detect histopathological changes and apoptotic cells, respectively. The mRNA and protein expressions of apoptosis-related molecule telomerase reverse transcriptase (TERT) were detected using real-time PCR and immunohistochemistry, respectively. The contents of malondialdehyde (MDA) and glutathione (GSH) as well as the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured as the indicators for evaluating the level of oxidative stress in brain. The levels of pro-inflammatory cytokinestumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in cerebral homogenates were detected using ELISA assay. Results: Concurrent administration of curcumin at the oral doses of 50 and 100 mg/kg with ACR significantly protected the rats from ACR-induced weigh loss and motor function deficits, and improved the pathological alterations in the ACR-intoxicated brains. Curcumin treatment especially at a high dose enhanced the TERT mRNA expression level and increased the number of TERT-positive nerve cells in cortex tissues of ACR intoxicated rats. The levels of MDA, TNF-α and IL-1β in the cerebral homogenates were reduced, the contents of GSH as well as the activities of SOD and GSH-Px were increased by curcumin treatment, compared to ACR control group. Conclusions: These data suggested the anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on ACR-induced neurotoxicity in rats. And maintaining TERT-related anti-apoptotic function might be one mechanism underlying the protective effect of curcumin on ACR-intoxicated brains.

scholarly journals ANTI-INFLAMMATORY EFFECT OF ZINGIBER OFFICINALE ON SPRAGUE DAWLEY RATS

2017 ◽  
Vol 10 (3) ◽  
pp. 353 ◽  
Author(s):  
Rohini Karunakaran ◽  
Ndanusa Abdullahi Hassan ◽  
Uma Sankar A ◽  
Khin Mar Aye
Keyword(s):  
Aqueous Extract ◽  
Diclofenac Sodium ◽  
Zingiber Officinale ◽  
Control Group ◽  
Sprague Dawley ◽  
Reference Drug ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Anti Inflammatory ◽  
Inflammatory Effect

ABSTRACTObjective: To investigate the anti-inflammatory effect of aqueous extract of Zingiber officinale on carrageenan-induced inflammation on SpragueDawley (SD) rats.Methods: SD rats were divided into six of five groups and allowed to acclimatize for 1 week. Inflammation was induced on the animal by injecting theright hand paw with carrageenan (0.1 ml of 1%). Group 1 was given normal saline and served as a control. Group 2 was fed with food and water andserved as the carrageenan control. Group 3 was given 200 mg/kg aqueous extract of ginger, Group 4 with 400 mg/kg aqueous extract of ginger, andGroup 5 with 150 mg/kg diclofenac sodium (reference drug for inflammation).Results: The paw edema in carrageenan-induced SD rats was considerably reduced by treating with 400 mg/kg aqueous ginger extracts whencompared to the untreated SD rats (p<0.001).Conclusion: This study indicates that aqueous extract of Z. officinale possesses anti-inflammatory properties.Keywords: Anti-inflammatory, Sprague Dawley rats, Zingiber officinale, Carrageenan, Edema

scholarly journals Anti-inflammatory effect of egg white-chalcanthite and purple bamboo salts mixture on arthritis induced by monosodium iodoacetate in Sprague-Dawley rats

2016 ◽  
Vol 32 (2) ◽  
pp. 91 ◽  
Author(s):  
Tae-Hee Lee ◽  
Hyun-kyung Song ◽  
Ja-Young Jang ◽  
Dong-Yoon Kim ◽  
Hyun-Kyung Park ◽  
...  
Keyword(s):  
Egg White ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Monosodium Iodoacetate ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals THE POSSIBLE EFFECTS OF SILYMARIN ON CEREBRUM WITH EXPERIMENTAL HEPATIC ENCEPHALOPATHY IN RATS

2020 ◽  
Vol 8 (8) ◽  
pp. 140-146
Author(s):  
Ozgun Teksoy ◽  
Varol Sahinturk ◽  
Mustafa CENGİZ ◽  
Behcet İnal ◽  
Adnan Ayhancı
Keyword(s):  
Hepatic Encephalopathy ◽  
Brain Tissue ◽  
Immunohistochemical Analysis ◽  
Control Group ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Dorsolateral Prefrontal ◽  
High Doses

Background: The relationship between liver diseases and neurological defects is well established. Hepatic encephalopathy (HE) has been seen both in people with acute liver failure (ALF) and chronic liver disease (CLF). HE is a complex neuropsychiatric syndrome that is seen in patients suffering from liver dysfunction. Silymarin (Sm) has antioxidant, anti-inflammatory, and anti-carcinogenic features. In this study, the possible protective effects of silymarin were investigated against dorsolateral prefrontal cortex (DLPFC) damage induced by thioacetamide (TAA). Method: To achieve this, male Sprague Dawley rats (200-250 g) were randomly divided into four groups, with 7 animals comprising each group: the control group, 50 mg/kg TAA group, 50 mg/kg Sm + TAA group, and 100 mg / kg Sm + TAA group. Results: Differences between the groups were determined by performing immunohistochemical analysis of the PFC. Bax, TNF-α, and TUNEL expression increased in the brain tissue of the experimental group where only TAA was administered. Conclusions: It was observed that in high doses in particular (100 mg/kg Sm + TAA group), Sm was effective in preventing PFC damage caused by TAA. It was determined that 100 mg/kg Sm significantly reduces TAA-induced inflammation (TNF-α and H&E) and apoptosis (Bax, TUNEL) in brain tissue.

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