Assimilation of inorganic nitrogen for scaling up Desmodesmus communis (Scenedesmaceae) biomass production

Four microalgae species were evaluated for their bioremediation capacity of anaerobic digestion effluent (ADE) rich in ammonium nitrogen, derived from a biogas plant. Chlorella vulgaris, Chlorella sorokiniana, Desmodesmus communis and Stichococcus sp. were examined for their nutrient assimilation efficiency, biomass production and composition through their cultivation in 3.7% v/v ADE; their performance was compared with standard cultivation media which consisted in different nitrogen sources, i.e., BG-11NO3 and BG-11ΝΗ4 where N-NO3 was replaced by N-NH4. The results justified ammonium as the most preferable source of nitrogen for microalgae growth. Although Stichococcus sp. outperformed the other 3 species in N-NH4 removal efficiency both in BG-11NH4 and in 3.7% ADE (reaching up to 90.79% and 69.69% respectively), it exhibited a moderate biomass production when it was cultivated in diluted ADE corresponding to 0.59 g/L, compared to 0.89 g/L recorded by C. vulgaris and 0.7 g/L by C. sorokiniana and D. communis. Phosphorus contained in the effluent and in the control media was successfully consumed by all of the species, although its removal rate was found to be affected by the type of nitrogen source used and the particular microalgae species. The use of ADE as cultivation medium resulted in a significant increase in carbohydrates content in all investigated species.

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Novel denitrifying bacteria Pseudomonas stutzeri strain D1 - from isolation to the biomass production

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq190111018v ◽

2019 ◽

Vol 25 (4) ◽

pp. 403-415

Author(s):

Ana Vidakovic ◽

Olja Sovljanski ◽

Damjan Vucurovic ◽

Gordana Racic ◽

Milan Djilas ◽

...

Keyword(s):

Biomass Production ◽

Reference Strain ◽

Ph Value ◽

Pseudomonas Stutzeri ◽

Scaling Up ◽

Optimal Conditions ◽

Viable Cells ◽

Vitek 2 ◽

Inoculum Volume ◽

Denitrification Genes

An aerobic denitrifier was newly isolated and identified by VITEK? 2 Compact System and MALDI-TOF MS as P. stutzeri strain D1. Sequence amplification indicates that the denitrification genes napA, nirS, norB and nosZ are present in a novel strain D1, as well as in reference strain ATCC 17588. Strain D1 had capability to fully remove 3 g/L of nitrate (as KNO3) in 48 h, while the reference strain completed this task in 60 h. Single factor experiments indicate that the optimal conditions for biomass production were: temperature of 30?C, pH value of 7 and inoculum volume of 5 vol.%. Scaling up of biomass production of both denitrifiers was successfully performed in 3 and 7 L laboratory bioreactors by reaching 9 log CFU/mL of the viable cells. The results demonstrate the feasibility of using investigated P. stutzeri strains in denitrification processes and the simplicity of the up-scaling of biomass production for the treatment of large areas contaminated with nitrate.

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Recovering wasted nutrients from shrimp farming through the combined culture of polychaetes and halophytes

Scientific Reports ◽

10.1038/s41598-021-85922-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Daniel Jerónimo ◽

Ana Isabel Lillebø ◽

Javier Cremades ◽

Paulo Cartaxana ◽

Ricardo Calado

Keyword(s):

Biomass Production ◽

Inorganic Nitrogen ◽

Inorganic Phosphorus ◽

Chemical Oxidation ◽

Culture Conditions ◽

Essential Elements ◽

Trophic Levels ◽

Shrimp Farming ◽

Hediste Diversicolor ◽

Arenicola Marina

AbstractThe bioremediation and biomass production of organic extractive organisms (polychaetes Arenicola marina, Hediste diversicolor and halophyte Salicornia ramosissima) was assessed in an integrated multi-trophic aquaculture (IMTA) framework. Culture trials were performed outdoors using the nutient rich effluent from a shrimp farm employing recirculated aquaculture systems. Similar bioremediation efficiencies were obtained in cultures using a single polyculture tank (1 T) or two trophic levels separated tanks (2 T; ≈ 0.3 and 0.6 m2 operational area, respectively), with a reduction of 74–87% for particulate organic matter (POM), 56–64% for dissolved inorganic nitrogen (DIN) and 60–65% for dissolved inorganic phosphorus (DIP). Hediste diversicolor adapted well to culture conditions, reaching densities up to 5.000 ind. m−2 (≈ 78–98 g m−2). Arenicola marina failed to cope with water temperature that exceeded the species thermal limits, displaying a survival < 10% (20 °C often pointed as the maximum thermal threshold for this species). Productivity of S. ramosissima with 1 T was about twice that obtained with 2 T (≈ 150–170 and ≈ 60–90 g FW m−2 edible aboveground biomass, respectively). The yellowish coloration of cultured plants was likely due to the chemical oxidation and rapid sand filtration pre-treatment applied to the brackish groundwater used in the aquaculture facility, that removed iron (and probably other essential elements). Overall, 1 T design combining H. diversicolor and S. ramosissima displayed the best bioremediation performance and biomass production, while also allowing reducing in half the operational area required to implement this IMTA framework.

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Picoplankton community structure before, during and after convection event in the offshore waters of the southern Adriatic Sea

Biogeosciences Discussions ◽

10.5194/bgd-10-17859-2013 ◽

2013 ◽

Vol 10 (11) ◽

pp. 17859-17892

Author(s):

M. Najdek ◽

P. Paliaga ◽

T. Šilović ◽

M. Batistić ◽

R. Garić ◽

...

Keyword(s):

Bacterial Community ◽

Biomass Production ◽

Inorganic Nitrogen ◽

Bacterial Community Composition ◽

Bacterial Biomass ◽

Cell Replication ◽

Large Area ◽

Metabolic Capacity ◽

Environmental Forcing ◽

Autotrophic Biomass

Abstract. This paper documents the picoplankton community's response to changes in oceanographic conditions in the period between October 2011 and September 2012 at two stations belonging to South Adriatic Pit (SAP). The recorded data include the community's abundance, composition, prokaryotic production rates and bacterial metabolic capacity. The aforementioned interval included an intense sea cooling with formation of exceptionally, record-breaking dense water. We documented an especially intense winter convection episode that completely diluted the core of Levantine intermediate waters (LIW) in a large area encompassing the SAP's center and its margin. During this convection event the whole picoplankton community had significantly higher abundances with a recorded picoeukaryotic peak at the SAP margin. In the post-convection phase in March prokaryotic heterotrophic production strongly increased in the entire SAP area (up to 50 times; 456.8 nM C day−1). The autotrophic biomass increase (up to 5 times; 4.86 μg L−1) and a disruption of a close correspondence between prokaryotic heterotrophic biomass production and cell replication rates were observed only in the center of the SAP, which was not under the influence of LIW. At the SAP's margin such an effect was attenuated by LIW, since the waters affected by LIW were characterized by decreased concentrations of dissolved inorganic nitrogen, decreased autotrophic biomasses and by increased bacterial biomass production balanced with cell replication rates as well as by the domination of Synechococcus among autotrophic picoplankton. Metabolic capacity was the lowest in spring when autotrophic biomass largely increased, while the highest levels found in the pre-convection phase (October 2011) suggests that the system was more oligotrophic before than after the convection event. Furthermore, we showed that metabolic capacity is a trait of bacterial community independent of environmental conditions and tightly linked to cell replication and substrate availability. On the other hand the bacterial community composition appears to be strongly influenced by physico-chemical characteristics of waters (e.g. temperature and nutrients) and environmental forcing (e.g. convection and LIW).

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