Industrial production of Phaeodactylum tricornutum for CO2 mitigation: biomass productivity and photosynthetic efficiency using photobioreactors of different volumes

2019 ◽  
Vol 31 (4) ◽  
pp. 2187-2196 ◽  
Author(s):  
Pedro M. Quelhas ◽  
Mafalda Trovão ◽  
Joana T. Silva ◽  
Adriana Machado ◽  
Tamára Santos ◽  
...  
Keyword(s):  
Industrial Production ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Efficiency ◽  
Biomass Productivity ◽  
Co2 Mitigation

Initial Investigations of a Shallow-Layer Algal Production System

1979 ◽  
Author(s):  
L. P. Raymond
Keyword(s):  
Photosynthetically Active Radiation ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Efficiency ◽  
Culture Media ◽  
Marine Diatom ◽  
Foam Fractionation ◽  
Total Solar Radiation ◽  
Full Potential ◽  
Active Radiation ◽  
Bio Engineering

A system for the intensive cultivation of the marine diatom Phaeodactylum tricornutum is described and evaluated. Unique features of the system include: (a) the incorporation of solar heat collection device which transmits only photosynthetically active radiation (PAR) to the growing culture; (b) the formulation of a new seawater enrichment medium that promotes physiological responses not previously observed in culture; and (c) the use of a foam fractionation device which separates microalgae, from the culture media, adds CO2-enriched air, and/or simultaneously recirculates the growing culture in shallow layers through an interconnecting series of hemicylindrical channels. The outdoor system demonstrated that very high ash-free dry weight yields of Phaeodactylum tricornutum are produced, a result of high photosynthetic efficiency. Actual yield over an eight-day period was equivalent to 39.57 ash-free dry tons/acre-year. Observed photosynthetic efficiency, based on photosynthetically active radiation incident upon the external surface of the system, is 13.1 percent, nearly three times the limit previously considered economically practical. The data indicate that greater yields may be expected using this system at locations receiveing higher insolation. A conservative projection is that 80 ash-free dry tons/acre-year will be realized in land regions receiveing 3 × 1010 Btu/acre-year total solar radiation. It is concluded that this system clearly warrants further investigation to determine its capacity to produce large and economical quantities of algal biomass for use as potential petroleum-fuel substitutes. The development of a comprehensive and systematic bio-engineering program is recommended to upgrade and evaluate the system to its full potential.

scholarly journals Bacterial diketopiperazines stimulate diatom growth and lipid accumulation

2021 ◽  
Author(s):  
John Sittmann ◽  
Munhyung Bae ◽  
Emily Mevers ◽  
Muzi Li ◽  
Andrew Quinn ◽  
...  
Keyword(s):  
Lipid Content ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Efficiency ◽  
Biofuel Production ◽  
Marine Diatom ◽  
Diatom Cell ◽  
Marine Microbes ◽  
Bacillus Cereus Group ◽  
Cell Lysate ◽  
High Lipid

Abstract Diatoms are photosynthetic microalgae that fix a significant fraction of the world’s carbon. Because of their photosynthetic efficiency and high-lipid content, diatoms are priority candidates for biofuel production. Here, we report that sporulating Bacillus thuringiensis and other members of the Bacillus cereus group, when in co-culture with the marine diatom Phaeodactylum tricornutum, significantly increase diatom cell count. Bioassay-guided purification of the mother cell lysate of B. thuringiensis led to the identification of two diketopiperazines (DKPs) that both stimulate P. tricornutum growth and increase its lipid content. These findings may be exploited to enhance P. tricornutum growth and microalgae-based biofuel production. As increasing numbers of DKPs are isolated from marine microbes, the work gives potential clues to bacterial-produced growth factors for marine microalgae.

scholarly journals Towards the Industrial Production of Omega-3 Long Chain Polyunsaturated Fatty Acids from a Genetically Modified Diatom Phaeodactylum tricornutum

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144054 ◽  
Author(s):  
Mary L. Hamilton ◽  
Joanna Warwick ◽  
Anya Terry ◽  
Michael J. Allen ◽  
Johnathan A. Napier ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Industrial Production ◽  
Phaeodactylum Tricornutum ◽  
Genetically Modified ◽  
Omega 3 ◽  
Long Chain

scholarly journals Scale-Up Cultivation of Phaeodactylum tricornutum to Produce Biocrude by Hydrothermal Liquefaction

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1072
Author(s):  
Irene Megía-Hervás ◽  
Alejandra Sánchez-Bayo ◽  
Luis Fernando Bautista ◽  
Victoria Morales ◽  
Federico G. Witt-Sousa ◽  
...  
Keyword(s):  
Biochemical Composition ◽  
Phaeodactylum Tricornutum ◽  
Nitrate Concentration ◽  
Scale Up ◽  
Biomass Productivity ◽  
Scaling Up ◽  
Hydrothermal Liquefaction ◽  
Co2 Injection ◽  
High Biomass ◽  
Initial Concentration

Phaeodactylum tricornutum is an interesting source of biomass to produce biocrude by hydrothermal liquefaction (HTL). Its biochemical composition, along with its biomass productivity, can be modulated according to this specific application by varying the photoperiod, the addition of CO2 or the variation of the initial nitrate concentration. The lab-scale culture allowed the production of a P. tricornutum biomass with high biomass and lipid productivities using a 18:6 h light:dark photoperiod and a specific CO2 injection. An initial concentration of nitrates (11.8 mM) in the culture was also essential for the growth of this species at the lab scale. The biomass generated in the scale-up photoreactor had acceptable biomass and lipid productivities, although the values were higher in the biomass cultivated at the lab scale because of the difficulty for the light to reach all cells, making the cells unable to develop and hindering their growth. The biocrudes from a 90-L cultivated microalga (B-90L) showed lower yields than the ones obtained from the biomass cultivated at the lab scale (B-1L) because of the lower lipid and high ash contents in this biomass. However, the culture scaling-up did not affect significantly the heteroatom concentrations in the biocrudes. A larger-scale culture is recommended to produce a biocrude to be used as biofuel after a post-hydrotreatment stage.

scholarly journals Thylakoid luminal θ-carbonic anhydrase critical for growth and photosynthesis in the marine diatom Phaeodactylum tricornutum

2016 ◽  
Vol 113 (35) ◽  
pp. 9828-9833 ◽  
Author(s):  
Sae Kikutani ◽  
Kensuke Nakajima ◽  
Chikako Nagasato ◽  
Yoshinori Tsuji ◽  
Ai Miyatake ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Efficiency ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Marine Diatom ◽  
Bisphosphate Carboxylase ◽  
Gfp Fusion Protein ◽  
Close Proximity ◽  
Esterase Activities

The algal pyrenoid is a large plastid body, where the majority of the CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) resides, and it is proposed to be the hub of the algal CO2-concentrating mechanism (CCM) and CO2 fixation. The thylakoid membrane is often in close proximity to or penetrates the pyrenoid itself, implying there is a functional cooperation between the pyrenoid and thylakoid. Here, GFP tagging and immunolocalization analyses revealed that a previously unidentified protein, Pt43233, is targeted to the lumen of the pyrenoid-penetrating thylakoid in the marine diatom Phaeodactylum tricornutum. The recombinant Pt43233 produced in Escherichia coli cells had both carbonic anhydrase (CA) and esterase activities. Furthermore, a Pt43233:GFP-fusion protein immunoprecipitated from P. tricornutum cells displayed a greater specific CA activity than detected for the purified recombinant protein. In an RNAi-generated Pt43233 knockdown mutant grown in atmospheric CO2 levels, photosynthetic dissolved inorganic carbon (DIC) affinity was decreased and growth was constantly retarded; in contrast, overexpression of Pt43233:GFP yielded a slightly greater photosynthetic DIC affinity. The discovery of a θ-type CA localized to the thylakoid lumen, with an essential role in photosynthetic efficiency and growth, strongly suggests the existence of a common role for the thylakoid-luminal CA with respect to the function of diverse algal pyrenoids.

scholarly journals Combined artificial high-silicate medium and LED illumination promote carotenoid accumulation in the marine diatom Phaeodactylum tricornutum

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Zhiqian Yi ◽  
Yixi Su ◽  
Paulina Cherek ◽  
David R. Nelson ◽  
Jianping Lin ◽  
...  
Keyword(s):  
Light Intensity ◽  
Phaeodactylum Tricornutum ◽  
Red Light ◽  
Monochromatic Light ◽  
Biomass Productivity ◽  
Marine Diatom ◽  
Light Illumination ◽  
Scale Production ◽  
Red Led ◽  
High Silicate

Abstract Background Diatoms, which can accumulate large amounts of carotenoids, are a major group of microalgae and the dominant primary producer in marine environments. Phaeodactylum tricornutum, a model diatom species, acquires little silicon for its growth although silicon is known to contribute to gene regulation and play an important role in diatom intracellular metabolism. In this study, we explored the effects of artificial high-silicate medium (i.e. 3.0 mM sodium metasilicate) and LED illumination conditions on the growth rate and pigment accumulation in P. tricornutum, which is the only known species so far that can grow without silicate. It’s well known that light-emitting diodes (LEDs) as novel illuminants are emerging to be superior monochromatic light sources for algal cultivation with defined and efficient red and blue lights. Results Firstly, we cultivated P. tricornutum in a synthetic medium supplemented with either 0.3 mM or 3.0 mM silicate. The morphology and size of diatom cells were examined: the proportion of the oval and triradiate cells decreased while the fusiform cells increased with more silicate addition in high-silicate medium; the average length of fusiform cells also slightly changed from 14.33 µm in 0.3 mM silicate medium to 12.20 µm in 3.0 mM silicate medium. Then we cultivated P. tricornutum under various intensities of red light in combination with the two different levels of silicate in the medium. Higher biomass productivity also achieved in 3.0 mM silicate medium than in 0.3 mM silicate medium under red LED light irradiation at 128 μmol/m2/s or higher light intensity. Increasing silicate reversed the down-regulation of fucoxanthin and chlorophyll a under high red-light illumination (i.e. 255 μmol/m2/s). When doubling the light intensity, fucoxanthin content decreased under red light but increased under combined red and blue (50:50) lights while chlorophyll a content reduced under both conditions. Fucoxanthin accumulation and biomass productivity increased with enhanced red and blue (50:50) lights. Conclusion High-silicate medium and blue light increased biomass and fucoxanthin production in P. tricornutum under high light conditions and this strategy may be beneficial for large-scale production of fucoxanthin in diatoms.

Microalgae with a truncated light-harvesting antenna to maximize photosynthetic efficiency and biomass productivity: Recent advances and current challenges

2021 ◽  
Vol 104 ◽  
pp. 83-91
Author(s):  
Vinod Kumar ◽  
Nishesh Sharma ◽  
Krishna Kumar Jaiswal ◽  
Mikhail S. Vlaskin ◽  
Manisha Nanda ◽  
...  
Keyword(s):  
Photosynthetic Efficiency ◽  
Light Harvesting ◽  
Biomass Productivity ◽  
Recent Advances ◽  
Light Harvesting Antenna

Industrial Output in the USSR and Russia, 1861—2012. Part II. Crises and Cycles

2013 ◽  
pp. 138-153 ◽  
Author(s):  
S. Smirnov
Keyword(s):  
Industrial Production ◽  
Turning Points ◽  
Russian Empire ◽  
Industrial Output ◽  
Production Index ◽  
Russian Industry ◽  
Industrial Production Index ◽  
Cyclical Volatility

Calculation of the aggregated "consensus" industrial production index has made it possible to date cyclical turning points and to measure the depth and length of the main industrial recessions in Russian Empire/USSR/Russia for the last century and a half. The most important causes of all these recessions are described. The cyclical volatility of Soviet/Russian industry is compared to that of American one.

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