scholarly journals Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry

2020 ◽  
Author(s):  
Tiago Taborda ◽  
Patrícia Moniz ◽  
Alberto Reis ◽  
Teresa Lopes da Silva
Keyword(s):  
Flow Cytometry ◽  
Low Cost ◽  
Crypthecodinium Cohnii ◽  
Dha Production

scholarly journals Infinity Flow: High-throughput single-cell quantification of 100s of proteins using conventional flow cytometry and machine learning

2020 ◽  
Author(s):  
Etienne Becht ◽  
Daniel Tolstrup ◽  
Charles-Antoine Dutertre ◽  
Florent Ginhoux ◽  
Evan W. Newell ◽  
...  
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
Single Cell ◽  
Low Cost ◽  
Expression Patterns ◽  
Cell Types ◽  
Cellular Heterogeneity ◽  
Supervised Machine Learning ◽  
Melanoma Metastasis ◽  
Immunologic Research

AbstractModern immunologic research increasingly requires high-dimensional analyses in order to understand the complex milieu of cell-types that comprise the tissue microenvironments of disease. To achieve this, we developed Infinity Flow combining hundreds of overlapping flow cytometry panels using machine learning to enable the simultaneous analysis of the co-expression patterns of 100s of surface-expressed proteins across millions of individual cells. In this study, we demonstrate that this approach allows the comprehensive analysis of the cellular constituency of the steady-state murine lung and to identify novel cellular heterogeneity in the lungs of melanoma metastasis bearing mice. We show that by using supervised machine learning, Infinity Flow enhances the accuracy and depth of clustering or dimensionality reduction algorithms. Infinity Flow is a highly scalable, low-cost and accessible solution to single cell proteomics in complex tissues.

scholarly journals Diagnostic accuracy and clinical utility of a simplified low cost method of counting CD4 cells with flow cytometry in Malawi: diagnostic accuracy study

BMJ ◽  
2007 ◽  
Vol 335 (7612) ◽  
pp. 190-190 ◽  
Author(s):  
Calman A MacLennan ◽  
Michael K P Liu ◽  
Sarah A White ◽  
Joep J G van Oosterhout ◽  
Felanji Simukonda ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Diagnostic Accuracy ◽  
Clinical Utility ◽  
Low Cost ◽  
Diagnostic Accuracy Study ◽  
Cd4 Cells ◽  
Accuracy Study

Design and Fabrication of Micro Hot Embossing Mold for Microfluidic Chip Used in Flow Cytometry

2007 ◽  
Vol 339 ◽  
pp. 246-251
Author(s):  
L.Q. Du ◽  
C. Liu ◽  
H.J. Liu ◽  
J. Qin ◽  
N. Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Chip ◽  
Low Cost ◽  
Hot Embossing ◽  
Microfluidic Chips ◽  
Metal Mold ◽  
Nickel Electroforming ◽  
Thick Photoresist ◽  
Nickel Base ◽  
Microfabrication Technology

Micro hot embossing mold of microfluidic chip used in flow cytometry is designed and microfabricated. After some kinds of microfabrication processes are tried, this paper presents a novel microfabrication technology of micro hot embossing metal mold. Micro metal mold is fabricated by low-cost UV-LIGA surface micro fabrication process using negative thick photoresist, SU-8. Different from other micro hot embossing molds, the micro mold with vertical sidewalls is fabricated by micro nickel electroforming directly on Nickel base. Based on the micro Nickel mold and automation fabrication system, high precision and mass-producing microfluidic chips have been fabricated and they have been used in flow cytometry

scholarly journals A customizable microscopy system for the automated quantification and characterization of multiple adherent cell types: an alternative to flow cytometry

2018 ◽  
Author(s):  
Vishwaratn Asthana ◽  
Yuqi Tang ◽  
Adam Ferguson ◽  
Pallavi Bugga ◽  
Anantratn Asthana ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Dynamic Range ◽  
Low Cost ◽  
Cell Types ◽  
Cell Counting ◽  
Adherent Cell ◽  
Optical Cell ◽  
Essential Components ◽  
Multiple Cell

Cell quantification assays are essential components of most biological and clinical labs. However, many currently available quantification assays, including flow cytometry and commercial cell counting systems, suffer from unique drawbacks that limit their overall efficacy. In order to address the shortcomings of traditional quantification assays, we have designed a robust, low-cost, automated optical cell cytometer that quantifies individual cells in a multiwell plate using tools readily available in most labs. Plating and subsequent quantification of various dilution series using the automated optical cytometer demonstrates the single-cell sensitivity, near-perfect R2 accuracy, and greater than 5-log dynamic range of our system. Further, the optical cytometer is capable of obtaining absolute counts of multiple cell types in one well as part of a co-culture setup. To demonstrate this ability, we recreated an experiment that assesses the tumoricidal properties of primed macrophages on co-cultured tumor cells as a proof-of-principle test. The results of the experiment reveal that primed macrophages display enhanced cytotoxicity towards tumor cells while simultaneously losing the ability to proliferate, an example of a dynamic interplay between two cell populations that our optical cytometer is successfully able to elucidate.

scholarly journals A customizable microscopy system for the automated quantification and characterization of multiple adherent cell types: an alternative to flow cytometry

2018 ◽  
Author(s):  
Vishwaratn Asthana ◽  
Yuqi Tang ◽  
Adam Ferguson ◽  
Pallavi Bugga ◽  
Anantratn Asthana ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Dynamic Range ◽  
Low Cost ◽  
Cell Types ◽  
Cell Counting ◽  
Adherent Cell ◽  
Optical Cell ◽  
Essential Components ◽  
Multiple Cell

Cell quantification assays are essential components of most biological and clinical labs. However, many currently available quantification assays, including flow cytometry and commercial cell counting systems, suffer from unique drawbacks that limit their overall efficacy. In order to address the shortcomings of traditional quantification assays, we have designed a robust, low-cost, automated optical cell cytometer that quantifies individual cells in a multiwell plate using tools readily available in most labs. Plating and subsequent quantification of various dilution series using the automated optical cytometer demonstrates the single-cell sensitivity, near-perfect R2 accuracy, and greater than 5-log dynamic range of our system. Further, the optical cytometer is capable of obtaining absolute counts of multiple cell types in one well as part of a co-culture setup. To demonstrate this ability, we recreated an experiment that assesses the tumoricidal properties of primed macrophages on co-cultured tumor cells as a proof-of-principle test. The results of the experiment reveal that primed macrophages display enhanced cytotoxicity towards tumor cells while simultaneously losing the ability to proliferate, an example of a dynamic interplay between two cell populations that our optical cytometer is successfully able to elucidate.

A novel integrated dual microneedle-microfluidic impedance flow cytometry for cells detection in suspensions

2017 ◽  
Vol 7 (3) ◽  
pp. 1513 ◽  
Author(s):  
Muhammad Asraf Mansor ◽  
Masaru Takeuchi ◽  
Masahiro Nakajima ◽  
Yasuhisa Hasegawa ◽  
Mohd Ridzuan Ahmad
Keyword(s):  
Flow Cytometry ◽  
Low Cost ◽  
Cost Effective ◽  
Electrical Measurement ◽  
Cell Detection ◽  
Cleaning Process ◽  
Effective Impedance ◽  
Pdms Microchannel ◽  
Flow Cytometry Detection ◽  
Core Functionality

In this study, a new, simple and cost-effective impedance detection of yeast cell concentration by using a novel integrated dual microneedle-microfluidic impedance flow cytometry was introduced. The reported method for impedance flow cytometry detection utilizes embedded electrode and probe in the microfluidic device to perform measurement of electrical impedance when a presence of cells at sensing area. Nonetheless, this method requires costly and complicatedly fabrication process of electrode. Furthermore, to reuse the fabricated electrode, it also requires intensive and tedious cleaning process. Due to that, a dual microneedle integrated at the half height of the microchannel for cell detection as well as for electrical measurement was demonstrated. A commercial available Tungsten needle was utilized as a dual microneedle. The microneedle was easy to be removed from the disposable PDMS microchannel and can be reused with the simple cleaning process, such as washed by using ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting the passing cells at sensing area. Therefore, this device is suitable for low cost medical and food safety screening and testing process in developing countries.

Flow Cytometry Applications in the Diagnosis of Myelodysplastic Syndrome (MDS): Analysis of 45 Cases.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4724-4724
Author(s):  
Patricia Font ◽  
Dolores Subira ◽  
Maria C. Martinez-Chamorro ◽  
Susana Castañon ◽  
Juan Lopez-Pascual ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Monoclonal Antibodies ◽  
Low Cost ◽  
Myeloid Cell ◽  
Normal Karyotype ◽  
High Sensitivity ◽  
Hematologic Malignancies ◽  
Potential Contribution ◽  
Flow Cytometric

Abstract Flow cytometry is a widely used method to study most of the hematologic malignancies. The utility of bone marrow immunophenotyping for the evaluation of patients with myelodisplastic syndrome (MDS) is currently being evaluated but most of these studies are based on the analysis of a large number of monoclonal antibodies. OBJECTIVE: To explore the potential contribution of flow cytometry to the diagnosis of MDS using a reduced panel of conjugated monoclonal antibodies (MoAb). PATIENTS AND METHODS: 45 bone marrow specimens from patients with a diagnosis of MDS based on morphologic and cytogenetic parameters (17 RA, 12 RARS, 5 MMCL, 9 RAEB, 2 RAEB-t), were analyzed by flow cytometry. In addition, 25 samples of bone marrow obtained from patients with cytopenias, but no diagnosis of MDS, were also studied. The panel of MoAb used was designed to identify abnormalities in the differentiation pathways of erithroid (CD71 FITC/Gly A PE/CD45 PC5) and myeloid lineage (CD 16 FITC/CD11b PE/CD13 PC5) as well as the presence of specific aberrant features in the CD34+ myeloid cell population (TdT FITC/CD7 PE/CD34 PC5). All samples were analized by two independet observers. To establish the diagnosis of MDS by flow cytometry, it was necessary to describe either immunophenotypic abnormalities in both myeloid and erithroid lineages or abnormalities in only one lineage plus description of more than 5% of CD34+ cells or abnormalities in one lineage plus description of aberrant features in the CD34+ population, regardless of its percentage. RESULTS: In 43 of the 45 samples analyzed, flow cytometric criteria of MDS were described. Only two cases (1 RARS with normal karyotype and 1 RA with complex cytogenetics) were considered normal according to the immunophenotypic criteria (95% sensivity). Regarding the cohort control, 4 samples had flow cytometric criteria of MDS; 11 samples showed isolated antigenic aberrancies in the erithroid differentiation and the 11 samples left were considered as normal. CONCLUSIONS: Flow cytometry may be a useful tool in the diagnosis of MDS, even analysing only two hemopoietic cell lineages. The reduced panel of MoAb described here can be widely used, is easy to be applied and shows a high sensitivity and a low cost. However, the finding of isolated immunophenotypic abnormalities in some patients without cytologic data of MDS seems to negatively influence the specificity of the technique. Nevertheless, those cases presenting an abnormal immunophenotype and normal morphology should be carefully monitored during their ulterior follow-up.

Sign in / Sign up

Export Citation Format

Share Document