scholarly journals Cytotoxicity of doxorubicin conjugated with C60 fullerene. Structural and in vitro studies

2019 ◽  
Vol 30 (6) ◽  
pp. 2327-2338
Author(s):  
Kamila Butowska ◽  
Witold Kozak ◽  
Magdalena Zdrowowicz ◽  
Samanta Makurat ◽  
Michał Rychłowski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Aqueous Solution ◽  
Fluorescence Spectra ◽  
C60 Fullerene ◽  
Absorption And Fluorescence Spectra ◽  
H Nmr ◽  
Low Toxicity ◽  
Pass Through ◽  
The Impact

Abstract Conjugating an anticancer drug of high biological efficacy but large cytotoxicity with a “transporting” molecule of low toxicity constitutes a valuable approach to design safe drug delivery system. In the present study, doxorubicin (DOX) a drug of large cardiotoxicity was chemically conjugated to a C60-fullerene. The synthesized molecule, a fullerene-doxorubicin conjugate (Ful-DOX), was characterized using the 1H NMR and MALDI TOF mass spectrometry. The absorption and fluorescence spectra and dynamic light scattering of the conjugate were recorded in an aqueous solution, while the impact on viability of several cancer cell lines of the free DOX and the conjugate was compared using the SRB and WST-1 assays. A low antiproliferative activity of the conjugate as compared to the free DOX is a consequence of the presence of fullerene moiety in the former, which is also responsible for the conjugate aggregation in an aqueous solution. Unlike free DOX, these aggregates cannot pass through the nuclear membrane (as demonstrated by the confocal microscopy measurements), which makes them marginally cytotoxic.

Synthesis, Characterization and Photophysical Properties of Rhenium(I) Complexes Containing 4,5-Diazafluorene Ligan

2011 ◽  
Vol 239-242 ◽  
pp. 2612-2615
Author(s):  
Jie Xiu Wang ◽  
Feng Zhao
Keyword(s):  
Charge Transfer ◽  
Fluorescence Spectra ◽  
Photophysical Properties ◽  
Absorption And Fluorescence Spectra ◽  
Rhenium Complexes ◽  
H Nmr ◽  
Ligand Charge Transfer

Two related tricarbonyl rhenium complexes with the formula of [Re(CO)3(L)Cl], where L=4,5-diazafluoren-9-one (Dafo-Re) and 9-(phenylamino)-4,5-diazafluoren (PADF-Re) were successfully synthesized with the aim to explore the effect of the geometry of Dafo on Rhenium(I) coordination, and characterized by1H NMR. Photophysical behaviors are investigated by UV–vis absorption and Fluorescence spectra. The two complexes Dafo-Re and PADF-Re show metal-to-ligand charge transfer absorptions at ca.391nm, ca.394 nm and emissions at ca.492nm, ca.470nm, respectively.

Synthesis, Electronic States, and Electroluminescent Properties of Aluminum Complex Based on Mixed Ligands with 8-Hydroxyquinolinate and Acetylacetone

2011 ◽  
Vol 306-307 ◽  
pp. 358-361
Author(s):  
Liu Qing Chen ◽  
Chun Yan Sun ◽  
Xu Guang Liu ◽  
Bing She Xu
Keyword(s):  
Fluorescence Spectra ◽  
Orbital Energy ◽  
Computational Studies ◽  
Absorption And Fluorescence Spectra ◽  
Aluminum Complex ◽  
Analysis Techniques ◽  
H Nmr ◽  
Mixed Ligands ◽  
Organic Electroluminescent ◽  
Electron Transport Properties

We report the synthesis, photophysical investigation, computational studies and electroluminescent(EL) properties of an organic electroluminescent material-Bis(8-hydroxyQuino line) acetylacetone aluminum (Alq2A). It is characterized by 1H NMR, IR and elemental analysis techniques. The absorption and fluorescence spectra of various Alq2A systems including solution in ethanol, powder and thin film on quartz (thickness of 50nm) have also been systematically evaluated. Analysis of the electronic structure of Alq2A calculated by quantum chemical calculations reveals a localization of orbital and the distribution of orbital energy. The results from EL experiments indicate that Alq2A has good electron transport properties as compared with tris(8-hydroxyquinolinato) aluminum (Alq3). Thus, Alq2A is considered to be a superior emitter and electron transporting material for display application compared with Alq3.

scholarly journals Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
CAO Haiyan ◽  
QIN Xiude ◽  
LIU Chen ◽  
ZHAO Xinzhe ◽  
MA Yuhui ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Pharmacological Effects ◽  
Absorption And Fluorescence Spectra ◽  
Hydroxysafflor Yellow A ◽  
T24 Cells ◽  
Fluorescence Sensitization ◽  
In Cells

As the main active ingredient in Chinese medicine safflower, hydroxysafflor yellow A (HSYA) has multiple pharmacological effects. In the work, the absorption and fluorescence spectra of HSYA under different environmental conditions (such as acidity, temperature, ions, viscosity, and surfactant) were investigated. The fluorescence intensity of HSYA varied greatly with acidity, temperature, viscosity, and surfactant, but was less affected by common cations and anions. Among various surfactants, we found that borax can significantly enhance the HSYA fluorescence intensity, and thus, a borax-HSYA sensitization system for HSYA fluorescence was established. In the optimized sensitization system, the fluorescence intensity of HSYA increased by 20 times and showed a good linearity with HSYA concentrations in the range of 0∼10 μM with a detection limit of 8 nM. The borax-HSYA sensitization system is nontoxic to T24 cells and mice and can be used for the fluorescence imaging of HSYA in cells, thereby providing an effective method for analyzing HSYA in vitro and monitoring its metabolism in cells.

NOVEL POLYETHYLENIMINE NANOGELS AS POTENTIAL GENE CARRIERS PRODUCED VIA PHOTOCHEMISTRY IN SURFACTANT-FREE AQUEOUS SOLUTION

2006 ◽  
Vol 05 (06) ◽  
pp. 753-756 ◽  
Author(s):  
DONGMEI XU ◽  
JIAHUI YU ◽  
YONGBIAO LIU ◽  
HANWEN SUN ◽  
JINGYING XU ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Transfection Efficiency ◽  
In Vitro Tests ◽  
Gene Carriers ◽  
Polymeric Particles ◽  
Potential Applications ◽  
Novel Method ◽  
Low Toxicity

Cationic polymer nanogels, positively-charged submicrometer polymeric particles that swell in water, have attracted an increasing research attentions in recent years because of their potential applications as gene carriers. In this paper, we report a novel method to synthesize polyethylenimine (PEI) nanogels with sizes ranging from 80 nm to 200 nm via UV irradiation at room temperature in aqueous solution without adding any kind of surfactants. The morphology of the nanoparticles is determined to be spherical. The nanogels are of high stability, high transfection efficiency, low toxicity and low immunogenicity, as having been confirmed by in vivo tests with mice as an animal model, and by in vitro tests with human lung and liver cancer cells as well.

Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos

Zygote ◽  
2014 ◽  
Vol 23 (5) ◽  
pp. 732-741 ◽  
Author(s):  
Beatriz C. S. Leão ◽  
Nathália A. S. Rocha-Frigoni ◽  
Elaine C. Cabral ◽  
Marcos F. Franco ◽  
Christina R. Ferreira ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lipid Profile ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Maldi Ms ◽  
Charge Ratio ◽  
Alkyl Ether ◽  
Bovine Embryos ◽  
The Impact

SummaryThis study aimed to evaluate the impact of vitrification on membrane lipid profile obtained by mass spectrometry (MS) of in vitro-produced bovine embryos. Matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS) has been used to obtain individual embryo membrane lipid profiles. Due to conditions of analysis, mainly membrane lipids, most favorably phosphatidylcholines (PCs) and sphingomyelins (SMs) have been detected. The following ions described by their mass-to-charge ratio (m/z) and respective attribution presented increased relative abundance (1.2–20×) in the vitrified group: 703.5 [SM (16:0) + H]+; 722.5 [PC (40:3) + Na]+; 758.5 [PC (34:2) + H]+; 762.5 [PC (34:0) + H]+; 790.5 [PC (36:0) + H]+ and 810.5 [PC (38:4) + H]+ and/or [PC (36:1) + Na]+. The ion with a m/z 744.5 [PCp (34:1) and/or PCe (34:2)] was 3.4-fold more abundant in the fresh group. Interestingly, ions with m/z 722.5 or 744.5 indicate the presence of lipid species, which are more resistant to enzymatic degradation as they contain fatty acyl residues linked through ether type bonds (alkyl ether or plasmalogens, indicated by the lowercase ‘e’ and ‘p‘, respectively) to the glycerol structure. The results indicate that cryopreservation impacts the membrane lipid profile, and that these alterations can be properly monitored by MALDI-MS. Membrane lipids can therefore be evaluated by MALDI-MS to monitor the effect of cryopreservation on membrane lipids, and to investigate changes in lipid profile that may reflect the metabolic response to the cryopreservation stress or changes in the environmental conditions.

Effect of Co-Solvents on Physicochemical Properties and Stability of Areca Nut Extract Loaded Nanoemulsions

2020 ◽  
Vol 862 ◽  
pp. 40-45
Author(s):  
Jayanant Iemsam-Arng ◽  
Saowaluk Jangkena ◽  
Kunat Suktham ◽  
Ratjika Wongwanakul ◽  
Onuma Unger ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Water Solubility ◽  
Human Skin Fibroblast ◽  
Areca Nut ◽  
Polyethylene Glycol 400 ◽  
Normal Human Skin ◽  
Normal Human ◽  
Low Toxicity ◽  
The Impact

Areca nut extract provides a variety of pharmacological effects that are beneficial for skincare applications. A nanoemulsion of areca nut extract was developed to mask the intense color and to improve the water solubility of the extract. This work studied the impact of a co-solvent on the characteristics and stability of the nanoemulsion. Our former optimized nanoemulsion was modified by adding a common co-solvent, propylene glycol or polyethylene glycol 400 (PEG400), to the formula. Phase separation, particle characteristics, antioxidant activity, in vitro cytotoxicity, and stability of the modified nanoemulsion were evaluated. This work has shown the successful encapsulation of areca nut extract with a great improvement of stability, well-maintained antioxidant activity and low toxicity on normal human skin fibroblast.

Cucurbit[7]uril host-guest complexes with cationic bis(4,5-dihydro-1H-imidazol-2-yl) guests in aqueous solution

2006 ◽  
Vol 84 (6) ◽  
pp. 905-914 ◽  
Author(s):  
D Saroja N Hettiarachchi ◽  
Donal H Macartney
Keyword(s):  
Mass Spectrometry ◽  
Aqueous Solution ◽  
Nmr Spectroscopy ◽  
Stability Constants ◽  
Electrospray Mass Spectrometry ◽  
Hydrophobic Core ◽  
Guest Complex ◽  
H Nmr ◽  
Host Guest Complex ◽  
Proton Resonances

The host–guest interactions between cucurbit[7]uril and a series of novel cationic bis(4,5-dihydro-1H-imidazol-2-yl)arene and 1-(4,5-dihydro-1H-imidazol-2-yl)- and 1,3-bis(4,5-dihydro-1H-imidazol-2-yl)-adamantane guests have been investigated in aqueous solution using UV–vis and NMR spectroscopy, and electrospray mass spectrometry. With the exception of the 1,3-bis(4,5-dihydro-1H-imidazol-2-yl)adamantane (which binds externally to the CB[7]), these guests form very stable inclusion complexes with slow exchange on the 1H NMR timescale. The direction and magnitude of the complexation-induced shifts (CIS) in the proton resonances of the guests are indicative of the residence of the hydrophobic core of the guest within the CB[7] cavity and the charged 4,5-dihydro-1H-imidazol-2-yl units outside the cavity adjacent to the carbonyl-lined portals of the host. The CIS values and the inclusion stability constants have been correlated with the nature of the guest core and with the distance between the charges on the terminal 4,5-dihydro-1H-imidazol-2-yl rings.Key words: cucurbit[7]uril, host–guest complex, dihydroimidazolyl, inclusion stability constants.

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