scholarly journals Morpho-anatomical and biochemical changes associated with rooting of micropropagated ninebark cuttings

2021 ◽  
Author(s):  
Katarzyna Jagiełło-Kubiec ◽  
Karolina Nowakowska ◽  
Aleksandra Józefina Łukaszewska ◽  
Andrzej Pacholczak
Keyword(s):  
Woody Plants ◽  
Culture Medium ◽  
Root Formation ◽  
Cost Effective ◽  
Biochemical Changes ◽  
Limiting Factor ◽  
Naphthalene Acetic Acid ◽  
Physocarpus Opulifolius ◽  
Ornamental Shrub

AbstractNinebark (Physocarpus opulifolius) is an attractive ornamental shrub with poor rooting characteristics in some cultivars, which is a limiting factor in commercial production This study was designed to optimize rooting conditions of ninebark cuttings and to observe the effect of exogenous auxin IBA on some morpho-anatomical and biochemical changes associated with rhizogenesis in the in vitro conditions. Both auxins under study: the indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) gave comparable effects but the combination of ½ MS + 1 mg·L−1 IBA was the most cost effective for all rooting parameters. Anatomical changes at the cuttings’ bases during root formation were typical for woody plants and they were accelerated by auxin in the culture medium. High levels of the endogenous indole acid and hydrogen peroxide were temporarily associated with intensive cell divisions in cuttings, and the polyphenolic acid contents kept increasing during rooting above the initial levels and those in controls.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

scholarly journals Long-term in Vitro Regeneration of Hamelia patens

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873G-874
Author(s):  
D. Sankhla ◽  
T.D. Davis ◽  
N. Sankhla ◽  
A. Upadhyaya
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Prolific Shoot ◽  
Ornamental Shrub

This report describes an efficient in vitro regeneration protocol for H. patens (firebush), a heat-tolerant ornamental shrub native to tropical and subtropical America. Shoot cultures were initially established using shoot tips placed on MS-revised medium containing 2.3 μM 2,4-D, 2.3 μM kinetin, and 0.25% polyvinylpyrrolidone. Other types of explants (nodal and internodal segments, leaf pieces, floral buds) did not regenerate shoots when placed on this medium. Two-month-old plantlets derived from the shoot tips were subcultured on MS medium supplemented with 0.5 μM thidiazuron (TDZ), and within 3 to 4 weeks, some callus was produced at the root–shoot junction. When this callus, with a small portion of the root and shoots, was placed on MS medium with 0.05 μM TDZ and 0.01 μM ABA, prolific shoot formation occurred within 3 to 4 weeks followed by root formation. By regular subculturing every 5 to 6 weeks, hundreds of plantlets have been obtained over the past 3 years with no apparent decline in regeneration potential. Addition of activated charcoal (0.5%) to the culture medium has greatly improved growth of the plantlets.

scholarly journals In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

2017 ◽  
pp. 50-55
Author(s):  
Khalid Lagram ◽  
Mohamed Ben El Caid ◽  
Souad El Aaouam ◽  
Mohamed Lachheb ◽  
Abdelhamid El Mousadik ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Crocus Sativus ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Male Sterile ◽  
Indirect Organogenesis ◽  
Crocus Sativus L

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

scholarly journals Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 774
Author(s):  
Nastassja Kimberlly Lima ◽  
Eloisa Schneider Da Silva ◽  
Rayane Monique Sete Da Cruz ◽  
Pedro Henrique Riboldi Monteiro ◽  
Glacy Jaqueline Da Silva
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Aerial Part ◽  
Large Scale ◽  
Dry Mass ◽  
Fresh Mass ◽  
Limiting Factor ◽  
Naphthalene Acetic Acid ◽  
In Vitro Cultivation

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

scholarly journals In vitro sensivity of fig plantlets to gamma rays

2009 ◽  
Vol 66 (4) ◽  
pp. 540-542 ◽  
Author(s):  
Ester Alice Ferreira ◽  
Moacir Pasqual ◽  
Augusto Tulmann Neto
Keyword(s):  
Gamma Rays ◽  
Aerial Part ◽  
Culture Medium ◽  
Root Formation ◽  
Low Cost ◽  
Breeding Programs ◽  
Growth Room ◽  
Bud Position ◽  
Natural Pollination

Fig breeding programs through conventional methods are rare in many countries, e.g. Brazil, since the wasp Blastophaga psenes, which is responsible for the natural pollination, is not present. For these cases a low cost alternative for the breeding program is the induction of physical mutagenesis by radiation. The sensivity of fig explant buds of different sizes to gamma radiation were evaluated. Fig plantlets "Roxo de Valinhos" already established in vitro were classified by size in 2.5 to 4.5 cm, 5 to 9 7 cm and 8 to 10 cm long, and irradiated with: 10, 20, 30, 40 and 50 Gy doses. After irradiation each plantlet was cut in pieces containing one-bud and transferred to WPM culture medium, according to the bud position: medium and apical. Explants were grown in a growth room for 90 days when, explant mortality, root formation, height of aerial part, number of buds and plantlet weight were evaluated. Doses of up to 50 Gy do not cause plantlet death and that doses larger than 30 Gy inhibit root formation. Therefore, the 30 Gy dose may be recommended for the irradiation of fig plantlets larger than 2.5 cm.

Callose-synthesizing enzymes as membrane proteins of Betula protoplasts secrete bundles of β-1,3-glucan hollow fibrils under Ca2+-rich and acidic culture conditions

Holzforschung ◽  
2020 ◽  
Vol 74 (8) ◽  
pp. 725-732
Author(s):  
Shintaro Matsuo ◽  
Satomi Tagawa ◽  
Yudai Matsusaki ◽  
Yuri Uchi ◽  
Tetsuo Kondo
Keyword(s):  
Membrane Proteins ◽  
Woody Plants ◽  
Culture Medium ◽  
Culture Conditions ◽  
White Birch ◽  
High Concentration ◽  
Callose Synthase ◽  
Insight Into

AbstractPreviously, it was reported that plant protoplasts isolated from Betula platyphylla (white birch) callus secreted bundles of hollow callose fibrils in acidic culture medium containing a high concentration of calcium ions (Ca2+). Here, the callose synthase was characterized from in situ and in vitro perspectives. Localization of callose synthases at the secreting site of callose fiber was indicated from in situ immunostaining observation of protoplasts. For in vitro analyses, membrane proteins were extracted from membrane fraction of protoplasts with a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) treatment. The CHAPS extract aggregated in the presence of a high concentration of Ca2+, suggesting that Ca2+ may promote the arrangement of callose synthases in the plasma membrane. The callose synthase activity was dependent on pH and Ca2+, similar to the callose synthase of Arabidopsis thaliana. However, the synthesized fibril products were longer than those produced by callose synthases of herbaceous plants. This is the first insight into the specific properties of callose synthases of woody plants that secrete of callose hollow fibers.

scholarly journals The influence of Cinnamomum zeylanicum in plant tissue culture experiments of Gardenia jasmenoides Ellis

2013 ◽  
Vol 10 (4) ◽  
pp. 1102-1107
Author(s):  
Baghdad Science Journal
Keyword(s):  
Root Formation ◽  
Culture Media ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Alcoholic Extract ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Cinnamomum Zeylanicum ◽  
Positive Effect

Cinnamon plant is considered one of important medicinal plants because it is rich with many active compounds. This research is aimed to study possible effects of extract in culture media of Gardenia jasmenoides. Alcoholic extract was prepared from the bark of cinnamon at different concentrations (0.0, 1.0, 2.0) mg/L, then added to culture media to notice the effect of these concentrations on the growth and development of tissues and organs of Gardenia jasmenoides Ellis in vitro. Results showed the positive effect of increasing callus fresh weight and shoot proliferation from single nodes with presence of plant regulators, 5.0 mg/L Naphthalene acetic acid (NAA) and 3.0 mg/L Benzyl adenine (BA). Results showed that extract has a slight effect on root formation with the presence of plant regulators or when it is alone.

scholarly journals In Vitro Adventitious Rooting of Carrizo Citrange Microshoots

HortScience ◽  
2010 ◽  
Vol 45 (6) ◽  
pp. 988-990 ◽  
Author(s):  
Almudena Montoliu ◽  
Aurelio Gómez-Cadenas ◽  
Rosa M. Pérez-Clemente
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Basal Medium ◽  
Poncirus Trifoliata ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Carrizo Citrange

The objective of this work was to develop an efficient in vitro rooting protocol for one of the most commercially used citrus rootstocks in Spain, Carrizo citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.). Single-node cuttings taken from greenhouse-grown plants were cultured in petri dishes containing basal Murashige and Skoog medium. Shoots from nodal stem segments were excised and cultured in a multiplication medium (basal medium supplemented with 1.8 μM 6-benzylaminopurine) to promote the development of axillary buds. Individual shoots (15 mm long) were treated with different hormones at several concentrations for root induction evaluations. The addition of activated charcoal (AC) to the culture medium was also explored. The addition of auxins to the culture medium enhanced rooting percentage. Optimal results were obtained when 1-naphthalene acetic acid (10.8 μM) and gibberellic acid (0.3 μM) were added to the culture medium. The addition of AC to the rooting medium resulted in negative effects on the percentage of rooted shoots but had a positive effect on number of roots per rooted shoot. Chemical names used: activated charcoal (AC); 6-benzylaminopurine (BA); 1-naphthalene acetic acid (NAA); gibberellic acid (GA3); indole-3-butyric acid (IBA)

scholarly journals MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES

2015 ◽  
Vol 39 (2) ◽  
pp. 305-314 ◽  
Author(s):  
Luísa Maria Silveira de Almeida ◽  
Leandro Elias Morais ◽  
Cristiano Ferrara de Resende ◽  
Virgínia Fernandes Braga ◽  
Paula da Fonseca Pereira ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Native Species ◽  
Culture Medium ◽  
Anthropogenic Activities ◽  
Woody Species ◽  
Naphthalene Acetic Acid ◽  
Culture Development ◽  
Shoot Propagation ◽  
Indole 3 Acetic Acid

The objective of this work was to establish an efficient protocol for in vitro multiplication and rooting, as well as ex vitroacclimatization of Aegiphila verticillata, a woody species found in Brazilian rocky fields. Aseptic cultures were established by seeds and two multiplication analyses were performed. In the first, we employed 6-benzylaminopurine (BAP – 0, 2.5, 5 and 7.5 μM) + α-naphthalene acetic acid (NAA – 0, 0.2, 0.4 and 0.6 μM) and, in the second, were studied adenine sulfate, kinetin and thidiazuron (0, 5, 7.5, 10 and 12.5 μM). After 90 days, we assessed the quantitative and qualitative shoot propagation. There were more than 90% seed germination and low contamination (2%). In multiplication phase, the culture medium that promoted the best quantitative and qualitative culture development was supplemented with 7.5 μM BAP + 0.4 μM NAA. In the rooting assay, were used NAA, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) (0, 0.1, 0.2, 0.3 or 0.4 μM). After 90 days, the root number and rooting quality were evaluated. In this analysis, differences were not found between the control and the other treatments. Rooted plantlets were acclimatized in styrofoam trays for 30 days, after which they were transferred to pots in the greenhouse. Only 3% of the plants subjected to initial acclimatization died and 70% of the plants transferred to the field conditions survived and showed normal development. The results founded in this work are the first involving in vitro propagation and ex vitroacclimatization of Aegiphila verticillata and provide a continuous supply of this medicinal native species, endangered due anthropogenic activities.

Sign in / Sign up

Export Citation Format

Share Document