In-vitro anthelminthic activity of crude aqueous extracts of Aloe ferox, Leonotis leonurus and Elephantorrhiza elephantina against Haemonchus contortus

2009 ◽  
Vol 42 (2) ◽  
pp. 301-307 ◽  
Author(s):  
Viola Maphosa ◽  
Patrick J. Masika ◽  
Edmund S. Bizimenyera ◽  
J. N. Eloff
Keyword(s):  
Haemonchus Contortus ◽  
Aqueous Extracts ◽  
Anthelminthic Activity ◽  
Aloe Ferox ◽  
Leonotis Leonurus

In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep

2012 ◽  
Vol 87 (2) ◽  
pp. 174-179 ◽  
Author(s):  
M. Ahmed ◽  
M.D. Laing ◽  
I.V. Nsahlai
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Allium Sativum ◽  
Anthelmintic Activity ◽  
Ananas Comosus ◽  
Lespedeza Cuneata ◽  
Aloe Ferox ◽  
Ethanol Extracts

AbstractEthanol extracts of 25 plant species were screened for anthelmintic effects against Haemonchus contortus. Ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°C with control cultures which were treated with ethanol for 48 h. Five plants with high efficacies (Ananas comosus, Aloe ferox, Allium sativum, Lespedeza cuneata and Warburgia salutaris) were selected from the first screening for further investigation using ethanol, dichloromethane and water extracts at four concentrations (2.5, 5, 10 and 20%). Ethanol was the most effective solvent. Larval counts decreased with increasing extract concentrations, of which 10 and 20% had similar effects. Lespedeza cuneata caused more than 70% mortality at all concentrations. However, there remains a need to assess in vivo efficacy of these plants.

In vitro screening of 51 birdsfoot trefoil (Lotus corniculatus L.; Fabaceae) strains for anti-parasitic effects against Haemonchus contortus

Parasitology ◽  
2019 ◽  
Vol 146 (6) ◽  
pp. 828-836 ◽  
Author(s):  
Carly D. Barone ◽  
Anne M. Zajac ◽  
Sarah M. Ferguson ◽  
Rebecca N. Brown ◽  
Jess D. Reed ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Secondary Compounds ◽  
Lotus Corniculatus ◽  
Birdsfoot Trefoil ◽  
Aqueous Extracts ◽  
Egg Hatching ◽  
Parasitic Activity ◽  
Parasitic Effects

AbstractSecondary plant compounds have shown bioactivity against multi-drug resistant Haemonchus contortus in small ruminants. This study screened 51 strains of birdsfoot trefoil (BFT, Lotus corniculatus) crude aqueous extracts (BFT-AqE) for anti-parasitic activity in vitro against egg hatching, and of those 51 strains, 13 were selected for further testing of motility of first (L1) and third stage (L3) larvae, and exsheathment of L3. Proanthocyanidin content ranged between 1.4 and 63.8 mg PAC g−1 powder across the 51 BFT strains. When tested against egg hatching, 21 of the 51 aqueous extracts had an EC50 of 1–2 mg powder mL−1, 70% of the strains were >90% efficacious at 6 mg powder mL−1 and 11 of the strains were 100% efficacious at 3 mg powder mL−1 BFT-AqE. Across the 13 strains tested against L3, efficacy ranged from 0 to 75% exsheathment inhibition, and 17 to 92% L3 motility inhibition at a concentration of 25 mg powder mL−1 BFT-AqE. There was no correlation between the PAC content of BFT powders and the anti-parasitic activity of aqueous extracts, therefore other secondary compounds may have contributed to the observed anti-parasitic effects. Further testing of BFT using bioactivity-driven fractionation and screening of BFT populations for the identified anti-parasitic compounds is needed.

scholarly journals Anthelminthic Activity of <i>Moringa oleifera</i> Leaf Extracts Evaluated <i>in Vitro</i> on Four Developmental Stages of <i>Haemonchus contortus</i> from Goats

2014 ◽  
Vol 05 (11) ◽  
pp. 1702-1710 ◽  
Author(s):  
Gertrude Mbogning Tayo ◽  
Josué Wabo Poné ◽  
Marie Claire Komtangi ◽  
Jeannette Yondo ◽  
Alidou Marc Ngangout ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Moringa Oleifera ◽  
Developmental Stages ◽  
Leaf Extracts ◽  
Anthelminthic Activity

scholarly journals IN VITRO ANTHELMINTIC ACTIVITY OF ABUTILON THEOPHRASTI MEDIK. (MALVACEAE) AGAINST EGGS AND L3 LARVAE OF HAEMONCHUS CONTORTUS

2019 ◽  
pp. 205-207
Author(s):  
MUSHEERUL HASSAN ◽  
MIR RA ◽  
TRIPTI JOSHI ◽  
FIRDOUS AHMAD YATOO ◽  
HUMA HABIB
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Abutilon Theophrasti ◽  
Aqueous Extracts ◽  
Egg Hatch ◽  
Anthelmintic Efficacy ◽  
Dependent Inhibition ◽  
Hexane Extracts ◽  
Extraction Solvents

Objective: The present study was carried out to assess the in vitro anthelmintic activity of Abutilon theophrasti stem extracts. Methods: Simple maceration was employed for extraction. Solvents such as methanol, water, and hexane were used. Egg hatch test (EHT) and larval motility test were employed to check the anthelmintic activity of crude extracts. Concentrations of 500, 250, 125, 62.5, and 31.25 mg/ml were made. Levamisole and distilled water served as control, respectively. Results: All selected extracts displayed concentration-dependent inhibition except aqueous extracts. At higher concentration (500 mg/ml), stem extracts (methanol, aqueous, and ethanol) showed 74.39%, 72.5 and 70.03% of efficacy in EHT, respectively (p≤0.05). Meanwhile, inhibition of larval motility was seen higher with inhibition percentage of 79.79, 75.65, and 71.28 by methanolic, aqueous, and hexane extracts, respectively (p≤ 0.05). Conclusion: The presents study suggests active principles having anthelmintic efficacy in Abutilon theopharsti stem.

Effect of Jatropha curcas Latex on L3 Haemonchus contortus Larval Motility

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous
Keyword(s):  
Larvicidal Activity ◽  
Jatropha Curcas ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Small Ruminants ◽  
Gastrointestinal Nematodes ◽  
Dose Dependent ◽  
Plants Extract

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.

Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate

Acta Tropica ◽  
2021 ◽  
Vol 217 ◽  
pp. 105869
Author(s):  
Vivien Patricia Garbin ◽  
Beatriz Munguía ◽  
Jenny Carolina Saldaña ◽  
Cícero Deschamps ◽  
Roger Raupp Cipriano ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Chemical Characterization ◽  
Anthelmintic Activity ◽  
Citrus Bergamia

In vitro anthelmintic activity of Dennettia tripetala G. Baker (Annonaceae) Fruits against Haemonchus contortus

2021 ◽  
Author(s):  
Rachel A. Nwosu ◽  
Mohammed M. Suleiman ◽  
Hussaina J. Makun ◽  
Matthew P. Ameh ◽  
Manji A. Shetshak ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Dennettia Tripetala

scholarly journals Treatment with Uncaria tomentosa Promotes Apoptosis in B16-BL6 Mouse Melanoma Cells and Inhibits the Growth of B16-BL6 Tumours

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1066
Author(s):  
Ali Zari ◽  
Hajer Alfarteesh ◽  
Carly Buckner ◽  
Robert Lafrenie
Keyword(s):  
Tumour Size ◽  
Melanoma Cells ◽  
Tunel Staining ◽  
Aqueous Extracts ◽  
Ki 67 ◽  
Uncaria Tomentosa ◽  
Mouse Melanoma ◽  
Anti Cancer

Uncaria tomentosa is a medicinal plant native to Peru that has been traditionally used in the treatment of various inflammatory disorders. In this study, the effectiveness of U. tomentosa as an anti-cancer agent was assessed using the growth and survival of B16-BL6 mouse melanoma cells. B16-BL6 cell cultures treated with both ethanol and phosphate-buffered saline (PBS) extracts of U. tomentosa displayed up to 80% lower levels of growth and increased apoptosis compared to vehicle controls. Treatment with ethanolic extracts of Uncaria tomentosa were much more effective than treatment with aqueous extracts. U. tomentosa was also shown to inhibit B16-BL6 cell growth in C57/bl mice in vivo. Mice injected with both the ethanolic and aqueous extracts of U. tomentosa showed a 59 ± 13% decrease in B16-BL6 tumour weight and a 40 ± 9% decrease in tumour size. Histochemical analysis of the B16-BL6 tumours showed a strong reduction in the Ki-67 cell proliferation marker in U. tomentosa-treated mice and a small, but insignificant increase in terminal transferase dUTP nick labelling (TUNEL) staining. Furthermore, U. tomentosa extracts reduced angiogenic markers and reduced the infiltration of T cells into the tumours. Collectively, the results in this study concluded that U. tomentosa has potent anti-cancer activity that significantly inhibited cancer cells in vitro and in vivo.

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