Effects of Dairy Manure Management Practices on E. coli Concentration and Diversity

2016 ◽  
Vol 228 (1) ◽  
Author(s):  
Keya J. Howard ◽  
Emily Martin ◽  
Terry Gentry ◽  
Sam Feagley ◽  
Raghupathy Karthikeyan
Keyword(s):  
Management Practices ◽  
Dairy Manure ◽  
Manure Management ◽  
E Coli

scholarly journals Quantification of antibiotic resistance genes and mobile genetic elements in manure from dairy farms in California

2020 ◽  
Author(s):  
Yi Wang ◽  
Pramod Pandey ◽  
Colleen Chiu ◽  
Richard Jeannotte ◽  
Sundaram Kuppu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Management Practices ◽  
Animal Health ◽  
Antibiotic Resistance Genes ◽  
Dairy Farms ◽  
Mobile Genetic Elements ◽  
Dairy Manure ◽  
Manure Management ◽  
Genetic Elements

Abstract Antibiotic resistance genes (ARGs) are emerging environmental contaminants of concern to both human and animal health. Dairy manure is considered reservoir of ARGs. This study is focused on investigating prevalence of ARGs in California dairy farm manure under current common manure management. A total of 33 manure samples were collected from multiple manure treatment conditions: 1) flushed manure (FM), 2) fresh pile (FP), 3) compost pile (CP), 4) primary lagoon (PL), and 5) secondary lagoon (SL). After DNA extraction, all fecal samples were screened by PCR for the presence of 8 ARGs: four sulfonamide ARGs (sulI, sulII, sulIII, sulA), two tetracycline ARGs (tetW, tetO), two macrolide-lincosamide-streptogramin B (MLSB) ARGs (ermB, ermF). Samples were also screened for two mobile genetic elements (MGEs) (intI1, tnpA), which are responsible for dissemination of ARGs. Quantitative PCR was then used to screen all samples for five ARGs (sulII, tetW, ermF, tnpA and intI1). Prevalence of genes varied among sample types, but all genes were detectable in different manure types. Results showed that liquid-solid separation, piling, and lagoon conditions had limited effects on reducing ARGs and MGEs, and the effect was only found significant on tetW (p = 0.01). Besides, network analysis indicated that sulII was associated with tnpA (p < 0.05), and Psychrobacter and Pseudomonas as opportunistic human pathogens, were potential ARG/MGE hosts (p < 0.05). This research indicated current manure management practices in California dairy farms has limited effects on reducing ARGs and MGEs. Improvement of manure management in dairy farms is thus important to mitigate dissemination of ARGs into the environment.

scholarly journals Quantification of antibiotic resistance genes and mobile genetic in dairy manure

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12408
Author(s):  
Yi Wang ◽  
Pramod Pandey ◽  
Colleen Chiu ◽  
Richard Jeannotte ◽  
Sundaram Kuppu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Management Practices ◽  
Animal Health ◽  
Dairy Farm ◽  
Antibiotic Resistance Genes ◽  
Dairy Farms ◽  
Dairy Manure ◽  
Manure Management ◽  
Human Pathogens

Background Antibiotic resistance genes (ARGs) are considered to be emerging environmental contaminants of concern potentially posing risks to human and animal health, and this research studied the prevalence of antimicrobial resistance in dairy manure. Methods This study is focused on investigating prevalence of ARGs in California dairy farm manure under current common different manure management. A total of 33 manure samples were collected from multiple manure treatment conditions: (1) flushed manure (FM), (2) fresh pile (FP), (3) compost pile (CP), (4) primary lagoon (PL), and (5) secondary lagoon (SL). After DNA extraction, all fecal samples were screened by PCR for the presence of eight ARGs: four sulfonamide ARGs (sulI, sulII, sulIII, sulA), two tetracycline ARGs (tetW, tetO), two macrolide-lincosamide-streptogramin B (MLSB) ARGs (ermB, ermF). Samples were also screened for two mobile genetic elements (MGEs) (intI1, tnpA), which are responsible for dissemination of ARGs. Quantitative PCR was then used to screen all samples for five ARGs (sulII, tetW, ermF, tnpA and intI1). Results Prevalence of genes varied among sample types, but all genes were detectable in different manure types. Results showed that liquid-solid separation, piling, and lagoon conditions had limited effects on reducing ARGs and MGEs, and the effect was only found significant on tetW (p = 0.01). Besides, network analysis indicated that sulII was associated with tnpA (p < 0.05), and Psychrobacter and Pseudomonas as opportunistic human pathogens, were potential ARG/MGE hosts (p < 0.05). This research indicated current different manure management practices in California dairy farms has limited effects on reducing ARGs and MGEs. Improvement of different manure management in dairy farms is thus important to mitigate dissemination of ARGs into the environment.

scholarly journals A Survey of Dairy Manure Management Practices in California

1997 ◽  
Vol 80 (8) ◽  
pp. 1841-1845 ◽  
Author(s):  
D. Morse Meyer ◽  
I. Garnett ◽  
J.C. Guthrie
Keyword(s):  
Management Practices ◽  
Dairy Manure ◽  
Manure Management

Characteristics of solids produced from coal mine drainage and their suitability for phosphorus control in dairy manure management

2020 ◽  
Vol 49 (6) ◽  
pp. 1502-1514
Author(s):  
Robert Hedin ◽  
Benjamin Hedin ◽  
John T. Spargo ◽  
Rachel Brimmer
Keyword(s):  
Coal Mine ◽  
Mine Drainage ◽  
Dairy Manure ◽  
Manure Management ◽  
Coal Mine Drainage ◽  
Phosphorus Control

Comparative efficacy of five different rep-PCR methods to discriminate Escherichia coli populations in aquatic environments

2008 ◽  
Vol 58 (3) ◽  
pp. 537-547 ◽  
Author(s):  
B. R. Mohapatra ◽  
A. Mazumder
Keyword(s):  
Cluster Analysis ◽  
Disease Transmission ◽  
Management Practices ◽  
Gastrointestinal Disease ◽  
Function Analysis ◽  
Average Rate ◽  
Aquatic Environments ◽  
Correct Classification ◽  
Comparative Efficacy ◽  
E Coli

Development of efficient techniques to discriminate the sources of E. coli in aquatic environments is essential to improve the surveillance of fecal pollution indicators, to develop strategies to identify the sources of fecal contamination, and to implement appropriate management practices to minimize gastrointestinal disease transmission. In this study the robustness of five different rep-PCR methods, such as REP-PCR, ERIC-PCR, ERIC2-PCR, BOX-PCR and (GTG)5-PCR were evaluated to discriminate 271 E. coli strains isolated from two watersheds (Lakelse Lake and Okanagan Lake) located in British Columbia, Canada. Cluster analysis of (GTG)5-PCR, BOX-PCR, REP-PCR, ERIC-PCR and ERIC2-PCR profiles of 271 E. coli revealed 43 clusters, 35 clusters, 28 clusters, 23 clusters and 14 clusters, respectively. The discriminant analysis of rep-PCR genomic fingerprints of 271 E. coli isolates yielded an average rate of correct classification (watershed-specific) of 86.8%, 82.3%, 78.4%, 72.6% and 55.8% for (GTG)5-PCR, BOX-PCR, REP-PCR, ERIC-PCR and ERIC2-PCR, respectively. Based on the results of cluster analysis and discriminant function analysis, (GTG)5-PCR was found to be the most robust molecular tool for differentiation of E. coli populations in aquatic environments.

scholarly journals Influence of Tillage and Daily Manure Application on the Survival of Bacterial Pathogens Indicators in Soil and on Radish

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
James A. Entry ◽  
David L. Bjorneberg ◽  
Sheryl Verwey
Keyword(s):  
Bacterial Adhesion ◽  
Raphanus Sativus ◽  
Pathogenic Bacteria ◽  
Rhizosphere Soil ◽  
Bacterial Colonization ◽  
Sampling Period ◽  
Animal Manure ◽  
Dairy Manure ◽  
Control Treatment ◽  
E Coli

We measuredEscherichia coli, andEnterococcussp. numbers in soil and on fresh radish (Raphanus sativusL.) at 1, 7, 14, 28, 54, and 84 days after the addition of high and low amounts of solid dairy manure in combination with chisel tillage to a 20 cm depth (deep) or roller tillage to a 10 cm depth (shallow). When the high or low amount of solid dairy manure was added to the soil,E. colipopulations in soil were higher in the 54 days following manure addition compared to the control treatment. Dairy manure addition increasedEnterococcussp. in soils compared to the control treatment for the entire 84 days sampling period. At harvest, which was 84 days after application, we did not detectE. coliin radish in rhizosphere soil or on radish roots. Addition of solid dairy manure increasedEnterococcussp. numbers in radish rhizosphere soil and on radish roots. We suggest that fresh animal manure be applied to soil at least 120 days prior to planting to allow die-off of human pathogenic bacteria and reduce the incidence of bacterial adhesion on or bacterial colonization of ready to eat vegetables.

Combining perennial grass-legume forages and liquid dairy manure contributes to nitrogen accumulation in a clayey soil

2021 ◽  
Author(s):  
Emmanuelle D’Amours ◽  
Martin H. Chantigny ◽  
Anne Vanasse ◽  
Émilie Maillard ◽  
Jean Lafond ◽  
...  
Keyword(s):  
Management Practices ◽  
Clayey Soil ◽  
Perennial Grass ◽  
Dairy Manure ◽  
Nitrogen Accumulation ◽  
Silty Clay ◽  
Soil N ◽  
Mineral Fertilization ◽  
Humid Climate ◽  
Tillage Practices

Repeated applications of liquid dairy manure (LDM) and perennial crops generally favor nitrogen (N) stocks in soils, but in ways that may differ with soil type and other management practices. The objective of this study was to assess the long-term (21 yr) changes in soil N stocks (0–50 cm) of a silty clay soil, in a cool humid climate, in response to mineral fertilization (MIN) or LDM, combined with two tillage practices (chisel plow [CP], or moldboard plow [MP]), and two crop rotations (cereal monoculture [monoculture] or cereal-perennial forage rotation [forage-based rotation].) The forage-based rotation favoured a greater accumulation of N in the first 20 cm of soil (+50 kg N ha-1 y-1) when compared to the monoculture. Tillage practices did not impact N stock in the whole soil profile, but influenced its vertical distribution, with greater accumulation at the surface with CP, and at depth with MP. Annual input of LDM increased N stocks at the surface (0–20 cm) compared to MIN, especially when combined with the forage-based rotation. After 21 yr, soil N stocks (0-50 cm) with LDM were 32% (+2 t N ha-1) higher in the forage-based rotation than in the monoculture, suggesting better retention and more efficient use of manure-N with perennial forages than cereals. Comparisons between the N mass balance computed for each cropping system and the changes in soil N stocks indicated that accumulation of N under the forage-based rotation was largely due to symbiotic fixation by legumes in the forage mixture.

Differential Survival of Generic E. coli and Listeria spp. in Northeastern U.S. Soils Amended with Dairy Manure Compost, Poultry Litter Compost, and Heat-Treated Poultry Pellets and Fate in Raw Edible Radish Crops

2021 ◽  
Author(s):  
Marie Limoges ◽  
Deborah A. Neher ◽  
Thomas R. Weicht ◽  
Patricia D. Millner ◽  
Manan Sharma ◽  
...  
Keyword(s):  
Fresh Produce ◽  
Poultry Litter ◽  
Dairy Manure ◽  
Animal Origin ◽  
Most Probable Number ◽  
Post Inoculation ◽  
Probable Number ◽  
E Coli ◽  
Manure Compost ◽  
Heat Treated

Composted or heat-treated Biological Soil Amendments of Animal Origin (BSAAO) can be added to soils to provide nutrients for fresh produce. These products lower the risk of pathogen contamination of fresh produce when compared with use of untreated BSAAO; however, meteorological conditions, geographic location, and soil properties can influence the presence of pathogenic bacteria, or their indicators (e.g., generic E. coli) and allow potential for produce contamination. Replicated field plots of loamy or sandy soils were tilled and amended with dairy manure compost (DMC), poultry litter compost (PLC), or no compost (NoC) over two different field seasons, and non-composted heat-treated poultry pellets (HTPP) during the second field season. Plots were inoculated with a three-strain cocktail of rifampicin-resistant E. coli (rE.coli) at levels of 8.7 log CFU/m2. Direct plating and most probable number (MPN) methods measured the persistence of rE.coli and Listeria spp. in plots through 104 days post-inoculation. Greater survival of rE. coli was observed in PLC plots in comparison to DMC plots and NoC plots during year 1 (P &lt; 0.05). Similar trends were observed for year 2, where rE. coli survival was also greater in HTPP amended plots (P &lt; 0.05). Survival of rE. coli was dependent on soil type, where water potential and temperature were significant covariables. Listeria spp. were found in NoC plots, but not in plots amended with HTPP, PLC or DMC. Radish data demonstrate that PLC treatment promoted the greatest level of rE.coli translocation when compared to DMC and NoC treatments (P  &lt; 0.05). These results are consistent with findings from studies conducted in other regions of the US and informs Northeast produce growers that composted and non-composted poultry-based BSAAO supports greater survival of rE. coli in field soils. This result has the potential to impact the food safety risk of edible produce grown in BSAAO amended soils as a result of pathogen contamination.

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