Use of alginate and cryo-protective sugars to improve the viability of lactic acid bacteria after freezing and freeze-drying

2005 ◽  
Vol 21 (5) ◽  
pp. 739-746 ◽  
Author(s):  
B. De Giulio ◽  
P. Orlando ◽  
G. Barba ◽  
R. Coppola ◽  
M. De Rosa ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

Role of Cryoprotectants on the Viability and Functional Properties of Probiotic Lactic Acid Bacteria during Freeze Drying

2009 ◽  
Vol 23 (3) ◽  
pp. 243-265 ◽  
Author(s):  
Kanchi Bhasker Praveen Kumar Reddy ◽  
Sharda Prasad Awasthi ◽  
Arenahalli Ningegowda Madhu ◽  
Siddalingaiya Gurudutt Prapulla
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Functional Properties ◽  
Freeze Drying ◽  
Probiotic Lactic Acid Bacteria

The Freeze-Drying of Lactic Acid Bacteria. A Review

1991 ◽  
Vol 24 (3-4) ◽  
pp. 118-128 ◽  
Author(s):  
C.P. Champagne ◽  
N. Gardner ◽  
E. Brochu ◽  
Y. Beaulieu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

scholarly journals UTILIZATION PROBIOTIC LACTIC ACID BACTERIA FISH FROM WASTE FAECES ON LDL LEVEL BLOOD BROILER CHICKENS STRAIN LOHMANN

2017 ◽  
Vol 5 (1) ◽  
pp. 51 ◽  
Author(s):  
Astuti Astuti ◽  
Siti Umniyati ◽  
Anna Rakhmawati ◽  
Evy Yulianti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Count ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Freeze Drying ◽  
Animal Husbandry ◽  
Control Treatment ◽  
Probiotic Lactic Acid Bacteria ◽  
Chicken Blood

Abstract This study aims to determine the effect of lactic acid bacteria supplement from sewge force feeding fish to the LDL content of broiler chicken blood. The research samples are 40 of 1 day old broiler chicken from PT. Multi Breeder Adiram, which were divided into 4 treatments, were taken randomly and maintined for 42 days. Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 107CFU / ml. , IV treatment BAL cell count was 108 CFU /ml. The results showed that there is no significant effect of BAL isolate addition on the LDL level of the broiler chicken blood. Keywords: lactic acid bacteria, LDL, freeze drying

THE EFFECT OF FREEZE DRYING ON THE ACTIVITY OF LACTIC ACID BACTERIA

2021 ◽  
Vol 23 (2) ◽  
pp. 50-52
Author(s):  
A.F. Novikova ◽  
◽  
Zh.A. Grigoryeva ◽  
P.Yu. Kotlyar ◽  
◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

scholarly journals Influence of freezing temperatures prior to freeze-drying on viability of yeasts and lactic acid bacteria isolated from wine

2017 ◽  
Vol 122 (6) ◽  
pp. 1603-1614 ◽  
Author(s):  
L. Polo ◽  
R. Mañes-Lázaro ◽  
I. Olmeda ◽  
L.E. Cruz-Pio ◽  
Á. Medina ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying ◽  
Freezing Temperatures

Freeze-Drying of Lactic Acid Bacteria

2014 ◽  
pp. 477-488 ◽  
Author(s):  
Fernanda Fonseca ◽  
Stéphanie Cenard ◽  
Stéphanie Passot
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

scholarly journals Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

2016 ◽  
Vol 11 (3) ◽  
pp. 573 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Rajib Majumder ◽  
Irfan A. Rather ◽  
Kangmin Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Trichloroacetic Acid ◽  
Freeze Drying ◽  
Precipitation Method ◽  
Isolation And Purification ◽  
Video Clips ◽  
Response System ◽  
Ethanol Precipitation ◽  
Scanning Electron

<p>Lactic acid bacteria are classified ‘Generally Recognized As Safe’ (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/PGDyHPEk5jY">Part 1</a>:   7 min 42 sec</p><p><a href="https://youtube.com/v/G8ZMZ4z1DMs">Part 2</a>:   3 min 36 sec</p>

Comparative Survival Rates of Lactic Acid Bacteria Isolated from Blood, Following Spray-drying and Freeze-drying

2006 ◽  
Vol 12 (1) ◽  
pp. 77-84 ◽  
Author(s):  
L. M. Zamora ◽  
C. Carretero ◽  
D. Parés
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying ◽  
Freeze Drying ◽  
Survival Rates ◽  
Storage Period ◽  
Cellular Damage ◽  
Viable Cells ◽  
Porcine Blood ◽  
Freeze Dried

The effect of two dehydration technologies, spray-drying and freeze-drying, on the viability of 12 lactic acid bacteria (LAB) were compared. All LAB cultures had been previously isolated from porcine blood and were candidates to be used as biopreservatives in order to maintain the quality of porcine blood until further processing to obtain added-value blood derivatives is carried out. The residual viability and the reductions in microbial counts in dried LAB samples at 20 °C and 5 °C during 60-day storage were determined. Cellular damage due to freeze-drying was observed immediately after drying whereas cellular damage due to spray-drying did not become evident until the subsequent phase of storage. For most of the strains, the faster decrease in viability of spray-dried as compared to freeze-dried cultures was compensated by the higher percentage of viable cells obtained after dehydration, leading to comparable survival rates at the end of the storage period. Dehydration resulted in a good alternative to freezing at 80 °C for preservation purposes. Spray-drying has been shown to be as suitable as freeze-drying for preserving LAB strains during a 2-month storage period. Results suggest the possibility of achieving a good formulation system for the LAB strains with a high number of viable cells to be used for the industrial development of bioprotective cultures.

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