Spray Drying, Freeze Drying, or Freezing of Three Different Lactic Acid Bacteria Species

1997 ◽  
Vol 62 (3) ◽  
pp. 576-578 ◽  
Author(s):  
BRIAN C. S. TO ◽  
MARK R. ETZEL
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying ◽  
Freeze Drying

Comparative Survival Rates of Lactic Acid Bacteria Isolated from Blood, Following Spray-drying and Freeze-drying

2006 ◽  
Vol 12 (1) ◽  
pp. 77-84 ◽  
Author(s):  
L. M. Zamora ◽  
C. Carretero ◽  
D. Parés
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying ◽  
Freeze Drying ◽  
Survival Rates ◽  
Storage Period ◽  
Cellular Damage ◽  
Viable Cells ◽  
Porcine Blood ◽  
Freeze Dried

The effect of two dehydration technologies, spray-drying and freeze-drying, on the viability of 12 lactic acid bacteria (LAB) were compared. All LAB cultures had been previously isolated from porcine blood and were candidates to be used as biopreservatives in order to maintain the quality of porcine blood until further processing to obtain added-value blood derivatives is carried out. The residual viability and the reductions in microbial counts in dried LAB samples at 20 °C and 5 °C during 60-day storage were determined. Cellular damage due to freeze-drying was observed immediately after drying whereas cellular damage due to spray-drying did not become evident until the subsequent phase of storage. For most of the strains, the faster decrease in viability of spray-dried as compared to freeze-dried cultures was compensated by the higher percentage of viable cells obtained after dehydration, leading to comparable survival rates at the end of the storage period. Dehydration resulted in a good alternative to freezing at 80 °C for preservation purposes. Spray-drying has been shown to be as suitable as freeze-drying for preserving LAB strains during a 2-month storage period. Results suggest the possibility of achieving a good formulation system for the LAB strains with a high number of viable cells to be used for the industrial development of bioprotective cultures.

Spray-Drying of Bacteriocin-Producing Lactic Acid Bacteria

1999 ◽  
Vol 62 (7) ◽  
pp. 773-777 ◽  
Author(s):  
GIANLUIGI MAURIELLO ◽  
MARIA APONTE ◽  
ROSAMARIA ANDOLFI ◽  
GIANCARLO MOSCHETTI ◽  
FRANCESCO VILLANI
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Survival ◽  
Spray Drying ◽  
Antagonistic Activity ◽  
Cellular Damage ◽  
Bacteriocin Activity ◽  
Lactobacillus Curvatus ◽  
Significant Difference ◽  
Inhibitory Spectrum

Cell survival, cellular damage, and antagonistic activity were investigated after spray-drying of four bacteriocin-producing strains of lactic acid bacteria: Lactococcus lactis subsp. lactis 140, isolated from natural whey culture and producing a narrow-inhibitory spectrum bacteriocin); L. lactis subsp. lactis G35, isolated from pizza dough and producing nisin; Lactobacillus curvatus 32Y and Lactobacillus sp. 8Z, isolated from dry sausages. Trials were performed with bacteria suspended in skimmed milk or directly grown in whey. Three air temperatures at the inlet of the drier (160, 180, and 200°C) and three flow rates (10, 13, and 17 ml/min) were assayed. Cell viability and bacteriocin activity of the dried materials were determined immediately after the process and after 5, 15, 30, and 60 days of storage at 4°C. There was no significant difference between the two feeding suspensions in cell survival, always decreasing with the increase of inlet-air temperature. No loss of bacteriocin activity was detected in reconstituted powders, nor was any loss of ability to produce bacteriocin found after drying. Investigations of sensitivity to NaCl revealed only temporary damage to dried bacteria. During storage for 2 months at 4°C, all samples, but mainly the lactococcal strains, displayed a gradual decrease in cell survival. Bacteriocin activity remained at the same level, allowing powders to be considered as effective biopreservatives.

Role of Cryoprotectants on the Viability and Functional Properties of Probiotic Lactic Acid Bacteria during Freeze Drying

2009 ◽  
Vol 23 (3) ◽  
pp. 243-265 ◽  
Author(s):  
Kanchi Bhasker Praveen Kumar Reddy ◽  
Sharda Prasad Awasthi ◽  
Arenahalli Ningegowda Madhu ◽  
Siddalingaiya Gurudutt Prapulla
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Functional Properties ◽  
Freeze Drying ◽  
Probiotic Lactic Acid Bacteria

The Freeze-Drying of Lactic Acid Bacteria. A Review

1991 ◽  
Vol 24 (3-4) ◽  
pp. 118-128 ◽  
Author(s):  
C.P. Champagne ◽  
N. Gardner ◽  
E. Brochu ◽  
Y. Beaulieu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

scholarly journals The Viability of Lactobacillus plantarum From Intestines of Laying Ducks Encapsulated With Arabic Gum and Skimmed Milk

2021 ◽  
Vol 16 (3) ◽  
pp. 190-199
Author(s):  
Badat Muwakhid ◽  
Anik Maunatin ◽  
Anif Mukaromah Wati
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying ◽  
Skim Milk ◽  
Plate Count ◽  
Resistance Testing ◽  
Drying Process ◽  
Arabic Gum ◽  
Before And After ◽  
Skimmed Milk

The aim of this study was to evaluate the effect of the types of encapsulation materials, that is skimmed milk and Arabic gum on two probiotics Lactic Acid Bacteria (LAB), including L. plantarum DJ2 and L. plantarum DJ3. The methods of this study were separated into two stages that is probiotic resistance testing during the spray drying process and the viability of LAB after spray drying during storage of probiotic powder for one month at 4oC. Changes in the viability of LAB probiotics before and after the drying process using spray drying were determined by the total plate count. The viability of lactic acid bacteria (LAB) was observed every week for one month of storage at 4oC. The results showed that different encapsulation materials had significant different (P ≤ 0.05) on changes in resistance of probiotics powder during spray drying process. The use of Arabic gum could increase the resistance of probiotics during the spray drying process, meanwhile, the use of skimmed milk was better to be able to maintain the viability of dry probiotic powder both on L.plantarum DJ2 and L.plantarum DJ3 during storage. After four weeks of storage, there was mold and yeast in the skimmed milk and Arabic gum materials. In conclusion, both Arabic gum and skim milk could be used for encapsulation where storage of dry probiotic products is recommended during one month at 4 oC.

A novel insight to screen the optimal spray-drying protectants and parameters for manufacturing lactic acid bacteria preparations

2019 ◽  
Vol 38 (14) ◽  
pp. 1843-1856 ◽  
Author(s):  
Zhuo Zhang ◽  
Sen Peng ◽  
Xiaoqi Sun ◽  
Yu Jie ◽  
Hongfei Zhao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying

EXPERIMENTAL VACUUM SPRAY DRYING OF PROBIOTIC FOODS INCLUDED WITH LACTIC ACID BACTERIA

2009 ◽  
Vol 33 (6) ◽  
pp. 714-726 ◽  
Author(s):  
YUTAKA KITAMURA ◽  
HIROYUKI ITOH ◽  
HIROSHI ECHIZEN ◽  
TAKAAKI SATAKE
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying

Use of alginate and cryo-protective sugars to improve the viability of lactic acid bacteria after freezing and freeze-drying

2005 ◽  
Vol 21 (5) ◽  
pp. 739-746 ◽  
Author(s):  
B. De Giulio ◽  
P. Orlando ◽  
G. Barba ◽  
R. Coppola ◽  
M. De Rosa ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying

Survival of Lactic Acid Bacteria during Spray Drying of Plain Yogurt

1990 ◽  
Vol 55 (4) ◽  
pp. 1008-1010 ◽  
Author(s):  
SUK SHIN KIM ◽  
SANTI R. BHOWMIK
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying

scholarly journals UTILIZATION PROBIOTIC LACTIC ACID BACTERIA FISH FROM WASTE FAECES ON LDL LEVEL BLOOD BROILER CHICKENS STRAIN LOHMANN

2017 ◽  
Vol 5 (1) ◽  
pp. 51 ◽  
Author(s):  
Astuti Astuti ◽  
Siti Umniyati ◽  
Anna Rakhmawati ◽  
Evy Yulianti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Count ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Freeze Drying ◽  
Animal Husbandry ◽  
Control Treatment ◽  
Probiotic Lactic Acid Bacteria ◽  
Chicken Blood

Abstract This study aims to determine the effect of lactic acid bacteria supplement from sewge force feeding fish to the LDL content of broiler chicken blood. The research samples are 40 of 1 day old broiler chicken from PT. Multi Breeder Adiram, which were divided into 4 treatments, were taken randomly and maintined for 42 days. Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 107CFU / ml. , IV treatment BAL cell count was 108 CFU /ml. The results showed that there is no significant effect of BAL isolate addition on the LDL level of the broiler chicken blood. Keywords: lactic acid bacteria, LDL, freeze drying

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