Changes in physico-chemical characteristics and viable bacterial communities during fermentation of alfalfa silages inoculated with Lactobacillus plantarum

Author(s):  
Chenxia Cao ◽  
Weichen Bao ◽  
Weicheng Li ◽  
Feiyan Zhao ◽  
Lai-yu Kwok ◽  
...  
2020 ◽  
Author(s):  
Juntao Zhang ◽  
Xiying Huang ◽  
Menggen Ma ◽  
Quanju Xiang ◽  
Ke Zhao ◽  
...  

Abstract Background:Silage fermentation, a sustainable way to use vegetable waste resources, is a complex process driven by a variety of microorganisms. We used lettuce waste as the raw material for silage, analyzed changes in the physico-chemical characteristics and bacterial community composition of silage during a 60 day fermentation, identified differentially abundant taxa, predicted the functional profiles of bacterial communities, and determined the associated effects on the quality of silage. Results: The biggest changes occurred in the early stage of silage fermentation. Changes in the physico-chemical characteristics included a decrease in pH and increases in ammonia nitrogen to total nitrogen ratio and lactic acid content. The numbers of lactic acid bacteria increased and those of molds, yeasts and aerobic bacteria decreased. The bacterial communities and their predicted functions on day 0 were clearly different from those on day 7 to day 60. The relative abundances of phylum Firmicutes and genus Lactobacillus increased. Nitrite ammonification and nitrate ammonification were more prevalent after day 0. The differences in the predicted functions were associated with differences in pH and amino acid, protein, carbohydrate, NH3-N, ether extract and crude ash contents. Conclusion: Firmicutes and Lactobacillus were the dominant taxa during vegetable waste silage fermentation. The microbial communities and the predicted functions changed in different stages of silage fermentation, and the changes were accompanied with changes in the physico-chemical characteristics, especially with a decrease in pH and increases in ammonia nitrogen to total nitrogen ratio and lactic acid content.


2017 ◽  
Vol 41 (1) ◽  
pp. 61 ◽  
Author(s):  
Ahmad Sofyan ◽  
Yantyati Widyastuti ◽  
Ristianto Utomo ◽  
Lies Mira Yusiati

This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical characteristics and palatability of king grass (Pennisetum hybrid) silage. The experiment was arranged on the factorial randomized design (3x3) consisting of the inoculants treatments (control, Lp, Lp+Sc) and the addition level of rice bran (0, 5 and 10%). The measured variables were physico-chemical characteristics i.e. colour, odour, pH, lactic acid, Fleigh points, and palatability of silage. Inoculation of Lp and Lp+Sc improved silage odour and reduced fungal contamination. Silage was treated by Lp+Sc and rice bran (5-10%) showed reduction of pH and an increase of lactic acid and Fleigh points. However, interaction between inoculants and rice bran treatment was not significance. Either inoculation or addition of rice bran tended to enhance the palatability of silage in cattle. It concluded that the addition of inoculants L. plantarum and S. cerevisiae with/without addition of 5-10% rice bran could improve the physico-chemical characteristics of silage and its palatability to ruminant.


Author(s):  
Quanju Xiang ◽  
Juntao Zhang ◽  
Xiying Huang ◽  
Menggen Ma ◽  
Ke Zhao ◽  
...  

Silage fermentation, a sustainable way to use vegetable waste resources, is a complex process driven by a variety of microorganisms. We used lettuce waste as the main raw material for silage, analyzed changes in the physico-chemical characteristics and bacterial community composition of silage over a 60-day fermentation, identified differentially abundant taxa, predicted the functional profiles of bacterial communities, and determined the associated effects on the quality of silage. The biggest changes occurred in the early stage of silage fermentation. Changes in the physico-chemical characteristics included a decrease in pH and increases in ammonia nitrogen to total nitrogen ratio and lactic acid content. The numbers of lactic acid bacteria (LAB) increased and molds, yeasts and aerobic bacteria decreased. The bacterial communities and their predicted functions on day 0 were clearly different from those on day 7 to day 60. The relative abundances of phylum Firmicutes and genus Lactobacillus increased. Nitrite ammonification and nitrate ammonification were more prevalent after day 0. The differences in the predicted functions were associated with differences in pH and amino acid, protein, carbohydrate, NH3-N, ether extract and crude ash contents.


LWT ◽  
2018 ◽  
Vol 92 ◽  
pp. 195-203 ◽  
Author(s):  
Sirine Ben Slima ◽  
Naourez Ktari ◽  
Mehdi Triki ◽  
Imen Trabelsi ◽  
Asehraou Abdeslam ◽  
...  

2019 ◽  
Vol 70 (7) ◽  
pp. 2534-2537
Author(s):  
Gladiola Tantaru ◽  
Mihai Apostu ◽  
Antonia Poiata ◽  
Mihai Nichifor ◽  
Nela Bibire ◽  
...  

The paper presents the synthesis of a new complex combination of a Bis-Schiff base with Mn(II) ions with great potential for antimicrobial and anti-inflammatory activity. A new complex of the Salen-type ligand, 1-ethyl-salicylidene-bis-ethylene diamine was synthetized using Mn(II) ions. The chemical structure was confirmed through 1H-NMR and IR spectroscopy. The antimicrobial activities of the Bis-Schiff base and its complex were tested in comparison with Ampicillin, Chloramphenicol, Tetracycline, Ofloxacin and Nystatin. Those compounds were found to be active against Gram-positive or Gram-negative bacteria, and had an anti-inflammatory effect comparable to that of Indomethacin.


2020 ◽  
Vol 16 (5) ◽  
pp. 685-707 ◽  
Author(s):  
Amna Batool ◽  
Farid Menaa ◽  
Bushra Uzair ◽  
Barkat Ali Khan ◽  
Bouzid Menaa

: The pace at which nanotheranostic technology for human disease is evolving has accelerated exponentially over the past five years. Nanotechnology is committed to utilizing the intrinsic properties of materials and structures at submicroscopic-scale measures. Indeed, there is generally a profound influence of reducing physical dimensions of particulates and devices on their physico-chemical characteristics, biological properties, and performance. The exploration of nature’s components to work effectively as nanoscaffolds or nanodevices represents a tremendous and growing interest in medicine for various applications (e.g., biosensing, tunable control and targeted drug release, tissue engineering). Several nanotheranostic approaches (i.e., diagnostic plus therapeutic using nanoscale) conferring unique features are constantly progressing and overcoming all the limitations of conventional medicines including specificity, efficacy, solubility, sensitivity, biodegradability, biocompatibility, stability, interactions at subcellular levels. : This review introduces two major aspects of nanotechnology as an innovative and challenging theranostic strategy or solution: (i) the most intriguing (bare and functionalized) nanomaterials with their respective advantages and drawbacks; (ii) the current and promising multifunctional “smart” nanodevices.


Sign in / Sign up

Export Citation Format

Share Document