IMPROVING PHYSICO-CHEMICAL CHARACTERISTIC AND PALATABILITY OF KING GRASS (Pennisetum hybrid) SILAGE BY INOCULATION OF Lactobacillus plantarum - Saccharomyces cerevisiae CONSORTIA AND ADDITION OF RICE BRAN

This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical characteristics and palatability of king grass (Pennisetum hybrid) silage. The experiment was arranged on the factorial randomized design (3x3) consisting of the inoculants treatments (control, Lp, Lp+Sc) and the addition level of rice bran (0, 5 and 10%). The measured variables were physico-chemical characteristics i.e. colour, odour, pH, lactic acid, Fleigh points, and palatability of silage. Inoculation of Lp and Lp+Sc improved silage odour and reduced fungal contamination. Silage was treated by Lp+Sc and rice bran (5-10%) showed reduction of pH and an increase of lactic acid and Fleigh points. However, interaction between inoculants and rice bran treatment was not significance. Either inoculation or addition of rice bran tended to enhance the palatability of silage in cattle. It concluded that the addition of inoculants L. plantarum and S. cerevisiae with/without addition of 5-10% rice bran could improve the physico-chemical characteristics of silage and its palatability to ruminant.

Download Full-text

Reduction in the ammonia content of salmon shark meat by a fermented rice bran suspension with the Satoumi-sourced yeast Saccharomyces cerevisiae Misaki-1 and lactic acid bacteria Lactobacillus plantarum Sanriku-SU8

LWT ◽

10.1016/j.lwt.2015.12.022 ◽

2016 ◽

Vol 68 ◽

pp. 244-250 ◽

Cited By ~ 10

Author(s):

Takashi Kuda ◽

Saya Kondo ◽

Yoko Usami ◽

Shoichiro Ishizaki ◽

Hajime Takahashi ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Rice Bran ◽

Yeast Saccharomyces Cerevisiae ◽

Ammonia Content

Download Full-text

Populasi Bakteri dan Keberadaan Bakteri gram (+) dan (-) dalam Pellet Calf Starter yang Ditambah Limbah Kubis Fermentasi

Jurnal Ilmu Nutrisi dan Teknologi Pakan ◽

10.29244/jintp.16.3.65-68 ◽

2019 ◽

Vol 16 (3) ◽

pp. 65

Author(s):

Sri Mukodiningsih ◽

B.. Sulistiyanto ◽

SS Sholikhah

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Rice Bran ◽

Descriptive Analysis ◽

Average Score ◽

Randomized Design ◽

Completely Randomized Design ◽

Addition Level ◽

Level 2 ◽

Total Bacteria

The aim of the experiment was to evaluate total bacteria population and the existence of positive and negative gram bacteria of pelletcalf starter which added by lactic acid bacteria from fermented cabbage waste. The materials in this research were corn, rice bran, soybean meal, mineral mix, molasses, fermented cabbage wasted. The research was conducted by Completely Randomized Design with 4 treatments and 5 replications. The treatments were T0 (100% calf starter), T1 (2% of fermented cabbage waste + 100% calf starter), T2 (4% of fermented cabbage waste + 100% calf starter) and T3 (6% of fermented cabbage waste + 100% calf starter). The Variables observed were bacteria population and gram bacteria. Data of bacteria population were evaluated by descriptive analysis, while data of gram bacteria existence were statistically analyses by variance analysis, if there were significantly affect continued by Duncan test. The results showed that the highest bacteria population was on the treatment with addition level 2% of fermented cabbage waste and the lowest bacteria population was on addition level 6%. Adding fermented cabbage waste did not significantly effect to the existence of gram bacteria. The average score of gram bacteria existence on added fermented cabbage waste from level 0%, 2%, 4%, and 6% were respectively 1.99; 2.10; 2.29 and 2.29. Positive gram bacteria extended increased with increased fermented cabbage waste. The conclusion was the pellet calf starter which added with lactic acid bacteria produced bacteria and there was positive gram bacteria, and suppress negative bacteria.

Download Full-text

The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2013.2288 ◽

2013 ◽

pp. 92

Author(s):

Ida N Jamal ◽

Reiny A Tumbol ◽

Remy E.P Mangindaan

Keyword(s):

Saccharomyces Cerevisiae ◽

Immune System ◽

Aeromonas Hydrophila ◽

Phagocytic Activity ◽

Baker’S Yeast ◽

Baker's Yeast ◽

Yeast Saccharomyces Cerevisiae ◽

Randomized Design ◽

The Difference ◽

Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of 10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

Download Full-text

PENGARUH KONSENTRASI STARTER BAKTERI Lactobacillus plantarum TERHADAP BEBERAPA KOMPONEN MUTU TEPUNG PORANG (Amorphophallus oncophyllus)

Pro Food ◽

10.29303/profood.v6i2.136 ◽

2021 ◽

Vol 6 (2) ◽

pp. 673

Author(s):

Ade Irma Juliana ◽

Moegiratul Amaro ◽

Nazaruddin Nazaruddin

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Protein Content ◽

Lactobacillus Plantarum ◽

Analysis Of Variance ◽

Ph Value ◽

Significance Level ◽

Randomized Design ◽

Significant Difference ◽

Polynomial Orthogonal

ABSTRACT This study aims to determine the effect of Lactobacillus plantarum bacterial starter concentration on some quality of the porang flour. This study used experimental design one-factor Completely Randomized Design (CRD) consisted of BAL concentration (KB) of Lactobacillus plantarum with 6 treatments which is 0%, 5%, 10%, 15%, 20% and 25%. Each of treatment were repeated three times to obtain 18 unit samples. Data from observations were analyzed using analysis of variance (Analysis of Variance) at 5% significance level using Co-stat software. If there are significant differences, a further Polynomial Orthogonal and Honestly Significant Difference (BNJ) test is performed at a level of 5%. The parameters observed included pH value, protein content, water content, yield, total lactic acid bacteria, organoleptic parameters of color and aroma (hedonic and scoring). The results showed that the concentration of Lactobacillus plantarum bacterial 20% was the best treatment in producing porang flour, pH value 5.72, protein content 6.49%, yield 9.33%, total lactic acid bacteria 6.66 log CFU / g and color rather brown and slightly acidic aroma and somewhat preferred by panelists. Keywords: Porang flour, starter concentration, Lactobacillus plantarum ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi starter bakteri Lactobacillus plantarum terhadap beberapa komponen mutu tepung porang. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yakni konsentrasi BAL (KB) jenis Lactobacillus plantarum dengan 6 perlakuan yaitu 0%, 5%, 10%, 15%, 20% dan 25%. Masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Data hasil pengamatan dianalisis menggunakan analisis keragaman (Analysis of Variance) pada taraf nyata 5% menggunakan software Co-stat. Apabila terdaapat beda nyata, dilakukan uji lanjut Polynomial Orthogonal dan Beda Nyata Jujur (BNJ) pada taraf 5%. Adapun parameter yang diamati meliputi nilai pH, kadar protein, kadar air, rendemen, total bakteri asam laktat, parameter organoleptik warna dan aroma (hedonik dan scoring). Hasil penelitian menunjukkan bahwa konsentrasi strater bakteri Lactobacillus plantarum 20% merupakan perlakuan terbaik dalam menghasilkan tepung porang nilai pH 5,72, kadar protein 6,49%, rendemen 9,33%, total bakteri asam laktat 6,66 log CFU/g serta warna agak coklat dan aroma agak asam serta agak disukai panelis. Kata Kunci: Tepung porang, konsentrasi starter, Lactobacillus plantarum

Download Full-text

Karakteristik Tepung Ampas Tahu yang Difermentasi dengan Lactobacillus plantarum terhadap kandungan Daidzein, Glycitein, dan Genistein

BUANA SAINS ◽

10.33366/bs.v20i1.1931 ◽

2020 ◽

Vol 20 (1) ◽

pp. 49-56

Author(s):

Eka Fitasari ◽

Budi Santosa

Keyword(s):

Lactic Acid ◽

Lactobacillus Plantarum ◽

Fermentation Process ◽

Animal Feed ◽

High Energy ◽

Microbial Culture ◽

Gram Positive Bacteria ◽

Randomized Design ◽

Completely Randomized Design

Animal feed should ideally be inexpensive and not competing with humans. Ampas know is a waste of the manufacture of tofu that contains protein and high energy. Its use has problems due to the existence of antinutrient and cannot be kept long. Lactobacillus Plantarum is a lactic acid (Gram-positive) bacteria used in the fermentation process to improve the quality of feed. The goal of fermentation is to remove the anti-nutrients that exist in the tofu pulp and increase the content of Isoflavone (Daidzin, Glycitein, and Genistein) and Isoflavone is a source of antioxidants important to growth. The study uses a Completely Randomized Design with 5 treatment of microbial culture concentrations of 1%, 5%, 10%, 15%, and 20% (V/w), respectively repeated 3 times. The result of analyzes in the pulp of tofu fermentation suggests that fermentation by Lactobacillus Plantarum can increase the content of Daidzein, Glycitein, and genistein with the highest value resulting from the use of L. Plantarum 20% respectively 191.21 + 24.9 μg/g, 85.96 μg/g, and 120.82 μg/g. It can be concluded that the higher the use of the concentration of L. Plantarum in tofu pulp can increase the levels of isoflavone, so it is advisable to conduct research with the concentration of L. Plantarum higher in tofu pulp

Download Full-text

The Effect of Using Biological Treatment on Microbial Growth during the Malting of Sorghum

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i1130405 ◽

2021 ◽

pp. 105-114

Author(s):

Oluwatosin Charles Ayodeji ◽

Afolabi Folake Titilayo ◽

Abdulkadir Musliu ◽

Fasiku Oluwafemi

Keyword(s):

Saccharomyces Cerevisiae ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Starter Cultures ◽

Polyphenol Content ◽

Sorghum Malt ◽

Combined Cultures ◽

Sorghum Grain ◽

Phytate Content

Malting is an important industrial product with a huge market outlet. Sorghum grain carries a numerous and variable, microbial population that mainly consists of bacteria, yeasts, and filamentous fungi. Sorghum malt is heavily reliant on chemical control of moulds and coliforms. This research aimed at investigating ways of improving malt quality and safety, using starter cultures of lactic acid bacteria and yeast, during the steeping stage of malting. All the steep treatments contained a sizeable population of moulds, greater than 4logcfu/mL, at 0hrs of steeping. A 3Log decrease was recorded in the steep treatment containing only single culture of Lactobacillus plantarum All the steeping treatments achieved varying levels of anti-nutrient reduction. The Lactobacillus plantarum CLB8 steep reduced the phytate level by as much as 47% when compared to the phytate level in sorghum grain. The combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiaeCYT1 reduced the phytate content by as much as 40% when compared to the sorghum grain without treatment. When compared to the control steep, the Lactobacillus plantarum CLB8 steep improved the anti-nutrient degradation by 31%. The combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiae CYT1 reduced the phytate content by as much as 23% when compared with the control steep. The polyphenol content was reduced by about 46% in the Lactobacillus plantarum CLB8 steep and 29% in the combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiae CYT1 steep when compared to the polyphenol content in the whole sorghum grain. Only the Lactobacillus plantarum CLB8 steep had better polyphenol reduction than the control with a 9.6% reduction more than the control. It was concluded that lactic acid bacteria can be apply as a biological control organism in malting of grains.

Download Full-text

Induction of the synthesis of an additional family of long-chain dolichols in the yeast Saccharomyces cerevisiae. Effect of starvation and ageing.

Acta Biochimica Polonica ◽

10.18388/abp.2002_3786 ◽

2002 ◽

Vol 49 (3) ◽

pp. 781-787 ◽

Cited By ~ 6

Author(s):

Anna Szkopinska ◽

Ewa Swiezewska ◽

Joanna Rytka

Keyword(s):

Saccharomyces Cerevisiae ◽

Stationary Phase ◽

Yeast Cells ◽

Logarithmic Phase ◽

Cellular Membranes ◽

Yeast Saccharomyces Cerevisiae ◽

Chemical Changes ◽

Physico Chemical ◽

Long Chain

The yeast Saccharomyces cerevisiae strain W303 synthesizes in the early logarithmic phase of growth dolichols of 14-18 isoprene residues. The analysis of the polyisoprenoids present in the stationary phase revealed an additional family which proved to be also dolichols but of 19-24 isoprene residues, constituting 39% of the total dolichols. The transfer of early logarithmic phase cells to a starvation medium lacking glucose or nitrogen resulted in the synthesis of the longer chain dolichols. The additional family of dolichols represented 13.8% and 10.3% of total dolichols in the glucose and nitrogen deficient media, respectively. The level of dolichols in yeast cells increased with the age of the cultures. Since both families of dolichols are present in stationary phase cells we postulate that the longer chain dolichols may be responsible for the physico-chemical changes in cellular membranes allowing yeast cells to adapt to nutrient deficient conditions to maintain long-term viability.

Download Full-text