Exogenous abscisic acid (ABA) promotes cadmium (Cd) accumulation in Sedum alfredii Hance by regulating the expression of Cd stress response genes

AbstractThe F-box genes, which form one of the largest gene families in plants, are vital for plant growth, development and stress response. However, F-box gene family in Sedum alfredii remains unknown. Comprehensive studies addressing their function responding to cadmium stress is still limited. In the present study, 193 members of the F-box gene (SaFbox) family were identified, which were classified into nine subfamilies. Most of the SaFboxs had highly conserved domain and motif. Various functionally related cis-elements involved in plant growth regulation, stress and hormone responses were located in the upstream regions of SaFbox genes. RNA-sequencing and co-expression network analysis revealed that the identified SaFbox genes would be involved in Cd stress. Expression analysis of 16 hub genes confirmed their transcription level in different tissues. Four hub genes (SaFbox40, SaFbox51, SaFbox136 and SaFbox170) were heterologously expressed in a Cd-sensitive yeast cell to assess their effects on Cd tolerance. The transgenic yeast cells carrying SaFbox40, SaFbox51, SaFbox136, or SaFbox170 were more sensitive and accumulated more cadmium under Cd stress than empty vector transformed control cells. Our results performed a comprehensive analysis of Fboxs in S. alfredii and identified their potential roles in Cd stress response.

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Biochar and Glomus caledonium Influence Cd Accumulation of Upland Kangkong (Ipomoea aquatica Forsk.) Intercropped with Alfred Stonecrop (Sedum alfredii Hance)

Scientific Reports ◽

10.1038/srep04671 ◽

2014 ◽

Vol 4 (1) ◽

Cited By ~ 19

Author(s):

Junli Hu ◽

Fuyong Wu ◽

Shengchun Wu ◽

Cheung Lung Lam ◽

Xiangui Lin ◽

...

Keyword(s):

Sedum Alfredii ◽

Cd Accumulation ◽

Sedum Alfredii Hance ◽

Ipomoea Aquatica ◽

Glomus Caledonium

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Transcriptomic Analysis of Cadmium Stress Response in the Heavy Metal Hyperaccumulator Sedum alfredii Hance

PLoS ONE ◽

10.1371/journal.pone.0064643 ◽

2013 ◽

Vol 8 (6) ◽

pp. e64643 ◽

Cited By ~ 65

Author(s):

Jun Gao ◽

Ling Sun ◽

Xiaoe Yang ◽

Jian-Xiang Liu

Keyword(s):

Heavy Metal ◽

Stress Response ◽

Cadmium Stress ◽

Transcriptomic Analysis ◽

Sedum Alfredii ◽

Sedum Alfredii Hance

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Physiological and Transcriptomic Response of Grey Poplar (Populus ×Canescens Aiton Sm.) to Cadmium Stress

10.21203/rs.3.rs-48372/v1 ◽

2020 ◽

Author(s):

Martina Komarkova ◽

Jakub Chromy ◽

Eva Pokorna ◽

Petr Soudek ◽

Pavlina Machova

Keyword(s):

Stress Response ◽

Expression Profiles ◽

Translocation Factor ◽

Cadmium Stress ◽

Phenotypic Traits ◽

Cd Stress ◽

Transcriptomic Response ◽

Stress Response Genes ◽

Most Significant Change ◽

As Stress

Abstract Background: Populus ×canescens (Aiton) Sm. is a fast-growing woody plant belonging to the family Salicaceae. Thanks to its deep root system and fast growth rate, two poplar genotypes (TP11 and TP20) was chosen to be characterized and tested for a physiological and transcriptomic response to Cd stress.Results: Both sexual and clonal reproduction are common in grey poplar. Thus, to reveal its genetic structure, two selected poplar genotypes characterized by unique phenotypic traits were distinguished by microsatellite analysis. A comparative analysis of the effects of exposure to high cadmium (Cd) concentrations on the physiological response of TP11 and TP20 was performed. After 2 and 10 days, 10 µM and 100 µM Cd were added to the nutrient solution of poplar plants grown in perlite. Neither tested Cd concentration negatively affected plant growth; however, the chlorophyll content significantly decreased. According to mineral uptake analysis, the potassium (K) content was higher in the shoots than in the roots. The calcium (Ca) and magnesium (Mg) concentrations were only slightly affected by Cd treatment. The zinc (Zn) content in the shoots of TP20 was lower than that in the shoots of TP11. Moreover, the amount of Zn was slightly higher in the shoots of both genotypes after 10 days of Cd treatment compared to the control conditions. Cd accumulation was higher in the roots than in the shoots. After 2 days of 100 µM Cd treatment, TP11 accumulated significantly more Cd in the roots than TP20. After 10 days of exposure, 10 µM Cd resulted in comparable amounts of Cd in the roots and shoots of TP20, which was reflected in a translocation factor (TF) value greater than 1. Quantitative real-time RT-PCR analyses were used to examine the expression profiles of selected stress-response genes in the roots and shoots of plants exposed to Cd. The most significant change in transcript amount was observed in endochitinase 2, 12-oxophytodienoate reductase 1 and phi class glutathione S-transferase, which have been characterized as stress-response genes.Conclusions: Our study provided new insights for effective assessing the ability of different poplar genotypes to tolerate Cd stress and underlying Cd tolerance.

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AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis

Scientific Reports ◽

10.1038/s41598-020-80695-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yanxing Ma ◽

Hainan Tian ◽

Rao Lin ◽

Wei Wang ◽

Na Zhang ◽

...

Keyword(s):

Stress Response ◽

Protein Phosphatase ◽

Aba Signaling ◽

Response Gene ◽

Stress Response Genes ◽

Arabidopsis Protein ◽

Evolutionarily Conserved ◽

Increased Sensitivity ◽

Aba Response ◽

Transcription Repressors

AbstractExpression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression of stress response genes via ABA independent pathway. ABA induced transcription repressors (AITRs) are a family of novel transcription factors that play a role in ABA signaling, and Drought response gene (DRG) has previously been shown to play a role in regulating plant response to drought and freezing stresses. We report here the identification of DRG as a novel transcription factor and a regulator of ABA response in Arabidopsis. We found that the expression of DRG was induced by ABA treatment. Homologs searching identified AITR5 as the most closely related Arabidopsis protein to DRG, and homologs of DRG, including the AITR-like (AITRL) proteins in bryophytes and gymnosperms, are specifically presented in embryophytes. Therefore we renamed DRG as AITRL. Protoplast transfection assays show that AITRL functioned as a transcription repressor. In seed germination and seedling greening assays, the aitrl mutants showed an increased sensitivity to ABA. By using qRT-PCR, we show that ABA responses of some ABA signaling component genes including some PYR1-likes (PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs) and SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASES 2s (SnRK2s) were reduced in the aitrl mutants. Taken together, our results suggest that AITRLs are a family of novel transcription repressors evolutionally conserved in embryophytes, and AITRL regulates ABA response in Arabidopsis by affecting ABA response of some ABA signaling component genes.

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Transcriptional response of key metabolic and stress response genes of a nuculanid bivalve, Lembulus bicuspidatus from an oxygen minimum zone exposed to hypoxia-reoxygenation

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2021.110617 ◽

2021 ◽

pp. 110617

Author(s):

Katherine Amorim ◽

Helen Piontkivska ◽

Michael L. Zettler ◽

Eugene Sokolov ◽

Tjorven Hinzke ◽

...

Keyword(s):

Stress Response ◽

Transcriptional Response ◽

Oxygen Minimum Zone ◽

Stress Response Genes ◽

Minimum Zone ◽

Oxygen Minimum ◽

Hypoxia Reoxygenation

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Abscisic acid-mediated modifications in water transport continuum are involved in cadmium hyperaccumulation in Sedum alfredii

Chemosphere ◽

10.1016/j.chemosphere.2020.129339 ◽

2021 ◽

Vol 268 ◽

pp. 129339

Author(s):

Qi Tao ◽

Radek Jupa ◽

Qin Dong ◽

Xin Yang ◽

Yuankun Liu ◽

...

Keyword(s):

Abscisic Acid ◽

Water Transport ◽

Sedum Alfredii ◽

Cadmium Hyperaccumulation

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Auxin-Induced SaARF4 Downregulates SaACO4 to Inhibit Lateral Root Formation in Sedum alfredii Hance

International Journal of Molecular Sciences ◽

10.3390/ijms22031297 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1297

Author(s):

Dong Xu ◽

Zhuchou Lu ◽

Guirong Qiao ◽

Wenmin Qiu ◽

Longhua Wu ◽

...

Keyword(s):

Lateral Root ◽

Ethylene Biosynthesis ◽

Luciferase Assay ◽

Sedum Alfredii ◽

Ethylene Synthesis ◽

Root Tips ◽

Sedum Alfredii Hance ◽

Auxin Distribution ◽

Distribution Mode ◽

High Level

Lateral root (LR) formation promotes plant resistance, whereas high-level ethylene induced by abiotic stress will inhibit LR emergence. Considering that local auxin accumulation is a precondition for LR generation, auxin-induced genes inhibiting ethylene synthesis may thus be important for LR development. Here, we found that auxin response factor 4 (SaARF4) in Sedum alfredii Hance could be induced by auxin. The overexpression of SaARF4 decreased the LR number and reduced the vessel diameters. Meanwhile, the auxin distribution mode was altered in the root tips and PIN expression was also decreased in the overexpressed lines compared with the wild-type (WT) plants. The overexpression of SaARF4 could reduce ethylene synthesis, and thus, the repression of ethylene production decreased the LR number of WT and reduced PIN expression in the roots. Furthermore, the quantitative real-time PCR, chromatin immunoprecipitation sequencing, yeast one-hybrid, and dual-luciferase assay results showed that SaARF4 could bind the promoter of 1-aminocyclopropane-1-carboxylate oxidase 4 (SaACO4), associated with ethylene biosynthesis, and could downregulate its expression. Therefore, we concluded that SaARF4 induced by auxin can inhibit ethylene biosynthesis by repressing SaACO4 expression, and this process may affect auxin transport to delay LR development.

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