Genetic homogeneity assessment of in vitro-regenerated plantlets of Nyctanthes arbor-tristis L. and comparative evaluation of bioactive metabolites and antioxidant activity

2019 ◽  
Vol 56 (1) ◽  
pp. 72-87 ◽  
Author(s):  
Sobha Chandra Rath ◽  
Sen Seth ◽  
Sujit K. Mishra ◽  
Pawan K. Yadav ◽  
Aditya K. Gupta ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Comparative Evaluation ◽  
Genetic Homogeneity ◽  
Bioactive Metabolites ◽  
Homogeneity Assessment ◽  
Regenerated Plantlets

scholarly journals Effects of Processing on Polyphenolic and Volatile Composition and Fruit Quality of Clery Strawberries

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 632
Author(s):  
Stefania Garzoli ◽  
Francesco Cairone ◽  
Simone Carradori ◽  
Andrei Mocan ◽  
Luigi Menghini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Central Italy ◽  
In Vitro Assays ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Color Analysis ◽  
Food Ingredient ◽  
Red Color ◽  
The Impact

Strawberries belonging to cultivar Clery (Fragaria x ananassa (Duchesne ex Weston)), cultivated in central Italy were subjected to a multi-methodological experimental study. Fresh and defrosted strawberries were exposed to different processing methods, such as homogenization, thermal and microwave treatments. The homogenate samples were submitted to CIEL*a*b* color analysis and Head-Space GC/MS analysis to determine the impact of these procedures on phytochemical composition. Furthermore, the corresponding strawberry hydroalcoholic extracts were further analyzed by HPLC-DAD for secondary metabolites quantification and by means of spectrophotometric in vitro assays to evaluate their total phenolic and total flavonoid contents and antioxidant activity. These chemical investigations confirmed the richness in bioactive metabolites supporting the extraordinary healthy potential of this fruit as a food ingredient, as well as functional food, highlighting the strong influence of the processing steps which could negatively impact on the polyphenol composition. Despite a more brilliant red color and aroma preservation, non-pasteurized samples were characterized by a lower content of polyphenols and antioxidant activity with respect to pasteurized samples, as also suggested by the PCA analysis of the collected data.

In Vitro Comparative Evaluation of Antioxidant Activity of Hydrophobic and Hydrophilic Extracts from Algicolous Fungi

2016 ◽  
Vol 26 (1) ◽  
pp. 124-131 ◽  
Author(s):  
Awatief F. Hifney ◽  
Mustafa A. Fawzy ◽  
Khayria M. Abdel-Gawad ◽  
Ahmed A. Issa ◽  
Mohamed Gomaa
Keyword(s):  
Antioxidant Activity ◽  
Comparative Evaluation

scholarly journals Development of Synergy-Based Combination for Learning and Memory Using in vitro, in vivo and TLC-MS-Bioautographic Studies

2021 ◽  
Vol 12 ◽  
Author(s):  
Maaz Ahmed Khan ◽  
Varsha Srivastava ◽  
Mariya Kabir ◽  
Monalisha Samal ◽  
Areeba Insaf ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Learning And Memory ◽  
Anticholinesterase Activity ◽  
Bioactive Metabolites ◽  
Active Metabolites ◽  
White Spots ◽  
Synergistic Combination

The present study is aimed at developing a synergistic combination to enhance learning and memory in Alzheimer’s patients with the help of eight common medicinal plants used in the AYUSH system. Aqueous and hydroalcoholic extracts of eight medicinal plants from the AYUSH system of medicine were prepared. These were subjected to in vitro anticholinesterase activity, to find out the combination index of synergistic combination. The synergistic combination and their individual extracts were subjected to total phenol, flavonoid and antioxidant activity estimation. Further, in vivo neurobehavioral studies in rats were carried out followed by TLC-MS-bioautographic identification of bioactive metabolites. Out of the sixteen extracts, aqueous extracts of Withania somnifera (L.) Dunal (WSA) and Myristica fragrans (L.) Dunal (MFA) were selected for the development of synergistic combination based on their IC50 value in vitro anticholinesterase assay. The synergistic combination inhibited the anticholinesterase activity significantly as compared to the individual extracts of WSA and MFA. The synergistic combination also showed more phenolic and flavonoid contents with potential antioxidant activity. The TLC-bioautography showed four white spots in WSA, signifying sitoindosides VII, VIII, quercetin, isopelletierine and Withanolide S as AChE inhibitory compounds while showing five white spots of anti-cholinesterase active metabolites identified as eugenol, methyl eugenol, myristic acid, galbacin and β-sitosterol in MFA. The observation of neurocognitive behavior in amnesia induced subjects manifested that both the synergistic combinations showed comparable results to that of standard piracetam, though the synergistic combination containing a higher concentration of WSA showed more appreciable results in ameliorating dementia in rats. The study suggests that the synergy based combination successfully enhanced memory and learning by abating free radical and acetylcholine levels, and increased learning and memory in rats, providing a strong rationale for its use in the treatment of dementia and Alzheimer’s disease.

scholarly journals Elicitation of antioxidant metabolites in Musa species in vitro shoot culture using sucrose, temperature and jasmonic acid

2021 ◽  
Author(s):  
Ibukun O. Ayoola-Oresanya ◽  
Mubo A. Sonibare ◽  
Badara Gueye ◽  
Michael T. Abberton ◽  
Gertrud E. Morlock
Keyword(s):  
Antioxidant Activity ◽  
Jasmonic Acid ◽  
High Performance ◽  
Culture Medium ◽  
Shoot Culture ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Shoot Cultures ◽  
Alternative Means

AbstractIn vitro culture is not only known for the mass propagation of medicinal plants, it can also be used to boost the accumulation of useful metabolites. This work was aimed at studying the potential of elicitation on the accumulation of antioxidant secondary metabolites in different Musa species accessions. Shoot cultures of selected six Musa spp. accessions were micropropagated on Murashige and Skoog mineral-based culture medium, supplemented with indole-3-acetic acid and 6-benzylaminopurine. Different temperatures (15, 20 and 26 °C), doses of sucrose (30–50 g/L) and jasmonic acid concentrations (JA, 50–200 µM) were used as elicitors in the culture medium. High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis (EDA) was used to compare and characterize all the 71 Musa spp. samples from the selected accessions and to reveal compound zones with antioxidant, antidiabetic and anticholinesterase activities. All elicitation and plant tissue culture experiments increased the total phenolic content (TPC) and antioxidant activity of the selected Musa spp. accessions. Extracts from plants grown at 20 °C gave the highest TPC and antioxidant activity. Addition of JA (200 µM) and higher sucrose contents (40 g/L or 45 g/L) increased the TPC and antioxidant activity as well. Not only compounds with antioxidant, but also antidiabetic and anticholinesterase activities were detected in the elicited accessions. The system presented in this study could serve as a sustainable, consistent and reliable alternative means of bioactive metabolites supply for the production of biopharmaceuticals.

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