scholarly journals Elicitation of antioxidant metabolites in Musa species in vitro shoot culture using sucrose, temperature and jasmonic acid

2021 ◽  
Author(s):  
Ibukun O. Ayoola-Oresanya ◽  
Mubo A. Sonibare ◽  
Badara Gueye ◽  
Michael T. Abberton ◽  
Gertrud E. Morlock
Keyword(s):  
Antioxidant Activity ◽  
Jasmonic Acid ◽  
High Performance ◽  
Culture Medium ◽  
Shoot Culture ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Shoot Cultures ◽  
Alternative Means

AbstractIn vitro culture is not only known for the mass propagation of medicinal plants, it can also be used to boost the accumulation of useful metabolites. This work was aimed at studying the potential of elicitation on the accumulation of antioxidant secondary metabolites in different Musa species accessions. Shoot cultures of selected six Musa spp. accessions were micropropagated on Murashige and Skoog mineral-based culture medium, supplemented with indole-3-acetic acid and 6-benzylaminopurine. Different temperatures (15, 20 and 26 °C), doses of sucrose (30–50 g/L) and jasmonic acid concentrations (JA, 50–200 µM) were used as elicitors in the culture medium. High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis (EDA) was used to compare and characterize all the 71 Musa spp. samples from the selected accessions and to reveal compound zones with antioxidant, antidiabetic and anticholinesterase activities. All elicitation and plant tissue culture experiments increased the total phenolic content (TPC) and antioxidant activity of the selected Musa spp. accessions. Extracts from plants grown at 20 °C gave the highest TPC and antioxidant activity. Addition of JA (200 µM) and higher sucrose contents (40 g/L or 45 g/L) increased the TPC and antioxidant activity as well. Not only compounds with antioxidant, but also antidiabetic and anticholinesterase activities were detected in the elicited accessions. The system presented in this study could serve as a sustainable, consistent and reliable alternative means of bioactive metabolites supply for the production of biopharmaceuticals.

scholarly journals The Nutritional Value of Non-Traditional Gluten-Free Flakes and Their Antioxidant Activity

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 565
Author(s):  
Kristýna Šťastná ◽  
Martina Mrázková ◽  
Daniela Sumczynski ◽  
Betül Cındık ◽  
Erkan Yalçın
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Sugar Content ◽  
Chenopodium Quinoa ◽  
Biologically Active ◽  
Eragrostis Tef ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
In Vitro Digestibility

Nowadays, there is a growing interest for foods with a lower sugar content and rich in fiber and biologically active substances. The main purpose of this study was to prepare flakes from non-traditional pigmented cereals (Oryza sativa, Chenopodium quinoa, and Eragrostis tef) and to analyze their fibre, sugar, and in vitro digestibility values. Regarding phenolic antioxidants (soluble, soluble conjugated, and insoluble bound fractions), their content and antioxidant activity were measured using spectrophotometry and high performance liquid chromatography (HPLC) methods. Hydrothermally treated grains resulted in flakes with higher total dietary fibre contents (11.1–24.4%), quinoa and teff flakes were rich in maltose (up to 42.0 mg/g). Non-traditional flakes had lower in vitro digestibility, but conversely, they exhibited the highest phenolic contents corresponding with the highest antioxidant activity values (up to 2.33 mg Gallic acid equivalent/g of total phenolic content and 1.59 mg Trolox equivalent/g for 2,2-diphenyl-1-picrylhydrazyl (DPPH) in case of brown teff). Among free phenolics, the main contributors to an antioxidant activity were p-coumaric, o-coumaric, and gallic acids (r > 0.8186); among the soluble conjugated fractions, they were epigallocatechin, epicatechin, caffeic, and vanillic acids (r > 0.5935); while caffeic, protocatechuic, and ferulic acids (r > 0.5751) were the main contributors among the insoluble bound phenolics.

scholarly journals Optimization of phenolic compounds extraction with antioxidant activity from açaí, blueberry and goji berry using response surface methodology

2018 ◽  
pp. 180 ◽  
Author(s):  
Cristiane de Moura, Amália Soares dos Reis ◽  
Letícia Dangui da Silva, Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
Celeide Pereira ◽  
Solange Teresinha Carpes
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
High Performance ◽  
Total Phenolic ◽  
Global Response ◽  
Goji Berry ◽  
Berry Fruits

The aim of this study was to determine the best extraction conditions of phenolic compounds present in açaí, blueberry and goji berry fruits using the response surface methodology (RSM). The phenolic compounds profile by high-performance liquid chromatography, antioxidant activity was also determined. A factorial 23 design was used to analyze the effect of the solvent (ethanol and water), time (30 and 60 min) and temperature (30 °C and 60 °C) on the extraction of total phenolic compounds (TPC) and activity antioxidant (AA). The variables time and temperature had a positive effect on antioxidant activity (AA) in their highest levels, 60 min and 60 °C, respectively. The solvent ethanol 800 g/mL was more efficient in TPC extracting with AA in all matrices. Rutin was present in high amounts in blueberry and goji berry, and the myricetin in açaí. The açaí showed higher in vitro antioxidant activity when extracted at 60 °C for 60 min. The high correlation coefficient (0.98) of global response (GR) showed that we can find out single and global response in research with multiple dependent variables.  The GR analysis indicated the highest values of the TPC and AA when the fruits were extracted at 60°C for 60 min using ethanol as solvent and it was very useful for simplifying and improving the phenolic compounds extraction performance.

scholarly journals Effects of Processing on Polyphenolic and Volatile Composition and Fruit Quality of Clery Strawberries

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 632
Author(s):  
Stefania Garzoli ◽  
Francesco Cairone ◽  
Simone Carradori ◽  
Andrei Mocan ◽  
Luigi Menghini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Central Italy ◽  
In Vitro Assays ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Color Analysis ◽  
Food Ingredient ◽  
Red Color ◽  
The Impact

Strawberries belonging to cultivar Clery (Fragaria x ananassa (Duchesne ex Weston)), cultivated in central Italy were subjected to a multi-methodological experimental study. Fresh and defrosted strawberries were exposed to different processing methods, such as homogenization, thermal and microwave treatments. The homogenate samples were submitted to CIEL*a*b* color analysis and Head-Space GC/MS analysis to determine the impact of these procedures on phytochemical composition. Furthermore, the corresponding strawberry hydroalcoholic extracts were further analyzed by HPLC-DAD for secondary metabolites quantification and by means of spectrophotometric in vitro assays to evaluate their total phenolic and total flavonoid contents and antioxidant activity. These chemical investigations confirmed the richness in bioactive metabolites supporting the extraordinary healthy potential of this fruit as a food ingredient, as well as functional food, highlighting the strong influence of the processing steps which could negatively impact on the polyphenol composition. Despite a more brilliant red color and aroma preservation, non-pasteurized samples were characterized by a lower content of polyphenols and antioxidant activity with respect to pasteurized samples, as also suggested by the PCA analysis of the collected data.

scholarly journals Zinc as promoter of growth and biochemical activity in basil cultivars under in vitro conditions

2019 ◽  
pp. 342
Author(s):  
Caroline Galego Comar ◽  
Edinara Maria Barbosa ◽  
Vanusa Souza Rocha Pereira ◽  
Julliane Destro de Lima ◽  
Thiago Teodoro Santana ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Culture Medium ◽  
Total Phenolic ◽  
In Vitro Cultivation ◽  
Biochemical Activity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

In vitro cultivation of basil allows the manipulation of the concentration of certain micronutrients, commonly neglected by the micropropagation protocols. It is a plant of great economic importance for the cosmetic and pharmaceutical industry, due to the components present in its essential oil. In view of the above, the objective of this study was to evaluate zinc (Zn) concentrations in the micropropagation of basil, in addition to antioxidant activity and total phenolic compounds. Basil seeds, cultivars Manolo and Grecco Palla were oxygenated for 4 h, passed through asepsis and placed in test tubes with MS medium supplemented with 30 g L-1 sucrose and 6.5 g L-1 agar and pH adjusted to 5.8. The treatments were composed by the addition or not of 25 μM of zinc sulfate (ZnSO4) and arranged in a completely randomized design. The tubes containing the seeds and the culture medium were kept in a growth chamber for 90 days. The cultivar Manolo was more sensitive to the addition of ZnSO4 due to the increase in the number of leaves and in the antioxidant activity, however, the addition of this component in the culture medium did not influence the production of phenolic compounds or the activity of the antioxidant enzymes SOD, CAT and APX.

scholarly journals Eliminating Thrips From In Vitro Shoot Cultures of Apple with Insecticides

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Basdeo Bhagwat ◽  
W. David Lane
Keyword(s):  
Treatment Period ◽  
Culture Medium ◽  
Frankliniella Occidentalis ◽  
Shoot Culture ◽  
Shoot Cultures ◽  
Technical Grade ◽  
Dimethyl Phosphate ◽  
Apple Shoot ◽  
In Vitro Shoot Cultures

The insecticides acephate, dichlorvos, and imidacloprid were assayed, using in vitro shoot cultures of apple (Malus×domestica Borkh.), to determine their phytotoxicity at several concentrations and their effectiveness for eradication of the Western Flower Thrip (Frankliniella occidentalis, Pergande) from infested apple shoot cultures. Commercial formulations of acephate (Orthene), dichlorvos (VaportapeII), and imidacloprid (Admire) and a technical grade of imidacloprid were used in the experiments. For acephate and imidacloprid, concentrations of 1 to 80 mg·L-1 a.i. in shoot culture medium were used, while for dichlorvos, a fumigant, particles of the formulated product containing concentrations of 0.7 to 6.4 mg a.i. were suspended in the head space of the 500-mL glass culture jar. Acephate, dichlorvos, and the technical grade of imidacloprid did not cause phytotoxicity and growth of shoot cultures was unaffected at all treatment concentrations tested after a 6-week treatment period. Imidacloprid (20 to 80 mg·L-1 of the commercial formulation) caused chlorosis at the end of the 6-week treatment period. None of the treatments tested resulted in the death of shoots. Thrips were eradicated by acephate or imidacloprid treatments of 5 mg·L-1 and by dichlorvos treatment of 0.7 mg per 500-mL culture jar. Shoot cultures grew normally after the treatment period. Chemical names used: O,S-dimethyl acetylphosphoramidothioate (acephate), 2,2- dichlorovinyl dimethyl phosphate(dichlorvos),1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine (imidacloprid).

scholarly journals Antioxidant Activity and Antibacterial Effects on Clinical IsolatedStreptococcus suisandStaphylococcus intermediusof Extracts from Several Parts ofCladogynos orientalisand Their Phytochemical Screenings

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pongtip Sithisarn ◽  
Piyanuch Rojsanga ◽  
Patchima Sithisarn ◽  
Sumet Kongkiatpaiboon
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Performance ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Staphylococcus Intermedius

Thein vitroantioxidant and antibacterial assays against clinically isolatedStreptococcus suisandStaphylococcus intermediusof the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalisZipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted. Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC50values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity againstS. intermediuswith the inhibition zones between 7 and 14 mm. The chromatographic data suggested that the leaf extracts ofC. orientaliscontained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity againstStaphylococcus intermedius.

scholarly journals Free and Conjugated Phenolic Profiles and Antioxidant Activity in Quinoa Seeds and Their Relationship with Genotype and Environment

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1046
Author(s):  
Fabiana Antognoni ◽  
Giulia Potente ◽  
Stefania Biondi ◽  
Roberto Mandrioli ◽  
Lorenzo Marincich ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Environmental Conditions ◽  
High Performance ◽  
Biological Activities ◽  
Chenopodium Quinoa ◽  
Northern Italy ◽  
Temperate Climate ◽  
Total Phenolic ◽  
Array Detector

The nutraceutical interest in quinoa (Chenopodium quinoa Willd.) seeds is associated with the presence of macronutrients, micronutrients, minerals, vitamins, and polyphenols. In particular, polyphenols contribute to the health-promoting effects of this food crop, and their levels are influenced by environmental conditions. Production of quinoa is recently being explored in temperate climate areas, including Italy. The aim of this research was to assess the profile of bioactive compounds in seeds of two quinoa varieties, Regalona-Baer and Titicaca, grown in northern Italy, compared to that of seeds of those varieties grown in Chile and Denmark, respectively. High-performance liquid chromatography-diode array detector (HPLC-DAD) analysis of phenolic acid and flavonoid profiles, both in their free and soluble conjugated forms, showed that the main differences between Regalona grown in Chile and Italy were for the free vanillic acid and daidzein contents, while the two Titicaca samples mainly differed in quercetin derivative levels. The total phenolic index was comparable in Titicaca and Regalona, and only a slight decrease in this parameter was found in seeds of the two varieties grown in Italy. The in vitro antioxidant activity of seed extracts, evaluated by means of three different assays, indicated that it correlated with flavonol (quercetin derivative) levels. In conclusion, the results indicate that, although environmental conditions alter the polyphenolic profile and biological activities, it is possible to grow good-quality quinoa in northern Italy.

scholarly journals Phytochemical evaluation, HPTLC analysis and in-vitro antioxidant activity of hydroalcoholic leaf extract of Grewia hirsuta collected from Western Ghats forest

2020 ◽  
Vol 11 (3) ◽  
pp. 4653-4659
Author(s):  
Dattatraya Kature ◽  
Gaurav Gupta ◽  
Ritu Gilotra
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Western Ghats ◽  
High Performance ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

In-vitro antioxidant action of hydroalcoholic leaf extract of Grewia hirsuta (HAEGH) has been examined using one, “1-diphenyl-2-picryl-hydrazil (DPPH) free from radical scavenging” actions. The plant collected from the forest of the Western Ghats region of Karnataka province. The motive for plant collection from a specific location is the plant of forests exhibits the variation in growth, quantity, and quality of their active ingredients and secondary metabolites due to influence ecological factors like effect changes in location, soil, climate, etc. The work corresponds to preliminary phytochemical investigation for diverse phytoconstituents and quantitative phytochemical analysis of total phenolic, flavonoids & alkaloids content (TPC, TFC & TAC respectively) was evaluated with advanced methods. “HPTLC (High performance thin-layer” chromatography) fingerprint investigation was achieved for qualitative determination of the likely number of elements present in the hydroalcoholic extract. In-vitro antioxidant activity of HAEGH has been determined through hydroxyl radical scavenging assay that exhibited strong dose-dependent antioxidant activity as compared with standards compound, ascorbic acid. The IC50 value of HAEGH found, 25.90 % inhibition and for ascorbic acid, it was 17.68%. The Preliminary phytochemical estimation found presence of flavonoids, alkaloids, glycosides, phenol, proteins, diterpins and quantitative phytochemical analysis estimation of TPC, TFC & TAC found to be 3.627%, 4.059% & 5.671% respectively. HPTLC analysis of HAEGH at 354nm reveals the presence of a compound with Rf value 0.44 compare with Rf value 0.46 of quercetin. These outcomes indicated that the hydroalcoholic leaf extract of Grewia hirsuta plant contains phytoconstituents that exhibit antioxidant activity possible because of the existence of bioactive compounds.

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

Sign in / Sign up

Export Citation Format

Share Document