ThERF1 from Tamarix hispida confers decreased tolerance to oxidative and drought stresses and is regulated by a WRKY protein

Salt stress inhibits normal plant growth and development by disrupting cellular water absorption and metabolism. Therefore, understanding plant salt tolerance mechanisms should provide a theoretical basis for developing salt-resistant varieties. Here, we cloned ThTrx5 from Tamarix hispida, a salt-resistant woody shrub, and generated ThTrx5-overexpressing transgenic Arabidopsis thaliana lines. Under NaCl stress, the germination rate of overexpressing ThTrx5 lines was significantly increased relative to that of the nontransgenic line; under salt stress, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione levels and root length and fresh weight values of transgenic ThTrx5 plants were significantly greater than corresponding values for wild-type plants. Moreover, with regard to the transcriptome, comparison of differential gene expression of transgenic versus nontransgenic lines at 0 h and 3 h of salt stress exposure revealed 500 and 194 differentially expressed genes (DEGs), respectively, that were mainly functionally linked to catalytic activity and binding process. Pull-down experiments showed that ThTrx bound 2-Cys peroxiredoxin BAS1-like protein that influences stress response-associated redox, hormone signal transduction, and transcription factor functions. Therefore, this work provides important insights into ThTrx5 mechanisms that promote salt tolerance in plants.

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Heterologous expression of a plant WRKY protein confers multiple stress tolerance in E. coli Bir bitkinin heterolog ifadesi WRKY proteini çoklu stres yaratır E. coli’de tolerans

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0483 ◽

2020 ◽

Vol 45 (2) ◽

Author(s):

Farah Deeba ◽

Tasawar Sultana ◽

Nadia Majeed ◽

Syed Muhammad Saqlan Naqvi

Keyword(s):

Environmental Stresses ◽

Wrky Protein ◽

Stress Indicators ◽

Biotic Stresses ◽

E Coli ◽

Qpcr Analysis ◽

Defensive Role ◽

Stress Related Genes ◽

Multiple Stress Tolerance

AbstractObjectiveOsWRKY71, a WRKY protein from rice, is reported to function during biotic stresses. It is requisite to further enquire the efficiency and mechanism of OsWRKY71 under various environmental stresses. Stress indicators such as salt, cold, heat, and drought were studied by overexpressing the OsWRKY71 in E. coli.Materials and methodsDNA binding domain containing region of OsWRKY71 was cloned and expressed in E. coli followed by exposure to stress conditions. OsWRKY71 was also assessed for its role in abiotic stresses in rice by qPCR.ResultsRecombinant E. coli expressing OsWRKY71 was more tolerant to stresses such as heat, salt and drought in spot assay. The tolerance was further confirmed by monitoring the bacterial growth in liquid culture assay demonstrating that it encourages the E. coli growth under salt, drought, and heat stresses. This tolerance may be the consequence of OsWRKY71 interaction with the promoter of stress related genes or with other proteins in bacteria. The RT-qPCR analysis revealed the up-regulation of OsWRKY71 gene in rice upon interaction to cold, salt, drought and wounding with maximum up-regulation against salinity.ConclusionThus, the defensive role of OsWRKY71 may accord to the development and survival of plants during different environmental stresses.

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Vacuolar membrane H+-ATPase cˋˋ subunit gene (ThVHAcˋˋ1) from Tamarix hispida Willd improves salt stress tolerance

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2020.10.039 ◽

2020 ◽

Vol 157 ◽

pp. 370-378

Author(s):

Peilong Wang ◽

Yucong Guo ◽

Yuanyuan Wang ◽

Caiqiu Gao

Keyword(s):

Salt Stress ◽

Stress Tolerance ◽

Vacuolar Membrane ◽

Tamarix Hispida ◽

Subunit Gene ◽

Salt Stress Tolerance

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Genome-Wide Characterization, Expression Profile Analysis of WRKY Family Genes in Santalum album and Functional Identification of Their Role in Abiotic Stress

International Journal of Molecular Sciences ◽

10.3390/ijms20225676 ◽

2019 ◽

Vol 20 (22) ◽

pp. 5676 ◽

Cited By ~ 1

Author(s):

Haifeng Yan ◽

Mingzhi Li ◽

Yuping Xiong ◽

Jianming Wu ◽

Jaime A. Teixeira da Silva ◽

...

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Stress Responses ◽

Expression Profiles ◽

Expression Patterns ◽

Protein Sequences ◽

Santalum Album ◽

Biotic And Abiotic Stress ◽

Functional Identification ◽

Wrky Protein

WRKY proteins are a large superfamily of transcription factors that are involved in diverse biological processes including development, as well as biotic and abiotic stress responses in plants. WRKY family proteins have been extensively characterized and analyzed in many plant species, including Arabidopsis, rice, and poplar. However, knowledge on WRKY transcription factors in Santalum album is scarce. Based on S. album genome and transcriptome data, 64 SaWRKY genes were identified in this study. A phylogenetic analysis based on the structures of WRKY protein sequences divided these genes into three major groups (I, II, III) together with WRKY protein sequences from Arabidopsis. Tissue-specific expression patterns showed that 37 SaWRKY genes were expressed in at least one of five tissues (leaves, roots, heartwood, sapwood, or the transition zone), while the remaining four genes weakly expressed in all of these tissues. Analysis of the expression profiles of the 42 SaWRKY genes after callus was initiated by salicylic acid (SA) and methyl jasmonate (MeJA) revealed that 25 and 24 SaWRKY genes, respectively, were significantly induced. The function of SaWRKY1, which was significantly up-regulated by SA and MeJA, was analyzed. SaWRKY1 was localized in the nucleus and its overexpression improved salt tolerance in transgenic Arabidopsis. Our study provides important information to further identify the functions of SaWRKY genes and to understand the roles of SaWRKY family genes involved in the development and in SA- and MeJA-mediated stress responses.

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