Determination of suitability of black carrot (Daucus carota L. spp. sativus var. atrorubens Alef.) juice concentrate, cherry laurel (Prunus laurocerasus), blackthorn (Prunus spinosa) and red raspberry (Rubus ideaus) for kombucha beverage production

Various analytical approaches for determining β-carotene in vegetable crops and commercial dosage forms have been documented. However, neither the qualitative nor quantitative environmental safety and greener aspects of the literature analytical methodologies of β-carotene analysis have been assessed. As a result, the goal of this research is to develop and validate a reversed-phase “high-performance thin-layer chromatography (HPTLC)” approach for determining β-carotene in traditional (TE) and ultrasound-assisted (UBE) extracts of different fractions of Daucus carota (L.), Ipomea batatas (L.), and commercial formulation. The greener mobile phase for β-carotene analysis was a ternary mixture of ethanol, cyclohexane, and ammonia (95:2.5:2.5, v v v−1). The detection of β-carotene was done at a wavelength of 459 nm. In the 25–1000 ng band−1 range, the greener reversed-phase HPTLC approach was linear. Other validation factors for β-carotene analysis, including as accuracy, precision, robustness, and sensitivity, were likewise dependable. The contents of β-carotene were found to be maximum in hexane: acetone (50:50%) fractions of TE and UBE of D. carota and I. batatas compared to their acetone and hexane fractions. The amount of β-carotene in hexane: acetone (50:50%) portions of TE of D. carota, I. batatas and commercial formulation A was estimated to be 10.32, 3.73, and 6.73 percent w w−1, respectively. However, the amount of β-carotene in hexane: acetone (50:50%) portions of UBE of D. carota, I. batatas and commercial formulation A was estimated to be 11.03, 4.43, and 6.89 percent w w−1, respectively. The greenness scale for the proposed HPTLC strategy was calculated as 0.81 using the “analytical GREEnness (AGREE)” method, indicating that the proposed HPTLC methodology has good greenness. The UBE approach for extracting β-carotene outperformed the TE procedure. These results indicated that the greener reversed-phase HPTLC approach can be utilized for the determination of β-carotene in different vegetable crops, plant-based phytopharmaceuticals, and commercial products. In addition, this approach is also safe and sustainable due to the utilization of a greener mobile phase compared to the toxic mobile phases utilized in literature analytical approaches of β-carotene estimation.

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ULTRASOUND ASSISTED BIO-DYEING OF SOME TEXTILE MATERIALS WITH BLACK CARROT (DAUCUS CAROTA L.) EXTRACT

Cellulose Chemistry and Technology ◽

10.35812/cellulosechemtechnol.2021.55.32 ◽

2021 ◽

Vol 55 (3-4) ◽

pp. 325-342

Author(s):

HÜSEYIN BENLI

Keyword(s):

Daucus Carota ◽

Extraction Efficiency ◽

Metal Salts ◽

Test Results ◽

Light Fastness ◽

Black Carrot ◽

Daucus Carota L ◽

Textile Materials ◽

Ultrasonic Bath ◽

Extraction Volume

"This study has been conducted to find out whether black carrot extract can be used as a dyestuff to dye textile materials. For this purpose, the two most widely used textiles (namely, cotton and wool) have been selected. The extractions from the black carrot were performed directly in the dyebath. An ultrasonic bath at 80 °C for 60 minutes was used to increase extraction efficiency. All the dyeing processes were carried out at 100 °C for 60 minutes, both with and without some metal salts, using two different dye concentrations in 1:1 and 1:10 ratios (material weight:dye extraction volume). Six different metal salts, i.e. K2Cr2O7, CuSO45H2O, FeSO47H2O, SnCl22H2O, ZnSO47H2O, KAl(SO4)2.12H2O, were used in the dyeing processes, according to pre- and simultaneous mordanting methods. After the dyeing processes, the dyed samples’ CIE L*a*b* and color efficiency K/S values were determined by spectrophotometry. The SEM and FTIR data of the fabrics were collected and evaluated at the end of the trials as well. At the same time, perspiration, rubbing, washing and light fastness values of the dyed materials were also assessed. Acceptable fastness test results were obtained for the dyed samples. The findings of the study indicated that the dyeability performance of wool and cotton fabrics with black carrot (Daucus carota L.) extract could be greatly influenced by the mordant used and its application method."

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Antioxidant activities of roots, leaves, and stems of carrot (Daucus carota L.) using DPPH and FRAP methods

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4570 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 2856-2863

Author(s):

Hartati R ◽

Amalina M N ◽

Fidrianny I

Keyword(s):

Free Radicals ◽

Ethyl Acetate ◽

Daucus Carota ◽

Antioxidant Activities ◽

Chemical Species ◽

Total Phenolic ◽

Positive Correlation ◽

Daucus Carota L ◽

Ethanol Determination

Free radicals are chemical species with unpaired electrons in their outer orbital that can attack other molecules, causing cell oxidative damage and degenerative diseases. Free radicals can be prevented by antioxidant. An antioxidant can be found in nature as secondary metabolites in plants, such as carrot (Daucus carota L.). This research was conducted to study the antioxidant activities of roots, leaves, and stems of carrot extracts using DPPH and FRAP methods, determine total phenolic content (TPC) and total flavonoid content (TFC), analyse the correlation between TPC and TFC with AAI DPPH and FRAP, and the correlation between two methods. The sample was extracted by reflux using n-hexane, ethyl acetate, and ethanol. Determination of TPC, TFC, AAI DPPH and FRAP was performed using UV-visible spectrophotometry. Correlation of TPC and TFC with AAI DPPH and FRAP and also the correlation between the two methods were conducted using Pearson's method. Ethyl acetate carrot leaves extract showed the highest TPC and TFC (8.88 ± 0.44 g GAE/100 g and 9.00 ± 0.31 g QE/100 g). AAI DPPH of carrot extract in the range of 0.16 – 1.42, meanwhile AAI FRAP 1.89 – 5.45. TPC and TFC of carrot roots extract showed a significantly positive correlation with AAI DPPH and FRAP. AAI DPPH and FRAP of carrot roots extract gave a significantly positive correlation. Ethyl acetate and ethanol carrot leave extracts were strong to very strong antioxidant by DPPH and FRAP methods. TPC and TFC in carrot roots extract contributed to antioxidant activities by DPPH and FRAP. DPPH and FRAP presented linear results in antioxidant activities of carrot roots extract.

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