Improvement of shoot morphogenesis in vitro and assessment of changes of the activity of antioxidant enzymes during acclimation of micropropagated plants of Desert Teak

2011 ◽  
Vol 34 (3) ◽  
pp. 859-867 ◽  
Author(s):  
Ankita Varshney ◽  
Mohammad Anis
Keyword(s):  
Antioxidant Enzymes ◽  
Shoot Morphogenesis ◽  
Morphogenesis In Vitro ◽  
Micropropagated Plants

scholarly journals STUDY ON SHOOT MORPHOGENESIS IN-VITRO OF MORINDA CITRIFOLIA L.

2010 ◽  
Vol 13 (1) ◽  
pp. 48-53
Author(s):  
Huong Thi Ngoc Nguyen ◽  
Mai Thi Bach Vo
Keyword(s):  
Root Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Root Formation ◽  
Morinda Citrifolia ◽  
Endogenous Hormones ◽  
Adventitious Shoot Formation ◽  
Shoot Morphogenesis ◽  
Morphogenesis In Vitro

Morinda citrifolia L. is a valuable medicinal plant, used to treat many diseases, such as sleeplessness, backache, high blood pressure... To study the shoot morphogenesis in Morinda citrifolia L. for propagation in the future, we examined the effects of BA, Zeatin and NAA on adventitious shoot formation of hypocotyl. The results showed that the regeneration of adventitious shoot comprised three steps of morphogenesis. In this process, Zeatin 1mg/l stimulated the formation of shoot primordia in dark (after a week), earlier than BA at the same concentration. Shoot generation was slow (after five weeks) on MS medium supplemented with 0,1mg/l and Zeatin 1mg/l. Roles of respiration rate and endogenous hormones were discussed to understand the physiological changes in the adventitious root formation.

Micro-Structural Stability of Micropropagated Plants of Vitex negundo L.

2021 ◽  
pp. 1-9
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
Mahipal S. Shekhawat
Keyword(s):  
Structural Changes ◽  
Growth Conditions ◽  
Culture Industry ◽  
Vascular Bundles ◽  
Vitex Negundo ◽  
Structural Variations ◽  
Structural Repair ◽  
Micropropagated Plants ◽  
Palisade Cells

Abstract Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

scholarly journals Soybean (Glycine max) Is Able to Absorb, Metabolize and Accumulate Fenbendazole in All Organs Including Beans

2021 ◽  
Vol 22 (13) ◽  
pp. 6647
Author(s):  
Radka Podlipná ◽  
Martina Navrátilová ◽  
Lucie Raisová Stuchlíková ◽  
Kateřina Moťková ◽  
Lenka Langhansová ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Glycine Max ◽  
Organic Compounds ◽  
Animal Feed ◽  
Significant Risk ◽  
Plant Metabolism ◽  
Plant Fitness ◽  
Human Food ◽  
Food Consumed

Although manure is an important source of minerals and organic compounds it represents a certain risk of spreading the veterinary drugs in the farmland and their permeation to human food. We tested the uptake of the anthelmintic drug fenbendazole (FBZ) by soybean, a common crop plant, from the soil and its biotransformation and accumulation in different soybean organs, including beans. Soybeans were cultivated in vitro or grown in a greenhouse in pots. FBZ was extensively metabolized in roots of in vitro seedlings, where sixteen metabolites were identified, and less in leaves, where only two metabolites were found. The soybeans in greenhouse absorbed FBZ by roots and translocated it to the leaves, pods, and beans. In roots, leaves, and pods two metabolites were identified. In beans, FBZ and one metabolite was found. FBZ exposure did not affect the plant fitness or yield, but reduced activities of some antioxidant enzymes and isoflavonoids content in the beans. In conclusion, manure or biosolids containing FBZ and its metabolites represent a significant risk of these pharmaceuticals entering food consumed by humans or animal feed. In addition, the presence of these drugs in plants can affect plant metabolism, including the production of isoflavonoids.

scholarly journals Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

2013 ◽  
Vol 19 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Jyoti R. Rout ◽  
Shidharth S. Ram ◽  
Ritarani Das ◽  
Anindita Chakraborty ◽  
Mathummal Sudarshan ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Withania Somnifera ◽  
Protein Profile ◽  
Copper Stress ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

The mechanism of mouse egg-cylinder morphogenesis in vitro

Development ◽  
1981 ◽  
Vol 61 (1) ◽  
pp. 277-287
Author(s):  
A. J. Copp
Keyword(s):  
Giant Cell ◽  
Cell Formation ◽  
Cell Movement ◽  
Giant Cells ◽  
Cell Number ◽  
Dependent Change ◽  
Morphogenesis In Vitro ◽  
Trophoblast Giant Cells

The number of trophoblast giant cells in outgrowths of mouse blastocysts was determined before, during and after egg-cylinder formation in vitro. Giant-cell numbers rose initially but reached a plateau 12 h before the egg cylinder appeared. A secondary increase began 24 h after egg-cylinder formation. Blastocysts whose mural trophectoderm cells were removed before or shortly after attachment in vitro formed egg cylinders at the same time as intact blastocysts but their trophoblast outgrowths contained fewer giant cells at this time. The results support the idea that egg-cylinder formation in vitro is accompanied by a redirection of the polar to mural trophectoderm cell movement which characterizes blastocysts before implantation. The resumption of giant-cell number increase in trophoblast outgrowths after egg-cylinder formation may correspond to secondary giant-cell formation in vivo. It is suggested that a time-dependent change in the strength of trophoblast cell adhesion to the substratum occurs after blastocyst attachment in vitro which restricts the further entry of polar cells into the outgrowth and therefore results in egg-cylinder formation.

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