Antioxidant enzyme activity and microRNA are associated with growth of Poa pratensis callus under salt stress

2020 ◽  
Vol 14 (4) ◽  
pp. 429-438
Author(s):  
Hongsong Luo ◽  
Zhixiang Zhou ◽  
Guilong Song ◽  
Hongxiang Yao ◽  
Liebao Han
Keyword(s):  
Enzyme Activity ◽  
Salt Stress ◽  
Antioxidant Enzyme ◽  
Poa Pratensis ◽  
Antioxidant Enzyme Activity

scholarly journals The ThSOS3 Gene Improves the Salt Tolerance of Transgenic Tamarix hispida and Arabidopsis thaliana

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhongyuan Liu ◽  
Qingjun Xie ◽  
Feifei Tang ◽  
Jing Wu ◽  
Wenfang Dong ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Antioxidant Enzyme ◽  
Electrolyte Leakage ◽  
Transgenic Arabidopsis ◽  
Antioxidant Enzyme Activity ◽  
Tamarix Hispida ◽  
Ros Scavenging ◽  
Salt Stress Response

The salt overly sensitive (SOS) signal transduction pathway is one of the most highly studied salt tolerance pathways in plants. However, the molecular mechanism of the salt stress response in Tamarix hispida has remained largely unclear. In this study, five SOS genes (ThSOS1–ThSOS5) from T. hispida were cloned and characterized. The expression levels of most ThSOS genes significantly changed after NaCl, PEG6000, and abscisic acid (ABA) treatment in at least one organ. Notably, the expression of ThSOS3 was significantly downregulated after 6 h under salt stress. To further analyze ThSOS3 function, ThSOS3 overexpression and RNAi-mediated silencing were performed using a transient transformation system. Compared with controls, ThSOS3-overexpressing transgenic T. hispida plants exhibited greater reactive oxygen species (ROS)-scavenging capability and antioxidant enzyme activity, lower malondialdehyde (MDA) and H2O2 levels, and lower electrolyte leakage rates under salt stress. Similar results were obtained for physiological parameters in transgenic Arabidopsis, including H2O2 and MDA accumulation, superoxide dismutase (SOD) and peroxidase (POD) activity, and electrolyte leakage. In addition, transgenic Arabidopsis plants overexpressing ThSOS3 displayed increased root growth and fresh weight gain under salt stress. Together, these data suggest that overexpression of ThSOS3 confers salt stress tolerance on plants by enhancing antioxidant enzyme activity, improving ROS-scavenging capability, and decreasing the MDA content and lipid peroxidation of cell membranes. These results suggest that ThSOS3 might play an important physiological role in salt tolerance in transgenic T. hispida plants. This study provides a foundation for further elucidation of salt tolerance mechanisms involving ThSOSs in T. hispida.

Nitric Oxide Enhances Salt Tolerance through Regulating Antioxidant Enzyme Activity and Nutrient Uptake in Pea

2020 ◽  
Author(s):  
Esin Dadasoglu ◽  
Melek Ekinci ◽  
Raziye Kul ◽  
Mostafakamal Shams ◽  
Metin Turan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Salt Stress ◽  
Nutrient Uptake ◽  
Antioxidant Enzyme ◽  
Crop Production ◽  
Electrical Conductance ◽  
Antioxidant Enzyme Activity ◽  
Stress Factors ◽  
The Impact

Background: Salinity is one of the environmental stress factors that restrict the crop production by endangering agricultural areas. Nitric oxide (NO) protects plants from damage caused by oxidative stress conditions in various biological ways. Methods: In this greenhouse investigation during 2018, pea plants were irrigated with three levels of NaCl (0, 50 and 100 mM) solutions. NO solutions were prepared with three different doses (0, 75 and 100 µM SNP). These solutions were applied to the seeds before sowing and then to the leaves of the pea cultivars. The study was conducted to analyze the impact of NO on growth, malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzyme activity and nutrient uptake in two pea cultivars under salinity conditions. Result: Salinity reduced fresh-dry weight, relative water content (RWC), and chlorophyll a and b content of pea. However, NO enhanced these parameters under salt stress. Salinity increased tissue electrical conductance (TEC), H2O2 and MDA content, which were decreased by combined application of NaCl and NO. Salinity caused an increase in antioxidant enzyme activity in pea and NO made a significant improvement in their activities under salinity conditions. Salinity treatments decreased the ratio of K+/Na+ and Ca2+/Na+ in both cultivars, and application of NO elevated them as compared to the control under salt stress. In conclude, exogenous NO treatment could help pea to tolerate salinity stress by increasing the chlorophyll content and regulating antioxidant enzyme activity and nutrient uptake.

scholarly journals Divergences in Hormonal and Enzymatic Antioxidant Responses of Two Chicory Ecotypes to Salt Stress1

2016 ◽  
Vol 34 (2) ◽  
pp. 199-208 ◽  
Author(s):  
K. GHANAATIAN ◽  
H. SADEGHI
Keyword(s):  
Enzyme Activity ◽  
Salt Stress ◽  
Seed Germination ◽  
Antioxidant Enzyme ◽  
Tap Water ◽  
Crop Productivity ◽  
Antioxidant Enzyme Activity ◽  
Randomized Design ◽  
Germination Characteristics ◽  
Aba Content

ABSTRACT Salinity is among the most common and severe abiotic stresses that drastically affects crop productivity all over the world. To evaluate the effect of salt stress on seed germination, early growth, antioxidant enzyme activity and ABA content of chicory ecotypes (Cichorium intybus), a factorial experiment was conducted at College of Agriculture, Shiraz University in 2014 based on completely randomized design with four replications. The treatments comprised five salinity levels (tap water, 3, 6, 9, 12 dS m-1) of sodium chloride on the ecotypes of Sefid Shiraz and Siyah Shiraz. The results showed that germination characteristics and primary seedling growth decreased in both ecotypes with increasing in salinity severity. The effects of salinity on radicle and plumule length as well as seedling weight were the same as its effects on seed germination. The effect of salt stress on antioxidant enzyme activity (especially catalase) and ABA content were significant which they were enhanced with increasing salinity level; the Siyah Shiraz ecotype performs better than the Sefid Shiraz under high salinity, as indicated by lower decrease in germination characteristics and primary growth and higher antioxidant enzyme activity as well as ABA content. These facts should be taken into consideration in the economic cultivation of this valuable horticultural and medicinal plant and this data would be useful for the crop breeding projects.

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