scholarly journals Combining Bone Collagen Matrix with hUC-MSCs for Application to Alveolar Process Cleft in a Rabbit Model

2021 ◽  
Author(s):  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Dan Zhang ◽  
Jian-Hui Li ◽  
Li-Qiang Yin ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Collagen Matrix ◽  
Bone Collagen ◽  
Alveolar Process

Application of Bone Collagen Matrix combined with HUC-MSCs for Alveolar Process Cleft in a Rabbit Model

2021 ◽  
Author(s):  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Dan Zhang ◽  
Jian-Hui Li ◽  
Li-Qiang Yin ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Collagen Matrix ◽  
Bone Defects ◽  
Histochemical Staining ◽  
Blood Analysis ◽  
Bone Collagen ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Alveolar Process ◽  
Materials Used

Abstract Background: Most materials used clinically for filling severe bone defects lack the ability to induce bone regeneration or low bone conversion rate, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (HUC-MSCs) have good osteoinduction. The mechanism of heterogeneous bone collagen matrix combined with HUC-MSCs in repairing bone defects of alveolar process cleft is still unclear.Methods: Here, a rabbit alveolar process cleft model was established to evaluate the repair effect and molecular mechanism of bone collagen matrix and its combination with HUC-MSCs on critical-sized bone defects in the alveolar process. We randomly divided 48 young Japanese white rabbits (JWRs) into normal, control, material and MSCs groups. The alveolar process cleft model was established by removing the bone of equal volume at the left maxillary. Bone collagen matrix combined with HUC-MSCs that were then implanted in the defect area. X-ray analysis and blood analysis were analyzed 3 months after surgery. Skull tissues were collected for micro-focus computerized tomography (micro-CT) analysis and histochemical staining. The above experiment was repeated 6 months after surgery.Results: the main findingsResults: It was found that bone collagen matrix and HUC-MSCs showed good biocompatibility. Heterogeneous bone collagen matrix combined with HUC-MSCs significantly enhanced osteoinduction and osteoconduction. Conclusions: This experiment provides a new method for repairing alveolar process cleft.Trial registration: Not applicable.

Lumbar Spinal Fusion With a Mineralized Collagen Matrix and rhBMP-2 in a Rabbit Model

Spine ◽  
2003 ◽  
Vol 28 (17) ◽  
pp. 1954-1960 ◽  
Author(s):  
S. S. Liao ◽  
K. Guan ◽  
F. Z. Cui ◽  
S. S. Shi ◽  
T. S. Sun
Keyword(s):  
Spinal Fusion ◽  
Rabbit Model ◽  
Collagen Matrix ◽  
Lumbar Spinal Fusion ◽  
Lumbar Spinal ◽  
Mineralized Collagen

The Role of Water in Bone Nanohydroxyapatite Nucleation

2020 ◽  
Vol 12 (5) ◽  
pp. 576-581
Author(s):  
Rodolfo J. González-Paz ◽  
Karem Noris-Suárez ◽  
José L. Feijoo ◽  
Gema Gonzalez
Keyword(s):  
Electrostatic Interactions ◽  
Bone Cells ◽  
Bone Matrix ◽  
Collagen Matrix ◽  
Bone Collagen ◽  
Collagen Molecule ◽  
Mineral Phase ◽  
Structural Water ◽  
Phosphate Ions ◽  
New Interactions

In a previous study of the deposition of nanohydroxyapatite on demineralized cortical bone collagen stimulated by deformation-induced piezoelectricity was obtained in our lab. Thus, the aim of the present study was to characterize the interactions between the self-assembled nanostructure of bone collagen and water in presence and absence of hydroxyapatite (HAP) to elucidate which factors allowed such deposition in just four weeks without the presence of bone cells. It was found that the loss of the mineral phase in bone (HAP) contributes to decrease the thermal stability of the collagen nanostructures. An important increase on the free, bonded and structural water content was found in the bone matrix, directly related to the degree of demineralization reached by the collagen matrix with time. This demonstrates that the collagen nanostructure is able to restructure itself while losing the interactions with the mineral phase, establishing new interactions with the water molecule, orienting the dipoles of the collagen molecule. All these increase the piezoelectric character of the bone matrix and allows the collagen matrix to get prepared for a bone re-mineralization process without bone cells, due to the electrostatic interactions established with the calcium and phosphate ions of the external fluid of the extracellular matrix.

scholarly journals The Effect of a Triple Combination of Bevacizumab, Sodium Hyaluronate and a Collagen Matrix Implant in a Trabeculectomy Animal Model

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 896
Author(s):  
Vanessa Andrés-Guerrero ◽  
Irene Camacho-Bosca ◽  
Liseth Salazar-Quiñones ◽  
Nestor Ventura-Abreu ◽  
Mercedes Molero-Senosiain ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Rabbit Model ◽  
Sodium Hyaluronate ◽  
Collagen Matrix ◽  
New Combination ◽  
Wound Healing Process ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Fibroblast Migration

Currently available anti-scarring treatments for glaucoma filtration surgery (GFS) have potentially blinding complications, so there is a need for alternative and safer agents. The effects of the intrableb administration of a new combination of the anti-VEGF bevacizumab, sodium hyaluronate and a collagen matrix implant were investigated in a rabbit model of GFS, with the purpose of modulating inflammation, angiogenesis, fibroblast migration and fibrogenesis in the wound healing process. A comparative-effectiveness study was performed with twenty-four rabbits, randomly assigned to the following treatments: (a) biodegradable collagen matrix implant (Olo), (b) bevacizumab-loaded collagen matrix implant (Olo-BVZ), (c) bevacizumab-loaded collagen matrix implant combined with sodium hyaluronate (Olo-BVZ-H5) and (d) sham-operated animals (control). Rabbits underwent a conventional trabeculectomy and were studied over 30 days in terms of intraocular pressure and bleb characterization (height, area and vascularity in central, peripheral and non-bleb zones). Histologic differences among groups were further evaluated at day 30 (inflammation, total cellularity and degree of fibrosis in the area of surgery). Local delivery of bevacizumab (Olo-BVZ and Olo-BVZ-H5) increased the survival of the filtering bleb by 21% and 31%, respectively, and generated a significant decrease in inflammation and cell infiltration histologically 30 days after surgery, without exhibiting any local toxic effects. Olo-BVZ-H5 showed less lymphocyte infiltration and inflammation than the rest of the treatments. Intraoperative intrableb implantation of bevacizumab, sodium hyaluronate and a collagen matrix may provide an improved trabeculectomy outcome in this model of intense wound healing. This study showed an effective procedure with few surgical complications and a novel combination of active compounds that offer new possibilities to improve the efficacy of filtration surgery.

scholarly journals Engaging a polylactide copolymer in oral tissue regeneration: first validation of Suprathel® for guided epithelial and osseous healing

2021 ◽  
Vol 14 (2) ◽  
pp. 181-197
Author(s):  
Sergiu Vacaras ◽  
◽  
◽  
◽  
◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Rabbit Model ◽  
Collagen Matrix ◽  
Skin Regeneration ◽  
Oral Epithelium ◽  
Trimethylene Carbonate ◽  
Clinical Reason ◽  
Oral Tissue ◽  
Future Evaluation

The present study investigated the capacity of Suprathel® (a copolymer membrane, so far validated for skin regeneration) to also regenerate oral tissue – mucosa and bone, by comparing this biomaterial, in a split-mouth rabbit model, to Mucoderm®, a xenogeneic collagen matrix certified for keratinized oral mucosa healing. The clinical reason behind this experimental animal model was to determine whether the benefits of this advanced skin regeneration product (Suprathel®) could be conveyed for future evaluation in clinical trials of oral tissue regeneration in humans. The outcomes of this study validated the use of Suprathel®, a terpolymer of polylactide with trimethylene carbonate and ε-caprolactone, for stimulation of oral epithelium and alveolar bone regeneration in rabbits. Both Suprathel® and Mucoderm® exhibited comparable results and the null hypothesis stating a comparable regenerating effect of these two materials could not be rejected.

scholarly journals A 3-Year Prospective Study on a Porcine-Derived Acellular Collagen Matrix to Re-Establish Convexity at the Buccal Aspect of Single Implants in the Molar Area: A Volumetric Analysis

2020 ◽  
Vol 9 (5) ◽  
pp. 1568 ◽  
Author(s):  
Célien Eeckhout ◽  
Eline Bouckaert ◽  
Dagmar Verleyen ◽  
Thomas De Bruyckere ◽  
Jan Cosyn
Keyword(s):  
Soft Tissue ◽  
Soft Tissues ◽  
Case Series ◽  
Collagen Matrix ◽  
Linear Increase ◽  
Alveolar Process ◽  
Wide Diameter ◽  
Area Of Interest ◽  
Soft Tissue Volume ◽  
Soft Tissue Profile

Background: Xenogeneic soft tissue substitutes are currently being investigated as an alternative to subepithelial connective tissue grafts (CTG) with the intention to avoid postoperative morbidity associated with autologous grafting. The aim of the present study was to volumetrically evaluate the effectiveness and mid-long-term stability of a porcine-derived collagen matrix (PDCM) (Mucoderm®, Botiss gmbh, Berlin, Germany) in increasing soft tissue volume at the buccal aspect of molar implant sites. Methods: Periodontally healthy non-smoking patients with a single tooth gap in the molar area were selected for a prospective case series. All sites had a bucco-oral bone dimension of at least 8 mm and demonstrated a horizontal alveolar defect. A wide diameter implant was placed under the elevated buccal flap and a PDCM was applied. The primary outcome was the linear increase in buccal soft tissue profile (BSP) within a well-defined area of interest. This was performed with designated software (SMOP; Swissmeda AG, Zurich, Switzerland) on the basis of superimposed digitalized study casts taken before surgery (T0), immediately after surgery (T1), at three months (T2), one year (T3) and three years (T4). Secondary outcomes were alveolar process deficiency and clinical parameters. Results: Fourteen out of 15 treated patients attended the three-year re-assessment (four females; mean age 51.4 years). Mean linear increase in BSP at T1 was 1.53 mm (p = 0.001). The PDCM showed substantial resorption at T2 (1.02 mm or 66.7%) (p = 0.001). Thereafter, a 0.66 mm volume gain was observed (p = 0.030), possibly due to the installation of a permanent crown displacing the soft tissues to the buccal aspect. This resulted in a linear increase in BSP of 1.17 mm (76.5%) at T4. Alveolar process deficiency significantly reduced over time (p = 0.004). However, 50% of patients still demonstrated a slight (6/14) or obvious (1/14) alveolar process deficiency at study termination. Implants demonstrated healthy clinical conditions. Conclusions: The PDCM demonstrated marked resorption during the early stages of healing. Due to the matrix thickening the tissues, and the permanent crown displacing the tissues, 76.5% of the initial increase in BSP could be maintained over a three-year period. Half of the patients failed to show perfect soft tissue convexity at the buccal aspect.

Efficacy of collagen matrix implant on adhesions in restrictive strabismus: An experimental study in a rabbit model

2018 ◽  
Vol 97 (2) ◽  
Author(s):  
Yung Ju Yoo ◽  
Jeong‐Min Hwang ◽  
Gheeyoung Choe ◽  
Hee Kyung Yang
Keyword(s):  
Experimental Study ◽  
Rabbit Model ◽  
Collagen Matrix ◽  
Restrictive Strabismus
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