scholarly journals Spatiotemporal expression of periostin during skin development and incisional wound healing: lessons for human fibrotic scar formation

2010 ◽  
Vol 4 (2) ◽  
pp. 99-107 ◽  
Author(s):  
Hong-Ming Zhou ◽  
Jian Wang ◽  
Christopher Elliott ◽  
Weiyan Wen ◽  
Douglas W. Hamilton ◽  
...  
Keyword(s):  
Wound Healing ◽  
Scar Formation ◽  
Skin Development ◽  
Spatiotemporal Expression ◽  
Fibrotic Scar

scholarly journals Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Zhou ◽  
Ludvig J. Backman ◽  
Patrik Danielson
Keyword(s):  
Wound Healing ◽  
Mitochondrial Dna ◽  
Scar Formation ◽  
Mitochondrial Rna ◽  
Peripheral Part ◽  
Double Stranded Rna ◽  
Corneal Wound ◽  
Biochemical Approach ◽  
Common Deletion ◽  
Underlying Mechanisms

AbstractScar formation as a result of corneal wound healing is a leading cause of blindness. It is a challenge to understand why scar formation is more likely to occur in the central part of the cornea as compared to the peripheral part. The purpose of this study was to unravel the underlying mechanisms. We applied RNA-seq to uncover the differences of expression profile in keratocytes in the central/peripheral part of the cornea. The relative quantity of mitochondrial RNA was measured by multiplex qPCR. The characterization of mitochondrial RNA in the cytoplasm was confirmed by immunofluoresence microscope and biochemical approach. Gene expression was analyzed by western blot and RT qPCR. We demonstrate that the occurrence of mitochondrial DNA common deletion is greater in keratocytes from the central cornea as compared to those of the peripheral part. The keratocytes with CD have elevated oxidative stress levels, which leads to the leakage of mitochondrial double-stranded RNA into the cytoplasm. The cytoplasmic mitochondrial double-stranded RNA is sensed by MDA5, which induces NF-κB activation. The NF-κB activation thereafter induces fibrosis-like extracellular matrix expressions and IL-8 mRNA transcription. These results provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

RNA Sequencing Reveals the Wound Repair Mechanism of Cuyuxunxi Prescription in Surgical Patients with Anal Fistulas

2021 ◽  
Vol 24 ◽  
Author(s):  
Yin Qu ◽  
Zhijun Zhang ◽  
Yafeng Lu ◽  
De Zheng ◽  
Wei Yang
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Rna Sequencing ◽  
Anal Fistula ◽  
Wound Repair ◽  
Inflammatory Responses ◽  
Control Group ◽  
Surgical Wound ◽  
Anal Fistulas ◽  
Skin Development

Background: Anal fistula is one of the most common colorectal and perirectal diseases in the world. Cuyuxunxi (CYXX) prescription is an efficient herbal fumigant used to promote the surgical wound healing of anal fistulas. Objective: This study aimed to explore the underlying molecular mechanism of CYXX prescription on surgical wound healing of anal fistulas. Methods: Ten patients with anal fistula were randomized into a control group or treatment group. The wound surface of patients in the control group was rinsed by normal saline, while that in the treatment group was rinsed by CYXX prescription. The wound tissues of patients with anal fistulas seven days after the surgery were collected for hematoxylin-eosin (HE) staining and RNA sequencing. The expressions of differentially expressed genes (DEGs) were validated by real-time quantitative PCR (RT-qPCR). Results: HE staining showed that CYXX treatment reduced the infiltration of inflammatory cells. A total of 472 DEGs, including 141 up-regulated genes and 331 down-regulated genes, were identified. These genes were significantly related to skin development, xenobiotic stimulus, and inflammation. In addition, the consistency rate of RT-qPCR and sequencing results was 83.33%, which showed a high relative reliability of the sequencing results. Conclusion: CYXX prescription could improve epidermis repair and reduce inflammatory responses.

scholarly journals Epigenetic Modification Prevents Excessive Wound Healing and Scar Formation After Glaucoma Filtration Surgery

2016 ◽  
Vol 57 (7) ◽  
pp. 3381 ◽  
Author(s):  
Ajay Sharma ◽  
Govindaraj Anumanthan ◽  
Marcos Reyes ◽  
Huiyi Chen ◽  
Jacob W. Brubaker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epigenetic Modification ◽  
Scar Formation ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery

scholarly journals Invasive Techniques in Scar Management: Skin Substitutes

2020 ◽  
pp. 317-323
Author(s):  
F. W. Timmermans ◽  
E. Middelkoop
Keyword(s):  
Wound Healing ◽  
Scar Formation ◽  
Wound Management ◽  
Skin Substitutes ◽  
Current State ◽  
Scar Management ◽  
Scar Quality ◽  
Engineered Skin Substitutes ◽  
Invasive Techniques

AbstractIn the last decades, skin substitutes have emerged as an important innovation in improving scar quality. They can be applied during the initial wound management but also during scar reconstruction procedures. This chapter provides an overview on the development, current state, and future of cell-seeded and tissue-engineered skin substitutes. We will discuss some of the most important varieties of skin substitutes in the context of scar formation and wound healing.

Intra-Uterine and Juxtanatal Repair of Syndactyly in Foetal Mice

1995 ◽  
Vol 20 (3) ◽  
pp. 319-326 ◽  
Author(s):  
J. ROBINSON ◽  
K. C. OBERG ◽  
W. M. KIRSCH ◽  
V. E. WOOD
Keyword(s):  
Wound Healing ◽  
In Utero ◽  
Scar Formation ◽  
Newborn Mice ◽  
The Third ◽  
Digital Necrosis ◽  
The Right

39 foetal mice with genetic syndactyly were identified in utero at 17 days of gestation, and the right hindfoot extruded through the uterus. The syndactylous digits were separated by simple incisions. In one group (n = 25) digit separation was maintained during wound healing by the interdigital application of a silver microclip. Digit separation was also assessed in a second group of newborn mice less than 24 hours old (juxtanatal population, n = 24). Two foetuses (5%) and six newborns (25%) developed digital necrosis following microclip application. In the remaining microchlipped animals (23 intrauterine and 10 juxtanatal), microclip application maintained digit separation, allowing wound healing to occur with epithelialization of the separated digits. No inflammation or scar formation occurred. In the third group (n = 22) without microclip application, the digital skin reapproximated and webbing recurred during wound healing. These studies demonstrate the need to maintain digit separation during wound healing following intra-uterine or juxtanatal syndactyly repair.

scholarly journals The Four-Herb Chinese Medicine ANBP Enhances Wound Healing and Inhibits Scar Formation via Bidirectional Regulation of Transformation Growth Factor Pathway

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e112274 ◽  
Author(s):  
Qian Hou ◽  
Wen-Jun He ◽  
Hao-Jie Hao ◽  
Qing-Wang Han ◽  
Li Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chinese Medicine ◽  
Growth Factor ◽  
Scar Formation ◽  
Bidirectional Regulation

scholarly journals Resveratrol-loaded peptide-hydrogels inhibit scar formation in wound healing through suppressing inflammation

2019 ◽  
Author(s):  
Chen-Chen Zhao ◽  
Lian Zhu ◽  
Zheng Wu ◽  
Rui Yang ◽  
Na Xu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Damage Model ◽  
Skin Wound ◽  
Scar Formation ◽  
Wound Dressings ◽  
Skin Damage ◽  
Skin Wound Healing ◽  
Accelerate Wound Healing ◽  
Peptide Hydrogels

Abstract Scar formation seriously affects the repair of damaged skin especially in adults and the excessive inflammation has been considered as the reason. The self-assembled peptide-hydrogels are ideal biomaterials for skin wound healing due to their similar nanostructure to natural extracellular matrix, hydration environment and serving as drug delivery systems. In our study, resveratrol, a polyphenol compound with anti-inflammatory effect, is loaded into peptide-hydrogel (Fmoc-FFGGRGD) to form a wound dressing (Pep/RES). Resveratrol is slowly released from the hydrogel in situ, and the release amount is controlled by the loading amount. The in vitro cell experiments demonstrate that the Pep/RES has no cytotoxicity and can inhibit the production of pro-inflammatory cytokines of macrophages. The Pep/RES hydrogels are used as wound dressings in rat skin damage model. The results suggest that the Pep/RES dressing can accelerate wound healing rate, exhibit well-organized collagen deposition, reduce inflammation and eventually prevent scar formation. The Pep/RES hydrogels supply a potential product to develop new skin wound dressings for the therapy of skin damage.

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