Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-Term In Vitro Expansion

2021 ◽  
Author(s):  
Anthony J. Berger ◽  
Golnaz Anvari ◽  
Evangelia Bellas
Keyword(s):  
Stem Cell ◽  
In Vitro Expansion ◽  
Mechanical Memory ◽  
Adipose Derived Stem Cell

scholarly journals Multiplex Analysis of Adipose-Derived Stem Cell (ASC) Immunophenotype Adaption to In Vitro Expansion

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 218
Author(s):  
Qiuyue Peng ◽  
Martyna Duda ◽  
Guoqiang Ren ◽  
Zongzhe Xuan ◽  
Cristian Pablo Pennisi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Therapeutic Potential ◽  
Selection Process ◽  
Expression Patterns ◽  
Biological Properties ◽  
Tissue Culture Polystyrene ◽  
In Vitro Expansion ◽  
Unrelated Donors ◽  
Adipose Derived Stem Cell

In order to enhance the therapeutic potential, it is important that sufficient knowledge regarding the dynamic changes of adipose-derived stem cell (ASC) immunophenotypical and biological properties during in vitro growth is available. Consequently, we embarked on a study to follow the evolution of highly defined cell subsets from three unrelated donors in the course of eight passages on tissue culture polystyrene. The co-expression patterns were defined by panels encompassing seven and five cell surface markers, including CD34, CD146, CD166, CD200, CD248, CD271, and CD274 and CD29, CD31, CD36, CD201, and Stro-1, respectively. The analysis was performed using multichromatic flow cytometry. We observed a major paradigm shift, where the CD166-CD34+ combination which was found across all cell subsets early in the culture was replaced by the CD166+ phenotype as the population homogeneity increased with time. At all analysis points, the cultures were dominated by a few major clones that were highly prevalent in most of the donors. The selection process resulted in two predominant clones in the larger panel (CD166+CD34−CD146−CD271− CD274−CD248−CD200− and CD166+CD34+ CD146−CD271−CD274−CD248−CD200−) and one clone in the smaller panel (CD29+CD201+CD36− Stro-1− CD31−). The minor subsets, including CD166+CD34−CD146−CD271+CD274−CD248−CD200− and CD166+CD34+CD146+CD271−CD274−CD248−CD200−, and CD29+CD201−CD36−Stro-1−CD31−, CD29+CD201+CD36−Stro-1+CD31−, and CD29+CD201+CD36+Stro-1−CD31−, in the seven and five marker panels, respectively, were, on the other, hand highly fluctuating and donor-dependent. The results demonstrate that only a limited number of phenotypical repertoires are possible in ASC cultures. Marked differences in their relative occurrence between distinct individuals underscore the need for potency standardization of different ASC preparation to improve the clinical outcome.

scholarly journals Long‐term in vitro expansion ensures increased yield of central memory T cells as perspective for manufacturing challenges

2021 ◽  
Author(s):  
Stefanie Herda ◽  
Andreas Heimann ◽  
Benedikt Obermayer ◽  
Elisa Ciraolo ◽  
Stefanie Althoff ◽  
...  
Keyword(s):  
T Cells ◽  
Memory T Cells ◽  
Central Memory ◽  
Central Memory T Cells ◽  
In Vitro Expansion

3D Human Adipose-Derived Stem Cell Clusters as a Model for In Vitro Fibrosis

2016 ◽  
Vol 22 (7) ◽  
pp. 679-690 ◽  
Author(s):  
Thanavel Rajangam ◽  
Min Hee Park ◽  
Sang-Heon Kim
Keyword(s):  
Stem Cell ◽  
Cell Clusters ◽  
Adipose Derived Stem Cell

Effects Of Hypoxia in Long-Term In Vitro Expansion of Human Bone Marrow Derived Mesenchymal Stem Cells

2017 ◽  
Vol 118 (10) ◽  
pp. 3072-3079 ◽  
Author(s):  
Annelise Pezzi ◽  
Bruna Amorin ◽  
Álvaro Laureano ◽  
Vanessa Valim ◽  
Alice Dahmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vitro Expansion

An in vitro cellular system modelling progressive human adipose-derived stem cell aging

2018 ◽  
Vol 63 (5) ◽  
pp. 272-274 ◽  
Author(s):  
Yanghua Shi ◽  
Lian Wang ◽  
Yichang Li ◽  
Congdi Xu ◽  
Xiaowen Shao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cellular System ◽  
Cell Aging ◽  
System Modelling ◽  
Stem Cell Aging ◽  
Adipose Derived Stem Cell

scholarly journals Cryopreservation of lipoaspirates: in vitro measurement of the viability of adipose‐derived stem cell and lipid peroxidation

2020 ◽  
Vol 17 (5) ◽  
pp. 1282-1290
Author(s):  
Dong Yeon Kim ◽  
Eunjin Kim ◽  
Ki Joo Kim ◽  
Young‐Joon Jun ◽  
Jong‐Won Rhie
Keyword(s):  
Lipid Peroxidation ◽  
Stem Cell ◽  
Adipose Derived Stem Cell

scholarly journals Phthalates affect the in vitro expansion of human hematopoietic stem cell

2019 ◽  
Vol 71 (2) ◽  
pp. 553-561 ◽  
Author(s):  
Ana K. Gutiérrez-García ◽  
José M. Flores-Kelly ◽  
Tomás Ortiz-Rodríguez ◽  
Marco Antonio Kalixto-Sánchez ◽  
Antonio De León-Rodríguez
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
In Vitro Expansion

Structurally Specific Heparan Sulfates Support Primitive Human Hematopoiesis by Formation of a Multimolecular Stem Cell Niche

Blood ◽  
1998 ◽  
Vol 92 (12) ◽  
pp. 4641-4651 ◽  
Author(s):  
Pankaj Gupta ◽  
Theodore R. Oegema ◽  
Joseph J. Brazil ◽  
Arkadiusz Z. Dudek ◽  
Arne Slungaard ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Stem Cell Niche ◽  
Ex Vivo ◽  
Recent Observation ◽  
Hematopoietic Progenitors ◽  
Platelet Factor ◽  
Cell Niche

Abstract Stem cell localization, conservation, and differentiation is believed to occur in niches in the marrow stromal microenvironment. Our recent observation that long-term in vitro human hematopoiesis requires a stromal heparan sulfate proteoglycan (HSPG) led us to hypothesize that such HSPG may orchestrate the formation of the stem cell niche. We compared the structure and function of HS from M2-10B4, a hematopoiesis-supportive cell line, with HS from a nonsupportive cell line, FHS-173-We. Long-term culture-initiating cell (LTC-IC) maintenance was enhanced by PG from supportive cells but not by PG from nonsupportive cells (P < .005). The supportive HS were significantly larger and more highly sulfated than the nonsupportive HS. Specifically, supportive HS contained higher 6-O-sulfation on the glucosamine residues. In agreement with these observations, purified 6-O-sulfated heparin and highly 6-O-sulfated bovine kidney HS similarly maintained LTC-IC. In contrast, completely desulfated heparin, N-sulfated heparin, and unmodified heparin did not support LTC-IC maintenance. Moreover, the supportive HS promoted LTC-IC maintenance but not differentiation of CD34+/HLA-DR−cells into colony-forming cells (CFCs) and mature blood cells. The supportive HS but not the nonsupportive HS bound both cytokines and matrix components critical for hematopoiesis, including interleukin-3 (IL-3), macrophage inflammatory protein-1 (MIP-1), and thrombospondin (TSP). Significantly more CD34+ cells adhered directly to immobilized O-sulfated heparin than to N-sulfated or desulfated heparin. Thus, hematopoiesis-supportive stromal HSPG possessing large, highly 6-O-sulfated HS mediate the juxtaposition of hematopoietic progenitors with stromal cells, specific growth-promoting (IL-3) and growth-inhibitory (MIP-1 and platelet factor 4 [PF4]) cytokines, and extracellular matrix (ECM) proteins such as TSP. We conclude that the structural specificity of stromal HSPG that determines the selective colocalization of cytokines and ECM components leads to the formation of discrete niches, thereby orchestrating the controlled growth and differentiation of stem cells. These findings may have important implications for ex vivo expansion of and gene transfer into primitive hematopoietic progenitors.

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