Cryopreservation of lipoaspirates: in vitro measurement of the viability of adipose‐derived stem cell and lipid peroxidation

Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) and Bio-Oss® bone granules on bone healing in type 2 diabetes mellitus (T2DM) rats with the addition of semaphorin 3A (Sema3A). The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. In addition, Sema3A promoted the osteogenic differentiation of ASC sheets in vitro and local injection of Sema3A promoted T2DM rats’ calvarial bone regeneration based on ASC sheet and Bio-Oss® bone granule complex treatment. In conclusion, the local injection of Sema3A and the complexes of ASC sheet and Bio-Oss® bone granules could promote osseous healing and are potentially useful to improve bone healing for T2DM patients.

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Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-Term In Vitro Expansion

Cellular and Molecular Bioengineering ◽

10.1007/s12195-021-00705-9 ◽

2021 ◽

Author(s):

Anthony J. Berger ◽

Golnaz Anvari ◽

Evangelia Bellas

Keyword(s):

Stem Cell ◽

In Vitro Expansion ◽

Mechanical Memory ◽

Adipose Derived Stem Cell

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The effect of exogenous histone H1 on rat adipose-derived stem cell proliferation, migration, and osteogenic differentiation in vitro

Journal of Cellular Physiology ◽

10.1002/jcp.24042 ◽

2012 ◽

Vol 227 (10) ◽

pp. 3417-3425 ◽

Cited By ~ 14

Author(s):

Li-Wen Hsu ◽

Shigeru Goto ◽

Toshiaki Nakano ◽

Kuang-Den Chen ◽

Chih-Chi Wang ◽

...

Keyword(s):

Cell Proliferation ◽

Stem Cell ◽

Osteogenic Differentiation ◽

Histone H1 ◽

Stem Cell Proliferation ◽

Exogenous Histone ◽

Adipose Derived Stem Cell

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In-vitro cell adhesion and proliferation of adipose derived stem cell on hydroxyapatite composite surfaces

Materials Science and Engineering C ◽

10.1016/j.msec.2017.02.175 ◽

2017 ◽

Vol 75 ◽

pp. 1305-1316 ◽

Cited By ~ 14

Author(s):

Praneetha Pulyala ◽

Akshay Singh ◽

Marcela Ferreira Dias-Netipanyj ◽

Sheron Compos Cogo ◽

Luciane S. Santos ◽

...

Keyword(s):

Stem Cell ◽

Cell Adhesion ◽

Hydroxyapatite Composite ◽

Adipose Derived Stem Cell ◽

Cell Adhesion And Proliferation

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Effects of 3D-bioplotted polycaprolactone scaffold geometry on human adipose-derived stem cell viability and proliferation

Rapid Prototyping Journal ◽

10.1108/rpj-03-2016-0035 ◽

2017 ◽

Vol 23 (3) ◽

pp. 534-542 ◽

Cited By ~ 6

Author(s):

Saahil V. Mehendale ◽

Liliana F. Mellor ◽

Michael A. Taylor ◽

Elizabeth G. Loboa ◽

Rohan A. Shirwaiker

Keyword(s):

Tissue Engineering ◽

Cell Culture ◽

Stem Cell ◽

Pore Size ◽

Compression Modulus ◽

Engineering Applications ◽

Content Type ◽

Musculoskeletal Tissue ◽

Adipose Derived Stem Cell

Purpose This study aims to investigate the effect of three-dimensional (3D)- bioplotted polycaprolactone (PCL) scaffold geometry on the biological and mechanical characteristics of human adipose-derived stem cell (hASC) seeded constructs. Design/methodology/approach Four 3D-bioplotted scaffold disc designs (Ø14.5 × 2 mm) with two levels of strand–pore feature sizes and two strand laydown patterns (0°/90° or 0°/120°/240°) were evaluated for hASC viability, proliferation and construct compressive stiffness after 14 days of in vitro cell culture. Findings Scaffolds with the highest porosity (smaller strand–pore size in 0°/120°/240°) yielded the highest hASC proliferation and viability. Further testing of this design in a 6-mm thick configuration showed that cells were able to penetrate and proliferate throughout the scaffold thickness. The design with the lowest porosity (larger strand–pore size in 0°/90°) had the highest compression modulus after 14 days of culture, but resulted in the lowest hASC viability. The strand laydown pattern by itself did not influence the compression modulus of scaffolds. The 14-day cell culture also did not cause significant changes in compressive properties in any of the four designs. Originality/value hASC hold great potential for musculoskeletal tissue engineering applications because of their relative ease of harvest, abundance and differentiation abilities. This study reports on the effects of 3D-bioplotted scaffold geometry on mechanical and biological characteristics of hASC-seeded PCL constructs. The results provide the basis for future studies which will use this optimal scaffold design to develop constructs for hASC-based osteochondral tissue engineering applications.

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Adipose-derived stem cell-secreted factors promote early stage follicle development in a biomimetic matrix

Biomaterials Science ◽

10.1039/c8bm01253a ◽

2019 ◽

Vol 7 (2) ◽

pp. 571-580 ◽

Cited By ~ 3

Author(s):

Claire E. Tomaszewski ◽

Elizabeth Constance ◽

Melissa M. Lemke ◽

Hong Zhou ◽

Vasantha Padmanabhan ◽

...

Keyword(s):

Stem Cell ◽

Early Stage ◽

Follicle Development ◽

Peg Hydrogels ◽

Secreted Factors ◽

Survival And Growth ◽

Growth Promoting ◽

Adipose Derived Stem Cell

ADSCs encapsulated in biomimetic PEG hydrogels maintain stemness and secrete survival- and growth-promoting factors that support in vitro folliculogenesis.

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